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고려인삼(高麗人蔘)의 부위간(部位間) 화학성분(化學成分) 분포(分布) 및 상호관계(相互關係)
이종화,박훈,이정명,Lee, Chong-Hwa,Park, Hoon,Lee, Jung-Myung 한국응용생명화학회 1980 Applied Biological Chemistry (Appl Biol Chem) Vol.23 No.1
고려인삼(高麗人蔘)의 일반유기성분(一般有機成分) 및 saponin의 부위간(部位間) 분포(分布) 및 이들간(間)의 단순(單純) 상관(相關)에 대(對)하여 검토(檢討)하였다. 지하부(地下部)에서는 섬유(纖維), 회분(灰分), 지방(脂肪) 상호간고도(相互間高度)의 유의(有意) 정(正) 상관(相關)을 보이며 지상부(地上部)에서는 조지방(粗脂防)과 조단백질(組蛋白質)이 고도(高度) 유의(有意) 정(正) 상관(相關)을 보였으며 이들과 섬유(纖維)는 고도(高度)의 부상관(負相關)을 보였다. 전체(全體)에서 섬유(纖維)와 회분(灰分), 조지방(粗脂防)과 질소(窒素), Mn과 Cu, 회분(灰分)과 Ca, 회분(灰分)과 B는 정(正) 상관(相關)이고. 섬유(纖維) 와 단백질(蛋白質)과는 부(負) 상관(相關)이었다. 지하부(地下部)에서 회분(灰分) 지방(脂肪), 섬유(纖維)는 모든 무기양분(無機養分)과 고도(高度)의 정(正) 상관(相關)을 보이나 지상부(地上部)에서는 관계(關係)가 적고 관계(關係)가 있는 것도 부(負) 상관(相關)이 많다. 근부(根部)에서는 회분(灰分)과 Mg, K, Cu가, 지방(脂肪)과 B가, 섬유(纖維)와 Cu, K, Mg가 지상부(地上部)에서는 지방(脂肪)과 Mn이 특(特)히 상관(相關)이 높았다. 지상(地上), 지하(地下) 및 전체(全體)에서 유의상관(有意相關)을 보인 것은 회분(灰分)과 B, 지방(脂肪)과 Mn이었다. 지하부(地下部)에서 saponin은 N, P, Mg를 제외(除外)한모든 양분(養分) 및 섬유(纖維), 회분(灰分), 지방(脂肪)과 고도(高度) 정(正) 상관(相關)이 있으며 지상부(地上部)에서 K 및 섬유(纖維)와는 유의(有意) 부(負) 관계(關係), N, Mn, Cu, 지방(脂肪)과는 정상관(正相關)을 보였다. 지상(地上), 지하(地下) 전체(全體)에서 saponin과 유의(有意) 정(正) 상관(相關)을 보이는 것은 지방(脂肪), Mn, Cu이다. Crude fiber, ash and crude fat in various parts of root had highly significant correlation each other. In shoot, crude fiber showed highly significant negative correlation with crude fat or crude protein which were in highly significant positive correlation each other. In whole plant, crude fiber and ash, crude fat and crude protein, Mn and Cu, ash and Ca, and ash and B showed positive correlation respectively and negative between crude protein and crude fiber. Ash, crude fat and curde fiber showed highly significant positive correlation with all mineral nutrients in root while in shoot pairs haying significant correlation were less and most of these were in negative correlation. Ash with Mg, K or Cu, crude fat with B, crude fiber with Cu, K, Mg in root and crude fat with Mn in shoot were specially high significant pairs. Ash with B and crude fat with Mn were significant in root, shoot and whole plant. Saponin contents in root showed highly significant positive correlation with crude fiber, crude fat, ash and with all other mineral nutrients except N, P, Mg while, in shoot, it showed, negative correlation with K and crude fiber, ana positive with N, Mn, Cu, and crude fat. Saponin showed positive correlation with crude fat, Mn, Cu, in root, shoot and whole plant.
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이종화,김홍진,배효원,Lee C. H.,Kim H. J.,Bae H. W. 한국응용곤충학회 1978 한국식물보호학회지 Vol.17 No.3
가. 인삼의 모잘록병을 방제하기 위하여 10종의 약제를 공시하여 실내 및 포장시험을 실시하였다. 나. 공시약제의 효과를 조사하기 위하여 실내에서 토양관법으로 그리고 포장에서는 모잘록병균을 인위적으로 접종한 균포와 자연발병상태의 균포에서 종자소독, 토양소독, 토양관주법으로 처리하였던 바, 다. 모잘록병 방제를 위해 기대되는 약제로는 Homai, Captan, Validamycin, Tachigaren등이 있다. 1. The trials were planned to test ten different fungicides for the control of ginseng damping off Caused by Rhizoctonia solani in the laboratory and in the field. 2. Fungicidal activity was tested by soil drenching in test tube in the laboratory and with seedlings in t he seedbed naturally and artificially infested with damping-off organisms. 3. Promising chemicals to control damping-off of ginseng without marked phytotoxicity were Homai, Captan, Validamycin and Tachigaren.
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토복령알코홀추출물이 토끼 적출 십이지장관운동에 미치는 영향
이종화,김정일,송민자,Lee, Jong-Hwa,Kim, Chung-Il,Song, Min-Ja 대한약리학회 1973 대한약리학잡지 Vol.9 No.2
Chinae Rhizoma is dried crude roots and rhizomes obtained from Smilax china Linne which belongs to Smilax Genus of Liliaceae Familia, and it has been in wife use as a folk remedy for a long time. But very little systemic studies on its pharmacological actions have been made until now. Furthermore, the effective components except saponin of Chinae Rhizoma have been not known yet. It came to the attention of the authors that Chinae Rhizoma are used as antidiarrhetics in Chinese herb medicine, therefore they attempted to observe the effects of Chinae Rhizoma Alcoholic Extracts (Chinae Rhizoma Ethanol Extract=CREE, Chinae Rhizoma Methanol Extract=CRME) from Smilax china Linne on motility of the isolated rabbit duodenum in this experiment. The results of the study were as follow: 1. The movement of the isolated rabbit duodenum was slowed down and its tonus was relaxed in proportion to the concentration of Chinae Rhizoma Alcoholic Extracts (CREE & CRME). 2. With a concentration of CREE $10^{-4}g/ml$, the contractile effects of acetylcholine $10^{-6}g/ml$, pilocarpine $10^{-5}g/ml$ and physostigmine $10^{-5}g/ml$ were remarkably blocked in the movement of isolated rabbit duodenum. 3. The concentration of the isolated rabbit duodenum induced by barium chloride $10^{-4}g/ml$ was significantly inhibited by CREE $10^{-4}g/ml$. 4. With the administrations of histamine $10^{-5}g/ml$, serotonin $10^{-6}g/ml$ and prostaglandin $A_2\;10^{-6}g/ml$ respectively, contractions were observed in the rabbit duodenum. But, treatment of CREE $10^{-4}g/ml$ inhibited markedly the tonus and motility of the duodenum pretreated with the above drugs. 5. The effects of CREE and CRME were very much alike in this experiment. From the above results, it is concluded that the mechanism of action of Chinae Rhizoma on motility of isolated rabbit duodenum was partially due to cholinergic blocking effect.
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마산, 진해 연안해역 해저퇴적물중의 중금속류 함량에 관한 연구
이종화,한상준,윤웅구,Lee, Jong Wha,Han, Sang Joon,Youn, Oong Koo 한국해양학회 1974 韓國海洋學會誌 Vol.9 No.1
Concentration of heavy metals in the sediments from the sea off Jinhae and Masan have been studied. Concentration of the elements varied among the stations as the following: 0.32-0.76% Fe, 0.01-0.06% Mn, 29-120ppm Zn, 1-16ppm Co, 2-38 ppm Ni, 2.5-12.4ppm Pb and 0.2-0.7ppm Cd. General tendency of the concentraion of elements except Mn showed considerably high values at stations in the inner bay. This tendency seemed to be caused by waste water from industrial activities in the adjacent land. It can be thought that the pollution status of the studied area is still limited in the inner part of the day.
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Calcium Movement in Carbachol-stimulated Cell-line
이종화,Lee, Jong-Hwa The Korean Society of Pharmacology 1995 대한약리학잡지 Vol.31 No.3
Calcium수송에 대한 기전을 추구하기위하여, carbachol을 사용하여 ml muscarinic receptor-transfected RBL-2H3 cell-line에서 다음과 같은 실험결과를 얻었기에 이에 보고한다. 1) Carbachol의 투여로 이들 cell-line에서 $Ca^{2+}$ influx가 농도에 따라 증가하였고, hexosaminidase 분비양도 의의있게 증가하였다. 2) Atropine 투여로 Carbachol의 상승작용이 의의있게 억제되었다. 3) 수종의 금속양이온을 투여하여 carbachol의 $Ca^{2+}$수송에 대한 영향을 관찰한 바, 이들 금속이온들은 $Ca^{2+}$의 influx를 의의있게 억제하였다. 4) PMA(20 nM) 투여로 carbachol의 hexosaminidase의 분비는 억제되지 못했지만 $Ca^{2+}$ influx는 억제되었다. 5) PTx $(0.2\;{\mu}g/ml)$ 투여로 carbachol의 hexosaminidase 분비가 의의있게 억제되었다. 위의 결과로 미루어 보아, 이 세포의 muscarinic receptor가 calcium channel을 통한 calcium수송에 매우 중요한 영향을 나타내는데, 이들 calcium ion channel은 적어도 두 종류가 존재하며, 하나는G-protein-dependent calcium channel에 의하며, 다른 하나는 G-protein-independent calcium channel에 대한 작용에 의한 것으로 생각된다. 또한 이 calcium channel들은 2가 또는 3가의 다른 금속 ion들에 의하여 calcium수송이 억제된다. It has been well known that the intracellular calcium concentration $([Ca^{2+}]_i)$ in living cell is very sensitive to live or to survive, but the transmembrane system of calcium ion, especially mechanism of calcium ion movement in unexcitable state has been little elucidated. Though many proposed theories for calcium ion transport have been reported, it is still unclear that how could the sustained maintenance in cytosolic calcium level be done in cell. Since one of possible mechanisms of calcium transport may be related to the acetylcholine receptor-linked calcium channel, author performed experiment to elucidate this mechanism of calcium influx related to cholinergic receptor in ml muscarinic receptor-transfected RBL-2H3 cell-line. 1) The effects of carbachol both on calcium ion influx and on the secretion of hexosaminidase were respectively observed in the manner of time-related or concentration-dependent pattern in this model. 2) The effects of several metal cations on calcium transport were shown in carbachol-induced cell-line. 3) Atropine was administered to examine the relationship between cholinergic receptor and calcium ion influx in this model. 4) PMA (Phorbol 12-myristate 13-acetate) or PTx (Pertussis toxin) was respectively administered to examine the secondary mediator which involved pathway of calcium ion movement in carbachol-induced cell-line. The results of this experiments were as follows; 1) Carbachol significantly stimulated both the calcium influx and the secretion of hexosaminidase in the manner of the concentration-dependent pattern. 2) Atropine potently blocked the effects of carbachol in concentration-response manner. 3) Administered metal cations inhibited the calcium influx in carbachol-stimulated this model to the concentration-related pattern. 4) PMA did not inhibit carbachol-induced secretion of hexosaminidase, but blocked the calcium influx in this cell-line. 5) The suppression of carbachol-induced hexosaminidase secretion was shown in PTx-treated cell -line.
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이종화,봉종헌,한상준,Lee, Jong Wha,Bong, Jong-Han,Han, Sang Joon 한국해양학회 1974 韓國海洋學會誌 Vol.9 No.1
Current observations were carried out in Jinhae Bay area during the period of January to February 1974. The data were synthetically analyzed and the characteristics of the water movement in Jinhae Bay were studied. The water movement in Jinhae Bay area is a reversing tidal current type and the Semi- diurnal tidal current is predominant. The ebb current begins at about high water time and the strongest current occurs at about 3 hour after high water. The flood current begins at 0.2-1.0 hour before low water and the strongest current occurs at about 3-4 hour after low water. The main ebb current flows to SE direction with the maximum welocity, about 100cm/sec and the lood flows to NW with the maximum velocity, about 70cm/sec. Generally, the ebb current in Jinhae Bay is more predominat than the flood current except at the west and the north coast area of Gadeog-do where the flood current is more predominant.
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