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박진병 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
C9 to C13 ω-hydroxycarboxylic acids, ω-aminocarboxylic acids, and α,ω -dicarboxylic acids, which are widely used for the production of various chemical products and intermediates, can be produced from renewable oils and fatty acids (e.g., soybean oil, microalgae oil, oleic acid, linoleic acid, ricinoleic acid)<sup>1-4</sup>. ω-Hydroxycarboxylic acids, which were produced from oxidative cleavage of fatty acids via enzyme enzymatic reactions involving fatty acid double bond hydratases, alcohol dehydrogenases, Baeyer-Villiger monooxygenases and esterases, were further converted into ω-aminocarboxylic acids or α,ω-dicarboxylic acids by the AlkJ from Pseudomonas putida GPo1 and ω-transaminase from Silicibacter pomeroyi <sup>1,2</sup>. The recombinant Escherichia coli expressing the catalytic enzymes and the whole-cell based biocatalytic process were constructed to produce a variety of C9 to C12 carboxylic acids including 1,9-nonanedioic acid, 1,10-dec-6-enedioic acid, 11-hydroxyundec- 9-enoic acid, 11-aminoundecanoic acid, and 12-hydroxydodec- 6,9-dienoic acid from ricinoleic acid, oleic acid, linoleic acid or γ-linolenic acid to high yield <sup>1-5</sup>. This study thus contributes to the preparation of functional carboxylic acids from renewable unsaturated fatty acids.
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3`UTR engineering to improve functional expression of insoluble enzymes in Escherichia coli
박진병 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Functional expression of the catalytic enzymes in microbial cells is essential to produce chemical products via whole-cell biocatalysis. Thereby, 3`-untranslated region (3`UTR) engineering was investigated to improve solubility of the heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of the gene fragments containing putative RNase E recognition sites into the 3`UTR of the BVMO genes led to reduction of the corresponding mRNA level in E. coli BL21(DE3). However, the amount of BVMOs in soluble fraction was remarkably enhanced resulting in a proportional increase of in vivo catalytic activity. The increase of whole-cell biocatalytic activity notably correlated to the number of putative RNase E endonucleolytic cleavage sites in the 3`UTR. For instance, 12-keto oleic acid biotransformation activity of the BmoF1 of Pseudomonas fluorescens DSM50106 was linear to the number of RNase E cleavage sites in the 3`UTR.
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박진병,서홍관,김철환,장경호 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5
연구배경 : 연의 유해성에 대한 사회적 인식이 점차 확대되어가지만 아직도 청소년의 흡연율은 계속 증가하고 있다. 청소년 흡연율의 증가는 성인흡연으로의 고착화 가능성을 증가시키며, 그들의 신체와 정신을 병들게 한다. 이러한 상황 속에서 현재 청소년의 흡연율 파악은 비밀 보장이 완전하지 못한 상태에서 설문조사에만 의존하고 있는 실정이다. 그러므로 객관적인 검사방법에 의한 흡연율을 구하고자 하였으며, 청소년들의 흡연에 관련되는 특성들을 파악하여 흡연예방과 금연유도에 필요한 자료를 얻기 위해 본 연구를 시행하였다. 방법 : 1999년 5월에서 6월까지 임의로 서울시내 남녀 고등학교 1개씩을 정하여 3학년 100명씩(2학급)을 대상으로 미리 준비한 자기기입식 설문조사를 실시하고, 그 인원을 대상으로 충분한 설명을 한 후에 일산화탄소 측정기를 통해 호기내 일산화탄소의 농도(ppm)를 측정하여 설문지의 흡연율과 비교하고 흡연행태, 니코틴 의존도, 가족기능도지수 등을 각각 남녀 별로 비교 분석하였다. 결과 : 대상 학생들의 설문지에 의한 흡연율은 전체적으로 19.5%로 이중 남자는 34.0%였고, 여자는 5.8%였고, 일산화탄소 측정기에 의한 흡연율은 남녀 각각 20.6%, 2.9%였다. 매일 한 개피 이상 피우는 매일 흡연자는 흡연자의 84.6%였고, 흡연량은 남자에서는 6-20개피가 48.5%로 제일 많고, 여자에서는 5개피 이하와 6-20개피가 각각 50%로 나타났다. 흡연동기는 "그냥 호기심에서"가 남자에서 39.4%로 제일 많았고 여자에서는 "친구의 권유나 어울리기 위해서"가 50.0%로 제일 많았다. 흡연 시작 시기는 남자에서는 고교 1학년 때와 중학교 2학년 때가 25.6%로 제일 많았고 여자에서는 66.7%가 중학교 1학년 때 흡연을 시작하였다. 흡연을 주로 하는 장소로는 남자는 길거리가 제일 많았고, 여자는 카페, 커피숍이 제일 많았으며, 담배의 주 구입원은 두 군 모두 슈퍼나 가게가 많았고, 남자는 호주머니에, 여자는 가방에 주로 담배를 소지하였다. 두 군 모두 친구들과 주로 흡연을 하였으며, 흡연에 대해 남자는 아무렇지 않게 생각하며, 여자는 불안한 가운데 흡연을 하는 것으로 나타났다. 흡연자의 니코틴 의존도는 고도의 의존도가 남자에서만 3.1%로 나타났고, 가족기능도지수는 남녀별, 흡연군과 비흡연군간 통계적으로 유의한 차이가 있었다. 흡연자의 대부분(82.1%)은 금연을 고려하고 있었으며, 그 이유로는 건강문제가 제일 많았다. 결론 : 청소년들의 흡연율은 아직도 증가 추세에 있고, 점차 매일 흡연자의 빈도도 증가하고 있으며, 흡연행위를 정당하게 생각하는 경향이 두드러진다. 이에 따라 설문지에 의한 흡연율과 일산화탄소 측정기에 의한 흡연율을 비교하였으나 측정기의 여러 단점들로 인하여 일산화탄소 측정기에서 더 낮은 흡연율을 보였다. 그러므로 실제적인 흡연율 산출을 위해서는 보다 민감도와 특이도가 높은 흡연율 조사가 이루어져야 하겠다.
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박유준,배권호,진병수,정현진,박재신 대한비뇨의학회 2012 Investigative and Clinical Urology Vol.53 No.6
Purpose: The prostatic urethra is a bent tube, and the clinical significance of the prostatic urethral angle (PUA) was recently reported. We investigated the statistical significance of an increased PUA on the International Prostate Symptom Score (IPSS) in men with benign prostatic hyperplasia (BPH)/lower urinary tract symptom (LUTS). Materials and Methods: A retrospective analysis was done of 270 men with BPH/LUTS from July 2009 to June 2011. Prostate volume, PUA, and intravesical prostatic protrusion (IPP) were measured by transrectal ultrasonography (TRUS). The IPSS was analyzed separately as storage and voiding symptom score. In order to minimize the effect of prostate size on voiding, patients with prostate size over 40 ml were excluded. Results: The mean age was 62.0±9.3 years. The mean prostate volume was 29.0±5.5 ml (range, 20 to 40 ml), and median PUA and IPP were 34o (range, 12 to 52o) and 1.7 mm (range, 0 to 5.3 mm), respectively. The mean IPSS, mean IPSS-ss, and mean IPSS-vs were 19.0±8.2, 7.3±4.0, and 11.6±5.5, respectively. The prostate volume had no statistically significant correlation with IPSS, IPSS-ss, or IPSS-vs. IPP had a statistically significant correlation with IPSS (p<0.001), IPSS-ss (p<0.001), and IPSS-vs (p<0.001). PUA had no statistically significant correlation with IPSS or IPSS-ss. However, PUA had a significant correlation with IPSS-vs (p=0.047). Comparing a higher PUA (≥34o) with a lower PUA (<34o), patients with a higher PUA had a higher IPSS (p=0.001) and a higher IPSS-vs (p=0.001). There was no significant difference in IPSS-ss, prostate volume, or PSA between the two groups. Conclusions: IPP showed significantly correlated with the IPSS and voiding symptom score was affected by the PUA but not by the prostate volume. As the PUA increased, the patients' voiding symptoms worsened. Further study may be needed. Purpose: The prostatic urethra is a bent tube, and the clinical significance of the prostatic urethral angle (PUA) was recently reported. We investigated the statistical significance of an increased PUA on the International Prostate Symptom Score (IPSS) in men with benign prostatic hyperplasia (BPH)/lower urinary tract symptom (LUTS). Materials and Methods: A retrospective analysis was done of 270 men with BPH/LUTS from July 2009 to June 2011. Prostate volume, PUA, and intravesical prostatic protrusion (IPP) were measured by transrectal ultrasonography (TRUS). The IPSS was analyzed separately as storage and voiding symptom score. In order to minimize the effect of prostate size on voiding, patients with prostate size over 40 ml were excluded. Results: The mean age was 62.0±9.3 years. The mean prostate volume was 29.0±5.5 ml (range, 20 to 40 ml), and median PUA and IPP were 34o (range, 12 to 52o) and 1.7 mm (range, 0 to 5.3 mm), respectively. The mean IPSS, mean IPSS-ss, and mean IPSS-vs were 19.0±8.2, 7.3±4.0, and 11.6±5.5, respectively. The prostate volume had no statistically significant correlation with IPSS, IPSS-ss, or IPSS-vs. IPP had a statistically significant correlation with IPSS (p<0.001), IPSS-ss (p<0.001), and IPSS-vs (p<0.001). PUA had no statistically significant correlation with IPSS or IPSS-ss. However, PUA had a significant correlation with IPSS-vs (p=0.047). Comparing a higher PUA (≥34o) with a lower PUA (<34o), patients with a higher PUA had a higher IPSS (p=0.001) and a higher IPSS-vs (p=0.001). There was no significant difference in IPSS-ss, prostate volume, or PSA between the two groups. Conclusions: IPP showed significantly correlated with the IPSS and voiding symptom score was affected by the PUA but not by the prostate volume. As the PUA increased, the patients' voiding symptoms worsened. Further study may be needed.
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