ATF2 전사인자의 발현과 AP-1 전사인자인 BATF, c-Fos, c-Jun과의 이량체 형성

장혜영,김재호,Jang Hye-Young,Kim Jae-Ho 한국생명과학회 2005 생명과학회지 Vol.15 No.6

ATF2는 c-Fos와 c-Jun과 같은 전사인자이며 이들은 루신지퍼 단백질이다. 루신지퍼 단백질은 동형이합체 혹은 이형이합체를 형성하며 promoter 영역에 결합하여 전사조절에 중요한 역할을 한다. 세포의 전사인자 ATF2는 ATF/CRE site에 결합하며 특히 선택적인 interfamily 이형이량체를 형성함으로써 전사 조절에 있어서 다양한 메카니즘을 제공할 수 있다. 본 연구에서는 ATF2 cDNA를 6xHis를 가진 expression vector에 subcloning하여 E.cnli BL2l에서 발현시켰다. 6xHis tag은 nickel-chelating chromatography를 가능하게 하였다 발현된 ATF2는 In vitro binding pull-down assay에서 동형이합체를 이를 뿐만 아니라 AP-1 그룹의 인자들과 선택적인 이형이합체를 형성함을 보여 주었다. BATF와는 강하게 결합하였으며 c-Jun과도 안정된 이합체를 형성하였다. 그러나 c-Fos와는 이합체를 형성하지 않으므로서 AP-1그룹 내에서도 이합체 형성이 선택적으로 이루어짐을 알 수 있다. ATF2 is a cellular transcription factor which belongs to the CREB/ATF class and it is leucine zipper protein which generally binds to DNA as dimers. This paper presents the procedure for subcloning the ATF2 gene and the results of experiment used the expressed ATF2. The pET expression vector was used since it produced 6xHis fusion protein for easy purification using affinity column. The Nickel chelating chromatography was used for Purifying the expressed ATE2 from E- codi BL2l. Subsequen시y In vitro binding pull-down assay showed the binding specificity of ATF2 with AP-1 family factors such as BATF, c-Fos, c-Jun and ATF2 itselgf. ATF2 forms homodimer as well as strong heterodimer with BATF. It also forms stable dimer with c-Jun but barely binds with c-Fos.

분만장 간호사의 사산아와 부모 돌봄 경험

장혜영,정재원,Jang, Hye Young,Chung, Chae Weon 한국여성건강간호학회 2017 여성건강간호학회지 Vol.23 No.3

Purpose: The purpose of the study was to have an in-depth understanding of delivery room nurses' experiences of caring for stillborn babies and their parents. Methods: After obtaining approval from the Institutional Review Board, data were collected from March 1 to August 30, 2016. A total of six nurses participated in the study. Semi-structured in-depth interviews were conducted and the data subsequently analyzed. Results: Using thematic analysis, six themes were defined and refined: Emotional distress as a result of encountering stillborn babies; Caring for the deceased baby; Concerns for the mother's pain; Consideration for the father; Conflicts between accepting and dismissing parents' requests; and Strong mindset for the stillborn babies and their parents. Conclusion: The results showed that the nurses' experiences of caring for stillborn babies and their parents might be a difficult and lonely process. However, it could also be a process of attaining maturity by finding meaning and value in one's work. It would be helpful for the delivery room nurses to share examples of effective communication, train through simulation, and learn useful strategies that each nurse can use.

GPS를 이용한 LED 동기점멸등 제어 및 충방전 시스템

장혜영(Hye Young Jang),손영대(Young Dae Son),김철진(Cherl Jin Kim) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.10

For a vessel to safely navigate on the ocean, it is required to check the vessel position as frequently as possible. When a vessel navigates on the ocean or entering/leaving a port, the target area such as islands, points and mountain peak, etc. are used. However, when such target are not available or when vessel navigates in night time, installed artificial navigation aid facilities help successful vessel navigation. Light Wave Aid helps to confirm a position to the navigator directly and quickly. So, the synchronous light to display the accurate signal has wide applications. However, lantern domestic industry is small, and most of the products show non-compatibility. Consequently, this paper presents the synchronous lighting using UTC of GPS, which maintains compatibility with different lanterns. Also, by replacing conventional electric bulb with LED lamp, simple and efficient lighting system is obtained.

노인 고혈압환자의 미충족 의료에 영향을 미치는 요인

장혜영(Hye young Jang) 한국컴퓨터정보학회 2021 韓國컴퓨터情報學會論文誌 Vol.26 No.11

본 연구는 노인 고혈압환자의 미충족의료에 영향을 미치는 요인을 조사하는 연구이다. 본 연구의 목적은 65세 이상의 노인 고혈압 환자의 미충족의료에 대한 이유를 파악하고 유형별 특성을 확인해 미충족의료가 발생하지 않도록 의료이용 제도를 개선하고 정책 결정을 하는 데 기여하고자 실시하였다. 국민건강영양조사 제 7기(2016, 2017년) 자료를 이용하여 적절가구 중 계통추출법을 이용하여 23개 표본가구를 선정하였으며 SPSS 18.0 Program을 이용하여 분석하였다. 연구결과 남성보다 여성이, 주관적 건강상태는 나쁠수록, 민간보험에 가입한 사람보다 가입하지 않은 사람, 흡연 경험은 있을 때, 최근 1년간 자살생각이 없었던 사람에 비해 자살생각이 있었던 사람의 미충족의료 경험률이 높게 나타났다 이는 향후 미충족의료가 발생하지 않도록 의료이용 제도를 개선하고 정책 결정을 하는 데 기여할 수 있을 것으로 사료된다. The purpose of this study is to investigate factors affecting unmet medical care in elderly hypertensive patients. The purpose of this study was to understand the reasons for unmet medical care in elderly hypertensive patients over 65 years of age and to identify the characteristics of each type to improve the medical use system and to contribute to policy making so that unmet medical care does not occur. Using data from the 7th period of the National Health and Nutrition Examination Survey (2016, 2017), 23 sample households were selected using the phylogenetic extraction method among appropriate households and analyzed using the SPSS 18.0 Program. As a result of the study, the unmet medical care of those who had suicidal thoughts compared to those who had not had suicidal thoughts in the past year, when females were higher than males, had worse subjective health status, those who did not subscribe than those who had private insurance, and those who had smoked in the past year. The experience rate was high. This is considered to be able to contribute to improving the medical service system and making policy decisions so that unmet medical care does not occur in the future.

우리나라 성인 당뇨병 환자의 미충족의료 관련 요인

장혜영 ( Hye Young Jang ),한미아 ( Mi Ah Han ),박종 ( Jong Park ) 한국보건행정학회 2021 보건행정학회지 Vol.31 No.3

Background: This study was conducted to investigate factors related to unmet medical needs of medical care in adult diabetes patients and to suggest factors related to unmet medical in Korea. Methods: This study used data from the Korea National Health and Nutrition Examination Survey (KNHNES) 2014-2017. The subjects of the study were conducted on patients with unmet medical needs experience among the patients and analyzed using the IBM SPSS ver. 25.0 program (IBM Corp., Armonk, NY, USA). Results: Overall, 10.9% of patients had unmet medical needs. Being female, less educated, and lower medical aid were related to unmet medical needs. And subjects with poor subjective health and higher stress level were more likely to report unmet medical needs. Conclusion: Although comprehensive health insurance coverage, 10.9% of people with diabetes experienced unmet healthcare needs. The results of this study suggest that socioeconomic factors such as low education and medical aid were associated with unmet medical needs.

남성 독거노인의 영양장애 예측요인

장혜영(Hye-Young Jang),김지혜(Ji-Hye Kim) 한국자료분석학회 2020 Journal of the Korean Data Analysis Society Vol.22 No.3

남성 독거노인은 성별과 독거라는 거주 형태에서 야기된 영양장애의 위험요인을 동시에 보유한 취약 집단이다. 본 연구는 남성 독거노인의 영양장애 예측요인을 파악하고자 수행되었다. 본 연구는 2017년 노인실태조사 자료를 이용한 이차분석연구이며, 65세 이상 남성 독거노인 455명의 자료를 빈도분석, 카이제곱 검정, t 검정, 다변량 로지스틱 회귀분석을 하였다. 연구결과 남성독거노인의 영양 상태 평균점수는 21점 만점 중 5.37±3.44점이었고, 영양장애 위험군은 78.9%로 나타났다. 대상자의 영양 상태에 따른 주요 변수의 차이를 검증한 결과 교육수준(t=8.97, p=.011), 경제 상태(t=16.85, p=.002), 과음(x2=6.88, p=.009), 만성질환 수(t=-5.98, p<.001), 복용약물 수(t=-4.82, p<.001), 지각된 건강상태(x2=28.33, p<.001), 우울(x2=16.59, p<.001), 인지기능(t=4.46, p<.001), 사회활동참여(t=4.22, p<.001), IADL 제한(x2=4.39, p=.036)에서 유의한 차이를 보였다. 영양장애 예측요인으로는 과음 여부(OR 3.799, 95% CI:1.852-7.792), 지각된 건강상태(OR 2.441, 95% CI:1.158-5.145), 만성질환 수(OR 1.361, 95% CI:1.105-1.677), 인지기능(OR 0.880, 95% CI:0.801-0.967), 사회활동참여(OR 0.862, 95% CI:0.793-0.937)가 확인되었다. 따라서 남성 독거노인의 영양장애를 예방하기 위한 방안 마련 시 건강행태를 개선하고, 지속적인 만성질환 관리뿐만 아니라 인지기능과 사회활동 참여를 증진시키기 위한 전략이 고려되어야 할 것이다. The purpose of this study was to identify the factors influencing the malnutrition in the elderly men living alone in Korea. This study analyzed the data of 455 elderly men living alone using the 2017 National Survey of the Living Conditions of Korean Elderly. As a result of the study, the average score of the nutritional status was 5.37±3.44 out of 21, and the risk group of malnutrition was 78.9%. Education level, economic status, heavy drinking, number of chronic diseases, number of medications, subjective health status, depressive symptoms, cognitive function, social activity, and IADL limitation showed significant differences according to the nutritional status of the subjects. Malnutrition was influenced by heavy drinking (OR 3.799, 95% CI:1.852-7.792), subjective health status (OR 2.441, 95% CI:1.158-5.145), number of chronic diseases (OR 1.361, 95 % CI: 1.105-1.677), cognitive function (OR 0.880, 95% CI: 0.801-0.967), and social activity (OR 0.862, 95% CI: 0.793-0.937). Therefore, to develop malnutrition prevention program for the elderly men living alone, it would be considered to improve health behaviors, manage chronic diseases as well as promote cognitive function and participation in social activities.

생명체의 적응능력, 또 하나의 설계의 증거

장혜영(Hye Young Jang) 창조론오픈포럼 2012 창조론오픈포럼 Vol.6 No.1

Life forms share a amazingly high level of adaptability. This is evidence that God almighty with supernatural powers has been behind creation. The ability to adapt is less about the mere end goal to evolve itself into different species, but rather as a defense mechanism to sustain itself-- part of an intellectual blueprint bestowed by God. The diversity that exists in all life, throughout a number of different environments-- is represented by the uniqueness in every species, all varied in their subtle ways. The goal of this article is to focus on the adaptability of life forms, how they respond and how they adapt to different temperatures. Also the adaptation to DPG, high altitude environments, the effect of epigenetics, the adaptation and metamorphosis in form will also be covered.

토론 교육 관련 연구 흐름 분석을 통한 대학 토론 교육 의미 재고

장혜영(Jang, Hye-Young) 한국수사학회 2016 수사학 Vol.0 No.27

The study starts from the basic questions on debate education in University. What do we need to teach them? How do we deliver them? During last fifteen years, there have been lots of progress on education related debate in Korean society. Despite academic accumulations on this subject, we easily can find that discrepancy and discord in the concept and jargon on debate among researchers. This study will review the papers related to debate pedagogy which was published last fifteen years. Through this review process, the inherent problems will be elicited in debate courses in University education. Finally, the new direction and methods concerning debate courses will be suggested. One hundred and thirty papers related to debate education were classified as followings; debate history, debate concept, the current status and prospect of debate(speech) education, debate capacity, debate course design, debate course case study, debate pedagogy, debate type, debate evaluation, debate tournaments, reading and debate. Above mentioned papers are diverse in terms of academic institute and they are come from 40 institutes as well as various academic disciplines. There are conflicting opinions toward the objective of debate education which each researcher suggested. This confusion might be due to the conceptual difference on the idea on debate. The word, ‘토론’ in korean is being used as a simple translation of debate. However, it compasses the broad meaning of discussion. There might be the intrinsic characteristics in Korean debate culture; there is a prevailing anti attitude competitive debate and pro attitude cooperative discussion attitude on the other hand. By analysing the various education objectives, we could find the two different tendencies. When 토론 was used in the narrow meaning of debate, the objective tends to the improvement of debate ability. On the other hand, the democracy citizen nurturing is the common objective in the case of the education objective used as the broad meaning of 토론. The study suggests some practical solutions. There is a need on unifying of concept and word in debate education. We need to separate objectves and goals, debate and debate education. And we need to set different objective on each different course. Up to now, diverse agencies contributed in developing debate education. There is a strong need of association and institute encompassing debate education and research. Promoting the umbrella agency will contrive to developing the debate education.

17세기 프랑스 희극작품들에 사용된 거짓말

장혜영(Hye-Young Jang) 프랑스문화예술학회 2009 프랑스문화예술연구 Vol.28 No.-

신문 기사를 통한 제19대 대통령 선거 후보자 TV 토론회 분석

장혜영(Jang, Hye Young) 한국수사학회 2018 수사학 Vol.0 No.31

The TV debates for the 19th South Korean Presidential election were conducted in various formats; Time-limited Debate, Initiativecontrolled Debate, 1:1 Policy Debate, Standing Debate, Round-table Debate, Presentation, etc. Though TV debates are known to reinforce the conformation bias of electors, the last debates caused the approval ratings of candidates to fluctuate. The aim of this study is to examine each of the debate formats that were conducted on the last Presidential Debate. Newspaper articles were chosen for their subject of analysis, since the reproduction of articles are as important as the debate itself. This study mainly focuses on evaluating the debate formats. Comparative analysis between news organizations has also been conducted. The 19th South Korean Presidential Election Debate media report coverage published by the National Election Commission, is the main subject of analysis. 366 news reports of major press media, from April to May, 2017 were also analyzed. Both quantitative and qualitative measurements were taken. Text mining through R was conducted, and qualitative research has been taken. Debate format, number of debaters, selection of topics, time management, environmental background setup and the role of the host have also been taken into consideration. Improvement points of the debate have been sought, while the importance of the debate on values, not on policies, has been reviewed.