승모판 협착증에 병발한 신동맥 색전증 1 예

채종찬,이만호,고동혁,정을순,김향,이상종,홍미재 대한내과학회 1989 대한내과학회지 Vol.36 No.2

Although embolic occlusion of a renal artery is not rare, correct diagnosis and appropriate treatment are often delayed. We recently experienced in a case of renal artery embolism associated with mitral stenosis 45-year-old male, who suffered from a sudden onset of severe intractable abdominal pain. Analgesics a and sedatives did not alleviate the pain. Pertinent but non-specific laboratory findings disclosed leukocytosis, proteinuria and an increased serum lactic dehydrogenase level. A renal scan showed delayed excretion of the right kidney and a renal arteriogram disclosed abrupt cutting of the segmental artery of the right kidney associated with a filling defect in the delayed phase. He discharged from the hospital against advice for further evaluation and observation of his condition.

평판배지법에 의한 4-chlorobenzoate 탈염소화 세균의 검색

채종찬,김치경,민경희,박경근 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.1

Pseudomonas sp. DJ-12와 genomic DNA로부터 pBluescript SK(+)를 vector로 사용하여 4CBA의 탈염소화 유전자를 E. coli XL1-Blue에 클로닝하여 E. coli CJ1을 얻었다. 재조합 플라스미드인 pCJ1에는 4CBA의 탈염소화 유전자를 포함하는 약 9.5 kb의 insert DNA가 삽입되어 있었으며, 1개씩의 ApaI, HindIII, KpnI 인식부위와 2개의 AccI 인식부위가 존재하였다. pCJ1으로부터 4CBA의 탈염소화 유전자를 포함하는 약 3.4 kb의 EcoRI-HindIII 절편을 다시 subcloning 하여 E. coli CJ101을 얻었다. 이 4CBA의 탈염소화 기능을 갖는 재조합 균주들은 4CBA가 첨가된 Cl^--free 평판배지에서 균을 배양한 후, 0.1 M AgNO_3 용액을 첨가할 때 균체 집락의 주위에 생성되는 AgCl precipitate의 양으로 간편하게 검색할 수 있었다. 이와 같은 검정방법은 1 mM의 4CBA가 포함된 Cl^--free 평판배지에서 균체를 2일간 배양한 후 0.1 M AgNO_3를 첨가하는 조건으로 표준화하였다. The gene responsible for dechlorination of 4-chlorobenzoate (4CBA) was cloned in E. coli XL1-Blue from Pseudomonas sp. DJ-12. The cloned cell of E. coli CJ1 had the hybrid pBluescript SK(+) plasmid, into which about 9.5 kb genomic DNA fragment of Pseudomonas sp. DJ-12 was inserted. The subclone of pCJ101 was constructed by inserting the 3.4 kb EcoRI-HindIII fragment of pCJ1 into the vector. Those cloned cells could be simply selected by halo formation around the colonies which was the precipitate of AgCl produced by reaction of AgNO_3 and chloride ion liberated by bacterial dechlorination of 4CBA. Such a plate assay method was standardized by the procedure that colonies grown for 2 days on the Cl^--free plate medium containing 1 mM 4CBA were flooded with 0.1 M AgNO_3 solution.

미생물의 탈염소화 작용에 의한 남분해성 염화방향족 오염물질의 분해

채종찬,김치경 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.2

난분해성 유기화합물의 일종인 염화 방향족화합물은 냉각제, 소화제, 페인트, 용매, 플라스틱류, 유압제, 제초제, 농약, 그리고 화학합성에 필요한 전구물질 등에 널리 사용된다. 이들은 친지질 특성을 가지므로 생물체의 세포막에 쉽게 흡착되며 먹이사슬에 의한 생물학적 농축과정을 통해 인간을 포함하는 각종 생물체에 축적된다. 그 결과 생물체의 세포막 구조가 변화되고 기능이 저해될 뿐더러 암과 돌연변이를 유발하고 「환경호르몬」으로서 생물체의 내분비게 기능을 교란하는 등 심각한 보건학적 그리고 환경생물학적 문제를 일으키고 있다. 염화 방향족화합물들은 벤젠고리 구조와 벤젠고리에 염소가 치환된 탄소-염소 결합을 공통적으로 가지고 있으며 벤젠고리에 치환된 염소의 수와 같은 수의 염소라도 붙어 있는 위치에 따라 난분해 특징이 결정된다. 염화 방향족화합물들의 분해를 위해서는 미생물에 의한 벤젠 구조의 개환과정과 함께 벤젠 고리구조로부터 염소 치환기를 제거하는 탈염소황 과정이 반드시 일어나야만 한다. 호기적 환경에서 미생물에 의한 탈염소화는 분해 초기단계에서 dehalogenase라는 효소에 의해 촉매되는 oxygenolytic, reductive, 그리고 hydrolytic catalysis에 의해 일어나거나, 분해 대사과정 중에 저절로 염소치환기가 떨어져 나가는 경우도 있다. 탈염소화 과정을 거쳐 분해하는 미생물들을 이용한 염솨 방향족 오염물질의 생물학적 분해방법은 이미 사용되고 있는 물리·화학적 방법보다 경제적이며 2차 오염의 부작용 없이 그 오염물질들을 매우 효과적으로 처리할 수 있다. 따라서 탈염소화 기작을 포함한 분해과정의 이해는 생물학적 분해의 기본적인 정보를 제공할 뿐더러 난분해성 환경 오염물질의 분해처리를 위하여 보다 집중적으로 연구해야 할 과제라고 할 것이다. Chlorinated aromatic compounds are one of the largest groups of environmental pollutants as a result of world-wide distribution by using them as herbicides, insecticides, fungicides, solvents, hydraulic and heat transfer fluids, plasticizers, and intermediates for chemical synthesis. Because of their toxicity, persistence, and bioaccumulation, the compounds contaminated ubiquitously in the biosphere has attracted public concerns in terms of serious influences to wild lives and a human being, such as carcinogenicity, mutagenicity, and disturbance in endocrine systems. The biological recalcitrance of the compounds is caused by the number, type, and position of the chlorine substituents as well as by their aromatic structures. In general, the carbon-halogen bonds increase the recalcitrance by increasing electronegativity of the substituent, so that the dechlorination of the compounds is focused as an important mechanism for biodegradation of chlorinated aromatics, along with the cleavage of aromatic rings. The removal of the chlorine substituents has been known as a key step for degradation of chlorinated aromatic compounds under aerobic condition. This can occur as an initial step via oxygenolytic, reductive, and hydrolytic mechanisms. The studies on the biochemistry and genetics about microbial dechlorination give use the potential informations for microbial degradation of xenobiotics contaminated in natural microcosms. Such investigations might provide biotechnological approaches to solve the environmental contamination, such as designing effective bioremediation systems using geneticallly engineered microorganisms.

복부초음파로 의심된 간낭종의 임상적 관찰

채종찬,김영희,김향,박령철,이만호,정을순,이상종 大韓消化器病學會 1987 大韓消化器病學會誌 Vol.19 No.1

유독성 4-Chlorobiphenyl의 생분해를 위한 탈염소화 유전자의 클로닝

김치경,채종찬,한재진,Kim, Chi-Kyung,Chae, Jong-Chan,Han, Jae-Jin 한국미생물학회 1994 미생물학회지 Vol.32 No.2

4-Chlorobiphebyl(4CB)를 분해한 Pseudomonas sp. DJ-12가 가지고 있는 pcbABCD 유전자를 Escherichia coli에 클로닝한 결과, 재조합 균주인 E. coli CU1과 CU101은 모두 Pseudomonas sp. DJ-12에서와 같이 4CB를 분해하여 2,3-dihydrozybiphenyl(2,3-DHBP)을 생성하는 탈염소화 기능을 보여주었다. 특히 pcbAB를 포함하는 재조합 플라스미드인 pCU101을 가지고 있는 E. coli CU101은 Pserudomonas sp. DJ-12에서와 같이 4CB로부터 4-chlorobenzoic acid를 생성하지 않고 2, 3-DHBP만을 생성하는 탈염소화 기능을 보여주었다. 그러므로 Pseudomonas sp. DJ-12의 염색체 DNA로부터 클로닝한 약 2.2kb의 pcbAB 유전자는 4CB로부터 2,3-DHBP만을 생성하는 탈염소화기능을 가지고 있음이 밝혀졌다. The pchABCD genes in Pseudomonas sp. DJ-12 speciyin degradation o 4-chlorobiphenyl(4CB) were cloned in Eschericia coli. The cloned cells of E. coli CU1 and CU101 showed to produce 2,3-dihydroxybiphenyl (2,3-DHBP) from 4-chlorobiphenyl by dechlorination, as Pseudomonas so. DJ-12 produced 2,3-DHBP from both biphenyl and 4CB. In particular, E. coli CU101 transformed with the recombinant plasmid of pCU101 revealed dechlorination activity to produce 2,3-DHBP from 4CB without production of 4-chlorobenzoic acid. Therefore, the pcbAB genes (2.2 kb in size) cloned from the chromosome of Pseudomonas sp. DJ-12 were found to have dechlorination activity on 4CB to produce 2,3-DHNP.

Addison씨 병의 1예

정인교,채종찬,이만호,김철우,이상종,전우규 대한내분비학회 1987 Endocrinology and metabolism Vol.2 No.1

Addison's disease is a rare disorder resulting from chronic deficiency of adrenal cortical hormone concerned with gluconeogenesis and miberal metabolisim. The first comprehensive report of the clinical picture of this disease was provided by Addison in 1885, although a fairly accurate clinical and pathologic account of a case was presented by Bright twenty-five years earlier. Addison's disease is a relatively rare disorder, especially in Korea, but has been increasing in incidence with improvement of diagnostic method. We have experienced a typical case of Addison's disease confirmed by hormonal study, which was probably caused by tuberculosis. The patient who was a 37-year-old male was presented with dark-brown pigmentation of skin and mucous membrance, general weakness, anorexia, and weight loss. On physical examination he was moderately undernourished. The blood pressure was 100/60 mm Hg and pulse rate 96/min. Skin and mucous membrane were diffusely dark brown, especially over the face, gingiva, both elbows, knees and palms. The chest X-ray film showed ill defined round hazy density on the left middle lung field and laboratory findings revealed slightly decreased serum Na/K ratio, significantly decreased 24 hour urinary 17 ketosteroid and 17 hydroxycorticoids, and markedly elevated ACTH. A 24 hour ACTH stimulation test showed failing to rise the 24 hour urinary 17 ketosteroid and 17 hydroxycorticoids, and plasma cortisol levels. The patient's symptom and sign responded rapidly to glucocorticoid and antituberculosis therapy initially.

肝硬變症이 血淸 insulin 및 C-peptide에 미치는 影響

姜晋鎬,蔡種贊,朴鈴徹,李承世,鄭乙順,李祥鍾 高麗病院 1987 高醫 Vol.10 No.-

음식물쓰레기 분해능을 갖는 효소활성 균주의 분리 및 동정

정한샘,채종찬,이승제 全南大學校 農業科學技術硏究所 2016 農業科學技術硏究 Vol.53 No.-

Food waste composed of 30-60% starch, 10-40% lipids and 5-10% proteins(w/w). The increase of food waste is one of the big problem which caused world economic loss and environmental damage. In this study, for the purpose of decomposing food waste, the strain was screened from Korean traditional Nuruk and food waste. Twenty three strains were isolated from 74 isolates of Nuruk, and eleventh strains were isolated from food waste. Among them, 8 kinds of strains which have salt resistance and heat resistance and two or more enzymatic activities among the amylase, cellulase, lipase, protease were finally isolated and subjected to molecular biology identification, were identified such as Bacillus amyloliquefaciens, Acinetobactor baumannii, Enterobacter hormaechei, Saccharomycopsis fibuligera, Raoultella ornithinolytics, Serratia marcescens, Raoultella ornithinolytica. Six species of mixed strains were inoculated into food waste, and the result after 24 hours decomposition, the reduction of food waste was 52.7% on as biomass basis.

Barrett's Esophagus의 임상적 고찰

김철우,채종찬,박영철,이만호,정을순,이상종,김명숙 大韓消化器病學會 1988 大韓消化器病學會誌 Vol.20 No.2

원발성 간암과 췌장암에 있어서의 αFP, TATI 및 CA 19-9의 임상적 의의

서영주,채종찬,이승세,이만호,정을순,이상종 大韓消化器病學會 1988 大韓消化器病學會誌 Vol.20 No.3