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이호준(Ho Jun Lee),원두일(Doo Il Won),윤기봉(Kee Bong Yoon) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
Hydrogen embrittlement sensitivity analysis was conducted for candidate materials of nuclear hydrogen plant such as Alloy 617, SUS316L, Hastelloy C-276. For this study, electrochemical hydrogen charging technique was employed. Hydrogen charging was conducted in 0.5M H₂SO₄ solution and current density of 100 ㎃/㎠ was identically applied to all of the specimens. For the hydrogen charged specimens, hydrogen concentration was examined. From the result of hydrogen concentration analysis, it is found that SUS 316L has the lowest value of hydrogen concentration compare with the other candidate materials. To evaluate the sensitivity of hydrogen embrittlement additional study on strength assessment should be conducted.
김정은 ( Jung Eun Kim ),박찬하 ( Chan Ha Park ),오대석 ( Dae Seok Oh ),이승연 ( Seung Yeon Lee ),장세훈 ( Se Hun Jang ),홍지연 ( Jee Yeon Hong ),민혜진 ( Hye Jin Min ),박수아 ( Su Ah Park ),원두현 ( Doo Hyun Won ),박수남 ( Soo Nam 대한화장품학회 2011 대한화장품학회지 Vol.37 No.4
본 연구에서는 대황 추출물의 항산화 활성, 타이로시네이즈(tyrosinase) 저해 활성을 확인하였다. 대황의 50 % 에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획으로 실험을 진행하였다. 대황 추출물들의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성(FSC<sub>50</sub>)은 대표적인 항산화제인 (+)-α-tocopherol보다 낮은 것으로 나타났다. Luminol 발광법을 이용한 Fe<sup>3+</sup>-EDTA/H<sub>2</sub>O<sub>2</sub> 계에서 생성된 활성산소종에 대한 아글리콘 분획의 소거활성(총 항산화능, OSC<sub>50</sub>)은 0.265 μg/mL로 매우 큰 활성을 나타내었다. 대황 추출물의 rose-bengal로 증감된 <sup>1</sup>O<sub>2</sub>에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적(1∼50 μg/mL)으로 증가하였으며, 특히 아글리콘 분획은 10 μg/mL 농도에서 τ<sub>50</sub>이 757.0 min으로 높은 세포 보호 활성을 나타내었다. 대황 추출물 중 아글리콘 분획의 타이로시네이즈 저해활성(IC<sub>50</sub>)은 11.20 μg/mL으로 226.88 μg/mL인 알부틴(arbutin)보다 큰 활성을 보여주었다. 이상의 결과들로부터 대황 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 아글리콘 분획의 현저한 항산화 작용 및 큰 타이로시네이즈 저해 효과로부터 이들 분획 또한 화장품원료로서 응용 가능성이 큼을 알 수 있었다. In this study, the antioxidative effects and inhibitory activities on tyrosinase of Rheum undulatum (R. undulatum) L. extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of R. undulatum L. were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC<sub>50</sub>) of R. undulatum L. extracts was lower than (+)-α-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities (OSC<sub>50</sub>) of aglycone fraction on ROS generated in Fe<sup>3+</sup>-EDTA/H<sub>2</sub>O<sub>2</sub> system using the luminol-dependent chemiluminescence assay showed the most prominent effect at a concentration of 0.265 μg/mL. The cellular protective effects of extract/fractions of R. undulatum L. on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1 ~ 50 μg/mL). Especially, aglycone fraction in 10 μg/mL concentration showed the most protective effect among extracts (τ<sub>50</sub>, 757.0 min). The inhibitory effects (IC<sub>50</sub>, 11.20 μg/mL) on tyrosinase of aglycone fraction was much higher than arbutin (226.88 μg/mL), known as a whitening agent. These results indicate that R. undulatum L. extracts can be used as antioxidant. Particularly, aglycone fraction of R. undulatum L. showed superior antioxdative activity and high inhibitory effect on tyrosinase. Therefore, aglycone fraction of R. undulatum L. could be applicable to new functional cosmetics.
벼ㆍ맥주보리 작부체계에서 돈분액비 연용이 맥주보리 생육과 토양 환경에 미치는 영향
이성태(Seong-Tae Lee),서동철(Dong-Cheol Seo),김은석(Eun-Seok Kim),송원두(Won-Doo Song),이원규(Won-Gyu Lee),허종수(Jong-Soo Heo),이영한(Young-Han Lee) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.3
To investigate the effect of continual pre-plant application of liquid pig manure (LPM) on malting barley growth, quality and soil environment in double cropping system of rice and malting barley, the liquid pig manure was applied after harvesting rice and malting barley for 3 years. Field experiment was designed with non-fertilizer, chemical fertilizer (CF) recommended by soil testing, rice (LPM 50%+CF 50%)+malting barley (CF 100%), rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%), rice (LPM 100%)+malting barley (CF 100%) and rice (LPM 100%)+malting barley (LPM 100%). By continuous application of LPM 100%, the contents of available P₂O5 and exchangeable K in the soil were increased. The available P₂O5 increased from 243 to 350 mg kg<SUP>-1</SUP> and exchangeable K was changed 0.31 to 0.44 cmolc kg<SUP>-1</SUP>. However, the contents of available P₂O5 and exchangeable K were not significant changes in rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%) plot. Bulk density of soil was not affected by application of LPM. The microbial density was high in order of bacteria > actinomycetes > fungi. The population of aerobic bacteria in rice (LPM 100%)+malting barley (LPM 100%) plot was higher than other plots. The ratio of aerobic bacteria/fungi and biomass C content were the highest in rice (LPM 100%)+malting barley (LPM 100%) plot. The yield of malting barley was increased 22% by increasing culm length, panicle length, No. of panicle and 1,000 grains as 358 kg 10a<SUP>-1</SUP> in rice (LPM 100%)+malting barley (LPM 100%) plot compared with 294 kg 10a<SUP>-1</SUP> in rice (CF 100%)+malting barley (CF 100%) plot. The content of β-glucan was low by 4.5 and 4.4% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot, respectively. The content of crude protein was the lowest by 8.2% in non-fertilizer and rice (CF 100%)+malting barley (CF 100%) plot and the quality of malting barley was good as within 11%.