유전자 형질전환을 통한 쌀 전분 분해효소 재조합 효모균주의 개발과 발효특성조사

이자연,진종언,배석,Lee, Ja-Yeon,Chin, Jong-Eon,Bai, Suk 한국미생물학회 2016 미생물학회지 Vol.52 No.2

쌀 전분을 원료로 효모를 직접 이용하여 항산화제 glutathione(GSH)이 풍부한 알코올 음료를 제조할 목적으로 GSH 함량과 당화능을 증진시키기 위하여 Saccharomyces cerevisiae의 ${\gamma}$-glutamylcysteine synthetase 유전자(GSH1), Debaryomyces occidentalis의 glucoamylase 유전자(GAM1), 그리고 ${\alpha}$-amylase 유전자(AMY)를 청주 효모 S. cerevisiae에서 공동 발현시켰다. 재조합 청주 효모의 세포외 GSH 함량은 원균주에 비해 1.5배 증가하였다. 2% (w/v) 쌀 전분이 함유된 배지에서 배양하였을 때 GAM1과 AMY 유전자 모두 발현하는 효모 균주의 glucoamylase에 의한 당화능은 GAM1유전자만 발현하는 균주와 비교하여 2배 증가하였다. 이 새로운 균주는 쌀 전분이 20%(w/v) 함유된 배지에서 7일간 발효를 통해 에탄올 11% (v/v)를 생산하였고, 전분 함유량의 90% 이상을 소비하였다. To improve antioxidant glutathione (GSH) content and saccharification ability in sake yeasts of Saccharomyces cerevisiae, the ${\gamma}$-glutamylcysteine synthetase gene (GSH1) from S. cerevisiae, glucoamylase gene (GAM1) and ${\alpha}$-amylase gene (AMY) from Debaryomyces occidentalis were co-expressed in sake yeasts for manufacturing a refreshing alcoholic beverage abundant in GSH from rice starch. The extracellular GSH content of the recombinant sake yeasts increased 1.5-fold relative to the parental wide-type strain. The saccharification ability by glucoamylase of the new yeast strain expressing both GAM1 and AMY genes was 2-fold higher than that of the yeast strain expressing only GAM1 gene when grown in the culture medium containing 2% (w/v) rice starch. It generated 11% (v/v) ethanol from 20% (w/v) rice starch and consumed up to 90% of the starch content after 7 days of fermentation.

2/2 사문조직물의 이축신장거동

이자연,이덕래,임진모,Lee, Ja-Yeon,Lee, Deok-Rae,Im, Jin-Mo 한국섬유공학회 1984 한국섬유공학회지 Vol.21 No.4

In order to investigate the biaxial load-extension behavior of 2/2 twill fabrics, a mechanical model for biaxial extension was suggested. The suggested model was analysed by Castigliano's theorem and graphical method was adopted for the solution of the equations. The solutions were compared with experimentals.

초기 불교적 세계관에 비춰 유기체적 생태학적 세계관에 입각한 새로운 패러다임 탐구하기: 불교 위빠사나 명상을 특별히 참고하여

이자연 ( Ja-yeon Lee ) 한국정치평론학회 2016 정치와 평론 Vol.18 No.-

It is said that it is urgently needed to design a new paradigm in order to solve various problems facing us in contemporary times. The purport of this essay is to investigate the possibility of designing a new paradigm which is based on an organic, ecological world view by considering this problem in the light of the Buddhist world view. The reason why the Buddhist world view is taken into account is that it is free from the chronic problem of objective approaches as in Western disciplinary traditions, namely conceptual construction. In that attempt the non-dual character of the Buddhist world view that imply unity in dual correlation between the material object and the mind is brought out to light. In this essay vital energy is stressed as a key concept of the holistic, ecological world view. Buddhist meditation called Vipassana is specially referred to in order to bring the divine level of vital energy, namely spirituality relevant to our discussion. For spirituality is what makes human beings differ from other beings on the earth. Another reason for dealing with Buddhist meditation lies in my idea that we need insights into ecosystems in order to design a new paradigm based on the organic, ecological world view and it is an appropriate method for that purpose.

생태계와 조화를 이루는 새로운 경제 패러다임에 대한 철학적 탐구

이자연 ( Ja Yeon Lee ) 한국정치평론학회 2014 정치와 평론 Vol.15 No.-

The present economic crisis and the environmental crisis can be analyzed as having the same cause, namely, the excessive industrialization which entailed unbalanced value system coupled with the prevailing capitalism and the problem of inequity. Now that we consider the value of justice in connection with economics, in the long run it turns around the matter of designing a new paradigm for economy in future to see to it that every field of human society is incorporated into a unified network as in ecosystem. The purpose of this paper is to deal with various problems coming in the way to design such a new paradigm and to find a possible way for them to converge to eco-social sustainability. The relevance of the matter of designing a new paradigm for eco-social sustainability to the issue of justice in relation to economics consists in my view that the ultimate source of the universal justice is the law of nature.

일본 헤이안 말·가마쿠라시대 공가의 복식과 문화

이자연 ( Ja Yeon Lee ) 한국의류산업학회 2012 한국의류산업학회지 Vol.14 No.2

This study, focusing on the costumes of Kouge in the era of Kamakura and the end of Heian in Japan, aims to explore the aspects and characteristics of the costumes that were developed under political, economical, and social circumstances. The findings are the following. Male costumes include Sokdae, Pogo, Euigwan, Jikeui, Sueui, and Sugan, while female costumes include Shipidan, Sogyu, Sejang, and Hansam. Different clothes of Kuge were chosen depending on the types and purposes of the occasions: full dress and Yahkjang, a semi-dress, for a ceremonial purpose, street costume for a personal visit purpose, outdoor clothing for hunting and outdoor activities, Cheongjangsok, and Sukjikjangsok. In these various types of clothes we can assume the elegant life of aristocratic class of Kouge. In addition, different fabrics, patterns, and dresses were chosen depending on the grade of ranks of Kouge, so that Kouge can differentiate their ranks, keep the order of the ranks, and enhance their authority by themselves. With the advent in the era of Kamakura, the power of Kouge was weakened and their clothes underwent changes, some types of clothes were omitted or simplified or the clothes of subordinates were worn. In addition, when new forces appeared and new clothes came about, there were changes in the purpose and the wearer of the clothes: from ordinary clothes to official clothes, from the clothes of lower class to the clothes of higher class, and from Sukjikjangsok to Cheongjangsok.

유도된 상호작용성을 통한 가상환경에서의 현실감 제고

이자연 ( Ja Yeon Lee ),김정현 ( Joung Hyun Kim ) 한국감성과학회 2003 춘계학술대회 Vol.2003 No.-

가상환경에서의 실재감을 증대시키는 방법 중에 하나는 상호작용을 이용하는 방법이다. 즉, 사용자가 가상환경에 변화를 줄 수 있게 하여, 사용자와 가상환경 사이의 결속감을 통해, 사용자가 가상환경 속에 있는 듯한 느낌을 주는 것이다. 그러나, Architectural Walkthrough 혹은 Navigation 시스템은 기능적으로 가상객체와에 affordance 와 그 밖의 interactivity cue를 적용하여 사용자로 하여금 시각적으로만 상호작용감을 유발하게 하여, 사용자의 실재감을 높이기 위한 방법에 대한 것이다. 두 개의 가상환경에 대하여 아무런 visual cue가 없는 경우, 텍스트를 이용한 직접 표현 방법, 객체가 simulation 된 trace 그리고 affordance cue 등 총 4가지 방법들, 즉 총 8가지 실험 조합에서 사용자가 느끼는 상호작용성과 실재감을 측정하였는데 interactivity유도를 위한 간접적인 visual cue가 쓰였을 경우 통계적으로 유의하게 상호작용성 및 실재감이 증대하는 결과를 보여주었다. 이는 적은 모델링만의 노력으로, 실제 인터액션에 대한 구현 없이 가상환경에서 어느 정도의 실재감을 높일 수 있는 방법을 제시하고 있다.

자유한국당의 운명

이자연 ( Ja-yeon Lee ) 한국정치평론학회 2018 정치와 평론 Vol.23 No.-

[P3-220610] 반려식물 프로그램이 독거노인의 주관적 안녕감 및 우울감에 미치는 영향

이자연(Ja-Yeon Lee),박시정(Si-Jeong Park),안효진(Hyo-Jin An),제의숙(Eu-sook Jea) 인간식물환경학회 2022 인간식물환경학회 학술대회 Vol.2022 No.춘계

조선전기 조일간의 교역품을 통하여 본 복식문화(Ⅱ)

이자연(Lee Ja Yeon) 한국복식학회 2003 服飾 Vol.53 No.7

This study is to research the changing trends in the trading goods and the cause of the change in the early Chosun dynasty and to find out the influence that the export goods had on the Chosun society. This research demonstrated the costume culture of the early Chosun was affected by the trading trends. The export items of Chosun showed differences in chronological order. They changed from hemp cloth to cotton cloth. The cause of such change in the export items was due to the change in the amount of demand and supply. to products of Chosun. and to social factors. Looking at the amount and items of the export goods to Japan, the amount was huge and the number of trade was a lot. There were several influences that the exporting cotton cloth to Japan had on Chosun`s costume culture. First, the export caused the growth of cotton industry through the reinforcement policy. Next. it made the amount of national deposit of cotton cloth exhausted as a result of the increase in the amount of the exporting cotton. It also made worse the dual distribution structure of cotton cloth and the leaning toward bad cotton cloth. And in consequence of the connection between rich merchants and politicians, these social phenomena became worse and worse. And these facts demonstrate that the costume culture of the early Chosun dynasty was affected by the trade between Chosun and Japan. Therefore, to better understand the costume culture of the early Chosun dynasty, I propose to consider the consequences resulted from the trade with Japan.

일본 고분시대의 하니와 ( 식윤 (埴輪) ) 의 복식에 관한 연구

이자연(Ja Yeon Lee) 복식문화학회 1999 服飾文化硏究 Vol.7 No.5