졸업을 하고나서

백낙주 중앙대학교 1978 無影燈 Vol.3 No.1

서울시내 각종연구수 동물실내 흰쥐의 Hantavirus감염조사

이호왕,백낙주,김효덕 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

Hantaan virus was first isolated in 1976 from field mice, Apodemus agrarius and in 1978 from the patient of Korean hemorrhagic fever in Korea by Lee and Lee. Hemmorragic fever with renal syndrome is a major public health problem throughout most of European and Asian land mass. Although predominantly associated with rural area, it is now being reco- gnized as an urban problem and as a particular hazard to laboratory staff using rodents for biomedical research. Hantavirus is a new Genus name which includes Hantaan virus, Seoul virus, Prospect Hill virus and Puumala virus in Byunyaviridae. Since 1976 there were outbreaks of Hemorrhagic fever with renal syndrome among personnel of animal rooms of medical centers in Korea and in Japan wehere Hantavirus experiments were never done, laboratory rat infection with Hantavirus in animal rooms of six different institutes in Seoul was studied. The results are as follows: 1. The Hantaan viral antigen were demonstrated in the pulmonary tissues of 41 (22.9%) rats out of 179 laboratory rats tested. 2. The immunofluorescent antibodies against Hantaan virus were demonstrated in 64.6% (126) of sera from 195 rats in the animal rooms of six different institutes. 3. The high levels of immunofluorescent antibodies reactive with Hantaan virus were detected in 26.7% (12) of sera from 45 personnel of animal rooms of six institutes. Although all of 12 persons developed symptoms of Hemorrhagic fever with renal syndrome, they were diagnosed as influenza by clinical physicians. 4. There was no difference in susceptibility of four kinds of laboratory rats to Hantaan virus. The above results indicated for the first time that several kinds of infected laboratory rats were reservoir of Hantavirus in the animal rooms of institutes in seoul.

한국주둔 미국군인과 서울시내 미국군사 시설내 집쥐의 Hantaan virus감염조사

이호왕,백낙주,주용규,구인회 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

인천 집쥐에서 한탄바이러스와 유산한 바이러스의 분리

이호왕,백낙주,주용규,강병남 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

Korean haemorrhagic fever (KHF) was recognized for the first time in Korea in 1951 during Korean War among United Nations troops in the central front although similar diseases to KHF had been reported as Haemorrhagic nephrosonephritis in Russia, as Epidemic haemorrhagic fever in China, as Nephropathia epidemica in Scandinavia and as Epidemic haemorrhagic fever in eastern Europe and in Japan. In 1976 Lee and Lee discovered the etiologic agent of KHF in the lungs of the striped field mouse, Apodemus agrarius, which gave specific immunofluorescent reaction with sera from patients convalescent from KHF for the first time in Korea. In 1978 Lee et al. Had isolated the same agent from KHF patients sera and had succeeded the propagation of the virus in Apodemus agrarius, and it was named Hantaan virus after Hantaan river. Natural reservoirs of Haemorrhagic fever with renal syndrome are not only field mice and laboratory rats but also wild urban rats. Therefore, we have collected the wild urban rats in the international port of Incheon for serologic survey of Hantaan virus infection and carried out isolation of Hantaan-like virus from infected rats. 1. Among the 215 wild urban rats collected in the international port of Incheon, 112 (52%) had antibodies against Hantaan virus and 51(24%) had Hantaan virus-like antigen. 2. Fourteen strains of Hantaan-like virus were isolated from S.D. rats inculated with the suspension of lungs from 60 wild urban rats. One strain of Hantaan-like virus was isolated from S. D. rats inoculated with the antigen negative lung tissues from 10 urban rats that contained antibodies in the sera against Hantaan virus. In positive urban rats, the largest amount of Hantaan-like virus antigen was distributed in lungs, followed by spleen, liver and kidneys, in that order. 3. 82-3 strain isolated from urban rats in Incheon was cultured in Vero E6 cells, and this virus had antigenicities of both Hantaan and Punmala viruses which cause haemorrhagic fevers in Korea and Scandinavia by immunofluorescent antibody technique. Further study to characterize this Hantaan-like virus is in progress.

한국형출혈열 병원체 Hantaan virus에 대한 뇨의 살균작용

이호왕,백낙주,양상기 대한감염학회 1981 감염 Vol.13 No.1

Korean hemorrhagic fever(KHF) reported for the first time in Korea in 1951, althought it had previously been known from both Manchuria and Russia. Since that time it has been known as Korean hemorrhagic fever and has remained endemic near the Demilitarized Zone between North and South Korea. In 1976 and 1978, Lee et al reported the isolation of Hantaan virus, the etiologic agent of KHF, from the patients and Apodemus agrarius coreae collected in the endemic areas of KHF and developed Lee's method of serological diagnosis of KHF. Very recently, Lee et al demonstrated virus excretion in the urine of infected Apodemus agrarius for a long time. The urine from both Apodemus agrarius and susceptible human were examined its effect on the infectivity of Hantaan virus to Apodemus mice. The results are summarized as follows: 1) The urine from Apodemus agrarius rodent, the natural reservoir of Hantaan virus, had higher activity to inactivate the infectivity of Hantaan virus than that of human. 2) The urine from Apodemus agrarius just after sampling was pH 8.3 and it had stronger antiviral activity than the urine adjusted to pH 7.2. 3) pH of human urine just after sampling was 6.1 and this urine decreased 10~100 10? of the virus after 3 hours in room temperature, but the urine with pH 7.2 had no change to infectivity of the virus.

신증후 출혈열의 임상진단과 혈청진단

이호왕,백낙주,주용규,오명환 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.1

Epidemic hemorrhagic fever was known to exist in Korea for the first time in 1951 among soldiers of the United Nations during Korean War. Since that time it has been known as Korean hemorrhagic fever (KHF). In 1976 Lee and Lee successfully discovered an antigen in the lungs of Apodemus agrarius coreae which gave specific immunofluorescent reaction with convalescent sera from patients of KHF. In 1978, Lee et al. Isolated this antigen from blood of KHF patients and perfected a specific serological test for diagnosis of KHF. The aetiologic agent of KHF was identified as a new member of Bunya- viridae and was named Hantaan virus after the Hantaan river which runs near the 38th parallel between South and North Korea. A close aetiological relationship was established between KHF and hemorrhagic fever with renal syndrome in USSR, nephrophathia epidemica in Scandinavia and epidemic hemorrhagic fever in Cliina and in Japan. The working group on HFRS at a WHO meeting in Tokyo in February 1982 recommended that KHF-like diseases with different names in Euro-Asia continent should be referred to as Hemorrhagic fever with renal syndrome (HFRS). This report describes the difference between clinical diagnosis and specific serological diagnosis of HFRS. The indirect IF antibody technique was performed for serologic diagnosis of the patients having a symptom of HFRS from 1982 to 1984 and the results are summarized as follows. 1. Specific serological diagnosis of the 3571 cases of suspected HFRS clinically showed that about 50% was proven to be of definite HFRS. 2. Blined samples of 205 sera-from the hospitalized patients was used for the specific serological diagnosis of HFRS, 4 cases were diagnosed to be HFRS serologically and of which, 2 cases were diagnosed as HFRS clinically but 1 case, chronic renal failure, and the other, G-I bleeding. 3. Patients diagnosed as G-I bleeding and acute abdomen in early stage of HFRS should go through detailed specific serological diagnosis of HFRS since all of eight cases of such patients were expired after surgical operation.

한국형 출혈열의 자연계 숙주 Apidemus agrarius의 번식기에 관한 연구

이호왕,백낙주,두창대 대한바이러스학회 1981 Journal of Bacteriology and Virology Vol.11 No.1

The natural reservoir host of Korean hemorrhagic fever is Apodemus agrarius coreae in the rural endemic areas in Korea. Hantaan virus, the etiologic agent of Korean hemorrhagic fever, is excreted from the urine, fecal material, and salivary fluid of the infected Apodemus agrarius. The serologic diagnostic method was accomplished by the use of infected lung tissue of Apodemus mice and A 549 cells with Hantaan virus as antigen by indirect immunofluorescent antibody technique. Authors surveyed and analized the seasonal distribution and the number of pregnant Apodemus agrarius which have heen collected at Jejudo, Chindo, and Songnaeri during the period from January 1975 to December 1980 for 5 years. This paper identified breeding season of Apodemus agrarius in Korea for the first time. The results were as follows: 1. The pregnant Apodemus agrarius was presented 2 peaks in an year and large number was collected at April-June, and August-October which was demonstrated to have close relationship with the appearance of Korean bemorrhagic fever patient. 2. The number of the collected Apodemus egrarius by sex was as following; male was abundant in November, December, January, February and March but female was abundant in April, May, June, August, September and October. 3. The collected number of Apodemus agrarius by the year for 5 years was not different and almost same numbers were collected in every year.

한국형 출혈열의 병원체 Hantaan virus에 대한 virazole의 작용

이호왕,성인화,백낙주,이정인 대한바이러스학회 1981 Journal of Bacteriology and Virology Vol.11 No.1

The effects of virazole to Hantaan virus, the causative agent of Korean hemorrhagic fever (KHF), were observed in the experiments in vivo of Apodemus agrarius coreae, the reservoir of KHF in the rural endemic areas of Korea. The following results were obtained. 1. When intramuscular injection of virazole was done, up to 50,ug/kg/day in amount for a few days, relative toxic effects were observed. And when 25 ug/kg/day was injected intramuscularly for several days, no specific toxic effects were observed. 2. After injection of Hantaan virus into Apodemus agrarius coreae, the virus proliferation was suppressed by consecutive injection of virazole right after inoculation of the virus for 5 days. 3. Since Apodemus agrarius coreae had no clinical symptoms despite of Hantaan viral multiplication, the effects of virazole to the course of illness and symptoms were not able to detect. Above results showed that some preventive pharmacological effects of virazole on Hantaan virus infection in vertebrate were suspected by demonstration of inhibition of virus multiplication after injection of virazole into infected Apodemus agrarius coreae on appropriate timing.

한국형 출혈열 : Ⅵ. Some epidemiologic study in the endemic areas Ⅵ 유행지역의 역사적 조사

이호왕,이평우,백낙주,황영희 대한감염학회 1981 감염 Vol.13 No.1

Korean haemorrhagic fever(KHF) occurred for the first time in korea during Korean war among UN forces residing in Yunchun and Chulmon areas in 1951, although similar disease had previously been known to both the Japanese and Russian in China and in Siberia, respectively. After Korean War, the disease has been fixed in the areas of Demilitarized Zone(DMZ) as an endemic one, and the causative agent has gradually spread in a southwestrly direction and from 300 to 800 hospitalized cases have been reported every year. Very recently the etiologic agent of KHF was isolated by Lee et al, and found to be a virus carried by the rodent Apodemus agrarius coreae. Diagnostic increase in specific immunofluorescent antibodies to Hantaan virus was demonstrated in patients suspected of having KHF. By the same technique KHF-related illness has also been diagnosed in Russia, in Finland, in Sweden, in China, in India, in Greece and in Japan. The epidemiology of both seasonal incedence of KHF and infected Apodimus agrarius with Hantaan virus in Korea was not understand well till now. Summary of the present study carried out from 1974 to 1978 is as follows. 1. There were 10 species of wild rodent in the field of hyperendemic areas of KHF. The most prevalent species was Apodemus agrarius coreae 76%, Microtis fortis pelliceus 9.8% and Crosidura lasiura 7.4% were next in order. 2. It was shown that there were always 2 peaks of KHF patient in an year in the areas of DMZ from 1974 to 1977, small peak in the late spring and large peak in the late fall. 3. Seasonal distribution of infected Apodemus agrarius coreae with Hantaan virus in the endemic areas from 1975 to 1977 showed two peaks, one in May and June, and the other in September, October and November. 4. It was found that peaks of number of Apodemus agrarius coreae with Hantaan virus were proceeded always about one month prior to the peaks of KHF patients in the hyperendemic areas of KHF. 5. Percent of infected Apodemus agrarius coreae with Hantaan virus among total Apodemus population in the endemic areas of KHF was 11~17%. The above results indicate clearly close epidemiologic relation between KHF patient and infected Apodimus agrarius coreae with Hantaan virus.

각종 동물의 한탄바이러스 및 서울바이러스에 대한 감수성관 각국 신증후 출혈열 환자 혈청의 이들 바이러스에 대한 항체가의 비교

이호왕,김재기,주용규,백낙주 대한바이러스학회 1988 Journal of Bacteriology and Virology Vol.18 No.1