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여윔증상 넙치 및 사육수 내 검출된 점액포자충(Enteromyxum leei and Parvicapsula anisocaudata)의 정량적 분석
이영준 ( Young Juhn Lee ),전려진 ( Lyu Jin Jun ),김예지 ( Ye Ji Kim ),한지은 ( Ji Eun Han ),이응준 ( Eung Jun Lee ),정준범 ( Joon Bum Jeong ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2
Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata) were performed using real-time PCR on the internal organs (head kidney, body kidney, intestine, spleen, brain, liver, heart, muscle, blood, and eye) of emaciated Paralichthys olivaceus from farm-A. The highest DNA copy number of E. leei was shown in the intestine (1.3 × 10<sup>8</sup> copies/mg tissue) of emaciatied P. olivaceus and DNA copy number in the other internal organs (1.3 × 10<sup>3</sup>~4.6 × 10<sup>5</sup> copies/mg tissue) showed lower than in intestine. From the result of real-time PCR for P. anisocaudata, it was considered mildly infected, due to the low DNA copy numbers of the head kidney (1.3 × 10<sup>3</sup> copies/mg tissue) and body kidney (9.1 × 10<sup>3</sup> copies/mg tissue). In order to investigate whether myxosporean parasites can be detected in a non-invasive way, quantitative analysis of E. leei and P. anisocaudata from rearing water of three farms were performed by real-time PCR. The DNA copy number of E. leei from rearing water of farm-A and farm-B were 8 × 104 and 5 × 10<sup>5</sup> copies/L, respectively. However, it was not detected in farm-C. For P. anisocaudata from rearing water, farm-A, farm-B and farm-C showed 0, 2.0 × 10<sup>6</sup> and 5.1 × 10<sup>6</sup> copies/L, respectively.
양식 넙치에서 분리한 어병세균의 lincomycin에 대한 내성 유전자의 분포
김예지,전려진,이영준,고예진,한소리,김성현,정준범,Kim, Ye Ji,Jun, Lyu Jin,Lee, Young Juhn,Ko, Ye Jin,Han, So Ri,Kim, Sung Hyun,Jeong, Joon Bum 한국어병학회 2022 한국어병학회지 Vol.35 No.1
Lincomycin as one of the lincosamides antibiotics have been mainly used in human and livestock fields, but have not been used in aquaculture. In this study, the distribution of minimum inhibitory concentration (MIC) values against lincomycin and the detection of the macrolide-lincosamide-streptogramin (MLS) resistance gene were confirmed in bacterial pathogens isolated from cultured olive flounder (Paralichthys olivaceus). Of the 107 strains isolated from Jeju, 36 strains of Gram-positive bacteria and 71 strains of Gram-negative bacteria were identified. Most of Streptococcus spp. was found to have a MIC value of less than or equal to 0.5 ㎍/mL, and Edwardsiella piscicida was found to have a MIC value higher than 1,024 ㎍/mL. V. harveyi and V. alginolyticus mostly showed MIC values of 256 ㎍/mL, but V. scophthalmi displayed values of 8~64 ㎍/mL. In the detection of MLS resistance gene, erm(B) was detected in 9 strains of Streptococcus spp., and erm(A) was confirmed in one strain.