침전극저주파 자극이 해마의 세포사 차단에 미치는 영향

이정숙 ( Jung Sook Lee ),김병기 ( Byung Ki Kim ),김성원 ( Sung Won Kim ),김지성 ( Ji Sung Kim ),김동일 ( Dong Il Kim ),송치원 ( Chi Won Song ) 대한한방신경정신과학회 2009 동의신경정신과학회지 Vol.20 No.2

Objectives: This study was performed to investigate the effects of Needle Electrode Electrical Stimulation (NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of ** arteries in white mice, the amounts of necrosis and inflammation related substances IL-6, Caspase-3, and PARP, C-fos were measured in neurons of the hippocampus. The following results were obtained. Methods: This study used 21 male specific pathogen free (SPF) SD (Sprague Dawley) rats, 8 weeks of age and approximately 300 g in weight, that were given at least 1 week to adapt to the lab environment Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group for comparison with the common carotid artery occlusion models, a GI group that underwent common carotid artery occlusion, and a needle electrode electrical stimulation (NEES) group that underwent NEES after artery occlusion. The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device (PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the right and left acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immunohistochemistry test was done on the forebrains of the GI induced rats. All the data collected from this study was symbolized and analyzed using a statistics processing program (SPSS 12.0K/PC). The level of significance was set at α=0.05 and a T-TEST analysis was used to find out the effects of treatment on each of the groups: the normal group, the CVA induced group, and the treatment after CVA induction group. Results: Both PARP and C-fos immuno-reactive cells, related to apoptosis, were greater in the GI groups than the NEES group. Caspase and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. Conclusions: This research was conducted to study the effects of NEES on CVA due to ischemia. Occlusion and reperfusion was performed on the common carotid arteries of white rats, after which amounts of substances related to neuron necrosis and inflammation-PARP, IL-6, Caspase-3, and C-fos - were measured in the Hippocampus.

운전재활프로그램이 노인의 인지기능에 미치는 효과

이정숙 ( Sung Sook Lee ),김보라 ( Bo Ra Kim ),하재영 ( Jae Young Ha ),박지민 ( Ji Min Park ),조예슬 ( Ye Seul Cho ),하진리 ( Jin Ri Ha ),홍우선 ( U Seon Hong ),김성원 ( Sung Won Kim ) 대한통합의학회 2014 대한통합의학회지 Vol.2 No.4

Purpose : The purpose of this experiment is to find out the effectiveness which exert influence on cognitive skills by using the Driver Rehabilitation program for senior citizens who are over 65 years old and live in Busan. Method : From July first,2014 to August 28th, 2014, we researched the 60 elderly people who are over 65 and go to community relief center which is in Busan. - 30 are experimental group and the other is control group. In the experimental group, we used Nintendo wii`s driving simulation program and RC Car driving program in the model road. For estimation, we used MVPT-3(Motor-Free Visual Perception Test-3), Trail Making Test - 1, Trail making Test - 2 and LOTCA(Loewenstein Occupational Therapy Cognitive Assessment). Result : Nintendo wii`s driving simulation program and RC Car driving program in model road results efficient visual perception ability. This programs results effectively in visual perception ability and space perception ability. This programs results effectively in motor apraxia ability. This programs results effectively in control ability for visual perception. This programs results effectively in thinking operation. Conclusion : Nintendo Wii`s driving simulation program and RC Car driving program in model road positively influence improving for visual perceptual ability and cognitive function of elderly people. Also it is considered as being more efficient for improving visual perceptual ability and cognitive function to implement basic rehabilitation training with driving rehabilitation program than basic training itself.

Orbit Determination for the KOMPSAT-1 Using GPS Navigation Solutions

Byoung-Sun Lee(이병선),Jeong-Sook Lee(이정숙),Ho-Jin Lee(이호진),Sung-Pal Lee(이성팔),Jong-Ah Kim(김정아),Hae-Jin Choi(최해진) 한국항공우주학회 2000 한국항공우주학회 학술발표회 논문집 Vol.- No.-

유아기 신체 건강과 관련한 일상생활습관 실태 연구

이정숙(Jung Sook Lee),김성재(Sung Jae Kim) 한국아동학회 2009 아동학회지 Vol.30 No.3

상황버섯 및 카레를 첨가한 잡곡밥 추출물의 LLC-PK1 세포에서의 산화적 스트레스 보호 효과

이정숙(Jung-Sook Lee),송가락(Jia-Le Song),길정하(Jeung-Ha-Kil),정병진(Byung-Jin Jeong),정종성(Jong-Sung Jeong),허태곤(Tae-Gon Huh),박건영(Kun-Young Park) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.11

잡곡밥 메탄올 추출물이 가지는 산화적 스트레스 개선 효과를 확인하기 위하여 H₂O₂로 유도된 산화적 스트레스에 대한 LLC-PK1 세포의 보호 효과를 조사하였다. 250 μM의 H₂O₂에 의한 산화적 스트레스가 유발된 LLC-PK1 세포에 시료 추출물을 처리한 결과, WR, TMR, GR 순으로 H2O2 처리군에 비해 세포 생존율이 증가하였다(P<0.05). 특히 GRS와 GRK에서 정상군에 가까운 세포 생존율을 나타내어 H₂O₂에 의한 산화적 스트레스로 유발된 세포 손상에 대한 잡곡밥 추출물의 보호 효과를 확인할 수 있었다. 세포 내 활성산소(ROS)의 수준과 세포 내 지질과산화물질(MDA)의 생성 억제 효과 역시 WR, TMR, GR, GRK, GRS 순으로 증가하는 것을 관찰하였다. H₂O₂로 인하여 세포 내 항산화 효소인 SOD, CAT와 GSH-px 등의 활성이 감소된 LLC-PK1 세포에 잡곡밥 추출물을 처리했을 때 이들 효소의 활성이 증가했으며 특히 GRK, GRS군에서 현저하게 증가되었다. LLCPK1 세포에서 H₂O₂에 의해 발생하는 산화적 스트레스에 대한 보호 효과를 측정한 결과 잡곡밥 추출물은 세포 손상을 억제하고 세포 내 활성산소(ROS)의 수준과 지질과산화물질(MDA)의 생성을 억제하며, 세포 내 항산화 효소의 활성을 증가시키는 효과를 가지는 것으로 보인다. 또한 항산화 효소 유전자 발현에서도 위와 비슷한 효과를 나타내었다. 이상의 결과로 잡곡밥 메탄올 추출물, 특히 기능성 물질인 카레가루와 상황버섯 추출물이 첨가된 잡곡밥은 LLC-PK1 세포에 대한 보호 작용과 in vitro에서의 항산화 효과가 높은 것으로 확인되었다. The aim of this study was to investigate the protective effects of methanolic extracts of cooked mixed grain rice samples, including grain rice (sorghum, black bean, proso millet, and Job"s tears) mixed with fermented brown rice (GR), GR added with 0.5% water extract of Sanghwang mushroom (GRS) or 0.1% curry (GRK), and traditional five grain mixed rice (TMR, Ohgokbap), on H₂O₂-induced oxidative injury in LLC-PK1 pig renal epithelial cells. White rice (WR) was used as a positive control. Cells were first exposed to H₂O₂ (250 μM) for 4 hr, followed by treatment with 100 μg/mL of different GR extracts for 24 hr. H₂O₂ significantly induced cell damage (P<0.05). Cellular levels of reactive oxygen species (ROS), lipid peroxidation, and antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-px), were measured. In addition, mRNA levels of antioxidant enzymes were determined by RT-PCR assay. Mixed grain rice, particularly GRS and GRK, were able to reduce cellular levels of ROS, decrease lipid peroxidation, and also increase mRNA expression of antioxidant enzymes compared to other samples. These results suggest that mixed grain rice, specifically GRS and GRK, have strong protective effects against H₂O₂-induced oxidative injury in LLC-PK1 cells through inhibition of lipid peroxidation, reduction of ROS levels, and elevation of antioxidant enzyme activities.

노숙자 자아분화와 부적응 증상연구

이정숙(Lee Jung Sook),김성천(Kim Sung Chun) 한국가족치료학회 2001 가족과 가족치료 Vol.9 No.2

본 연구는 노숙자들의 불우한 성장환경을 고려하여 노숙자의 자아분화수준과 부적응 증상과의 관계를 파악하는데 그 목적이 있다. 본 연구는 292명의 노숙자를 대상으로 하였으며 연구결과는 다음과 같다. 첫째, 노숙자들이 똑같은 사회적 경제적 상실로 인해 위기감을 맞고 노숙생활을 하고 있지만, 이들의 이전직업이 무직이고 교육수준이 낮으며 자라온 가정환경이 열악했음을 알 수 있었다. 또한 노숙 후에 적응이나 정신적 스트레스 및 심리적 소외감, 갈등의 정도가 높은 것으로 나타났다. 둘째, 노숙자들이 노숙을 하게 되는 경우가 원가족 해체의 경험이나 가족과의 위기상황에서 적절한 대처능력을 찾지 못하기 때문인 것으로 확인되었다. 셋째, 자아분화수준이 낮을수록 부적응 증상의 하위 영역에서 역기능적인 특성이 높게 나타났다. 마지막으로 부적응 증상에 가장 많은 영향을 주는 요인은 자아분화이며, 또한 부적응 증상에 영향을 주는 변인으로 결혼상태에서 미혼 및 이혼, 별거, 사망인 경우가 오히려 부적응 증상이 낮게 나타났다. 연령이 높을수록, 이전직업이 사무직 이상일수록 부적응 증상이 높게 나타났다. 노숙자들 중 결혼상태에서 가족원을 두고 나온 경우 심리적 고통이 더 강한 것을 알 수 있으며 노숙이전의 직업 및 연령에 따라 노숙에 대한 적옹정도가 다름을 알 수 있었다 This study was about 292 of the homeless people self-differentiation and their inadequate symptoms of mental health by the theoretical factors which was in the social economic status. Also it was showed the correlation between the self-differentiation and inadequate symptoms of mental health. According to the main results of chis study, there was difference in the relationship among their family members and home environment by their educational level and previous occupation. And it was showed that they were in the state of mental stress and inadequacy. They felt alienated sense after they had lived without home. And when the level of self-differentiation that was explained their negative sense about the relationship among family members in their home environment of childhood was low, they showed that they were in the highest level of the inadequate symptoms.

일과성 전뇌허혈 유발 쥐의 침전극 저주파자극 후 대뇌의 c-Fos 발현에 미치는 영향

이정숙(Jung Sook Lee),김성원(Sung Won Kim) 한국전문물리치료학회 2010 한국전문물리치료학회지 Vol.17 No.1

Ischemia that causes strike induces inflammation of brain cells and apoptosis and as a result. it influences much in the functional part of a man. The needle electrode electrical stimulation (NEES) that combines acupuncture of oriental medicine with electric therapy of western medicine relieves inflammation of cells and has effect on regrowth of nerve tissues. This study was conducted to verify the influence of MEES on the occurrence of c-Fos of cerebrum after applying NEES to the meridian point, Zusanli (ST36) of rats with induced ischemia. Global ischemia was induced by using ligation method on common carotid artery of male Sprague Dawley (SD) rats. The ligation was maintained for 5 minutes and then suture was removed for blood reperfusion. After inducing global ischemia, NEES was done to the left and right meridian points of Joksamri of a rat for 30 minutes after 12 hours, 24 hours, and 48 hours. The findings were as follows. 1. In the result of immunohistochemical method, the number of c-Fos immune response cells significantly decreased(p<.05) in NEES group than the control group (G1) that did not get NEES. 2. In the result of western blotting, the occurrence of c-Fos after 24 hours from the inducement of ischemia significantly decreased (p<.05) in NEES group than the control group (G1) that did not get NEES. Therefore, as the effect of NEES was shown highest after 24 hours from the ischemia, it is suspected that NEES would take important role in early treatment after cerebral stroke.

朝鮮中期 出土 綿織物의 理化學的 特性

李貞淑(Jeong Sook Lee),金聲連(Sung Reon Kim) 한국염색가공학회 1996 韓國染色加工學會誌 Vol.8 No.3

교육용 앱 평가도구 개발 연구

이정숙(Jeong-Sook Lee),김성완(Sung-Wan Kim) 한국컴퓨터정보학회 2013 한국컴퓨터정보학회 학술발표논문집 Vol.21 No.1

이 연구는 스마트교육환경에서의 교육용 앱을 평가하기 위한 신뢰롭고 타당한 도구를 개발하는데 있다. 기존 선행연구에 기초해서 교육용 앱의 평가를 위한 평가모형을 도출했으며, 이 모형은 4개의 평가영역(교수 학습측면, 화면디자인측면, 기술측면, 경제 윤리측면)과 13개 평가요소들로 구성되었다. 이 잠재모형의 통계적 타당성 검증을 위한 자료수집을 하고자, 경기도 소재 중학교 1곳과 고등학교 2곳의 학생 156명을 대상으로 교육용 앱을 평가하는데 있어서 각 평가문항이 갖는 중요도를 평가하였다. 수집된 자료를 탐색적 요인분석한 결과, 교육용 앱을 평가하는 영역으로 교수 학습(흥미성 자기주도성 실용성, 인지발달성), 화면디자인(디자인의 적합성, 어휘의 정확성), 기술(호환성, 안정성), 경제 윤리(경제성, 윤리성) 등 4개 영역이 제안되었다. 또한 문항내적일관성을 확인하고자 신뢰도 분석한 결과, 각 평가영역 별 Cronbach α는 .88, .85, .82, .80으로 모두 적합한 수준을 보였다. 따라서, 이 연구를 통해 도출된 교육용 앱 평가도구는 통계적으로나 타당성과 신뢰성 측면에서 의미 있는 것으로 판단할 수 있다.

행복교육을 위한 미술교육 방안

김성숙 ( Sung Sook Kim ),이정숙 ( Jung Suk Lee ) 한국예술교육학회 2015 예술교육연구 Vol.13 No.1

이 연구는 학교폭력을 완화하고 어린이의 ‘꿈과 끼’를 키워주는 교육의 한 방법으로 ‘행복교육’ 을 위한 미술교육 방안을 도출하여 제시하고자 한다. 연구 방법은 현장교사와 공동으로 수업적용을 통한 실험연구를 하였다. 연구내용은 1. 행복교육의 실천을 위한 미술교육활동의 단계를 나-너(타인)-우리(주위)중심의 3단계로 설정하고, 2. 각 단계별로 다양함의 수용과 상호 소통능력을 증진하는 미술교육을 실천하였다. 그리고, 3. 수업적용 결과 분석을 바탕으로 행복교육을 위한 미술교육 방안을 도출하여 제시하고 향후 미술수업의 방향을 제언하였다. 행복교육을 위한 미술교육은 첫째, 학습자의 내면의 교육에 보다 초점을 맞추어야 한다. 둘째, ‘교사는 미술교육을 통해 세상을 바꾸는 자’라는 새로운 인식을 가져야 한다. 셋째, 일원론적-관계적 사고에 기초한 미술교육을 확대해야 한다. 금후, 초등현장에서 행복교육을 위한 미술수업에 대한 탐색과 연구가 절실히 필요하다. 행복한 미술교육은 창의성과 인성, 융합적 사고를 갖춘 인재의 육성은 물론이고, 보다 행복하고 좋은 세상으로 변화시키는 힘으로 작용하기 때문이다. This study suggests art education as a means to realize ‘happiness education’ to develop children’s dreams and talents and to alleviate school violence. This study is experimental research applied in a classroom by a field teather. With respect to the content of the research, stages of art education activities are established here for the practice of happy education. This involves three stages, focusing on I, you(the other), and we(those surrounding us) relationship and secondly on conducting art education to promote the acceptance of diversity and mutual communicative abilities at each stage. After conducting phased classes with the themes of ‘Decorating my own character’, ‘Decorating my friends’ character’ and ‘Creating an appreciative card’, this study then discusses the following three outcomes by producing an art education plan for ‘happiness education’ on the basis of educational effect, which is the result of the application to the class. The suggestions of art education for ``happiness education`` are as follows. First, it is necessary to focus more on the education of learners’ inner sides. Second, educators need to change their recognition of a ‘Teacher as a person who changes the world through art education’. Third, it is necessary to expand art education based on monistic-relational thinking. Even now, inquiring into and conducting research on art classes for happiness education are urgently needed in the elementary educational setting, as happy art education has the power not only to cultivate talent with creativity, personality and integrated thinking, but also to inspire change to lead to a happier and better world.