노랑쐐기나방과 장수쐐기나방에 대한 곤충병원성 선충의 병원성

윤정범(Jung Beom Yoon),김형환(Hyeong Hwan Kim),김동환(Dong Hwan Kim),양창열(Chang Yeol Yang),서미혜(Mi Hye Seo),정영학(Young Hak Jung),이동운(Dong Woon Lee) 한국농약과학회 2018 농약과학회지 Vol.22 No.1

Recently the damage has increased not only fruit trees and flowers but also landscape trees, street trees and garden trees as the density of Monema flavescens and Latoia consocia increases. If stinging hairs of the larva or adult is touched the workers body, it is causesd inflammation and pain, in addition to plant damage. However the only insecticide is the chlorantraniliprole which is registered for the Monema flavescens in japanese apricots. Although many farmers use neem oil, Sophora flavescens, effective micro organisms, pyroligneous liquor, etc. the environmentally friendly control measure, it can not satisfy them with its low effectiveness. We tested the insecticidal property of Steinernema carpocapsae GSN1 strain (ScG) and Heterorhabditis sp. Gyeongsan strain (HsG) separated in Korea to control larvae of M. flavescens and L. consocia. As a result, ScG can effectively control larvae of M. flavescens and L. consocia with 10 concentration and at 6 PM in July.

국화재배지에 발생하는 꽃노랑총채벌레의 발육단계별 약제 감수성

윤정범(Jung Beom Yoon),조영식(Young Sik Cho),양창열(Chang Yeol Yang),서미혜(Mi Hye Seo) 한국농약과학회 2020 농약과학회지 Vol.24 No.2

This study was carried out to investigate insecticidal susceptibility of 10 registered insecticides to western flower thrips, Frankliniella occidentalis, which damage chrysanthemum in the Changwon area. Bioassays were conducted under laboratory by using collected thrips in chrysanthemum cultivation. Among them the insecticides containing benfuracarb and chlorfenapyr that showed 100% adults mortality 1 day after treatment. The larvae died 94-100% in insecticides containing benfuracarb, chlorfenapyr and emamectin benzoate. The pupae died 94-100% in insecticides containing benfuracarb, chlorfenapyr and spinetoram. It is important to use alternately insecticides benfuracarb, chlorfenapyr, emamectin benzoate and spinetoram to spray it sufficiently on above-ground part and subterranean of chrysanthemums for efficient control of western flower thrips that occur in Changwon area.

국내 양송이버섯 재배 중 발생하는 버섯파리류 분자생물학적 종 동정

윤정범,김형환,정충렬,강민구,권선정,김동환,양창열,서미혜,Yoon, Jung-Beom,Kim, Hyeong-Hwan,Jung, Chung-Ryul,Kang, Min-Gu,Kwon, Sun-Jung,Kim, Dong-Hwan,Yang, Chang-Yeol,Seo, Mi-Hye 한국응용곤충학회 2016 한국응용곤충학회지 Vol.55 No.4

버섯파리는 국내 양송이 균상 재배지에서 발생하여 버섯 균사와 자실체를 가해하고 간접적으로는 병을 매개하여 피해는 해마다 증가하고 있다. 부여군 세도면, 보령시 성주면, 용인시 남사면, 칠곡군 약목면 등 총 4개 지역의 버섯 재배사에서 끈끈이트랩을 이용하여 채집한 버섯파리 성충을 DNA barcoding 방법으로 Cytochrome Oxidase I (COI)의 염기서열 분석을 통해 종 동정을 실시하였다. 그 결과 4개 조사지역의 양송이에서 발생하는 버섯파리는 모두 긴수염버섯파리(Lycoriella ingenua)로 동정되었다. The dark-winged fungus gnats are one of the most serious fly pests attacking the mushroom cultivation in Korea. They cause severe damage to the artificial sawdust beds used to cultivate mushroom, and reduce the production of button mushroom, Agaricus bisporus, in greenhouses. In this study, we collected nine species of the mushroom flies in order to identify the dominant species of the dark-winged fungus gnat attacking the A. bisporus plantation using the yellow sticky trap in Buyeo-gun, Boryeong-gun, Yongin-si and Chilgok-gun from April to June 2015. The collected samples were used to determine the DNA sequence of the cytochrome c oxidase subunit I (COI) of the nine different species by DNA barcoding. The sequencing results showed that Lycoriella ingenua was the dominant dark-winged fungus gnat species destroying A. bisporus cultivated on the artificial sawdust beds in Korea.

보간을 근거로 한 디지털 이미지의 위, 변조 분석 연구

이정범 ( Jung Beom Lee ),윤용인 ( Yong In Yoon ),윤성빈 ( Sung Bin Youn ),두경수 ( Kyoung Soo Doo ),하동환 ( Dong Hwan Har ) 한국사진학회 2007 AURA Vol.0 No.16

논문 : 빛의 방향 분석을 기반으로 한 합성된 이미지 판독

이정범 ( Jung Beom Lee ),윤용인 ( Yong In Yoon ),두경수 ( Kyoung Soo Doo ),이상훈 ( Sang Hoon Lee ),하동환 ( Dong Hwan Har ) 한국사진학회 2006 AURA Vol.0 No.15

One of the most frequent methods of digital image composition is inserting image which does not exist in the original image. This kind of method is getting more elaborate because of improvement of digital processing technology. So, it is very difficult to judge that the image is true or fake by naked eyes. When we analyze the image which is suspected, we think that the object on the image was there or not firstly. In this study, we would analyze for direction of the light on image that detached from composed image.

시설재배 국화에서 총채벌레의 종 동정 및 보독 바이러스 동시 검출을 위한 다중 진단법 적용

윤주연(Ju-Yeon Yoon),윤정범(Jung-Beom Yoon),서미혜(Mi-Hye Seo),최승국(Seung-Kook Choi),조인숙(In-Sook Cho),정봉남(Bong-Nam Chung),양창열(Chang Yeol Yang),Venkata Subba Reddy Gangireddygari 한국식물병리학회 2020 식물병연구 Vol.26 No.4

이번 연구는 국화에서 문제되는 총채벌레의 종 동정 및 보독 바이러스인 토마토반점위조바이러스(Tomato spotted wilt virus, TSWV)를 동시에 확인할 수 있는 진단방법을 개발하였다. 이는 총채벌레 1마리에서 추출한 핵산에 꽃노랑총채벌레및 대만총채벌레의 ITS2 부분에 특이적인 프라이머와 TSWV 외피단백질(N) 유전자 특이적인 프라이머를 동시에 넣어 reverse tran scription‒polymerase chain reaction을 수행하여 DNA를증폭시키는 방법으로 전기영동하여 각각 287, 367, 777 bp의DNA 단편의 크기를 비교함으로써 총채벌레의 종 동정 및 총채벌레의 TSWV 보독 여부를 동시에 확인할 수 있다. 충청남도 태안 및 경상남도 창원의 국화 시설하우스에서 총채벌레를 포집하여 총채벌레 우점종과 총채벌레의 TSWV 보독율을 조사한결과, 태안의 국화 시설하우스에서는 꽃노랑총채벌레가 83.7% 로 우점하고 있으며 채집된 총채벌레 중 72.9%가 TSWV를 보독하고 있었으며, 창원에서는 꽃노랑총채벌레가 92.2%를 차지하고 있으며 84.0%의 총채벌레에서 TSWV가 진단되었다. 이러한결과는 Frankliniella occidentalis가 우점종이며 온실의 국화 식물에서 TSWV의 전반에 중요한 역할을 한다는 것을 확인해준다. 이번 연구는 국화 시설하우스에서 총채벌레를 통한 TSWV 의 시설하우스내 유입시기 및 확산 경로 등 바이러스의 역학연구를 위한 간편진단법으로 활용 가능함을 예시해준다. We have developed a simultaneous diagnostic method that can identify both the species of thrips and tomato spotted wilt virus (TSWV) that are problematic in chrysanthemum plants. This is a method of amplifying DNA by performing reverse transcription-polymerase chain reaction by simultaneously adding primers specific to TSWV coat protein (N) gene and primers specific to the internal transcribed spacer 2 region of Frankliniella occidentalis and F. intonsa using total nucleic acid extracted from one thrips. The sizes of DNA fragments for TSWV, F. occidentalis, and F. intonsa were 777, 287, and 367 bp, respectively. These results showed species identification of thrips and whether thrips carrying TSWV can be simultaneously confirmed. Further usefulness of the simultaneous diagnostic method was made from greenhouse survey at chrysanthemum greenhouses in Taean (Chungcheongnam-do) and Changwon (Gyeongsangnam-do) to investigate the identification of thrips species and the rate of thrips carrying TSWV. Of thrips collected from the greenhouses, 83.7% thrips was F. occidentalis and 72.9% F. occidentalis carried TSWV in Taean. Similarly, the diagnostic method showed that 92.2% thrips was F. occidentalis and 84.0% F. occidentalis carried TSWV in Changwon. These results confirm that F. occidentalis is a dominant thrips species and the thrips species plays a crucial role in the transmission of TSWV in chrysanthemum plants in the greenhouses. Taken together, this study showed a simple diagnostic method for thrips identification and epidemiological studies of the timing and spread of TSWV through thrips in chrysanthemum greenhouses in South Korea.

수경재배 딸기에서 포식성 천적, 아큐레이퍼응애를 이용한 작은뿌리파리의 생물적 방제

김형환,윤정범,김동환,양창열,강택준,Kim, Hyeong-Hwan,Yoon, Jung-Beom,Kim, Dong-Hwan,Yang, Chang-Yeol,Kang, Taek-Jun 한국응용곤충학회 2016 한국응용곤충학회지 Vol.55 No.4

2012년부터 2013년까지 2년에 걸쳐 남원과 합천의 수경재배 딸기에서 포식성 응애 아큐레이퍼응애를 배지에 방사하여 작은뿌리파리의 밀도억제 효과를 조사하였다. 수경재배 딸기에서 아큐레이퍼응애를 $m^2$당 60.6마리를 3회~4회 방사한 결과 아큐레이퍼응애 방사구에서는 작은 뿌리파리의 밀도억제 효과도 높았고 작은뿌리파리 유충에 의한 시들음 증상이나 고사되는 딸기의 피해도 줄었는데 무처리구 보다 7.4~10.4%의 피해율이 감소되었다. Biological control of the fungus gnat Bradysia difformis by the predatory mite Hypoaspis aculeifer was examined in hydroponic systems of strawberries in Namwon and Hapcheon varieties of Jeollabuk-do and Gyeongsangbuk-do provinces, respectively, from 2012 to 2013. The damage to strawberries caused by B. difformis was examined in seedling stages and during cultivation periods. To achieve environment-friendly management of B. difformis, 60.6 individuals of H. aculeifer were released per square-meter three and four times during cultivations periods of the Namwon and Hapcheon strawberry varieties, respectively. H. aculeifer had better control efficiency against of B. difformis than that observed with conventional cultural practices. Wilt symptoms and damage of strawberries due to B. difformis were decreased by approximately 7.4-10.4%.

시설토마토에 발생하는 담배가루이 방제를 위한 허브식물의 유인효과

서미혜,양창열,신용습,윤정범,최병렬,박정준,Seo, Mi Hye,Yang, Chang Yeol,Shin, Yong Seub,Yoon, Jung Beom,Choi, Byeong Ryeol,Park, Jung-Joon 한국환경생물학회 2020 환경생물 : 환경생물학회지 Vol.38 No.4

담배가루이 방제는 대부분 화학적 방제에 의존하므로 약제저항성 증가가 야기되므로, 트랩식물을 이용한 대체방제법 개발을 위해 허브식물들에 대한 담배가루이의 기주 선호성을 조사하였다. 완주군에 있는 국립원예특작과학원 내 토마토온실에서 실험을 진행하였으며, 허브 식물로 레몬밤, 초코민트, 로즈제라늄, 애플제라늄을 지면으로 부터 50 cm와 작물 상단부의 30 cm 위치에 각각 설치하여 담배가루이 밀도를 조사하였다. 허브식물의 설치 위치별 담배가루이 밀도를 각각 조사한 결과 레몬밤이 설치된 곳에서 높았으며 로즈제라늄과 애플제라늄은 오히려 기피하는 것으로 나타났다. 이 결과는 유인제, 기피제 및 트랩식물을 포함한 허브 식물을 사용하는 담배가루이의 대체방제법 개발에 도움이 될 것으로 기대된다. The problem of increased resistance to pesticides is caused by the management of Bemisia tabaci, which depends almost entirely on pesticides, and to solve this problem, we investigated the host preference of B. tabaci among herbal plants to develop a possible attractant for alternative control methods. Experiments were conducted in greenhouse tomatoes at the National Institute of Horticultural Science and Herbal Science in Wanju-gun. Major herbal plants such as lemon balm, chocolate mint, rose geranium, and apple geranium were installed 50cm from the ground and 30cm from the top of the tomatoes, then the density of B. tabaci was investigated. As a result of examining the density of B. tabaci by the location of the herbal plants, it was found that the lemon balm location showed the highest B. tabaci density while rose geranium and apple geranium were rather avoided. These results are expected to be helpful in the development of alternative B. tabaci control methods using herbal plants including attractants, repellents, and trap plants.

블루베리와 복숭아에서 전정가지 수분함량이 갈색날개매미충 알 부화에 미치는 영향

김동환,양창열,김형환,서미혜,윤정범,Kim, Dong Hwan,Yang, Chang Yeol,Kim, Hyeong Hwan,Seo, Mi Hye,Yoon, Jung Beom 한국응용곤충학회 2017 한국응용곤충학회지 Vol.56 No.4

12월부터 3월까지 갈색날개매미충 알이 붙어 있는 블루베리나무와 복숭아나무의 가지를 전정한 후 가지의 마름정도와 갈색날개매미충 알의 부화율을 조사하였다. 갈색날개매미충 알의 부화율은 블루베리와 복숭아 모두 1% 미만으로 정상적인 가지(52.8~68.4%)에 비해 부화율이 매우 낮은 수준이었다. 부화시기에 전정가지의 함수율은 블루베리와 복숭아 각각 9.8~20.9%, 7.5~13.8%로 매우 낮았다. 즉 갈색날개매미충의 알이 붙어 있는 가지를 전정할 경우 가지가 심하게 마르기 때문에 가지 속의 알도 건조해져서 정상적으로 부화하지 못하게 되는 것으로 나타났다. Hatchability of eggs and rate of weight reduction in pruned twigs with egg-mass of R. shantungensis were investigated in blueberry and peach orchards from December to March. Hatchability of R. shantungensis eggs were 52.8~68.4% on normal twigs in the field. However, the rate of hatchability dropped to less than 1% after pruning. Moisture content of twigs after pruning blueberry and peach were 9.8~20.9% and 7.5~13.8%, respectively. This result revealed that the hatchability of R. shantungensis eggs might be very low or nil, because the eggs dried up after pruning of the twigs with egg-masses of R. shantungensis.

환경제어 조건에서 방충망 색과 크기가 담배가루이 및 꽃노랑총채벌레의 물리적 방제에 미치는 영향

정충렬 ( Chung Ryul Jung ),윤정범 ( Jung Beom Yoon ),김광호 ( Kwang Ho Kim ),이광재 ( Guang Jae Lee ),허정욱 ( Jeong Wook Heo ),김현환 ( Hyun Hwan Kim ) 한국환경농학회 2016 한국환경농학회지 Vol.35 No.1

BACKGROUND: The tobacco whitefly(Bemisia tabaci Gennadius) and western flower thrips(Frankliniella occidentalis Pergande) seriously damaged to several greenhouse crops and transmitted plant viruses such as the Tomato Yellow Leaf Curl Virus(TYLCV) and Tomato Spotted Wilt Virus(TSWV). Objective of this study was to elucidate exclusion effects of insect screen nets by various hole sizes and colors for control of the two insect pests in controlled environments such as a closed plant production system. METHODS AND RESULTS: The exclusion effects to various hole sizes of three other colors with 30 individuals of two insect pests was evaluated. B. tabaci was not showed not difference to different colors and sizes. F. occidentalis showed that 0.2 mm black screen was the most effective exclusion than other colors of 0.6 and 0.8 mm. CONCLUSION: The two insects were different reponses to various hole sizes of white and other color screen nets. It was proved that the 0.4 mm white screen net used in this experimental condition was suitable for exclusion of B. tabaci and 0.2 mm black for F. occidentalis.