심포지움 : 약제에 의한 의인성반응 : 혈액계에의 영향

이규보 ( Gyu Bo Lee ) 대한내과학회 1984 대한내과학회지 Vol.27 No.5

방사성핵종 요류역학검사를 이용한 하부요로폐색의 평가

이규보(Kyu Bo Lee),김광원(Kwang Won Kim),이재태(Jae Tae Lee),황기석(Kee Suk Whang),윤여득(Yeo Deuk Yoon),손상균(Sang Kyun Sohn),정진홍(Jin Hong Chung),손형규(Houng Gyu Sohn),정성광(Sung Kwang Chung) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.1

N/A Various urodynamic studies have been used in patients with bladder outlet obstruction in order to evaluate the degree of obstruction, the results of therapy and postprostatectomy conditions. Radionuclide urodynamic study was performed in 27 patients with bladder outlet obstruction and 30 normal controls. The parameters evaluated were voiding time, 50% voiding time, average flow rate, peak flow rate, corrected peak flow rate, ejection fraction of the bladder and residual urine. Voiding time, 50% voiding time and residual urine of patients were significantly larger than controls and average flow rate, peak flow rate, peak corrected flow rate and ejection fraction were significantly lower in patients. This method was noninvasive procedure for determining of voiding parameters and it avoids the extraexamination needs to determine the residual urine.

자가면역성 갑상선질환에서 혈청 가용성 Interleukin-2 Receptor(IL-2R)치와 Neopterin치에 관한 연구

이인규 ( Lee In Gyu ),김정철 ( Kim Jeong Cheol ),이상길 ( Lee Sang Gil ),이재태 ( Lee Jae Tae ),이규보 ( Lee Gyu Bo ) 대한내과학회 1992 대한내과학회지 Vol.42 No.3

Activated lymphocytes release a soluble form of interleukin-2 receptor. Increased serum levels of soluble IL-2R have been notedina variety of autoimmune diseases and in conditions associated with activated cell-mediated immunity. Activated T-cells also release a interferon-gamma that is capable of activating a guanosine triphoshate (GTP) cyclohydrolase in macrophages leading to release of neopterin. Thus, soluble IL-2R and neopterin can be utilized as markers for the clinical assessment of cell-mediated immune responses. This study was undertaken to investigate the cell-mediated immune responses in vivo of patients with autoimmune thyroid disease. Serum levels of soluble IL-2R and neopterin were measured in 46 patients with Graves` disease and 12 patients with Hashimoto`s thyroiditis. Serum soluble IL-2R was measured by an enzyme linked immunoassay and serum neopterin was measured by a radioimmunoassay. The levels of serum soluble IL-2R were markedly increased in untreated, hyperthyroid patients with Graves` disease. However the serum neopterin levels were not elevated in Graves` diseases in spite of an increase in serum soluble IL-2R. The levels of serum soluble IL-2R and neopterin were normal in untreated patients with Hashimoto`s thyroiditis. In patients with Graves` diseaes, once hyperthyroidism was controlled by antithyroid drugs, the levels of serum IL-2R were decreased. And the levels of serum soluble IL-2R correlated significantly with the levels of serum total T3 and T4 in patients with Graves` disease. However, levels of serum neopterin in Graves` disease were not significantly changed despite of antithyroid treatment. Levels of serum soluble IL-2R and neopterin in patients with Hashimoto`s thyroiditis were not changed. Thus, in vivo measurement of serum soluble IL-2R released from activated T lymphocytes is a useful immunological indicator of disease activity in Graves` disease.

당뇨병 환자에서 방사성핵종 요류역학 검사를 이용한 배뇨기능의 평가

정병천 ( Jeong Byeong Cheon ),최정일 ( Choe Jeong Il ),김광원 ( Kim Gwang Won ),이재태 ( Lee Jae Tae ),이규보 ( Lee Gyu Bo ),김성국 ( Kim Seong Gug ),김보완 ( Kim Bo Wan ),이인규 ( Lee In Gyu ) 대한내과학회 1992 대한내과학회지 Vol.42 No.3

Background: Patients suffering from diabetes mellitus for long duration are known to have serious complications involving various organs. Diabetic cystopathy occurs in 43% to 87% of all diabetic patients and is closely related with development of the diabetic neuropathy Cystometry, sphincter electromyography, uroflowmetry, urethral pressure profilometry and electro physiologic test are applied in assessing diabetic cystopathy. But these methods are invasive and inadequate procedures for repetitive usage. Methods: The radionuclide urodynamic study using a gamma camera and ^(99m)Tc-DTPA) is more convenient and a non-invasive test than the above mentioned tests and it allows the measuremnt of residual urine without urinary catheterization. We have performed 93 radionuclide urodynamic examinations in 62 diabetic men and 31 healthy men for diagnosis of urinary bladder dysfunction. Results: The results obtained were as follows. 1) Urinary bladder function of the diabetic was shown to be remarkably deteriorated when compared to normal men. Voiding time (VT), 50% voiding time (50% VT), average flow rate (AFR), peak flow rate (PFR), corrected peak flow rate (C.PFR) and ejection fraction (EF) of patients developed neuropathy were significantly different when compared to healthy men, but it was well preserved in those without neuropathy. Futhermore, residual urine volume (RV) and 50% VT seemed to be the most sensitive parameters for diagnosis of urodynamic deragments of diabetes. 2) In patients with proliferative diabetic retinopathy, RV and 50% VT were higher, and AFR, C-PFR and EF were lower than those with no retinopathy. 3) 50% VT had positive correlation with diabetic duration, and AFT, PFR, C-PFR, negative correlatons. Conclusion: Urinary bladder dysfunction was noted in patients with diabetes and it was more severe in patients with neuropathy, retinopathy and a long histroy of diabetes. The radionuclide urodynamic study can be used for diagnosis of diabetic cystopathy and for gollowup evaluations after treatment.

제3차 대한간학회 춘계학술대회 초록집 : Tc-99m Galactosyl-Human Serum Albumin(GSA)을 이용한 간 Asialoglycoprotein수용체영상술: TAA에 의한 간손상 생쥐에서의 분포상

이재태 ( Lee Jae Tae ),천경아 ( Cheon Gyeong A ),권영오 ( Kwon Yeong O ),김성국 ( Kim Seong Gug ),최용환 ( Choe Yong Hwan ),이규보 ( Lee Gyu Bo ),정준모 ( Jeong Jun Mo ) 대한간학회 1997 Clinical and Molecular Hepatology(대한간학회지) Vol.3 No.2(S)

골수이형성증후군의 임상혈액학적 고찰

손상균 ( Son Sang Gyun ),정병천 ( Jeong Byeong Cheon ),김광원 ( Kim Gwang Won ),이재태 ( Lee Jae Tae ),이규보 ( Lee Gyu Bo ),황기석 ( Hwang Gi Seog ) 대한내과학회 1990 대한내과학회지 Vol.38 No.6

N/A Myelodysplastic syndrome is a primary hematologic disease characterized by ineffective hematopoiesis with peripheral blood cytopenias. The authors conducted a clinicohematologic observation of 55 cases of MDS from July 1983 to June 1988. The result are as follows. The peak age incidence was in the fifth decade, and the male-to-female ratio was nearly 1:1. RA was observed most frequently. Thereafter RAEB-T, RAEB, RARS and CMML were observed, respectively. The chief complaints of the patients were exertional dyspnea and fatigability by anemia. Physical findings showed pallor (85.5%), purpura (40.0%), retinal hemorrhage (38.2%), hepatomegaly (18.2%), lymphadenopathy (14.5%), icteric sclera (12.7%) and splenomegaly (10.9%). The common infection sites were oral (38.0%), systemic (sepsis: 20:0%) and lung (14.5%). The main hematologic changes were pancytopenia (56.4%) and then anemia and thro.mbocytopenia, anemia and leukopenia, anemia and thrombocytopenia and leukocytosis, anemia only was present in 20.0%, 10.9%, 7. 5% and 5.4%, respectively. The laboratory findings showed increased serum iron (48.1%), decreased serum ferritin (43.1%), normal LAP scores (89.1%), prolonged plasm iron disappearance time (48.3%), shortened PID (6.9%), increased LDH (56.3%), increased vitamin B12 (29.5%) and decreased folic acid (4.5%). The bone marrow finding showed nearly hypercellular (38.2%) or normocellular (45.5%), along with variable morphological abnormalities, such as dyserythropoiesis, dysgranulopoiesis and dysmegakaryopoiesis. Cytogenetic studies showed abnormal chromosomal findings in five out of 13 cases. With combination therapy using androgen, pyridoxine and corticosteroid in 29 cases of RA and RARS, 31% of patients were clinically improved. And with low dose Ara-C therapy in 15 cases of RAEB, CMML and RAEBT, 26.6% of cases were improved. In the cases of RAEB and RAEB-T, nine cases of RAEB and RAEB-T converted to an other subtype of MDS or leukemia within one to six months. Nine cases expired (sepsis in five cases and CVA in four cases) during the observation period.

수종의 암세포에서 Verapamil이 Tc-99m MIBI와 Tetrofosmin의 섭취에 미치는 영향

김대현 ( Kim Dae Hyeon ),유정아 ( Yu Jeong A ),서명랑 ( Seo Myeong Lang ),배진호 ( Bae Jin Ho ),정신영 ( Jeong Sin Yeong ),안병철 ( An Byeong Cheol ),이규보 ( Lee Gyu Bo ),이재태 ( Lee Jae Tae ) 대한핵의학회 2004 핵의학 분자영상 Vol.38 No.1

목적: 다약제내성(MDR) 극복제 verapamil은 MDR이 발현된 암세포에서 Tc-99m MIBI(MIBI)와 tetrofosmin(TF)의 섭취를 증가시키는 것으로 알려져 있으나, 세포의 종류에 따라서는 MIBI와 TF의 섭취를 감소시킬 수 있다는 보고가 있다. 본 연구는 암세포의 종류에 따라 verpamile이 MIBI와 TF의 섭취에 미치는 영향이 세포에 따른 차이가 있는지를 알아보고, 이러한 차이가 세포독성이나 PKC효소의 발현에 따른 차이인가를 알아보았다. 방법: 백혈병세포 K562세포와 유방암세포 MCF7, 난소암세포 SK-OV-3은 세포 및 MDR이 유발된 K562(Adr)세포를 배양하였다. 시험관에서 1×10^(6) cells/㎖ 농도의 single-cell suspension 상태로 분주하고 verapamil을 1, 10, 50, 100, 200 μM의 농도로 처리한 후 MIBI와 TF를 배양한 후, 37℃에서 1, 15, 30, 45, 60분 동안 반응시킨 후 pellet과 supernatant의 방사능 치를 감마계수기로 측정하여 투여한 방사능 치에 대한 세포내 섭취백분율로 표시하였다. Verapamil의 세포독성은 MTT assay로 측정하였고, 세포내의 PKC isotypes의 변화는 western blotting analysis로 평가하였다. 결과: 4종류의 세포 모두에서 MIBI와 TF의 섭취는 1, 15, 30, 45, 60분 배양 시간에 따라 증가하였다. verapamil을 처리시 다약제 내성이 유발된 K562(Adr)세포에서 100 μM의 농도까지는 MIBI와 TF 섭취가 증가하였고, 최대 50 μM에서 10배 증가하였다. 그러나 K562세포를 verapamil 1 μM로 처리하였을 때는 기저치와 유사하였으나, verapamil의 농도가 증가함에 따라 MIBI와 TF의 세포섭취는 모두 감소하였다. K562세포의 60분 MIBI 섭취율은 79%(10 μM), 47%(50 μM), 29%(100 μM), 60%(50μM), 42%(100 μM), 2.7%(200 μM)로 감소하였다. MCF7, SK-OV-3세포에서는 verapamil 10 μM까지는 MIBI와 TF의 섭취가 기저치와 유사하거나 소량 증가하였으나 50 μM이상의 용량에서는 감소하여 100 μM에서는 각각 40%와 5%만 섭취되었다. MTT assay상 K562(Adr)세포에서는 verapamil 100 μM 이상에서는 유의하게 낮았으나 다른 세포는 200 μM까지에도 차이가 없었다. PKC 아형의 분석상 PKC ε이 K562(Adr)세포에서 많이 발현되었으나, K562와 K562(Adr)세포에서는 verapamil처리에 따른 PKC 아형의 변화는 없었다. 결론: Verapamil은 암세포의 종류에 따라 MIBI와 TF의 섭취를 감소시켰고, 고용량에는 MDR 세포의 섭취도 감소시켰으며 이러한 현상은 세포독성이나 PKC효소 아형과는 관련이 없었다. 그러므로 MDR의 진단시 verapamil을 처치에 따른 MIBI와 TF의 섭취 정도를 기준으로 하는 경우에는, verapamil의 농도와 세포의 종류에 따라 현저한 차이가 있을 수 있다는 점을 생각하여야 한다. Purpose: Cellular uptake of ^(99)mTc-sestamibi (MIBI) and ^(99)mTc-tetrofosmin (TF) is low in cancer cells expressing multidrug resistance(MDR) by p-glycoprotein(Pgp) or multidrug related protein(MRP). Verapamil is known to increase cellular uptake of MlBl in MDR cancer cells, but is recently reported to have different effects on tracer uptake in certain cancer cells. This study was prepared to evaluate effects of verapamil on cellular uptake of MlBl and TF in several cancer cells. Materials and Methods: Cellular uptakes of Tc-99m MlBl and TF were measured in erythroleukemia K562 cell, breast cancer MCF7 cell, and human ovarian cancer SK-OV-3 cells, and data were compared with those of doxorubicin-resistant K(562(Ad) cells. RT-PCR and Western blot analysis were used for the detection of mdrl mRNA and Pgp expression, and to observe changes in isotypes of PKC enzyme. Effects of verapamil on MlBl and TF uptake were evaluated at different concentrations upto 200 μM at 1×10^(6) cells/^(㎖) at 37℃. Radioactivity in supernatant and pellet was measured with gamma counter to calculate cellular uptake ratio. Toxicity of verapamil was measured with MTT assay. Results: Cellular uptakes of MlBl and TF were increased by time in four cancer cells studied. Co-incubation with verapamil resulted in an increase in uptake of MlBl and TF in K562(Adr) cell at a concentration of 100 μM and the maximal increase at 50μM was 10-times to baseline. In contrast, uptakes of MlBl and TF in K562, MCF7, SK-OV3 cells were decreased with verapamil treatment at a concentration over 1μM. With a concentration of 200μM verapamil, MlBl and TF uptakes in K562 cells were decreased to 1.5% and 2.7% of those without verapamil, respectively. Cellular uptakes of MlBl and TF in MCF7 and SK-OV-3 cells were not changed with 10μM, but were also decreased with verapamil higher than 10μM, resulting 40% and 5% of baseline at 50μM. MTT assay of four cells revealed that K562, MCF7, SK-OV3 were not damaged with verapamil at 200μM. Conclusion: Although verapamil increases uptake of MlBl and TF in MDR cancer cells, cellular uptakes were further decreased with verapamil in certain cancer cells, which is not related to cytotoxicity of drug. These results suggest that cellular uptakes of both tracers might differ among different cells, and interpretation of changes in tracer uptake with verapamil in vitro should be different when different cell lines are used.

Waldenstrom's Macroglobulinemia 1 예

최성만,김재식,김중명,배한익,김용진,이인걸,이규보,김영태 대한병리학회 1983 Journal of Pathology and Translational Medicine Vol.17 No.3