http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Detection and Characterization of Extracellular Phospholipase $A_2$ Activity in Human Amniotic Fluid
백석환,이승호,장현욱,Baek, Suk-Hwan,Lee, Seung-Ho,Chang, Hyeun-Wook 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3
사람의 양수 중에서 처음으로 세포외성 phospholipase $A_2$ 활성이 있음을 확언하였으며, 출산에 있어서 중요한 prostaglandin의 전구체인 arachidonic acid 제공에 중요한 역활을 할 것이라고 생각된다. 본 효소는 지금까지 보고된 group I이나 group II phospholipase $A_2$와 계변 활성제의 영향이나 monoclonal antibody와의 반응성에서 다른 특성을 갖는 효소로 추정되었으며, 또한 적어도 2종류의 phospholipase $A_2$의 활성이 존재함에 시사되어 금후 더욱 구체적인 검토를 행하여 단백화학적 특성을 규명할 필요가 있다. Extracellular phospholipase $A_2$ activity has been identified in human amniotic fluid. This enzyme required $Ca^{2+}$ ion and exhibited bimodal pH optimum at pH 7.0 and 9.0. The phospholipase hydrolyzed 1-acyl-2-[1-$^{14}C$]arachidonoylphosphatidylethanolamine to form only [$^{14}C$]arachidonic acid indicating that the enzyme had phospholipase "$A_2$" activity. Ionic and non-ionic detergents caused loss of enzymatic activity. This phospholipase $A_2$ was recognized, in part, by a monoclonal antibody raised against phospholipase $A_2$ from human synovial fluid. This finding suggests that our enzyme is an another type of an extracellular phospholipase $A_2$ which may not belong to the 14 KDa group II phospholipase $A_2$ family.
사람 양수 중의 세포외성 Phospholipase A2 특성
백석환,이승호,장현욱 ( Suk Hwan Baek,Seung Ho Lee,Hyeun Wook Chang ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3
Extracellular phospholipase A₂ activity has been identified in human amniotic fluid. This enzyme required Ca^(2+) ion and exhibited bimodal pH optimum at pH 7.0 and 9.0. The phospholipase hydrolyzed 1-acyl-2-[1-^(14)C]arachidonoylphosphatidylethanolamine to form only [^(14)C]arachidonic acid indicating that the enzyme had phospholipase $quot;A₂$quot; activity. Ionic and non-ionic detergents caused loss of enzymatic activity. This phospholipase A₂ was recognized, in part, by a monoclonal antibody raised against phospholipase A₂ from human synovial fluid. This finding suggests that our enzyme is an another type of an extracellular phospholipase A₂ which may not belong to the 14 KDa group II phospholipase A₂ family. v
정규찬,백석환,남경수,Chung, Kyu-Charn,Baek, Suk-Hwan,Nam, Kyung-Soo 대한약학회 1988 약학회지 Vol.32 No.1
Effects of acanthopanax cortex extracts on glutathion S-transferase (GST), glutathion peroxidase (GSH-px) and superoxide dismutase (SOD) activities related to 7,12-dimethyl-benz(a)anthracene(DMBA) metabolism and on DMBA-induced mutagenicity were investigated in this study. From the comparative study of three extracts, it was found that butanol extract was more potent than other extracts in increment of GST, GSH-px and SOD activities and in inhibitory effects of lipoperoxide formation of liver. Also ether and butanol extracts inhibited DMBA-induced mutagenicity, showing 33% to 36% of inhibition at maximum, when ether and butanol extracts were administered to rats intraperitoneally.
연구논문 : 생명과학 ; 금마타리에서 분리한 나르도스타틴의 NO, PGE2 및 TNF 생성 억제효과
주혜경 ( Hye Kyung Ju ),백석환 ( Suk Hwan Baek ),안인파 ( Ren Bo An ),배기환 ( Ki Hwan Bae ),손건호 ( Kun Ho Son ),김현표 ( Hyun Pyo Kim ),강삼식 ( Sam Sik Kang ),이승호 ( Sung Ho Lee ),손종근 ( Jong Keun Son ),장현욱 ( Hyeun Wook 영남대학교 약품개발연구소 2004 영남대학교 약품개발연구소 연구업적집 Vol.14 No.-
사람 양수중 다종의 세포외성 포스포리파제 A2의 부분정제 및 특성
전용주(Yong Ju Jeon),백석환(Suk Hwan Baek),이지혜(Jee Hae Lee),문태철(Tae Chul Moon),민병우(Beong Woo Min),장현욱(Hyeun Wook Chang) 대한약학회 1997 약학회지 Vol.41 No.2
Multiple forms of extracellular phospholipase A2 have been detected in human amniotic fluid (HAF). When HAF was subjected to heparin-Sepharose column chromatography, phospholipase A2 activity was detected in both heparin-non binding and binding fraction. The activity of heparin-non binding fraction was further purified by sequential uses of column chromatographies on butyl-Toy-opearl 650M and DEAE-Sephacel. DEAE-Sephacel fraction contained three different phospholipase A2 activities (Peak I, II, III). The molecular weight of DEAE-Sephacel fraction phospholipase A2 determined by SDS-PAGE were about 52KDa (Peak I). Peak II, III required micromolar Ca2+ ion for its maximum activity, but Peak I enzyme showed calcium independent phospholipase A2 activity and showed broad range of pH (6.0~10.0) optimum. All these enzymes were not recognized by a monoclonal antibody raised against phospholipase A2 from human synovial fluid. These results suggest that HAF might contain multiple forms of extracellular phospholipase A2, which may neither belong to the 14KDa group II phospholipase A2 family nor cytosolic phospholipase A2.
Toll-like receptor 9-매개에 의한 matrix metalloproteinase-9 발현에서 NFκB의 역할
이상훈(Sang-Hoon Lee),진병로(Byung-Rho Chin),백석환(Suk-Hwan Baek) 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.6
Background: CpG DNA plays an important role in immune cell function. This study examined whether the temporal control of toll-like receptor(TLR)9 by CpG DNA can regulate the expression of matrix metalloproteinase-9 (MMP-9). Methods and materials: Macrophages were cultured in the presence of 10% FBS. For the various MMP genes analysis, RT-PCR and real-time PCR were performed. In addition, zymography assay performed for the MMP activity. The phosphorylation assay did for the ERK1/2 and NFκB activation, and luciferase promoter assay was for the NFκB activity. Results: CpG DNA induced the mRNA expression of MMP-2, MMP-9, and MMP-13, but not of MMP-7, MMP-8, and MMP-12, in a time-dependent manner. Especially, the mRNA expression of MMP-9 was strongly induced by CpG DNA using real-time RT-PCR. The TLR9 inhibitor, chloroquine, suppressed CpG DNA-induced MMP-9 expression and its activity. Moreover, CpG DNA induced the phosphorylation of ERK and the inhibition of ERK by U0126 suppressed CpG DNA-induced MMP-9 expression and its activity. CpG DNA stimulated IκB-αdegradation and luciferase activity. In addition, pretreatment of SN-50, the inhibitor of NFκB, strongly blocked the CpG DNA-induced MMP-9 expression and activity. Conclusion: These observations suggest that CpG DNA may play important roles in the activation of macrophages by regulating the production of MMP-9 via the sequential TLR9-ERK-NFκB signaling pathway.
Jurkat T 면역세포에서 Phosphoinositides 의 가수분해를 증가시키는 약용식물 추출물의 검색
민도식(Do Sik Min),이영한(Young Han Lee),백석환(Suk Hwan Baek),서판길(Pann Ghill Suh),류성호(Sung Ho Ryu) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.2
Activation of the T lymphocytes results in a variety of early biochemical events ultimately leading to cell proliferation and lymphokine production. Stimulation of the signal transduction cascade in T cells through the T cell receptor coincides with activation of the phosphatidylinositol-phospholipase C (PI-PLC) pathway. Therefore, we have established a model system to screen immuno-simulator that can increase the hydrolysis of phosphoinositides in human T cell leukemia Jurkat cells. As a result of screening from herbal medicine extract, 4 extracts (Olibanum, Ephedrae Herba, Real Gar, Saussureae Radix) were found to increase the production of inositol phosphates. All the active fraction from the four kinds of extract were eluted in a different retention time on C-18 HPLC and these active fraction also showed difference in cell specificity. And all the active fractions increased DNA synthesis in T cell. Therefore, it is suggested that the active fraction among 4 extracts might contain a compound having different properties one another.
금은화(Lonicerae Flos)의 Ethyl Acetate 분획이 돌연변이원성에 미치는 영향
정규찬(Kyu Charn Chung),권동렬(Dong Yeul Kwon),백석환(Suk Hwan Baek),김성환(Sung Hwan Kim),장현옥(Hyeun Wook Chang) 대한약학회 1988 약학회지 Vol.32 No.5
Based on the following tests with fractions extracted from organic solvents such as benzene, chloroform, ethyl acetate and methanol, this study is to analyse the antimutagenicity of Lonicerae Flos. When carrying out Ames test with Salmonella typhimurium strain, it seemed that there was stronger antimutagenicity in TA 100 treated by MNNG than did one in TA 98 by NPD, and that there was stronger antimutagenicity through base pair exchange than one through frame shift. In the umu test, each fraction tended to inhibit the activity of beta-galactosidase induced by AF-2. As shown in these above tests, the ethyl acetate fraction was the strongest among four fractions. On the other hand, its component consisted of luteolin, apigenin, chlorogenic acid and five unknown ingredients. Of these unknown ingredients, E, F strongly tended to inhibit the activity of beta-galacosidase. In addition, there was also the decrease in its activity of apigenin, luteolin and chlorogenic acid.