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Characterization of a Replication Element in the Coat Protein ORF of Turnip Yellow Mosaic Virus
신현일,조태주 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.1
Turnip yellow mosaic virus (TYMV) is a non-enveloped icosahedral virus that has a single 6.3 kb positive-strand RNA as a genome. Previously, it was observed that the recombinant construct TY-eGFP2, where an eGFP gene was inserted at the position downstream of the coat protein (CP) ORF of TYMV genome, barely replicated. The inhibition of replication was relieved by insertion of an additional copy of the 3' quarter of the CP ORF after the foreign sequence. In this study, we have examined if the 3' quarter of the CP ORF contains any replication elements. M-fold analysis predicted three stem-loop structures in this region. Analysis of the TY-eGFP2 constructs containing one or two of these stem-loop structures indicates that the secondary structure predicted in the region between nt-6139 and nt-6181, termed SL2, is essential for TYMV replication. The critical role of SL2 was confirmed by the observation that deletion of the 3'quarter of the CP ORF from the wild-type TYMV genome nearly abolished replication and that insertion of SL2 into the deletion mutant restored the replication. Mutations disrupting the stem of SL2 greatly reduced viral RNA replication,indicating that the secondary structure is essential for the enhancing activity.
신현일,조태주 대한미생물학회 2013 Journal of Bacteriology and Virology Vol.43 No.1
We have previously observed that a sequence in coat protein (CP) ORF of Turnip yellow mosaic virus (TYMV) isrequired for efficient replication of the virus. The sequence was predicted to take a stem-loop structure, thus termed SL2. While examining various SL2 mutants, we observed that all the modifications resulting in extension of translationbeyond the CP ORF significantly suppressed subgenomic RNA accumulation. The genomic RNA level, in contrast, wasnot affected. Introduction of an in-frame stop codon in the CP ORF of these constructs restored the level of subgenomicRNA. Overall, the results suggest that the read-through makes the subgenomic RNA unstable.
신현일,구창덕,조태주 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3
분석 결과, 하나의 decamer primer 로 모든 품종을 구별할 수는 없었지만 10 종류의 decamer를 사용하면 분석에 사용 한 품종을 모두 구별할 수 있는 것으로 나타났다. 분석한 품종 중 290 과 유우지로 품종의 경우 각각 산지가 다른 두 sample을 분석하였는데, 이 경우에서와 같이 품종은 같으나 산지가 다른 경우에도 약간의 차이가 있는 것으로 나타났다. 이와 같은 결과는 품종 식별에 RAPD 분석방법이 매우 유용하게 사용될 수 있음을 보여주는 것이다. 현재의 RAPD 분석결 과에 근거하면 분석에 사용한 10개 sample 은 다음과 같이 다섯 개 group 으로 나눌 수 있는 것으로 나타났다: Group 1: 옥출, Group 2: 기부, Group 3: 115, Group 4: 602, 대구7, 임협6, Group 5: 290(보은), 290(청원), 유우지로(청원), 유우 지로(괴산).
pH 4.3과 pH 7.0의 과포화용액에서 불소의 농도가 합성 수산화인회석의 결정 성장에 미치는 영향
신현일,박성호,박정원,이찬영 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1
Objectives: Present study was undertaken to investigate the crystal growth onto synthetic hydroxyapatite (HA) seeds in pH 4.3 and pH 7.0 supersaturated solutions with different fluoride concentrations. Materials and Methods: 8 groups of pH 4.3 and 7.0 calcium phosphate supersaturated solutions were prepared with different fluoride concentrations (0, 1, 2 and 4 ppm). Calcium phosphate precipitates yield crystal growth onto the HA seed surface while solutions flow. For evaluation of crystallizing process, the changes of Ca2+, PO43-, F- concentrations of the inlet and outlet solutions were determined. The recovered solid samples were weighed to assess the amount of minerals precipitated, and finally determined their composition to deduce characteristics of crystals. Results: During the seeded crystal growth, there were significantly more consumption of Ca2+, PO43-, F- in pH 4.3 solutions than pH 7.0 (p < 0.05). As fluoride concentration increased in pH 4.3 solution, Ca2+, PO43-, F- consumption in experimental solutions, weight increment of HA seed, and fluoride ratio in crystallized samples were increased. There were significant differences among the groups (p < 0.05). But in pH 7.0 solution, these phenomena were not significant. In pH 7.0 solutions, analyses of crystallized samples showed higher Ca/P ratio in higher fluoride concentration. There were significant differences among the groups (p < 0.05). But in pH 4.3 solution, there were not significant differences in Ca/P ratio. Conclusions: Crystal growth in pH 4.3 solutions was superior to that in pH 7.0 solutions. In pH 4.3 solutions, crystal growth increased with showed in higher fluoride concentration up to 4 ppm.
고장력강판 및 2층아연도금된 고장력 강판의 점용접성에 관한 연구
신현일,강성수 대한용접접합학회 1994 대한용접·접합학회지 Vol.12 No.3
The spot weldability of high strength steel sheet and two stories galvannealed high strength steel sheet has been studied. 1) Tensile shear strength decreased inversely as welding current increased over 12KA in the case of two stories galvannealed high strength steel sheet. 2) When heat flux input over 12KA, hardening region become narrow in case of two stories galvannealed high strength steel sheet. 3) The size of hardening region affect the strength of nuggets.
天主敎 信者에 관한 意識調査 : 대구대교구 봉덕천주교회중심으로 in the case of Catholic Church of Taegu Bongduk
신현일 대구효성카돌릭대학교사회과학연구소 1998 사회과학연구 Vol.5 No.-
This questionnaire survey assessed the belief system and attitudes of the devotees in the Catholic Church Taegu Bongduk This survey was conducted on March 1 in 1997. 987 devotees responded to the questionnaire, but only 882 cases were analysed by the use of SPSS/PC+ program. The items analysed were self-conceit as a devotee, human relations among devotees, offerings, praying at home, and measures of participation in mass, small group activities, missionary activities, etc. The analysis showed as follows; 1. The devotee’s way of thinking about the mass becomes an serious issue. 2. The evaluation to the friendly relations at a parish church was very negative. 3.The degree of participation in piety enhancement activities or group activities at a parish church was also very passive 4. The numbers of family which periodically were praying at home was also very small. 5. The negative attitude to the small piety group revealed itself low participatory level. 6. The self-conceit as a devotee and evaluative attitude to the offerings were generally positive.