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이상엽,장호남,Pyung Cheon Lee,Soon Ho Hong 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.2
A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciproducens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKs.
원발성개방각녹내장 환자의 백내장수술 중 나타난 악성녹내장 유사 현상 1예
이상엽,박종운,Sang Yeop Lee,Jong Woon Park 대한안과학회 2010 대한안과학회지 Vol.51 No.8
Purpose: Malignant glaucoma is a rare complication of anterior segment surgery. The authors present a case of a malignant glaucoma-like phenomenon during cataract surgery in a primary open-angle glaucoma (POAG) patient. Case summary: A 74-year-old man with POAG underwent a cataract surgery in his left eye. After phacoemulsificiation, sodium hyaluronate was used to form the capsular bag for intraocular lens implantation. Following this procedure, the capsular bag space and anterior chamber became shallow. Intraocular pressure (IOP) elevated, and the shallowing of the anterior chamber and capsular bag space continued to a dangerous level. The surgical procedure was stopped and postponed for two days. After two days, IOL was successfully implanted in the posterior bag. Conclusions: Although the preoperative ophthalmologic examination failed to reveal a zonular problem, there was a possibility for a small area of zonular defect. A malignant glaucoma-like phenomenon was suspected to have occurred due to leakage of sodium hyaluronate or balanced salt solution into the vitreous through a small area of zonular dialysis. If a similar condition, occurs delayed surgery for IOL implantation would be more effective than vitrectomy or laser treatment. J Korean Ophthalmol Soc 2010;51(8):1150-1154
전문(全文) DB 구축(構築)에 의한 한국통신연구정보관리(韓國通信硏究情報管理) 시스템 개발(開發)
이상엽,안현수,이양옥,Lee, Sang-Yeob,Ahn, Hyun-Soo,Lee, Yang-Ok 한국과학기술정보연구원 과학기술정보센터 1993 Journal of Information Science Theory and Practice Vol.24 No.1
한국통신(韓國通信) 연구개발단(硏究開發團)에서는 사내(社內) 각 연구부서(硏究部署)에서 발생하는 각종 최신 연구정보(硏究情報)의 원문(原文)을 축적(蓄積) 공동활용(共同活用)하기 위하여 한국통신연구정보관리(韓國通信硏究情報管理) 시스템(KTRIMS)을 개발(開發)하였으며, 본(本) 고(稿)에서는 KTRIMS의 구성(構成)과 특징(特徵)을 중심(中心)으로 기술(記述)하였다. KTRC(Korea Telecom Research Center) has developed the KTRIMS(Korea Telecom Research Information Management System) to keep and share the full text of the various up-to-date research information which many research institutes in KT have produced. This paper has presented the structure and the features of the KTRIMS.
Development of DNA Microarray for Pathogen Detection
이상엽,장경희,유내춘,김준명,금기창,최준용,Seung Min Yoo,유소영,Won Min Yoo,Duke Lee 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.2
Pathogens pose a significant threat to humans, animals, and plants. Consequently, a considerable effort has been devoted to developing rapid, convenient, and accurate assays for the detection of these unfavorable organisms. Recently, DNA-microarray based technology is receiving much attention as a powerful tool for pathogen detection. After the target gene is first selected for the unique identification of microorganisms, species-specific probes are designed through bioinformatic analysis of the sequences, which uses the information present in the databases. DNA samples, which were obtained from reference and/or clinical isolates, are properly processed and hybridized with species-specific probes that are immobilized on the surface of the microarray for fluorescent detection. In this study, we review the methods and strategies for the development of DNA microarray for pathogen detection, with the focus on probe design.
단안상전장애에 동반된 눈꺼풀처짐에서 눈꺼풀올림근을 이용한 이마근걸이술 1예
이상엽,김혜영,Sang Yeop Lee,Hye Young Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.2
Purpose: For the correction of ptosis in monocular elevation deficiency (MED) patients, levator resection or frontalis sling operation with various materials has been used. The authors present a case of a monocular elevation deficiency patient who was treated for ptosis using the levator muscle as a frontalis sling. Case summary: A six-year-old male patient with left monocular elevation deficiency underwent a frontalis sling operation using a levator muscle. The patient had received left inferior rectus recession, left medial rectus resection and left lateral rectus recession with transposition. An upper eyelid skin crease incision was made, and the anterior surface of the levator muscle was exposed as far as the Whitnall ligament. A levator muscle flap was then formed from aponeurosis by incising the full width of the levator horizontally, just below the Whitnall ligament and anchored to the frontalis muscle. The surgical result was satisfactory as the preoperative MRD1 was 0.5 mm and the postoperative MRD1 was 2.5 mm. There were no complications such as keratitis or wound problems. Conclusions: The levator muscle flap can be an effective frontalis suspension material to correct ptosis in an MED patient with poor levator function. J Korean Ophthalmol Soc 2010;51(2):282-285