http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
연구단보 : 건조 및 저장조건에 따른 쌀 저장단백질의 변화양상
신평균 ( Pyung Gyun Shin ),장안철 ( An Cheol Chang ),홍성창 ( Seong Chang Hong ),이기상 ( Ki Sang Lee ),이금희 ( Keum Hee Lee ),이용복 ( Yong Bok Lee ) 한국환경농학회 2008 한국환경농학회지 Vol.27 No.4
Quality of rice grain changes during dry storage with internal physiological changes and external injury by organism. Storage rice changes by condition with respiration via variable temperature, hydrolysis enzyme reaction, lipid peroxidation occurs with change of palatability. During dry storage, physiological change with protein variation pattern was examined by image analysis on proteomic technology. Analysis revealed that protein activity had no change store at room temperature and store at 40℃, but decreased store at 60℃. Analysis of variable hydrophobic protein pattern revealed that protein activity of beta-tubulin, protein disulfide isomerase, vacuolar ATPase b subunit, globulin was not significantly decreased all dry and store condition. However, heat shock protein 70, and glutathione transferase was significantly decreased when rice dried at 60℃ compared with room temperature and 40℃ dry condition.
수경재배 영양결핍토마토에서 브레시노스테로이드관련 신호전달 단백질 BAK1의 동정
신평균(Pyung-Gyun Shin),장안철(Ancheol Chang),홍성창(Sung-Chang Hong),이기상(Ki-Sang Lee) 한국생명과학회 2007 생명과학회지 Vol.17 No.12
BAK1(Brassinolide insensitive associated receptor kinase 1)는 브라시노스테로이드 생합성 대사관련 신호전달 매체이다. BR 생합성 및 신호전달 돌연변이체는 매우 특징적인 난쟁이 표현형을 보인다. 과채류전용 양액배지인 Sonneveld 양액을 이용하여 양분결핍에 의해 왜성을 나타내는 토마토를 선발하였다. 선발된 토마토에 대해 이차원 전기영동법으로 단백질체를 분석한 결과, 발현차를 나타내는 28개의 단백질 spot이 분리되었다. 분리된 단백질 spot 중 현저하게 발현이 억제된 단백질 spot 6개를 선발하여 단백질 서열을 결정하였다. 실험결과, pI 4.5, 분자량 24 kDa를 나타내는 단백질은 브라시노스테로이드 생합성에 관여하며 왜성 표현형을 나타내는 신호전달 단백질, BAK1으로 동정되었다. BCK1, cystein proteinase, sulfutase, peroxidase, zinc finger factor로 동정된 나머지 단백질들은 브레시노스테로이드 생합성관련 신호전달기작에 관여하는 단백질로 추정되었다. BAK1을 검정하기 위해 단백질 서열이 결정된 부위로부터 프라이머를 디자인하여 RT-PCR를 수행한 결과, 증폭된 500 bp의 산물이 정상과 발현차를 보여주었는데 이 결과는 양분조절에 의해서도 BAK1의 발현이 조절될 수 있음을 시사한다. Brassinolide insensitive associated receptor kinase 1(BAK1) is a critical component that play an important roles in signaling of brassinosteroid biosynthesis. Brassinosteroid-deficient and -insensitive mutants showed the characteristic of dwarf symptom. The nutrient deficient tomato showing stunt phenomenon was selected from soiless cultivation system using modified Sonneveld hydroponic solution. Twenty eight protein spots showing different expression levels compared to the control were isolated from extracts of stunted tomato leaves by 2D PAGE analyses. Significantly down-regulated 6 protein spots out of 28 protein spots were analyzed and sequenced by MALDI-TOF mass spectrometry. The protein spot having pI=4.5 and MW=24 kDa was identified as a signal protein, BAK1, which is directly related to brassinosteroid biosynthesis. In addition, five other protein spots were identified as BCK1, cystein proteinase, sulfutase, peroxidase and zinc finger factor respectively, and they were also signal proteins related to brassinosteroid biosynthesis. Furthermore, amplification of 500bp of BAK1 mRNA by RT-PCR using a primer set of peptide matched regions was inhibited conpared to that of the wild type. The results sugested that the BAK1 might be regulated at the transcription level in response to nutrition applications.
Proteomics를 이용한 고랭지 배추의 고온장해 해석
신평균(Pyung-Gyun Shin),홍성창(Sung-Chang Hong),장안철(Ancheol Chang),김상효(Sang-Hyo Kim),이기상(Ki-Sang Lee) 한국생명과학회 2007 생명과학회지 Vol.17 No.12
무더운 날씨가 지속됨으로서 고랭지배추의 생장 및 결구가 지연되고 있는 강원도 정선군 질운산(새빗재)의 600 m와 900m의 배추를 사용하여 무기성분 및 단백질 발현패턴을 분석하였다. 식물체 무기성분에서는 생장에 관련된 질소 및 인산의 부족현상과 결구에 관련된 칼슘이 부족하였다. 단백체 분석은 2차원 전기영동에 의해 전체 126개의 단백질이 분리되었고 그 중 48개의 단백질이 고도에 따라 변화하는 양상을 보여주었다. 이 중에서 30개의 단백질 서열이 결정되었는데, 해발 900 m에서 단백질 발현이 증가한 14개 중에서 oxygen- evolving proteins, rubisco activase and ATPase 등이, 해발 600 m에서는 glutathione S-transferase (1, 28 kD cold induced- and 24 kD auxin-binding proteins) and salt-stress induced protein 등 16개의 단백질 발현이 증가하였다. 이러한 단백질은 식물체 손상에 대한 보호기작을 가진 스트레스관련 단백질로 가뭄, 온도상승, 밤낮의 온도차 등의 반복으로 복합적이며 동시 다발적으로 나타나는 고온장해 현상으로 사료된다. High temperature stresses have caused growth inhibition and delayed heading in highland cultivation Chinese cabbage during summer in Korea. We have studied high temperature stress responses in the terms of changes of inorganic components and proteins by proteomic analyses. Insufficiencies of nitrogen and phosphorus have affected growth rate and calcium deficiency has caused blunted heading. Proteins extracted from Brassica seedling grown at the altitude of 600m and 900m in the Mount Jilun were extracted and analysed by 2-dimentional polyacrylamide gel electrophoresis. Profiles of protein expression was then analyzed by 2-dimentional gel analyses. Protein spots showing different expression level were picked using the spot handling workstation and subjected to MALDI-TOF MS. Total 48 protein spots were analyzed by MALDI-TOF MS and 30 proteins spots out of 48 were identified by peptide mass fingerprinting analyses. Fourteen proteins were up-regulated in extracts from the altitude of 900m and they were identified as oxygen-evolving proteins, rubisco activase and ATPase etc. Sixteen proteins were up-regulated in extracts from the altitude of 600m and they were identified as glutathione S-transferase(1, 28 kD cold induced- and 24 kD auxin-binding proteins) and salt-stress induced protein etc. These stress-induced proteins were related to the mediated protective mechanism against oxidative damage during various stresses. The results indicated that physiological phenomenon in response to high temperature stresses might be resulted by complex and multiple array of responses with drought, heat, oxidative, salt, and cold by high temperature.
AFLP 분석 및 체세포 불화합성에 의한 느타리 유사품종의 확인
서경인 ( Kyoung In Seo ),유영복 ( Young Bok Yoo ),장갑열 ( Kab Yeul Jang ),신평균 ( Pyung Gyun Shin ),오연이 ( Youn Lee Oh ),김광호 ( Kwang Ho Kim ),공원식 ( Won Sik Kong ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.1
We already reported four groups which contains some similar strains based on URP-PCR in the previous paper. The objective of this study was to confirm those strains by the amplified fragment length polymorphism (AFLP) and vegetative compatibility group (VCG). AFLP analysis showed no difference among these strains except ASI 2595 and 2183 in Weonhyeong group and ASI 2829 in Suhan group. They showed specific DNA bands only in the result of P + AG/M + AAG and P + GT/ M + ATG primer combinations out of eight different combinations. The AFLP primers produced a total of 330 fragments between 80 and 1000 bp in length for 31 Pleurotus ostreatus strains. At a genetic similarity of 0.96, the UPGMA analysis separated the isolates into four distinct clusters. Each group was classified by similar strains. Confrontation test by vegetative compatibility groups (VCGs) also showed distinct line between strains from different groups, but no line between similar strains within the cluster. Our results indicate that most of similar strains was not distinctness. Thus, similar strains are considered to be very close on the genealogy of their parent or same strain with different name.
In Vitro Translation of Nodule Specific mRNA from Alfalfa (Medicago sativa L. Vernal) Root Nodules
신평균,김경수,조무제,Shin, Pyung-Gyun,Kim, Kyung-Sao,Cho, Moo-Je 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.2
알팔파근류에 R. meliloti 102F51 및 3A150 mutant ($nif^-$, $nod^+$)를 접종하여 얻은 nodule과 접종하지 않은 뿌리로부터 $poly(A)^+-mRN$를 분리 정제하여 [$^3H$]-leucine 및 [$^{35}S$]-methionine으로 in vitro translation 시킨 후 이들을 leghemoglobin 및 nodule specific protein antibody를 이용하여 immunoprecipitation 시킨다음 2D-polyacrylamide gel 전기영동을 행한 결과 분자량 12,000-30,000 범위내에서 적어도 25종 이상의 근류에서 선택적으로 발현이 촉진되는 단백질들을 확인하였으며 이들은 대두의 경우와 상당한 차이가 있는 것으로 보아 두 과작물간에 이들 단백질 양상이 서로 차이가 있을 것으로 추정된다. Nodule specific proteins (nodulins) were detected by the 2D-polyacrylamide gel electrophoresis of the immunoprecipitated in vitro translation products of the $poly(A)^+-mRN$ A from alfalfa root nodules. At least 25 nodule specific polypeptides in the molecular weight range of 12,000-30,000 were identified in the Rhizobium meliloti 102F51 (wild type) infected alfalfa root nodules. The nodulins accounted for about 40% of [$^3H$]-leucine labeled and 12% of [$^{35}S$]-methionine labeled proteins.
팽이버섯(Flammulina velutipes)의 Genome-wide SNP (Single Nucleotide Polymorphism)에 의한 계통 분석
우성이 ( Sung I Woo ),김은선 ( Eun Seon Kim ),한재구 ( Jae Gu Han ),장갑열 ( Kab Yeul Jang ),신평균 ( Pyung Gyun Shin ),오연이 ( Youn Lee Oh ),오민지 ( Min Ji Oh ),조성환 ( Sung Hwan Jo ),이정희 ( Jeong Hee Lee ),김경수 ( Kyung So 한국균학회 2015 韓國菌學會誌 Vol.43 No.4
Genome-wide reanalyzed data of 25 Flammulina strains were compared against the reference genome (KACC42780) to establish a genome-wide single nucleotide polymorphism (SNP). The rate of mapping differences between the strains reflected in the strain variation in its result. Genome-wide SNPs distribution divided into types of homozygous SNP and heterozygous SNP moreover all of the strains demonstrated a wide variation in all of the regions. In the further study of topological relationship between the collected strains, phylogenetic tree was separated into 3 major groups. Group I contained F. velutipes var. related strains of ASI 4062, 4148, 4195. Group 2 contained strains that are different species of ASI 4188 F. elastica, ASI 4190 F. fennae, and ASI 4194 F. rossica. The other 19 strains F. velutipes were classified as a single group. However, further experiment to discriminate its genetic relationship between the white group and brown group did not verify its validity. The inferred tree exhibited a phylogenetic relationship between Korea white fruitbody forming strains of ASI 4210, 4166, 4178 and Japan white fruitbody forming strains of ASI 4209, 4167 confirmed to be genetically closely related.