기피-유인을 이용한 바퀴의 효율적 방제

양정오(Jeong-Oh Yang),김상우(Sang-Woo Kim),노두진(Doo-Jin Noh),윤창만(Changmann Yoon),강신호(Shin-Ho Kang),김길하(Gil-Hah Kim) 한국농약과학회 2008 농약과학회지 Vol.12 No.2

To enhance the control efficacy against cockroaches in dwelling space, repellent (“Push”) was set down on one comer and a poison bait including attractant (“Pull”) was on the opposite side. And we tested the control efficacy of Push-Pull strategy by behavior-stimuli of cockroaches. In a mini-field test, German cockroach males primarily chose shelters nearest to the attractant-treated surfaces and farthest from the repellent-treated surfaces. The consumption of food or bait was also highest from food nearest to the preferred shelters by push-pull effect. We evaluated the push-pull insecticidal efficacies of five essential oils belong to Citrus plant, namely, grapefruit, lemon, lime, orange, and petitgrain. The combined push-pull treatments appeared to be faster and higher insecticidal effects than single (pull) treatment, and the repellent efficacy of Citrus oils was in the order of grapefruit > lemon > lime > orange > petitgrain. Therefore, we propose the effective control of the german cockroach using Citrus oils as repellents and push-pull method into a limited area.

고추와 토마토 품종별 담배가루이 B와 Q biotype의 섭식행동 비교

양정오,김은희,윤창만,안기수,김길하,Yang, Jeong-Oh,Kim, Eun-Hee,Yoon, Chang-Mann,Ahn, Ki-Su,Kim, Gil-Hah 한국응용곤충학회 2009 한국응용곤충학회지 Vol.48 No.2

담배가루이 B와 Q biotype은 토마토 모든 7개 시험품종에서 정상적인 발육을 하였으나 고추 품종에서의 발육은 다르게 나타났다. B biotype은 9개 고추 품종에서 모두 발육이 정상적으로 이루어지지 않았으나 Q biotype은 정상적으로 발육하였다. 그러나 Q biotype은 녹광 품종에서는 낮은 우화율을 보였다. EPG를 이용한 체관부 섭식시간 분석에서 B와 Q biotype은 고추 품종에 대한 체관부 섭식시간에 차이가 있었으며 Q가 B biotype보다 섭식시간이 길었다. 고추 품종에서 Q biotype은 녹광 품종에서 짧은 섭식시간을 보여주어 녹광 품종을 섭식하지 않는 것으로 나타났으나, 나머지 고추 8개 품종에서는 섭식시간에 차이가 없었다. 또한, B biotype은 고추를 섭식하지 않는 것으로 나타났다. 토마토 품종에 대한 두 biotype의 체관부 섭식시간에는 차이가 없었다. Nymphal development of the B and Q biotypes of Bemisia tabaci was normal on all seven tomato varieties tested. However, their nymphal development was different on red pepper varieties. B biotype was not normally developed on nine red pepper varieties tested. On the contrary, Q biotype was normally developed, but its adult emergence rate was very low in Nokkwang variety than in other eight varieties. The EPG analysis of the feeding behavior of Bemisia tabaci showed that B and Q biotypes had different duration of phloem phases on red pepper. Q biotype showed longer phloem phases than B biotype. On Nokkwang variety, Q biotype had short phloem phases and did not prefer to feed on Nokkwang variety. Interestingly, Q biotype was found to have long duration of phloem phases on eight red pepper varieties, but B biotype did not prefer to feed on red pepper varieties. However, both biotypes did not show any difference in feeding time on tomato varieties.

Fenobucarb와 Imidacloprid의 벼 뿌리 침지시간에 따른 벼멸구의 살충활성과 섭식행동비교

양정오(Jeong-Oh Yang),조선란(Sun-Ran Cho),권윤희(Yun-Hee Kwon),윤창만(Changmann Yoon),김길하(Gil Hah Kim) 한국농약과학회 2010 농약과학회지 Vol.14 No.2

Two insecticides, fenobucarb (36, 12 ppm) and imidacloprid (0.7, 0.4 ppm) were treated on rice seedling roots by root uptake method with different dipping time (1, 4, 8, 12, 16, 20, 24 hrs). This study was performed to elucidate the correlation between insecticidal activity and feeding behavior of Nilaparvata lugens using EPG (electrical penetration graph). EPG waveforms are recorded for 4 hours and classified into six waveforms. In the correlation between root uptake and corrected mortality, both insecticides showed higher corrected mortality as higher doses and as longer root uptake times. In the analysis of waveforms, N. lugens showed longer nonprobe time but shorter phloem feeding time at a higher dose. It was also showed the same result as longer the root uptake times. Therefore, it showed the correlation between insecticidal activity by root uptake time and both EPG waveforms (non-probing time and phloem feeding time).

3종의 살충제에 대한 담배가루이 Biotype B와 Q의 섭식행동 비교

서미자,양정오,윤창만,윤영남,김길하,Seo, Mi-Ja,Yang, Jeong-Oh,Yoon, Chang-Mann,Youn, Young-Nam,Kim, Gil-Hah 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.3

Acetamiprid, spinosad, thiamethoxam 3종류의 살충제에 대해 담배가루이 biotype B와 Q 타입간의 섭식행동을 EPG기법으로 분석하였다. 3종류의 살충제를 추천농도로 처리한 후 6시간동안 기록한 EPG waveform을 가지고 약제에 반응하여 최초로 구침을 빼기까지 소요된 시간, 전체 기록시간동안의 구침을 빼고 있는 시간, 구침활성이 일어나는 시간, 직접적인 섭식행동인 체관부 섭식시간 등을 조사, 비교분석하여 타입별 살충제에 대한 영향을 평가하였다. 살충제간 약간의 차이를 보였으나, 3가지 살충제 모두 기록 시작 후 살충제에 반응하여 구침의 활성에 변화를 보이는 전체시간을 측정한 결과, B biotype이 Q biotype에 비해 구침의 활성시간이 높게 나타났으며, 체관부를 흡즙하는 시간은 반대로 적게 나타났다. 살충제에 반응하여 구침을 빼기까지 소요된 시간은 B타입이 Q타입보다 빨리 나타났는데, 이는 살충제에 좀 더 빠르게 반응한 것으로 보인다. 결과적으로 담배가루이 biotype B는 Q타입보다 식물체에 처리된 살충제를 빠르게 감지하며 섭식을 덜 함으로써 3가지 살충제에 대해 감수성 경향을 보이는 것으로 확인되었다. The feeding behaviors of 2 biotypes (type B and Q) of tobacco whitefly, Bemisia tobaci, were monitored using EPG technique on tomato and pepper plants treated 3 insecticides for controlling whiteflies, for examples, acetamiprid, spinosad and thiamethoxam. After treatment of three insecticides with recommended concentrations to tomato and pepper plants, EPG waveforms were recorded during 6 hours. The characteristic patterns of feeding behaviors investigated were as follows; time consumed by withdrawal of proboscis, total non-penetration time, total stylet pathway pattern time and total phloem feeding time. There was somewhat difference among 3 insecticides tested. As a result of investigation of total duration showed the stylet pathway activity due to the reaction against all tested insecticides, the Q biotype showed fewer time than those from the B biotype. The B biotype showed more frequent stylet pathway activity patterns during whole recording time and a shorter phloem ingestion time than those from the Q biotype. In result of prior up (non-penetration) time representing the reaction against the insecticide treated, the time of B biotype was more faster than that of the Q biotype, so it was considered that the B biotype was more sensitive to the tested insecticides. Therefore, our results revealed a clear difference in feeding behaviour between the B and Q biotypes of B. tabaci. Also, it was investigated that B biotype was susceptible to the 3 insecticides.

전자빔 조사가 담배가루이, 복숭아혹진딧물, 배추좀나방과 점박이응애의 발육과 생식에 미치는 영향

문상래,손봉기,양정오,우종석,윤창만,김길하,Moon, Sang-Rae,Son, Bong-Ki,Yang, Jeong-Oh,Woo, Jong-Suk,Yoon, Chang-Mann,Kim, Gil-Hah 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.2

전자빔 조사(30, 50, 70, 100 Gy)가 주요 농업해충(담배가루이, 복숭아혹진딧물, 배추좀나방, 점박이응애)의 발육과 생식에 미치는 영향을 조사하였다. 담배가루이, 배추좀나방, 점박이응애 알의 부화는 조사량이 많아질수록 억제되었으며, 특히 담배가루이와 점박이응애 알은 10 0Gy 조사량에서 부화가 100%억제되었다. 그러나 약충/유충, 번데기, 담배가루이, 배추좀나방, 점박이응애의 알에 조사했을 때 부화유충의 우화억제에 영향을 미쳤다. 그리고 70 Gy 조사량에서 담배가루이 우화성충은 산란을 하지 않았고, 100 Gy 조사량에서 배추좀나방 우화성충의 산란수가 감소하였다. 담배가루이, 배추좀나방, 복숭아혹진딧물, 점박이응애 약충/유충에 조사했을 때 70과 100 Gy 조사량에서 부화유충에 미치는 영향과 비슷하였다. 배추좀나방 번데기에 조사했을 때 100 Gy에서 산란수가 감소하였고, 산란한 알이 100% 부화하지 못했다. 담배가루이, 배추좀나방, 복숭아혹진딧물, 점박이응애의 성충에 조사했을 때 70, 100 Gy에서 산란수/산자수가 감소하였으며, 산란한 알의 부화억제에도 영향을 미쳤다. 특히 담배가루이 알은 부화가 100%억제되었다. 그러나, 4종의 주요 농업해충의 모든 발육태에 조사하였을 때 성충수명에는 영향이 없었다. Four major agricultural insect pests, Bemisia tabaci, Myzus persicae, Plutella xylostella and Tetranychus urticae, were irradiated with 30, 50, 70, or 100 Gy electron-beam. Longevity, egg hatching, emergence, and fecundity of the test insects were measured. Hatchability of B. tabaci, P. xylostella and T. urticae declined with increasing irradiation doses, and all B. tabaci and T. urticae eggs were dead at 100 Gy. When eggs of B. tabaci, P. xylostella and T. urticae were irradiated, hatch was inhibited. B. tabaci adults grown from 70 Gy irradiated eggs did not lay eggs. Fecundity of P. xylostella from the 100 Gy irradiated eggs decreased. When B. tabaci, P. xylostella, M. persicae and T. urticae nymphs/larvae were irradiated, the results were similar as those of the hatched eggs. When P. xylostella pupae were irradiated with 100 Gy, fecundity of emerged adults decreased and no eggs hatched. When B. tabaci, P. xylostella, M. persicae and T. urticae adults were irradiated with 70 and 100 Gy, fecundity decreased and egg hatch of B. tabaci was inhibited. However, the longevity of adults did not change and electron-beam irradiation of all developmental stages had no effect on the longevity of adults.

갈색여치의 발생소장 및 발육특성

문상래,노두진,양정오,윤창만,안기수,김길하,Moon, Sang-Rae,Noh, Doo-Jin,Yang, Jeong-Oh,Yoon, Chang-Mann,Ahn, Ki-Su,Kim, Gil-Hah 한국응용곤충학회 2009 한국응용곤충학회지 Vol.48 No.1

충북 영동지역 과수원에 대발생하여 피해를 주고 있는 갈색여치의 발생소장, 영기별 발육특성, 온도별 발육기간, 수명 및 산란수를 조사하였다. 갈색여치는 2008년 3월 말부터 9월 초까지 발생하며 5월 중순이 발생최성기였다. 3월부터 부화약충이 나타나기 시작하여 과수를 가해하기 시작하는데 피해가 가장 많은 시기는 4-5령 약충인 5월 중순이며, 성충은 6월 상순부터 8월 중순까지 나타나며 발생최성기는 7월 중순이었다. 갈색여치의 전체밀도는 5월 중순이 가장 높았다. 1령에서 7령 약충기를 거쳐 성충이 되는 갈색여치는 4령부터 산란관이 바깥으로 노출되고 체중이 크게 증가하며, 6령부터 날개가 관찰되었다. 채집된 개체의 성비는 0.57로 암컷이 많았다. 영기별 약충 발육기간은 영기가 높아질수록 길어졌고, 20, 25, $30^{\circ}C$의 항온조건에서는 온도가 높아질수록 짧았다. 20, 25, $30^{\circ}C$순으로 온도가 높아질수록 산란전기간 역시 각각 5.0, 4.3일 그리고 3.4일로 짧아지고, 산란수는 각각 69.0, 87.1개 그리고 104.3개로 증가하였으나 성충수명은 $25^{\circ}C$조건에서 암수 각각 35.7, 32.9일로 가장 길고, $30^{\circ}C$에서 각각 30.1, 28.1일로 가장 짧았다. 암수 간 발육기간의 차이는 온도와 관계없이 암컷이 더 길었다. 산란은 토양 표면으로부터 $3{\sim}4\;cm$깊이에 가장 많이 분포하였으며, 산란초기에 알을 집중적으로 낳는 습성을 보였다. This study was performed to investigate the seasonal occurrence, developmental characteristics of each nymphal stages with different temperatures (20, 25, $30^{\circ}C$), longevity and fecundity of ussur brown katydid, Paratlanticus ussuriensis, damaging by outbreaks in the orchard areas of Bitan-ri, Yeongdong, Chungbuk. Paratlanticus ussuriensis occurred from late-March to late-August with peak of mid-May. Newly emerged nymphs appeared from March and do damaged fruit orchards with peak of mid-May when P. ussuriensis existed as 4th and 5th nymphal stages. P. ussuriensis adult occurred from early-June to mid-Aug. with peak of mid-July. Total density of P. ussuriensis was showed highest in mid-May. Paratlanticus ussuriensis goes through nymphal stages to 7th nymph, the ovipositor began exposed to outside from the 4th instar and the body weight increased heavily from this stage and the wings were observed from 6th instar. Developmental period was longer as increased the nymphal stages. Sex ratio of collected insect was showed as 0.57; females more than males. As increased the temperature, developmental period was to be short. Preoviposition was also to be short as 5.0, 4.3, and 3.4 days at 20, 25, $30^{\circ}C$, respectively, and fecundity increased as 69.0, 87.1, and 104.3 at 20, 25, $30^{\circ}C$, respectively. Longevity of male and female at $25^{\circ}C$ was showed the longest with 35.7, and 32.9 days and showed the shortest with 30.1 and 28.1 days at $30^{\circ}C$, respectively. The difference of developmental period in male and female were showed longer in female without relation of temperature. The eggs laid were frequently distributed 3 to 4 cm from soil surface, and showed the behavior laying eggs intensively when early oviposition period.

갈색여치의 유인물질 탐색과 트랩개발

노두진(Doo-Jin Noh),양정오(Jeong-Oh Yang),문상래(Sang-Rae Moon),윤창만(Changmann Yoon),강신호(Changmann Yoon),안기수(Ki-Su Ahn),김길하(Gil-Hah Kim) 한국농약과학회 2008 농약과학회지 Vol.12 No.3

Recently, Ussur brown katydid outbreaks caused a serious pest problem in areas of Yeongdong, Chungbuk. This study was performed to control the pest with environment-friendly method. Trap was made of PET plastic bottles that easily found near farmhouse. Attractant materials such as oak (Quercus acutissima) leaf, fruits (peach, apple, grapefruit and pear) sarcocarp or its juices, rice wine (makgeolli) and fish meal were directly applied into the manufactured trap and investigated for the attraction efficacy compared with the funnel trap. During one day, manufactured trap (fish trap) attracted the Ussur brown katydid more than funnel trap. The efficacy of attractant materials were as follows: peach juice (32.7 adults) > rice wine+fish meal (31.3 adults) > rice wine (27.0 adults) > pear juice (19.0 adults) > apple juice (17.2 adults) > fish meal (16.7 adults) > grapefruit juice (14.4 adults) > oak leaf (2.3 adults). The attractive efficacy of fruit juices to ussur brown katydid was more than fruit carcocarps, and the trap hangover 1m in height more than that on ground, The composition of rice wine and fish meal prolonged its efficacy when treated with disinfectant.

꽃매미(Lycorma delicatula)의 발육단계별 표피탄화수소 비교

조선란,이정은,정진원,양정오,윤창만,김길하,Cho, Sun-Ran,Lee, Jeong-Eun,Jeong, Jin-Won,Yang, Jeong-Oh,Yoon, Chang-Mann,Kim, Gil-Hah 한국응용곤충학회 2011 한국응용곤충학회지 Vol.50 No.3

Aliphatic cuticular hydrocarbons (CHCs) of different developmental stages of the spot clothing wax cicada, Lycorma delicatula (Hemiptera: Fulgoridae) were analyzed using GC and GC-MS. The numbers of carbons in the major CHCs of each developmental stage 32, 33, 28, 38, 37 in the egg, 1st, 2nd, 3rd, and 4th instar nymphal stages, and adults, respectively. The cuticle of Lycorma delicatula contains mainly methyl-branched 9-methylheptacosane (15.11%) in the egg stage, and a high proportion of n-heptacosane in nymphal stages (15.75, 22.42, 25.04, and 23.11 % in the 1st, 2nd, 3rd and 4th instars, respectively). In contrast, male and female adults had high proportions of n-nonacosane (13.42 and 16.55%). The chemical constituents of CHCs were classified into five groups (n-alkanes, monomethylalkanes, dimethylalkanes, trimethylalkanes, olefins) and group profiles of each developmental stage were compared. Egg surface was composed mainly monomethylalkanes (45.39%), a saturated hydrocarbon. Nymph CHCs consisted primarily of n-alkanes (37.63 to 46.12%). There was a difference between adult male and female CHCs. However, both contained n-alkanes and monomethylalkanes. CHCs with trimethyl or double bonded structure were rare in all stages. 꽃매미의 발육단계별 표피탄화수소를 비교하기 위하여 GC와 GC-MS를 이용하여 분석하였다. 분석된 꽃매미의 표피탄화수소의 종류는 알이 32종, 약충은 령별로 각각 33, 28, 38, 37종이었고 2령 약충에서 가장 적게 나타났다. 반면 성충은 암 수 모두 46종으로 동일하였으며, 꽃매미의 충태 중 가장 많은 종류의 탄화수소를 함유하고 있었다. 발육단계별 물질 함량을 분석한 결과, 알은 메틸기를 갖고 있는 9-methylheptacosane을 가장 많이 함유하고 있었으며(15.11%), 령기별 약충은 n-heptacosane을 가장 많이 함유하고 있었다(15.75, 22.42, 25.04, 23.11%). 반면 성충은 암 수 모두 n-nonacosane을 가장 많이 함유하고 있었다(13.42, 16.55%). 표피탄화수소의 구성물질을 5개 그룹(n-alkanes, monomethylalkanes, dimethylalkanes, trimethylalkanes, olefins)으로 나누고 물질 함량을 꽃매미 발육단계별로 비교하였다. 꽃매미의 알 표피는 대부분이 monomethylalkanes인 포화탄화수소로 이루어져 있고(45.39%), 약충은 포화탄화수소의 기본구조인 n-alkanes이 대부분이었다(37.63-46.12%). 또한 성충은 암 수에 따라 차이가 있었지만, n-alkanes과 monomethylalkanes을 고루 함유하는 것으로 나타났다. 반면 모든 충태에서, 3개이상의 메틸기를 갖거나 이중 결합을 포함하는 구조는 극히 적었다.

갈색여치에 대한 살충제의 감수성

안기수(Ki-Su Ahn),양정오(Jeong-Oh Yang),노두진(Doo-Jin Noh),윤창만(Changmann Yoon),김길하(Gil-Hah Kim) 한국농약과학회 2007 농약과학회지 Vol.11 No.3

Insecticidal activity of 33 registered insecticides was tested against last nymphal instars and adults of ussur brown katydid (Paratlanticus ussuriensis). All experiments were tested at the recommended concentration of each insecticides by producer. Acephate, chlorpyrifos, diazinon, EPN and fenitrothion which were organophates, and a mixture combined with chlorpyrifos+α-cypemethrin showed 100% mortality of P. ussuriensis. But fipronil showed only 100% mortality in leaf-dipping method. Carbamates insecticidal groups, benfuracarb and furathiocarb were showed over 80% and phenthoate was 60~80% in mortality of P. ussuriensis. Among the mixture, etofenprox+diazinon and esfenvalerate+fenitrothion were showed 60-80% against last nymphal instars of P. ussuriensis. Otherwise, acephate, chlorpyrifos, diazinon, EPN and fenitrothion were showed 100% mortality of P. ussuriensis within only 24 hours after treatment, but there was no effective after then in residual tests with leaves.

Acequinocyl 저항성 점박이응애의 유전과 교차저항성

김은희(Eun-Hee Kim),양정오(Jeong-Oh Yang),윤창만(Changmann Yoon),안기수(Ki-Su Ahn),김길하(Gil-Hah Kim) 한국농약과학회 2007 농약과학회지 Vol.11 No.2

The development of resistance to acequinocyl was found in the population of the twospotted spider mite, Tetranychus urticae, collected from rose greenhouses in Gimhae, Gyeongnam province in January 2001. This pest is reared on 5 years treated with acequinocyl (over 200 times), and increased 87.8 folds in resistance as compared to susceptible strain (S). Inheritance of acequinocyl resistant strain (R) and cross resistance of this strain to 8 acaricides against T. urticae adults and eggs was investigated There were differences of susceptibility in the acequinocyl concentration-mortality relationships in F₁ progenies obtained from reciprocal cross with the S and R strain (S♀×R♂, R♀×S♂). Degrees of dominance were -0.75, -0.57 in F₁ progenies of adult and egg of S♀×R♂. Inheritance in F₁ progenies of S♀×R♂ was incomplete recessive. Degree of dominance were 0.81, 0.45 in F₁ progenies of adult and egg of R♀×S♂, respectively. These results suggest that inheritance of acequinocyl resistance is controlled by a complete dominance. The R strain exhibited cross resistance of 1.1 and 0.9 fold to amitraz, bifenazate, and negatively correlated cross-resistance of 0.08 fold to emamectin benzoate in adult females. The R strain showed cross resistance of 37.7, 14.0, and 26.2 fold to amitraz, milbemectin and spriodiclofen in eggs, respectively. Particularly it showed high levels of cross-resistance to pyridaben with 6538.3 fold. These chemicals showed negatively correlated cross-resistance exhibited 0.4, 0.3, and 0.2 fold to abamectin, bifenazate, and emamectin benzoate in eggs.