The development of nano-polyplex efficacious against osteoarthropathy

손재민,박영호,이창섭 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4

This study is a manufacturing design to reduce side effects and to increase use rate of organism by inclusion of Alendronate, a kind of bisphosphonate used for treatment and prevention of osteoporosis, into the center of W1/O/W2 multi-emulsions. We have used enteric coating materials insolvent in acid by adding adhesion property of mucous membrane. We have secured enteric nature with Eudragit by using PEO (polyethylene oxide) polymer as adhesion property of mucous membrane and have encapsulated using PVA (polyvinyl alcohol). We have conducted optimum test per phases and it was found out that the best result was achieved when we have used an ideal quantity ratio of Alendronate and PEO in 1:0.1. The quantity of PEO molecule was 300,000 and oily phase was manufactured with the addition of water phase, and L100 Type Eudragit was used for the manufacturing. We have manufactured the optimum multiple-emulsions by adding 1% PVA to this. Multi-emulsions manufactured in this study had a 70% efficiency of encapsulation rate and grain ratio was 800 nm and have shown enteric stability and efficiency.

중앙은행의 디지털 화폐 발행과 시중은행의 수익성

손재민,류두진 한국경영학회 2022 經營學硏究 Vol.51 No.3

This study analyzes the impact of changes in central bank digital currency (CBDC) issuance and interest rates on commercial bank profitability, considering the interaction of individuals (savers and borrowers), commercial banks, and the government (central bank). We show that the profitability of commercial banks in the deposit sector decreases when interest rates higher than deposit rates are imposed on CBDC or liquidity constraint policies are enforced. On the other hand, if the interest rate on CBDC is lower than the deposit rate, the effect on the profitability of commercial banks is minimal, and the profitability of the loan sector is not affected by the CBDC issuance. We also suggest implications for the method of operating CBDC. If the CBDC is not operated directly by a central bank, but indirectly by commercial banks, the government's costs may decrease and the profits of the commercial banks may increase due to the business connectivity and effects of the economy of scale.

비만 성인 여성의 토르소 신체 치수 및 체형 분석

손재민,김동은,Sohn, Jae-Min,Kim, Dong-Eun 복식문화학회 2017 服飾文化硏究 Vol.25 No.5

The purpose of this study was to classify the upper torso body types of obese Korean adult women using the 7th Korea National Anthropometric Study data and compare the body type differences according to three age groups: 20s~30s, 40s~50s, and 60s. A total of 548 adult women whose BMI was in the obese range of 25 or higher and whose age ranged from 20s to 60s were selected from the anthropometric database. Twenty-nine body measurements related to torso and arm areas important for torso and sleeve pattern development were chosen. Five drop values by differences between bust, waist, and hip circumferences were also chosen for analysis. The number of obese women increased with age. The results revealed seven factors according to the factor analysis and three obese body types based on the cluster analysis. Body type 1 (47.3% of obese women) was characterized by narrow shoulders, a small or medium torso, and a straight waist. Body type 2 (42.4% of obese women) was characterized by a defined waist and a larger lower torso than upper torso. Body type 3 (10.3% of obese women), the largest obese body type among the three types, was characterized by a large bust, large abdomen area, and long upper torso. Women in their 20s to 30s were most likely to have body type 2, women in their 40s to 50s were evenly distributed between body types 2 and 3, and women in their 60s were most likely to have body type 1.

가로돌기 단독 골절로 진단받은 요통 환자에 대한 한의학적 치료 효과 : 증례 보고

손재민(Jaemin Son),박병학(Byunghak Park),이남우(Namwoo Lee),한정훈(Jeonghun Han),강도현(Dohyeon Kang),민태운(Taewoon Min),안재서(Jaeseo Ahn),이한솔(Hansol Lee),이현준(Hyunjun Lee),지형욱(Hyungwook Ji),조소현(Sohyun Cho),이성민(Seongmin 대한스포츠한의학회 2021 대한스포츠한의학회지 Vol.21 No.1

Objectives Isolated Transverse Process Fracture(ITPF) is a rare traumatic spinal disease that has been fractured in transverse process alone in vertebra. This study reports the effectiveness of Korean medicine treatment in ITPF patient. Methods The patient was treated with acupuncture, pharmacopuncture and herbal medicine for 28 days. EuroQol-5 dimension index(EQ-5D), Numerical rating scale(NRS), Oswestry disability index(ODI), lumbar Range of motion(ROM) were used for assessment. Results After treatment, NRS for low back pain decreased from 5 to 2. EQ-5D improved from 0.215 to 0.76, ODI from 82 to 31.11 and lumbar ROM also improved. Conclusions This case shows that Korean medicine treatment can be considered as effective treatment for ITPF.

갯메꽃 추출물과 유기용매 분획물의 암전이 억제 효과

손재민(Jaemin Son),김준세(Junse Kim),김호준(Hojun Kim),서영완(Youngwan Seo) 한국생명과학회 2019 생명과학회지 Vol.29 No.3

갯메꽃은 해안 사구에 분포하며 식물로 높은 환경 적응력을 가지고 있으며 갯메꽃은 항산화, 해열, 살균, 이뇨작용 등에 효과가 있는 것으로 알려져 있다. 본 연구에서는 암전이 과정에서 중요한 역할을 한다고 알려진 matrix metalloproteinases (MMPs)의 일종인 MMP-2와 MMP-9의 활성에 대한 갯메꽃의 억제효과를 methylene chloride (MC) 및 methanol (MeOH) 추출물과 조추출물, 유기용매 분획물을 시료로 하여 인체 섬유육종 HT-1080 세포를 이용하여 ELISA (Enzyme-linked immunosorbent), gelatin zymography, Wound healing assay, RT-PCR, western blot 실험을 통해 조사하였다. 갯메꽃의 MC 및 MeOH 추출물, 조추출물과 4가지 유기용매 분획물이 HT-1080 세포에서 MMP-2와 MMP-9 생성 억제 활성을 나타냄을 확인하였다. 그 중에서도 n-hexane, 85% aq.MeOH 분획물이 전반적으로 MMP-2와 MMP-9에 대한 높은 억제활성을 보였다. 이들 결과로부터 85% aq.MeOH과 n-hexane 분획물에 MMP 억제 활성이 높은 물질이 존재할 것으로 추측되어지며, 갯메꽃 추출물 및 분획물이 암침윤 및 전이를 억제하는 효과적인 항암소재로서의 가능성이 있음을 제시한다. 이에 본 연구팀은 85% aq.MeOH과 n-hexane 분획물에 있는 활성물질을 분리하기 위한 연구를 추가적으로 수행할 계획이다. Calystegia soldanella is distributed in coastal sand dunes and has high environmental adaptability; it is also known to be effective for anti-oxidant, anti-pyretic, anti-septic, and diuretic action. This study investigated the effect of crude extracts and organic solvent fractions of C. soldanella on MMP-2 and MMP-9 expression, MMP activity, and cell mobility in phorbol-12-myristate-13-acetate (PMA)-induced fibrosarcoma HT-1080 cells. C. soldanella was twice extracted, once with methylene chloride (MC) and once with methanol (MeOH). After the MC and MeOH extracts were combined, their suppressive effects on MMP-2 and MMP-9 expression, MMP enzymatic activity, and gene and protein expression were measured by gelatin zymography, enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction, and western blot method. Cell mobility for the HT-1080 cells was observed by wound healing assay. The combined crude extracts showed a significant suppressive effects on MMP-2 and MMP-9 expression. To explore active inhibitory elements, the combined extracts were fractionated according to polarity into with n-hexane, 85% aqueous methanol, n-butanol, and water. Across these four solvent fractions, MMP-2 and MMP-9 activity and cell mobility in the HT-1080 cells were all strongly inhibited by the n-hexane fraction. These results suggest that C. soldanella extract and organic solvent fractions could be used as potent MMP inhibitors for effective anti-cancer treatments to suppress cancer invasion and metastasis.

나피온 117 전해질 막의 확산 계수 계산 방법에 따른 평가

손재민(Jae Min Son),유상석(Sang Seok Yu) 대한기계학회 2017 대한기계학회 춘추학술대회 Vol.2017 No.11

무선 센서 네트워크에서의 제한된 쿼리 전달 기법

손재민(Jaemin Son),진정우(Jungwoo Jin),한기준(Kijun Han) 전력전자학회 2005 전력전자학술대회 논문집 Vol.- No.-

쿼리에 응답하는 데이터 전달방식에서는 일반적으로 쿼리가 네트워크 전체에 뿌려진다. 하지만 쿼리가 특정지역의 정보만을 요구하는 경우 그 쿼리는 네트워크 전체에 뿌려질 필요가 없다. 본 논문에서 우리는 제한된 쿼리 전달 기법을 제안한다. 제안된 기법은 네트워크를 섹터와 레벨단위의 논리적 영역을 만들고 쿼리가 필요로 하는 정보가 속한 최소한의 영역으로만 쿼리를 전달함으로써 불필요한 쿼리 전달을 줄이고자 하는 것이다. 성능평가에서는 제안된 기법을 사용함으로써 센서 노드들의 평균 소비되는 에너지를 줄이고 네트워크의 생존시간을 늘리는 결과를 보였다.

무선 센서 네트워크를 위한 효율적인 쿼리 전달 방법

손재민 ( Jae-min Son ),진정우 ( Jung-woo Jin ),한기준 ( Ki-jun Han ) 한국정보처리학회 2005 한국정보처리학회 학술대회논문집 Vol.12 No.1

센서 네트워크에서는 응용에 따라 데이터 전송 방식을 몇 가지로 나눌 수 있다. 일반적으로 쿼리 기반의 데이터 전송방식에서는 싱크가 쿼리를 네트워크 전체에 플러딩하고 그 쿼리를 수신한 노드가 그 쿼리에 해당하면 감지한 데이터를 싱크까지 보고하는 방식을 취하고 있다. 하지만 특정 지역의 정보를 요구하는 쿼리일 경우에는 그 쿼리가 네트워크 전체에 플러딩된다면 불필요한 에너지 소모가 많이 발생하게 된다. 이를 해결하기 위하여 우리는 쿼리를 네트워크 전체에 플러딩하지 않고 특정지역으로만 쿼리를 전달하는 방법을 제안한다. 또한 실험을 통해서 쿼리를 플러딩하는 방법과 특정지역으로만 전달하는 방법을 비교하여 남아 있는 노드수와 평균 소비되는 에너지를 측정하여 제안한 방법이 센서 네트워크에서 중요시되는 생존시간 측면에서 더 우수함을 보였다.

HT-1080 세포에서 만형자 용매 추출물의 암전이 억제효과

손재민(Jaemin Son),김호준(Hojun Kim),공창숙(Chang-Suk Kong),서영완(Youngwan Seo) 한국생명과학회 2018 생명과학회지 Vol.28 No.3

본 연구는 PMA (Phorbol 12-myristate 13-acetate)에 의해 MMP 활성이 증가된 섬유육종세포에서 MMP-2와 -9의 mRNA 발현 및 단백질 활성에 대한 만형자의 억제 효과를 zymography와 RT-PCR 방법에 의해서 측정하였다. 만형자 시료는 dichloromethane에 의해서 두 번 추출되었으며 동일한 과정을 methanol를 사용하여 반복하였다. 각각의 용매에 의해서 얻어진 추출물을 합한 후에 zymography를 이용하여 이 추출물의 MMP-2와 -9에 대한 억제효과를 측정한 결과 유의적인 억제효과를 나타내었다. 억제활성 성분을 추적하기 위하여 극성에 따른 추출물의 용매분획을 실시하여 n-hexane, 85% aq. MeOH, n-butanol, 및 water 분획층을 얻었다. 이 4가지 용매분획에 대한 MMP 억제활성을 측정하였으며 측정한 결과 85% aq. MeOH 분획층이 zymography와 RT-PCR 실험에서 MMP-2와 -9에 대해 가장 강한 억제효과를 나타내었다. 이상의 결과는 만형자 추출물이 암전이 억제제 개발을 위한 좋은 원천이 될 수 있는 가능성이 있음을 제시한다. Viticis fructus (fruits of Vitex rotundifolia) is the dried fruit from Vitex rotundifolia; is a traditional medicine for treating inflammation, migraines, chronic bronchitis, headaches, eye pain, and gastrointestinal infections; and demonstrates various bioactivities, including anti-allergic, anti-cancer, and anti-inflammatory effects, which are partly due to its phenolic compound content. This study examines the inhibitory effects of viticis fructus (fruits of Vitex rotundifolia) on MMP-2 and MMP-9 expression using gelatin zymography and RT-PCR in phorbol-12-myristate-13-acetate (PMA)-induced HT-1080 fibrosarcoma cells. Fruits of Vitex rotundifolia were extracted twice using dichloromethane (CH₂Cl₂) and methanol (MeOH). The combined crude extracts (CH₂Cl₂ and MeOH) significantly inhibited MMP-2 and MMP-9 activities in gelatin zymography. The combined extracts were fractionated into n-hexane, 85% aqueous methanol (85% aq. MeOH), n-butanol, and water, successively according to polarity. Among all solvent-partitioned fractions, 85% aq. MeOH fractions showed the strongest inhibition on the activation of MMP-2 and MMP-9 in gelatin zymography. In PMA-stimulated HT-1080 cells, the expression levels of MMP-2 and MMP-9 mRNA were also greatly inhibited by the 85% aq. MeOH fraction. These results suggest that viticis fructus can be used as an excellent source for anti-invasive agents.

나피온 117 전해막 확산 계수 및 수분 투과량 측정

손재민(Jaemin Son),정진희(Jinhee Jeong),유상석(Sangseok Yu) 대한기계학회 2019 大韓機械學會論文集B Vol.43 No.9

연료 전지 전해질 막의 수분 확산성은 막의 이온전도도를 결정하는 가장 중요한 요소이다. 연료전지의 운전 조건에 따라 수분 투과 구동력인 전기삼투 및 비선형 영역의 역확산으로 인한 막의 수분투과에 대해 정확한 이해와 계산 방법이 필요하다. 온도 및 습도에서의 전해질 막의 수분 확산성은 확산 계수에 의해 결정될 수 있다. 본 연구에서는 수분 확산을 결정하기 위한 3가지 접근 방법을 평가한다. 수분 확산 계수의 첫 번째 접근법은 수분함유도를 이용한 계산이며, 두 번째 접근법은 테스트 지그양단 평균 농도 계산 방식에 따른 NMR 방법과 비교, 세 번째 접근법은 막 표면 물질전달계수의 저항을 이용하는 것이다. 실험은 기체의 3가지 온도(30˚C, 50˚C, 70˚C)와 습도(0%~100%)에 대해 수행했으며, 지그의 입구 습공기 농도를 기준으로 계산을 진행했을 때 기존 NMR 결과와 잘 맞았다. 대류물질전달 계수의 영향은 10-12~10-14c㎡/s 정도로 매우 미미한 것을 알 수 있다. The water diffusivity of the fuel cell electrolyte membrane is the most important factor that determines the ionic conductivity of the membrane. Owing to the existence of electro-osmosis, which is a water-permeable driving force, and reverse diffusion, which is a nonlinear region, depending on the operating conditions of the fuel cell, an accurate understanding and calculation method for water permeation of the membrane is required. The water diffusivity of the electrolyte membrane is determined by the water diffusion coefficient in terms of temperature and humidity. In this study, three different approaches are evaluated to determine water diffusion. In the first approach, water diffusivity is calculated with measured water content. In the second approach, experimental data is compared with the NMR method. In the third approach, the membrane surface resistance and mass transfer coefficient are used. Experiments are carried out at three different temperatures (30˚C, 50˚C, and 70˚C) and humidities (0~100) of gases. The test jig is well fitted with the existing NMR results when the calculation is based on the inlet water concentration. The effect of convective mass transfer coefficient is also small enough to be negligible.