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이원흥,왕희정,소의영,손우영,김욱환,김명욱 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1
Changes in arterial ketone body ratio(AKBR) during and after liver surgery were investigated in 11 patients who underwent partial hepatectomy or orthotopic liver transplantation during the period from April 1995 through June 1995. During partial hepatectomy, hepatic vascular occlusion induced reduction in AKBR to below 0.4 in 10 cases, indicating that AKBR rapidly reflects the intraoperative metabolic insults imposed on the liver. After the completion of operation, AKBR's returned to the normal value of above 0.7 in cases without cirrhosis. In contrast, 4 of 5 cases with cirrhosis exhibited delayed recovery of AKBR(p=0.053). The mean value of ICG R15 in the delayed recovery group of serial AKBR was 14.7%, significantly lower than 8.0% in the rapid recovery group(p=0.031). It seems that postoperative restoration of liver function in patients with poor hepatic functional reserve takes more time, as reflected in delayed recovery of AKBR. Changes in AKBR during and after liver transplantation were as follows: reduction to below 0.4 during anhepatic phase; recovery to above 0.4 concomitant with functioning of the new liver; maintenance at over 0.7 during the first week after the operation. Such findings were useful in monitoring the function of the grafted liver during the critical perioperative period.
Cellobiose와 xylose를 동시에 대사하는 Pichia stipitis 돌연변이를 활용한 동시당화발효
김대환,이원흥 한국생물공학회 2019 KSBB Journal Vol.34 No.4
In order to economically produce biofuels in simultaneous saccharification and fermentation (SSF) of cellulosic biomass, it is required to construct microbial host efficiently utilizing various sugars. Previously, a mutant Pichia stipitis YN14 strain co-fermenting cellobiose and xylose was isolated. In this study, ethanol production performance of P. stipitis YN14 was compared with the parental P. stipitis CBS 6054 through the series of SSF experiments. In SSF of cellulose, the mutant strain employing Celluclast 1.5 L as the cellulolytic enzyme produced 6.1 g/L of ethanol, which was 5.5- times and 1.2-times higher than the parental strain with Celluclast 1.5 L and Cellic CTec2, respectively. In addition, the mutant YN14 strain with Celluclast 1.5 L showed 26.6 g/L of ethanol production in simultaneous saccharification and cofermentation (SSCF) of cellulose and xylose, which was 75% and 60% higher than the parental CBS 6054 strain with Celluclast 1.5 L and Cellic CTec2, respectively. Our results propose that efficient ethanol production from the cellulosic biomass would be accomplished by utilization of yeast strain fermenting cellobiose and xylose simultaneously.
적응진화를 활용한 cellobiose와 xylose 동시발효 Pichia stipitis의 개발
김대환,이원흥 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4
Production of biofuels and value-added materials from cellulosic biomass requires the development of a microbial strain capable of efficiently fermenting mixed sugars. In this study, the natural xylose fermenting yeast, Pichia stipitis, was evolved to simultaneously ferment cellobiose and xylose. Serial subcultures of wild-type P. stipitis in 20 g/l cellobiose were performed to increase the rate of cellobiose consumption. A total of ten rounds of the serial subculture led to the isolation of an evolved strain fermenting cellobiose significantly faster than the parental strain. The evolved strain displayed enhanced ethanol yield from 0 to 0.4 g ethanol/g cellobiose. The evolved P. stipitis simultaneously fermented cellobiose and xylose in batch fermentation. The genetic information of our evolved P. stipitis would be valuable in the development of a microbial host for the production of biofuels and biomaterials from cellulosic biomass. 섬유소계 바이오매스로부터 바이오 연료 등과 같은 유용한 물질을 생산하기 위해서는 바이오매스로부터 유래하는혼합당을 효과적으로 대사할 수 있는 균주의 개발이 필수적이다. 본 연구에서는 xylose를 대사가 가능한 효모인 P. stipitis를 적응진화하여 cellobiose 대사효율이 향상되고cellobiose와 xylose를 동시에 대사할 수 있는 균주를 개발하고자 하였다. 총 10회의 계대배양을 통해 얻어진 진화된 P. stipitis 돌연변이 균주는 모균주에 비해 6배 이상 증가된cellobiose 대사속도를 나타내었으며 ethanol 생산수율을 0 에서 0.4 (g ethanol/g cellobiose)로 향상시켰다. 아울러 본실험에서 개발한 돌연변이 균주는 cellobiose와 xylose 혼합당 조건에서 모균주에 비해 2배 가까이 향상된 ethanol 생산 및 생산속도를 나타내었다.