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이옥재,강은숙,곽노태,안태영 ( Ok Jae Rhee,Eun Sook Kang,No Tae Kwag,Tae Young Ahn ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.1
The nitrification rate and environmental factors were estimated 10 times from August 1992 to October 1993 in Lake Soyang. The number of nitrifying bacteria and nitrification rate were measured with MPN and ^14C-dark incorporation method, respectively. The distribution of ammonia-oxidizing bacteria was 0.5-220MPNml^-1 (mean, 11MPNml^-1) and that of nitrite-oxidizing bacteria was 0.5-92MPNml^-1(mean, 9MPNml^-1). The range of nitrification was estimated 0.01-2.25㎍-Nl^-1day^-1(mean,0.28㎍-Nl^-1day^-1). It was shown through multiple regression analysis from all data that the major factor affecting to nitrification rate was water temperature and the minor factor was pH. The distribution of nitrification rate and ammonia-oxidizing bacteria were not correlated.
이옥재,이일규,박희진,안태영 ( Ok Jae Rhee,Il Gyu Lee,Hee Jin Park,Tae Young Ahn ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.1
The nitrification rate and environmental factors were estimated 12 times at 4 stations from March 1992 to January 1994 in Daechung reservoir. The number of nitrifying bacteria and nitrification rate were measured with MPN and ^14C-dark incorporation method, respectively. The distribution of ammonia-oxidizing bacteria was 0.5∼220MPNml^-1(mean, 13MPNml^-1) and that of nitrite-oxidizing bacteria was ND-42MPNml^-1(mean,3MPNml^-1). The range of nitrification rate was 0.01∼7.15㎍-Nl^-1day^-1(mean, 0.74㎍-Nl^-1day^-1) and the highest value was 7.15㎍-Nl^-1day^-1 at station 3 where lots of fishfarm were located. Being showed through multiple regression analysis from all data estimated, the distribution of nitrification was affected by change of ammonia concentration, but not affected by nitrifying bacterial population.
민상현,이옥재,안태영 ( Sang Hyun Min,Ok Jae Rhee,Tae Young Ahn ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.4
Nitrification and denitrification potential were detected in the sediment of Lake Soyang by ^14C-bicarbonate incorporation and acetylene blockage method, respectively. The range of nitrification potential was 0.01∼2.49㎍-N gdwt^-1day^-1 and that of denitrification potential was 0.08∼5.25㎍-N gdwt^-1day^-1. Nitrification potential was showed the highest value at station 4 where organic carbon was rich. It was a high value at station 1 and 2 and was a low value with deep. Denitrification potential was showed the lowest value at station 1 in which organic carbon content was poor and water depth was shallow and was showed the highest value at station 4. Nitrification was correlated positively with NH_4^+-N and denitrification was correlated positively with organic carbon. The inorganic nitrogen reduction was comparatively superior to the inorganic nitrogen oxidation at all stations. So it was assumed to loss of nitrogen source from sediment.
하수처리장 방류수에 존재하는 항생제 내성인자가 하천에 미치는 영향
장예진,유용재,설우준,차창준,이옥재,채종찬,Jang, Yejin,Yoo, Yong-Jae,Sul, Woo Jun,Cha, Chang-Jun,Rhee, Ok-Jae,Chae, Jong-Chan 한국미생물학회 2017 미생물학회지 Vol.53 No.4
하수처리장 방류수와 하천 중의 항생제 내성인자 분포에 대한 상관성을 분석하기 위해 방류수와 상류 하천수, 하류 하천수를 대상으로 항생제 내성유전자와 전파 관련 유전자를 조사하였다. 3개 지점에서 134~183개의 항생제 내성유전자(ARG) 및 전파 관련 유전자(MGE)가 검출되었으며, 1개의 16S rRNA 유전자에 대한 ARG 및 MGE 유전자의 상대적인 총 합이 0.063~0.422 copy로 분석되었다. ARG와 MGE의 수와 존재량은 방류수에서 가장 높게 검출된 반면, 총 세균 수는 가장 적게 검출됨으로서 하수처리 과정에서 사멸된 세균에 포함된 유전자들이 검출된 것으로 판단된다. 또한 MGE의 존재량 양상이 ARG의 존재량과 상관관계를 보임으로서 항생제 내성균들의 내성기작이 자연내성보다는 획득내성일 가능성을 제시하였다. The antibiotic resistant genes (ARG) and mobile genetic elements (MGE) were investigated with the effluent of waste-water treatment plant (WWTP), and river waters of upstream and downstream in order to elucidate the effect of effluent on antibiotic resistance in a natural river. Total numbers of 134~183 of ARG and MGE were detected and the abundance of ARG and MGE was 0.063~0.422 copies per one of 16S rRNA gene in three water samples. Effluent sample contained the highest amount of the total number and abundance of ARG and MGE whereas total viable cells were observed in the lowest amount among the three samples. This indicated that the genes were originated from cells died during the wastewater treatment process. In addition, the co-relationship of abundance between ARG and MGE suggested that acquired resistance was a prevalent mechanism among the antibiotic-resistant bacteria existing in WWTP.