http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
한국산(韓國産) 광대싸리(Securinega suffruticosa Rehder)에서의 Securinine 추출(抽出)
이춘영,이성환,안학수,Lee, Chun-Yeong,Lee, Seong-Hwan,An, Hak-Su 한국응용생명화학회 1965 Applied Biological Chemistry (Appl Biol Chem) Vol.6 No.1
(1) 한국중부산(韓國中部産) 광대싸리(Securinega suffruticosa Rohder)에 함유(含有)되는 식물성염기(植物性鹽基) Securinine의 추출(抽出)을 기도(企圖)하였다. (2) 한국원내식물분포도(寒國內源植物分布圖)는 극동지역(極東地域) 제일(第一)의 생태피도(生態被度)를 갖는 것으로 생각(生覺)된다. (3) 융점(融點)과 외부형태(外部形態)(현미경적(顯微鏡的)) 그리고 기타(其他)를 종합고찰(綜合考察)하여 추출물(抽出物)이 Securinine이라는 것을 확인(確認)하였다. (4) 수율(收率)을 평균(平均) 0.075% 였고 낙엽직전(落葉直前)의 황엽(黃葉)이란 점(點)이 함량(含量)을 감소(減少)케한 원인(原因)으로 생각(生覺)되었으며 하기생육(夏期生育)이 증가(增加)한 시기(時期)를 골라 재추출(再抽出)을 하면 상당(相當)한 수율(收率)의 증가(增加)를 가져올 것으로 추측(推測)되었다. (5) 수지상(樹脂狀) 점조물(粘稠物) 함유(含有) alkaloide 간(間)의 분리(分離)에는 새로운 용매(溶媒)의 선택(選擇)과 조작(操作)을 강구(講究)하여야 할것으로 생각(生覺)된다. (6) 추출과정(抽出過程)은 다소변경(多少變更)하였으며 앞으로 더욱 간편(簡便)하고 고수율(高收率)인 방법(方法)이 강구(講究)될 가능성(可能性)은 찾을수 있었다. 1. The extraction of securine, an alkaloid, from Securinega suffruticosa Rehder growing in Korea was investigated. 2. The distribution of the source plant in Korea is considered to be most intense among the Fareast countries. 3. The extracted material was identified to be genuine securinine viewing from melting point and microscopic observation. 4. The yield of the substance in this study was 0.075% in average. The less amount of recovery may be due to the collection of the plant in October instead of that in the prime season. 5. There should be more efficient way in the separation of resinous impurities from the crude product. Application of Chromatographic technique and selection of more efficient solvent will be investigated hereafter.
생약제제 중 이산화황 잔류실태 시중 유통 생약제제 중 이산화황의 잔류실태 조사
이춘영(Chun-Yeong Lee),이정숙(Jeong-Sook Lee),이현경(Hyun-Kyung Lee),박원희(Won-Hee Park),김정곤(Jeong-Gon Kim),정애희(Ae-Hee Chung) 대한약학회 2012 약학회지 Vol.56 No.1
This study was carried out to investigate the content of sulfur dioxide residues in the medicine prepared from crue drugs. Total 29 pills that contains salvia miltiorrhiza root, anemarrhena rhizome, fritillaria bulb, glehnia root, asparagus tuber, lycium fruit, moutan root bark, cimicifuga rhizome, pinellia tuber, dipsaci radix, codonopsis pilosula root, liriope tuber, achyranthes root, prepared aconite, aurantii fructus immaturus, corydalis tuber, dioscorea rhizome were examined by the modified Monier_Willams method. Sulfur dioxide were detectd in all the tested samples, and were found over MRLs (30 mg/kg to medicinal herbs) in 8 samples (27.6%). Especilly, "i2" pills contains 132.46 mg/kg sulfur dioxide that is 4 times over than MRLs.
정지흔,조백현,이춘영,Jeong, Ji-Heun,Jo, Baek-Hyeon,Lee, Chun-Yeong 한국응용생명화학회 1963 Applied Biological Chemistry (Appl Biol Chem) Vol.4 No.1
한국(韓國)고추장 십종(十種)(주(主)로 전라도산(全羅道産)의 일반성분(一般性分)과 카로틴 비타민$B_2$ 비타민 C 그리고 신미성분(辛味成分) 갑사이신의 함량(含量)을 분석(分析)하였다.
새로운 플루오로 퀴놀론계 항균제에 대한 내성 MRSA 균주의 In Vitro 선발과 그 내성 기전 분석
윤은정,김현지,이춘영,최응칠,심미자,Yoon, Eun-Jeong,Kim, Hyun-Jee,Lee, Chun-Yeong,Choi, Eung-Chil,Shim, Mi-Ja 대한약학회 2008 약학회지 Vol.52 No.3
Clinically isolated methicillin-resistant Staphylococcus aureus strains were exposed to subinhibitory concentration of DW286, DW-224a, gemifloxacin, trovafloxacin, sparfloxacin and ciprofloxacin during 26- to 39-days period. Subculturing led to resistance development, and most of the selected mutants were above susceptible breakpoints. Selected mutants had broad cross resistance to other quinolone antibiotics and only one mutant was completely susceptible to all fluoroquinolones. Twenty five among 42 mutants revealed mutations on DNA gyrase and topoisomerase IV by sequencing. Also 16 mutants had fluoroquinolones MICs that were 4-32 times lower in the presence of reserpine. In conclusion, alterations in DNA gyrase or topoisomerase IV and action of efflux pumping out system are the resistance mechanisms of DW-224a.
숙성(熟成) 진석화(眞石花)젓의 당(糖) 및 유리(遊離) 아미노산(酸)에 관(關)하여
김형수,김만조,이춘영,Kim, Hyeong-Su,Kim, Man-Jo,Lee, Chun-Yeong 한국응용생명화학회 1964 Applied Biological Chemistry (Appl Biol Chem) Vol.5 No.1
1) 진석화(眞石花)젓은 그 수분함량(水分含量)이 생(生)굴에 비(比)하여 상당(相當)히 감소(減少)하여 농축(濃縮)된 상태(狀態)이며, glycogen의 함량(含量)도 격감(激減) 하였음에 반(反)하여 유리당(遊離糖)은 현저(顯著)하게 증가(增加)되었다. 2) Paper chromatography로 시료(試料) 즙액중(汁液中)에서 검출(檢出) 확인(確認)된 유리(遊離) 아미노산(酸)는 aspartic acid, glutamic acid, cystine, serine, glycine, lysine, threonine histidine, alanine, arginine, valine, methionine, tyrosine, phenylalanine, leucine, iso-leucine, tryptophan 등 17종(種)이 였으며, 영양상(榮養上) 필수(必須) amino acid를 전부(全部) 함유(含有)하고 있었다. 3) 비색정량법(比色定量法)에 의(依)해서 정량(定量)된 시료(試料) 즙액중(汁液中) 유리(遊離) 아미노산(酸)의 함량(含量)은 Tyr. 0.264mg/ml, Arg. 0.304mg/ml, Try. 0.046mg/ml, His. 0.460mg/ml, Met. 0.350mg/ml, Cys. 0.440mg/ml, phe. 0.115mg/ml 이었다. 1) The Moisture and glycogen contents of salted-oyster (Zin Suk Hwa Jut) were much less than those of raw oyster, while the free sugar content was dominent. 2) The amino acids were detected and identified as aspartic acid, glutamic acid, cystine, serine, glycine, lysine, threonine, histidine, alanine, arginine, valine, methionine, tyrosine, phenylalanine, leucine, iso-leucine and tryptophan in the juice of salted-oyster by using the method of paper chromatography, and the all essential amino acids from nutritional view point was admitted in this juice. 3) The juice contained 0.264mg/ml of tyrosine, 0.304mg/ml of arginine, 0.046mg/ml of tryptophan, 0.460mg/ml of histidine, 0.350mg/ml of methionine, 0.440mg/ml of cystine and 0.115mg/ml of phenylalanine.
Antilipolytic Activity를 보유하는 인삼 Oligopeptide의 추출 및 정제
김수일,나지영,조도현,이춘영,Kim, Su-Ill,Na, Jee-Yeong,Jo, Do-Hyun,Lee, Chun-Yung 한국응용생명화학회 1987 한국농화학회지 Vol.30 No.1
To investigate ginseng oligopeptides with biological activities, the water extract was purified by ultra-filtration, gel filtration, ion-exchange and thin layer chromatography. Ultra-filtered water extract exhibited antilipolytic activity, inhibiting epinephrine-induced lipolysis in the isolated fat cells of rat epididymal adipose tissue. The filtrate was separated into 3 fractions by Sephadex G-25 gel filtration. Peptides were found only in the first fraction(S-FI). Saponine and sugars were also detected in tie fraction. S-FI fraction resolved further into 6 fractions by Dowex 50 ion-exchange chromatography. The sugar and saponine depleted fraction(P-F2) from the second chromatography showed antilipolytic activity. The P-F2 fraction revealed 6 spots on TLC. The 6 spots were isolated by TLC and identified as peptides. 인삼 성분중 생리적 활성을 가지고 있는 oligopeptide를 검출하고 분리하기 위하여 물 추출액을 ultra-filtration 한 후 gel filtration, ion-exchange chromatography 및 thin layer chromatography 등을 행하였다. 인삼 물 추출액으로부터 고분자 물질을 제거한 ultra-filtrate는 epinephrine에 의해 유도된 fat cell의 lipolysis를 저해하는 antilipolytic activity를 보유하고 있었으며 Sephadex G-25 gel filtration에 의해 3개 fraction으로 나뉘어졌다. 이중 첫번째 fraction(S-FI)만이 peptide 성분을 함유하고 있었으며 saponin 및 당도 검출되었다. S-FI fraction은 $Dowex\;50{\times}2\;ion-exchange\;chromatography$에 의하여 6개의 $fraction(P-F1{\sim}P-F6)$으로 분리되었고 TLC검정 결과 P-F2 fraction이 peptide fraction으로 antilipolytic를 보유하고 있었으며 TLC로 분리한 6개 spot는 각각 가수 분해한 전후의 TLC pattern을 비교해 본 결과 모두 oligopeptide임이 밝혀졌다. S-FI fraction에 존재한 saponine과 당은 P-F1 fraction 에서 모두 용출되었다.
정선옥 ( Sun-ok Jung ),이춘영 ( Chun-yeong Lee ),류회진 ( Hoe-jin Ryu ),최희진 ( Hee-jin Choi ),김지영 ( Ji-young Kim ),최채만 ( Chae-man Choi ),황인숙 ( In-sook Hwang ),신용승 ( Yong-seung Shin ) 한국산업보건학회 2023 한국산업보건학회지 Vol.33 No.1
Objectives: In this study, the safety of alcohol-based hand sanitizers (ABHSs) for quasi-drugs and cosmetics was investigated by analyzing the ethanol content, which is an active ingredient with a sterilizing effect, and methanol, which is toxic. Methods: Forty-one ABHSs were purchased at large supermarkets and online stores. Ethanol quantification was performed by gas chromatography-flame ionization detector, and methanol quantification was performed by headspace-gas chromatography-mass spectrometry. Results: The ethanol content of ABHS in quasi-drugs was 49.6-67.8%, which was suitable for standard manufacturing procedures for external disinfectants, and the ethanol content of ABHS in cosmetics was 9.1-61.3%. The methanol content of ABHS in quasi-drugs ranged from not detected(N.D.)-131.8 ppm, which was suitable for the methanol detection standard of ethanol raw materials in the Korean Pharmacopoeia. The methanol content of ABHS in cosmetics was 23.4-859.7 ppm, which was suitable for the detection limit of methanol in cosmetics. Conclusions: The ethanol and methanol content of ABHS was judged to be safe. When selecting an ABHS to be used for sterilization, it seems necessary to check the content of ethanol, an active ingredient, and use it according to its intended purpose.