국제 사료원료시장 변화에 따른 구매역할의 재조명

이회영,Lee, Hoe-Yeong 한국사료협회 2009 飼料 Vol.37 No.-

獨逸 古典主義 文學과 浪漫文學

이회영 성균관대학교 독어독문학회 1976 獨逸文學論考 Vol.5 No.1

獨逸古典主義 文學과 浪漫文學 硏究에 對한 第三命題 : 救濟와 解脫

李檜永 성균관대학교 독어독문학회 1976 獨逸文學論考 Vol.5 No.1

흰쥐 기관평활근에 대한 GS 386의 칼슘억제 및 포스포디에스테라제 억제 작용

장기철,이회영,강영진,구의본 大韓藥理學會 1996 대한약리학잡지 Vol.32 No.3

최근 본 연구실에서는 GS 386인 1-(4'-methoxybenzyl)-6,7-dimethoxy-3,4-dihydroisoquinoline이 적출된 토끼의 심방세포에서 <TEX>$Ca^{++}$</TEX> 채널의 운동성 변화없이 <TEX>$Ca^{++}$</TEX> 채널이 열릴 가능성을 줄임으로써 <TEX>$Ca^{++}$</TEX> 전류의 증폭을 억제한다고 보고하였다. 이번 연구에서는 적출된 쥐의 기관지를 사용하여 GS 386의 작용기전에 대해 연구하였다. GS386은 carbachol <TEX>$(0.3{\mu}M)$</TEX>과 높은 농도의 <TEX>$K^+$</TEX> (65.4mM)에 의해 수축된 쥐의 기관지를 용량-의존적으로 이완시켰으며 이때 <TEX>$IC_{50}$</TEX>는 5.24와 <TEX>$5.67\;{\mu}M$</TEX>이었다. verapamil은 carbachol에 의한 수축시 보다 높은 농도의 <TEX>$K^+$</TEX>에 의해 수축된 조직에 더욱 효과적으로 억제하였다. <TEX>$Ca^{++}$</TEX>이 없는 상태에서 <TEX>$Ca^{++}$</TEX>에 의한 수축은 GS386에 의해 억제되었다. 더욱이 높은 농도의 GS386<TEX>$(100\;{\mu}M)$</TEX>은 verapamil과는 다르게 carbachol뿐만 아니라 caffeine에 의한 위상성 수축을 억제 시키므로 GS386은 세포질내로 들어가 sarcoplasmic retuculum과 같은 근육 내부에 2차적인 영향을 나타내었다. 더군다나GS386은 verapamil에 의해 영향을 받지않는 (verapamil-insensitive component)이완을 보였고 쥐 기관지의 평활근에서 cAMP의 양을 증가 시켰다. 이러한 결과는 GS386의 작용기전이 <TEX>$Ca^{++}$</TEX> 길항적인 작용 뿐만 아니라 posphodiesterase억제작용에 기인한다는 사실을 제시한다. Recently we reported that GS 386, 1-(4'-methoxybenzyl)-6,7-dimethoxy-3,4-dihydroisoquinoline, inhibited amplitude of the <TEX>$Ca^{2+}$</TEX> current by reducing the probability of <TEX>$Ca^{2+}$</TEX> channel opening without changing channel kinetics in isolated rabbit atrial myocyte. In the present study, further investigation of the mechanism of action of GS 386 was performed using isolated rat trachealis. GS 386 concentration-dependently relaxed rat trachealis contracted by carbachol <TEX>$(0.3{\mu}M)$</TEX> and high <TEX>$K^+$</TEX>(65.4 mM) with <TEX>$IC_{50}$</TEX> 5.24 and 5.67 <TEX>${\mu}M$</TEX>, respectively. Verapamil inhibited more effectively the high <TEX>$K^+-contracted$</TEX> tissues than those with carbachol in the rat trachealis muscle. In <TEX>$Ca^{2+}-free$</TEX> media, <TEX>$Ca^{2+}-induced$</TEX> contraction was inhibited by GS 386. Furthermore, high concentration of GS 386 <TEX>$(100{\mu}M)$</TEX> but not verapamil, attenuated a phasic contraction induced not only by carbachol but also caffeine, indicating that GS386 can enter into the cytoplasm where it may exert secondary actions on internal sites of the muscle, such as sarcoplasmic reticulum. Moreover, GS 386 showed verapamil-resistant component of relaxation and increased cAMP levels in rat trachal smooth muscle. These results suggest that the mechanism of action of GS 386 attributes to not only <TEX>$Ca^{2+}$</TEX> antagonistic action but also weak phosphodiesterase inhibitory action.

膵臟 組織내 Protein Methylase I 酵素의 精製

李香雨,李晦榮 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

An enzyme which methylates the guanido group of arginine residues of protein using S-adenosyl-L-methionine as methyl donor has been designated as protein methylase I. Among the enzyme products of this reaction which thus far have been identified are N^G-mono, N^G,N^c-di-, and N^G, N'^G- dimethylarginine. This enzyme is widely distributed in various organs of rat, being especially rich in pancreas of hog. The level of protein methylase I activity was found to closely parallel the rate of cell proliferation. Elevated activity was observed in fetal tissues, continuously dividing HeLa S-3 cell culture, regenerating adult rat liver, and in fast growing Novikoff hepatoma. Protein merhylase I has been purified from calf brain, rat and calf thymus, Krebs II ascites cells and recently a form of protein merhylase I has also been purified from wheat germ, which is the first report of the enzyme being present in the plant kingdom. In the present paper, protein methylase I has been partially purified from hog pancreatic tissue. 1) Protein methylase I has been partially purified about 14-fold from hog pancreatic tissue with 25% yield. 2) Mainly the enzyme was found in cytosolic fraction of pancreatic cells. 3) Main purification steps were homogenation of the tissue, S_3 fraction of cells, 35% ammonium sulfate ppt., and DIEM-cellulose chromatography.

Calcium Channel Blocking and Phosphodiesterase Inhibitory Action of GS386, a Dihydroisoquinoline Derivative, in Isolated Rat Trachea

장기철,이회영,강영진,구의본,Chang, Ki-Churl,Lee, Hoi-Young,Kang, Young-Jin,Koo, Eui-Bon The Korean Society of Pharmacology 1996 대한약리학잡지 Vol.32 No.3

최근 본 연구실에서는 GS 386인 1-(4'-methoxybenzyl)-6,7-dimethoxy-3,4-dihydroisoquinoline이 적출된 토끼의 심방세포에서 $Ca^{++}$ 채널의 운동성 변화없이 $Ca^{++}$ 채널이 열릴 가능성을 줄임으로써 $Ca^{++}$ 전류의 증폭을 억제한다고 보고하였다. 이번 연구에서는 적출된 쥐의 기관지를 사용하여 GS 386의 작용기전에 대해 연구하였다. GS386은 carbachol $(0.3{\mu}M)$과 높은 농도의 $K^+$ (65.4mM)에 의해 수축된 쥐의 기관지를 용량-의존적으로 이완시켰으며 이때 $IC_{50}$는 5.24와 $5.67\;{\mu}M$이었다. verapamil은 carbachol에 의한 수축시 보다 높은 농도의 $K^+$에 의해 수축된 조직에 더욱 효과적으로 억제하였다. $Ca^{++}$이 없는 상태에서 $Ca^{++}$에 의한 수축은 GS386에 의해 억제되었다. 더욱이 높은 농도의 GS386$(100\;{\mu}M)$은 verapamil과는 다르게 carbachol뿐만 아니라 caffeine에 의한 위상성 수축을 억제 시키므로 GS386은 세포질내로 들어가 sarcoplasmic retuculum과 같은 근육 내부에 2차적인 영향을 나타내었다. 더군다나GS386은 verapamil에 의해 영향을 받지않는 (verapamil-insensitive component)이완을 보였고 쥐 기관지의 평활근에서 cAMP의 양을 증가 시켰다. 이러한 결과는 GS386의 작용기전이 $Ca^{++}$ 길항적인 작용 뿐만 아니라 posphodiesterase억제작용에 기인한다는 사실을 제시한다. Recently we reported that GS 386, 1-(4'-methoxybenzyl)-6,7-dimethoxy-3,4-dihydroisoquinoline, inhibited amplitude of the $Ca^{2+}$ current by reducing the probability of $Ca^{2+}$ channel opening without changing channel kinetics in isolated rabbit atrial myocyte. In the present study, further investigation of the mechanism of action of GS 386 was performed using isolated rat trachealis. GS 386 concentration-dependently relaxed rat trachealis contracted by carbachol $(0.3{\mu}M)$ and high $K^+$(65.4 mM) with $IC_{50}$ 5.24 and 5.67 ${\mu}M$, respectively. Verapamil inhibited more effectively the high $K^+-contracted$ tissues than those with carbachol in the rat trachealis muscle. In $Ca^{2+}-free$ media, $Ca^{2+}-induced$ contraction was inhibited by GS 386. Furthermore, high concentration of GS 386 $(100{\mu}M)$ but not verapamil, attenuated a phasic contraction induced not only by carbachol but also caffeine, indicating that GS386 can enter into the cytoplasm where it may exert secondary actions on internal sites of the muscle, such as sarcoplasmic reticulum. Moreover, GS 386 showed verapamil-resistant component of relaxation and increased cAMP levels in rat trachal smooth muscle. These results suggest that the mechanism of action of GS 386 attributes to not only $Ca^{2+}$ antagonistic action but also weak phosphodiesterase inhibitory action.

돼지 간장 조직내 Protein Methylase Ⅱ 저해제의 정제 및 특성

권명희,정기경,이회영,이향우,홍성렬 ( Myung Hee Kwon,Ki Kyung Jung Hoi,Young Lee,Hyang Woo Lee,Sungyoul Hong ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.6

An inhibitor for protein methylase II (EC 2.1.1.24) was solubilized from porcine liver microsomal fraction by heat treatment, and purified by ultrafiltration, Sephadex G-25 chromatogrnphy, and HPLC using μ-Bondapak C_(18) column. The purified inhibitor was near homogeniety as judged by HPLC. The molecular weight of the inhibitor was estimated to be 1,676 Da by analysis of amino acid composition. And it was revealed that the inhibitor molecule is rich in alanine and glycine. The activity of the inhibitor was not affected by heat treatment up to 100℃ as well as hydrolytic enzymes. The K; value for the protein methylase II which has been purified from porcine spleen was measured to be 1.3 × 10^(-7) M. Inhibition studies showed that the inhibitor was noncompetitive with respect to S-adenosyl-L-methionine (SAM) and activities of several SAM-dependent methylases were also inhibited by the purified inhibitor.

Nitric Oxide Modulates Calcium Current in Cardiac Myocytes but not in Intact Atrial Tissues

박춘옥,강영진,이회영,장기철,Park, Choon-Ok,Kang, Young-Jin,Lee, Hoi-Young,Chang, Ki-Churl The Korean Society of Pharmacology 1995 대한약리학잡지 Vol.31 No.3

본 연구의 목적은 외부에서 nitric oxide (NO)를 투여 하였을때 심근 수축력, 심박동수의 변화 및 혈관 평활근에 대한 효과를 비교함으로서 NO에 대한 이들 장기의 민감도가 서로 같은지 또는 상이한지를 알아보고자 하였다. 본 실험에서는 PIANO 방법에 의한 근장력의 변화와 아울러 심근에서의 $Ca^{2+}$ current를 측정하였다. 랫트의 심방근에 대한 PIANO $(STZ,\;100\;{\mu}M)$는 심근수축력 및 심박동수에 전혀 변화를 주지 않았지만 혈관 평활근에서는 강한 이완 작용을 나타내었다. 한편, 8-Br-cGMP도 고농도 $(100\;{\mu}M)$에서만 심근 수축력을 억제하였다. 토끼의 심방근세포에서 Whole cell voltage patch clamp를 사용시 bradykinin, SNP, 8-Br-cGMP 및 PIANO는 $Ca^{2+}$ current를 억제하였다. 이러한 사실은 외부에서 공급되는 NO에 대한 심근과 혈관 평활근의 반응에는 민감도의 차이가 있음을 암시하며 더 나아가 심근의 경우에도 NO 반응에는 종 (species)간의 차이와 동일 종이라 하더라도 세포(cell)와 장기(tissue)에 차이가 있을 가능성을 제시하였다. The aim of the present study was to know whether exogenously administered nitric oxide (NO) may differently modulate muscle mechanics between heart and aorta. We used PIANO method to generate NO. In isolated rat atrial tissues, neither heart rate nor contractility was affected by PIANO $(STZ,\;30{\sim}100\;{\mu}M)$. Only high concentration $(100\;{\mu}M)$ of 8-bromo cyclic GMP slightly depressed cardiac contractility. However, the same concentrations of 8-Br cGMP and PIANO significantly relaxed the rat thoracic aorta contracted with phenylephrine $(0.1\;{\mu}M)$. In isolated rabbit cardiac atrial myocytes, the amplitude of calcium currents were decreased in the whole voltage range by the presence of streptozotocin, which was further potentiated by UV light. Calcium currents were also decreased in those preparations treated with bradykinin, nitroprusside and 8-Br cGMP. These findings suggest that exogenous NO may modulate calcium current in cardiac myocyte. However, it remains why this does not affect myocardial contractility and heart rate. We concluded that NO may differently regulate calcium signal between aorta and heart muscle.

관상동맥이완과 혈소판응집에 대한 GS283 과 GS386 의 약리작용기전에 관한 연구

장기철(Ki Churl Chang),이회영(Hoi Young Lee),이균우(Goun Woo Lee),구의본(Eui Bon Koo),강영진(Young Jin Kang),이영수(Young Soo Lee) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

Trimetoquinol (TMQ) and its analogs are known to have thromboxane A₂ antagonistic action. We also reported that GS389, chemically similar to TMQ, has competitive antagonistic action in rat aorta and human platelets. In the present study, we investigated the pharmacological characteristics of GS283 and GS386, analogs of GS389, using vascular smooth muscle, human platelets and rat brain homogenates. In isolated pig coronary artery (PCA), both of GS283 and GS386 relaxed U46619-contracted rings in concentration dependent manner. Pretreatment with several concentrations of GS283 and GS386 shifted the dose-response curves to the right, and reduced of maximum contration dose-dependently. Furthermore, GS283 and GS386 strongly inhibited Ca^(2+)-induced contraction in the PCA. In human platelets, U46619- and A23187-induced platelet aggregation was inhibited by GS283 and GS386, concentration-dependently. Anti-platelet aggregation was related to the compound`s ability to inhibit ATP release at each stimulation. In rat brain homogenates, receptor-binding assay resulted that both GS283 and GS386 have a relative affinity to α-adrenergic receptor. Taken together, we concluded that the mechamism of action of GS283 and GS386 is not related with in TXA₂ receptor but concerned with calcium antagonistic action and α-blocking action.

말의 활액 및 혈청 분석을 통한 관절질환의 생화학적 예측 인자

배종환,김갑수,유영춘,이회영,이경복 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.3

The joint disease including osteoarthris (OA) and rheumatoid arthritis (RA) are common in the horse. Many studies have been performed to develop biochemical markers reflecting the abnormalities of cartilage and synovial membrane. However, no specific, sensitive and clinically well established assay systems have been yet available to characterize the severity of joint diseases. Indeed, radiography is still doctor's best choice of assessing joint damage in OA/RA. This review focuses on biochemical molecules such as proteoglycan, collagen, matrix metalloproteinases (MMPs), lectin and cytokine to assess their potential value for not only predicting stage of joint disease but also monitoring treatment efficacy.