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서조은,전상옥,오동호,이상길 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Compound K (CK)-loaded polymeric micelle (CK-PM) was prepared using Pluronic F-127. The mean particle size of CK-PM was 254 ± 23.45 nm. FT-IR and DSC analysis has shown physical interaction between CK and Pluronic F-127. In PXRD, the prominent crystalline peak of CK-PM situated at 19.19° and 23.4° (2θ) was clearly observed, however the disappearance of intense peak of CK was confirmed by polymeric micelle formulation. In Caco-2 cell permeability study, (Papp of PM/Papp of CK) was enhanced about 1.2-fold. In addition the oral bioavailability of CK was enhanced 2.6-fold by PM.
Enhanced topical delivery of fish scale collagen employing negatively surface-modified nanoliposome
서조은,김민주,전상옥,오동호,윤규형,최영욱,Santosh Bashyal,이상길 한국약제학회 2018 Journal of Pharmaceutical Investigation Vol.48 No.3
In this study, we investigated the effect of fish scale collage (FSC) on type I procollagen expression in cultured human dermal fibroblasts using real-time polymerase chain reaction and ELISA, under normal and UV exposure condition. Also, FSC encapsulated negatively surface modified nanoliposome ( NLsurf mod) was fabricated, and the physicochemical characteristics of the liposomes and skin permeation properties were evaluated. FSC increased type I procollagen mRNA and protein expression in human dermal fibroblasts. The mean particle size, zeta potential value and encapsulation efficiency of the NLsurf mod were about 175 nm, −44 mV and 11%, respectively. The skin localization effect was enhanced by NLsurf mod. Conclusively, FSC is able to elevate the type I procollagen expression in human dermal fibroblasts and FSC-loaded NLsurf mod would be a good candidate for the topical delivery of FSC.
Anti-aging effects of fish scale collagen peptide
서조은,전상옥,이상길 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
UV irradiation causes a photoaging, results in decrease of type I procollagen and increase of matrix metalloproteinase-1(MMP-1) in human skin and cultured dermal fibroblast. In this study, we investigated the effect of fish scale collagen peptide on type I prcollagen in cultured human dermal fibroblasts. After treatment of fish scale collagen peptide (Mw: c.a. 700 Da), the mRNA and the protein levels of type I procollagen was measured by real-time polymerase chain reaction and ELISA, respectively, in normal and UV exposure condition. We found that fish scale collagen peptide increased type I procollagen protein expression dose-dependently in human dermal fibroblasts. Our results suggest that fish scale collagen peptide plays an important role on recovering the type I procollagen expression in human dermal fibroblasts.
오동호,전상옥,서조은,이상길 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
sCT-STPP ionic complex was fabricated based on our previous protocols (J controlled Rel 143 (2010) 251-257) and characterized with TEM, DSC and FT-IR. In vitro release test, cAMP release study using T47D breast cancer cell line, LLC-PK1 cell permeation study, in vivo pharmacokinetic and pharmacodynamic studies were performed. The particle size was around 50-100 nm and the complexation efficiency was c.a. 98~99%. sCT was released for 2-3 weeks in vitro. sCT permeation of 1:50 formulation through LLC-PK1 cell was superior than other formulations. The in vivo study has shown that sCT/STPP ionic complexes exhibited superior hypocalcemic effects compared to free sCT.
황희진,Sunhui Han,전상옥,서조은,Dong-Ho Oh,Seong-Wan Cho,최영욱,이상길 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.8
For the present study, rhEGF was encapsulated into solid lipid nanoparticles (SLNs). The SLNs were prepared by the W1/O/W2 double emulsification method combined with the high pressure homogenization method and the physical properties such as particle size, zeta-potential and encapsulation efficiency were measured. The overall particle morphology of SLNs was investigated using a transmission electron microscopy (TEM). The percutaneous skin permeation and accumulation property of rhEGF was evaluated using Franz diffusion cell system along with confocal laser scanning microscopy (CLSM). The mean particle size of rhEGF-loaded SLNs was 104.00 ± 3.99 nm and the zeta-potential value was in the range of −36.99 ± 0.54 mV, providing a good colloidal stability. The TEM image revealed a spherical shape of SLNs about 100 nm and the encapsulation efficiency was 18.47 ± 0.22%. The skin accumulation of rhEGF was enhanced by SLNs. CLSM image analysis provided that the rhEGF rat skin accumulation is facilitated by an entry of SLNs through the pores of skin.
전상옥,( Santosh Bashyal ),서조은,오동호,이상길 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Recombinant human epidermal growth factor (rhEGF) was encapsulated into nano-liposomes (NLs) system for topical delivery. The rhEGF-loaded NLs were prepared using a high pressure homogenization method. Morphology and overall particle distribution of NLs were investigated using transmission electron microscopy (TEM) and high resolution microscope (CytoVivaTM). Particle size, zeta (z) potential and encapsulation efficiency were measured and the percutaneous delivery of NLs was evaluated using Franz diffusion cells and immunofluorescence confocal laser scanning microscopy (CLSM). The mean particle size, z potential and encapsulation efficiency of the NLs were 155.57±2.59 nm, -57.92±4.35 mV and 9.00±0.39%, respectively. TEM and microscopic analysis showed spherical, very even-sized vesicles approximately 150 nm. The skin permeation and localization of rhEGF were enhanced by NLs. CLSM image analysis provided that the NLs enhanced the permeation and localization of rhEGF in rat skin by facilitating entry through pores of skin.
Sun-Mi Hong,전상옥,서조은,Kyeung-Hwa Chun,Dong-Ho Oh,최영욱,이도익,정성훈,강재선,이상길 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.11
Compound K (CK) was formulated as polymeric micelles (PM) using Pluronic® F-127 to enhance the oral absorption of CK, an intestinal bacterial metabolite of ginseng protopanaxadiol saponin. The physicochemical properties of Ck-loaded PM were characterized and an in vitro transport study using the Caco-2 cell system as well as an in vivo pharmacokinetic study using SD rats was carried out. The hydrodynamic mean particle size of CK-loaded PM (CK-PM) was 254 ± 23.45 nm after rehydration and the drug loading efficiency was ca. 99.9%. The FT-IR spectroscopy, X-ray diffraction, differential scanning calorimetry and scanning electron microscopy data supported the presence of a new solid phase in the PM. The Papp value of in vitro Caco-2 cell permeation of CK- PM and the oral absorption of CK was enhanced about 1.2-fold and 2.6-fold compared to CK suspension, respectively, showing that the present PM formulation enabled an enhancement of oral CK absorption.
Vaccine adjuvants: smart components to boost the immune system
Rakesh Bastola,Gyubin Noh,금태광,Santosh Bashyal,서조은,최재웅,오연수,조영식,이상길 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.11
Vaccination is an effective approach to preventthe consequences of infectious diseases. Vaccinesstrengthen immunity and make individuals resistant toinfections with pathogens. Although conventional vaccinesare highly immunogenic, they are associated with somesafety issues. Subunit vaccines are safe, but they requireadjuvants to stimulate the immune system because of theirweaker immunogenicity. Adjuvants are entities incorporatedinto vaccines to increase the immunogenic responsesof antigens. They play a crucial role in increasing thepotency and efficacy of vaccines. Different adjuvants havedifferent modes of action; therefore, a better understandingof their immunology could provide guidance for thedevelopment of novel adjuvants. Numerous studies havebeen conducted using different types of adjuvants tocharacterize their potency and safety; however, in practice,only few are used in human or animal vaccines. Thisreview aims to introduce the different modes of action ofadjuvants and give insight into the types of adjuvants thatpossess the greatest potential for adjuvanticity.