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CCR7 Ligand의 Memory CD4+ T 세포 증가유도 및 바이러스 감염에 대한 방어효과
어성국,조정곤,Eo, Seong-Kug,Cho, Jeong-Gon 대한면역학회 2003 Immune Network Vol.3 No.1
Background: CC chemokine receptor (CCR) 7 and cognate CCR7 ligands, CCL21 (formerly secondary lymphoid tissue chemokine [SLC]) and CCL19 (formerly Epstein-Barr virus-induced molecule 1 ligand chemokine [ELC]), were known to establish microenvironment for the initiation of immune responses in secondary lymphoid tissue. As described previously, coadministration of DNA vaccine with CCR7 ligand-encoding plasmid DNA elicited enhanced humoral and cellular immunity via increasing the number of dendritic cells (DC) in secondary lymphoid tissue. The author hypothesized here that CCR7 ligand DNA could effectively expand memory CD4+ T cells to protect from viral infection likely via increasing DC number. Methods: To evaluate the effect of CCR7 ligand DNA on the expansion of memory CD4+ T cells, DO11.10.BALB/c transgenic (Tg)-mice, which have highly frequent ovalbumin $(OVA)_{323-339}$ peptide-specific CD4+ T cells, were used. Tg-mice were previously injected with CCR7 ligand DNA, then immunized with $OVA_{323-339}$ peptide plus complete Freund's adjuvant. Subsequently, memory CD4+ T cells in peripheral blood lymphocytes (PBL) were analyzed by FACS analysis for memory phenotype ($CD44^{high}$ and CD62 $L^{low}$) at memory stage. Memory CD4+ T cells recruited into inflammatory site induced with OVA-expressing virus were also analyzed. Finally, the protective efficacy against viral infection was evaluated. Results: CCR7 ligand DNA-treated Tg-mice showed more expanded $CD44^{high}$ memory CD4+ T cells in PBL than control vector-treated animals. The increased number of memory CD4+ T cells recruited into inflammatory site was also observed in CCR7 ligand DNA-treated Tg-mice. Such effectively expanded memory CD4+ T cell population increased the protective immunity against virulent viral infection. Conclusion: These results document that CCR7 and its cognate ligands play an important role in intracellular infection through establishing optimal memory T cell. Moreover, CCR7 ligand could be useful as modulator in DNA vaccination against viral infection as well as cancer.
천연 퀘루세틴이 수종 항생물질의 항균력에 미치는 병용효과
어성국,김영소,이종길,이도익,김일혁,한성순,Eo, Seong-Kug,Kim, Young-So,Lee, Chong-Kil,Lee, Do-Ik,Kim, Il-Hyuk,Han, Seong-Sun 대한약학회 1996 약학회지 Vol.40 No.6
As part of our search for less toxic antimicrobial agents from natural resources. rutin was isolated from Sophora japonica and then hydrolyzed to quercetin. Antimicrobial activity of quercetin was tested in vitro against five kinds of gram positive and ten kinds of gram negative bacteria by serial broth dilution method. Among fifteen kinds of bacteria tested, the antimicrobial activity of quercetin was the most potent against Proteus vulgaris showing minimal inhibitory concentration(MIC) of 125 ${\mu}$g/ml. To investigate the effect of antimicrobial combinations of quercetin with four kinds of antibiotics (ampicillin, cefazolin, oxytetracycline and chloramphenicol). the fractional inhibitory concentration index (FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of quercetin with four kinds of antibiotics resulted in synergism in one instance, additive effect in four instances, but no antagonism was observed.
천연 Hesperetin의 항허피스바이러스작용과 Acyclovir 및 Vidarabine과의 병용효과
이지현,어성국,김영소,이종길,한성순,Lee, Ji-Hyun,Eo, Seong-Kug,Kim, Young-So,Lee, Chong-Kil,Han, Seong-Sun 한국생약학회 1999 생약학회지 Vol.30 No.1
To search for less toxic antiherpetic agents, the inhibitory effects of natural hesperetin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by the plaque reduction assay in vitro. Hesperetin inhibited plaque formations of HSV-1 and HSV-2 in a dose dependent manner. It also exhibited more potent antiherpetic activity on HSV-2 with selectivity index (SI) of 9.8 than on HSV-1 with SI of 8.4. The combined antiherpetic effects of hesperetin with nucleoside antiherpetic agents, acyclovir and vidarabine, were examined on the multiplication of these two strains of herpesviruses in Vero cells by the combination assay. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combinations of hesperetin with acyclovir on HSV-1 and HSV-2 showed synergistic effects with CI values of $0.29{\sim}0.73$ for 50%, 70%, 90% effective levels and those with vidarabine showed partially synergistic or additive effects with CI values of $0.83{\sim}1.33$.
임교환,어성국,김영소,이종길,한성순,Rym, Kyo-Hwan,Eo, Seong-Kug,Kim, Young-So,Lee, Chong-Kil,Han, Seong-Sun 한국생약학회 1996 생약학회지 Vol.27 No.4
As part of our search for less toxic antimicrobial agents from natural resources, antimicrobial activity of rutin isolated from Sophora japonica was tested in vitro against four kinds of gram positive bacteria and four kinds of gram negative bacteria by serial broth dilution method. Among eight kinds of bacteria tested, the antimicrobial activity of rutin was the most potent against Mycobacterium smegmatis showing MIC of $375\;{\mu}g/ml$. The acute toxicity of rutin was examined in mice and rats. and $LD_{50}$ value administered intraperitoneally in mice was 650 mg/kg(Confidence limit: 894-1,473 mg/kg). There were no significant changes in serum biochemical values and histopathological changes as compared with those of control after intraperitoneal administration with rutin in rats.
잔나비걸상 수용성 물질의 Vesicular Stomatitis Virus (Indiana Serotype)에 대한 항바이러스작용과 Interferon과의 병용효과
김영소,어성국,한성순,Kim, Young-So,Eo, Seong-Kug,Han, Seong-Sun 한국생약학회 1999 생약학회지 Vol.30 No.3
In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [Indiana serotype, VSV(IND)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or gamma were examined on the multiplication of VSV(IND). EA caused a concentration-dependent reduction in the plaque formation of VSV(IND) with 50% effective concentration $(EC_{50})$ of $104.02\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. All cases of the combination of EA with IFN alpha or IFN gamma showed potent synergism with CI values of $0.38{\sim}0.52$ for $50{\sim}90%$ effective levels.
임교환,어성국,김영소,이종길,한성순,Rym, Kyo-Hwan,Eo, Seong-Kug,Kim, Young-So,Lee, Chong-Kil,Han, Seong-Sun 한국생약학회 1999 생약학회지 Vol.30 No.1
In order to find less toxic antiviral agents from basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. EA was examined for antiviral activity against five strains of pathogenic viruses such as encephalomyocarditis virus (EMCV), vesicular stomatitis virus (VSV) Indiana and New Jersey strains, influenza A virus (Flu A), and varicella zoster virus (VZV) in vitro. Antiviral activity was evaluated by plaque reduction assay. Among five strains of viruses tested, EA exhibited the most potent antiviral activity against VSV Indiana strain with 50% effective concentration $(EC_{50})$ of 0.104 mg/ml in Vero cells, and its selectivity index (SI) was 36.5. EA was also examined for the virucidal activity, antiviral activity in preincubation on VSV Indiana strain in order to examine possible mode of antiviral activity. Preincubation of Vero cells with EA did not confer protection against VSV, however, prolonged exposure of cells to EA inhibited the replication of virus dose-dependently. In virucidal activity, the titer of infectious virus did not decrease significantly.
잔나비걸상버섯 수용성물질의 항인플루엔자바이러스 작용과 인터페론과의 병용효과
정선식(Sun Sik Jeong),어성국(Seong Kug Eo),김영소(Young So Kim),한성순(Seong Sun Han) 대한약학회 1999 약학회지 Vol.43 No.4
EA. the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Anti-influenza A virus (anti-Flu A) activity of EA was examined in Vero cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha and gamma were examined on the multiplication of Flu A. EA showed a concentration-dependent reduction in the plaque formation of Flu A with 50% effective concentration (EC50) of 1.50mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha on Flu A showed more potent synergism with CI values of 0.50~0.52 of 50%, 70%, 90% effective levels than that with IFN gamma with CI values of 0.82~0.99.