http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
오유경(Yu Kyoung Oh),손태종(Tae Jong Son),신광숙(Kwang Sook Sin),강민정(Min Jeong Kang),김정목(Jung Mogg Kim),김남근(Nam Keun Kim),고정재(Jung Jae Ko),김종국(Chong Kook Kim) 한국약제학회 2001 Journal of Pharmaceutical Investigation Vol.31 No.3
N/A Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the viruslike particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.
연구논문 : 생명과학 ; 폴록사머 및 프로필렌글리콜을 이용한 클로트리마졸 고형 좌제의 물리화학적 특성
현경희 ( Jing Ji Xuan ),오유경 ( Yu Kyoung Oh ),김정애 ( Jung Ae Kim ),공경환 ( Kyung Hwan Gong ),김지현 ( Ji Hyun Kim ),양준호 ( Joon Ho Yang ),배명수 ( Myung Soo Bae ),김호동 ( Ho Dong Kim ),이종달 ( Jong Dal Rhee ),장현욱 ( Hye 영남대학교 약품개발연구소 2005 영남대학교 약품개발연구소 연구업적집 Vol.15 No.-
연구논문 : 생명과학 ; 인간에서의 경구흡수를 촉진하는 투명 아세클로페낙 연질캅셀의 개발
용철순 ( Chul Soon Yong ),오유경 ( Yu Kyoung Oh ),이경희 ( Kyung Hee Lee ),박상만 ( Sang Man Park ),길영식 ( Young Sig Gil ),유창훈 ( Chang Hun Yu ),김종오 ( Jong Oh Kim ),유봉규 ( Bong Kyu Yoo ),이종달 ( Jong Dal Rhee ),김종국 ( 영남대학교 약품개발연구소 2005 영남대학교 약품개발연구소 연구업적집 Vol.15 No.-