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Animal models for dengue vaccine development and testing
나운성,염민주,최일규,육희준,송대섭 대한백신학회 2017 Clinical and Experimental Vaccine Research Vol.6 No.2
Dengue fever is a tropical endemic disease; however, because of climate change, it may become a problem in South Korea in the near future. Research on vaccines for dengue fever and outbreak preparedness are currently insufficient. In addition, because there are no appropriate animal models, controversial results from vaccine efficacy assessments and clinical trials have been reported. Therefore, to study the mechanism of dengue fever and test the immunogenicity of vaccines, an appropriate animal model is urgently needed. In addition to mouse models, more suitable models using animals that can be humanized will need to be constructed. In this report, we look at the current status of model animal construction and discuss which models require further development.
Influenza virus vaccine for neglected hosts: horses and dogs
나운성,염민주,육희준,문형준,강보규,송대섭 대한백신학회 2016 Clinical and Experimental Vaccine Research Vol.5 No.2
This study provides information regarding vaccine research and the epidemiology of influenza virus in neglected hosts (horses and dogs). Equine influenza virus (EIV) causes a highly contagious disease in horses and other equids, and outbreaks have occurred worldwide. EIV has resulted in costly damage to the horse industry and has the ability of cross the host species barrier from horses to dogs. Canine influenza is a virus of equine or avian origin and infects companion animals that live in close contact with humans; this results in possible exposure to the seasonal epizootic influenza virus. There have been case reports of genetic reassortment between human and canine influenza viruses, which results in high virulence and the ability of transmission to ferrets. This emphasizes the need for vaccine research on neglected hosts to update knowledge on current strains and to advance technology for controlling influenza outbreaks for public health.
Sputum Processing Method for Lateral Flow Immunochromatographic Assays to Detect Coronaviruses
강아람,염민주,김혜권,윤선우,정대균,문형준,류광수,나운성,송대섭 대한면역학회 2021 Immune Network Vol.21 No.1
Coronavirus causes an infectious disease in various species and crosses the species barriers leading to the outbreak of zoonotic diseases. Due to the respiratory diseases are mainly caused in humans and viruses are replicated and excreted through the respiratory tract, the nasal fluid and sputum are mainly used for diagnosis. Early diagnosis of coronavirus plays an important role in preventing its spread and is essential for quarantine policies. For rapid decision and prompt triage of infected host, the immunochromatographic assay (ICA) has been widely used for point of care testing. However, when the ICA is applied to an expectorated sputum in which antigens are present, the viscosity of sputum interferes with the migration of the antigens on the test strip. To overcome this limitation, it is necessary to use a mucolytic agent without affecting the antigens. In this study, we combined known mucolytic agents to lower the viscosity of sputum and applied that to alpha and beta coronavirus, porcine epidemic diarrhea virus (PEDV) and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, spiked in sputum to find optimal pretreatment conditions. The pretreatment method using tris(2-carboxyethyl)phosphine (TCEP) and BSA was suitable for ICA diagnosis of sputum samples spiked with PEDV and MERS-CoV. This sensitive assay for the detection of coronavirus in sputum provides an useful information for the diagnosis of pathogen in low respiratory tract.
문형준,오진식,나운성,염민주,한상윤,김성재,박봉균,송대섭,강보규 대한면역학회 2016 Immune Network Vol.16 No.5
A pandemic influenza A (H1N1) virus strain was isolated from a pig farm in Korea in December 2009. The strain was propagated in and isolated from both the Madin-Darby canine kidney cell line and embryonated eggs. The partial and complete sequences of the strain were identical to those of A/California/04/2009, with >99% sequence similarity in the HA, NA, M, NS, NP, PA, PB1, and PB2 genes. The isolated strain was inactivated and used to prepare a swine influenza vaccine. This trial vaccine, containing the new isolate that has high sequence similarity with the pandemic influenza A (H1N1) virus, resulted in seroconversion in Guinea pigs and piglets. This strain could therefore be a potential vaccine candidate for swine influenza control in commercial farms.
박근선,임종우,박채원,염민주,함승주 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Avian influenza virus (AIV) causes acute infectious diseases of poultry, especially High pathogenic AIV (HPAIV) are cause of morbidity and mortality. Therefore, it is very important to take preventive measures through rapid detection of HPAIV for minimizing economic loss. In this study, we developed cell mimicking nanoparticles (CMPs) for a rapid detection of HPAIV and LPAIV via viral fusion mechanism. CMPs was polymeric nanoparticles constructed with sialic acid and FRET dye pairs, exposing FRET off signal responding to LPAIV and HPAIV activated by enzymatic cleavage at endosomal condition. CMPs exhibited accurate detection of various types of LPAIVs and HPAIVs in both cases with egg-cultured and fecal spiked specimen, showing superior sensitivity compared to rapid kit. The proposed strategy for mimicking viral infection can be a potent diagnostic assay for LPAIV and HPAIV, contributing to minimize economic loss by execution of preventative measures against the viral outbreaks.
박주원,김진영,함승주,박채원,김은정,염민주 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Viral nucleic acids are a valuable source for disease detection since it encodes every characteristic of a virus. This study presents a system in which flap endonuclease 1 (FEN1) is used to spot and quantify viral cDNA in a sensitive and swift manner. FEN1 is known to remove 5’ overhanging DNA flaps depending on specific double-flap structures. Synthetic DNA strands according to random cDNA fragments of viral mRNA target sequence forms a double-flap structure with two kinds of additive oligonucleotides, enabling recognition by FEN1: Raman dye labeled probe that is partially complementary and forms a 5’-flap, and another sequence providing 3’-1nt flap. FEN1 removes the Raman dye labeled 5’-flap from the double-flap substrate which is used to trace the presence of target viral gene sequence by SERS. This system detecting any gene with considerably high speed and sensitivity can lead to a step forward to preemptive prevention of widespread viral infectious diseases.