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Quantitative evaluation of binding affinity between pancreatic cancer exosomes and lectins
박의선,김교범 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Exosomes are extracellular vehicles that contain a variety of proteins from cells. They can act as a potential biomarker for cancer detection due to the presentation of characteristic moieties originated from cells. Additionally, there is a higher frequency of polysaccharide on surface of exosomes. Thus, it could be suggested that lectins could bind with glycans expressed in exosomes as a substitute of conventional antibody. Thus, this study demonstrated the quantitative methods to determine the affinity between lectins and exosomes. The results indicate that Aleuria aurantia lectin (AAL) (binding with fucose) could bind strongly with exosomes compared with other lectins and has similar binding capacity to CD63 antibody. Furthermore, the PANC-1 exosomes could bind strongly with AAL as compared to those from other cells. In conclusion, AAL has a potential in detecting PANC-1 exosomes and further we will develop AAL-conjugated nanoparticles for diagnosis of pancreatic cancer.
Multiple Growth Factor Delivery for Skin Tissue Engineering Applications
박의선,김교범 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.6
Administration of exogenous growth factors (GFs) to a damaged site has been investigated for skin tissue regeneration. Among the many types of GFs and cytokines, epidermal growth factor, vascular endothelial growth factor, platelet-derived growth factor, fibroblast growth factor, and hepatocyte growth factor could be specifically used for stimulating molecules in wound healing as well as for recovery of damaged skin tissues. It is speculated that delivered GFs could stimulate various cellular functions, including proliferation, migration, deposition of extracellular matrix molecules, and remodeling of collagen synthesis. Although the physiological wound healing process is complex, engineering strategies for proper delivery of multiple therapeutic GFs could enhance the quality and quantity of regenerated skin tissues. As compared to single delivery of a GF, recent studies have proven that any combination of multiple GFs and/or therapeutic chemical factors synergistically facilitates the regeneration of damaged skin tissues. In order to maximize the stability, bioactivity, intrinsic therapeutic functionality, and efficiency of internal delivery of cargo GFs, it is essential to utilize tissueengineered biomaterials and related composites as implantable platforms. Successful fabrication and development of skin tissue engineering applications as well as subsequent surgical implantation of these platforms might provide clinical treatment for superior skin regeneration. Therefore, the present review summarizes the biological functions, related signaling mechanisms, and recent developments of tissue engineering applications for multiple GF delivery.
Park, Ui Sun,Choi, Kwan Sam,Kim, Kab-Sig,Kim, Nam Won,Choi, Kwang-Tae 충남대학교 생물공학연구소 1996 생물공학연구지 Vol.4 No.-
미트콘드리아내 유전자는 염기서열 재배치에 의해 단일 또는 여러 copy로 존재하는데 그 발현정도에 따라 생리적 특이 현상을 나타낼 것으로 생각되어 세포질 유전의 웅성불임현상에 대한 연구가 활발히 이루어져 왔다. 반음지성이며 생육이 느린 고려인삼(Panaxginseng C. A. Meye)의 특성을 유전자 수준에서 접근하기 위해 F_1ATPase α-subunit 유전자(aptA에 대해 실험하였다. 고려인삼에는 atpA 유전자가 미토콘드리아 DNA에 단일 copy로 존재함을 확인하였으며, 미토콘드리아 DNA의 SalI 및 PstI 유전자은행으로부터 Oenothera atpA유전자와 homology를 보이는 clony을 clone lift hybridization에 의해 선발하였는데, 각각 3.4 kb와 13 kb의 미토콘드리아 DNA조각을 함유하고 있었다. 이 Sal I clone과 Pst I clone에 대해 Southern blot hybridization과 부분적인 염기서열분석을 실시한 결과, 삽입된 3.4 kb와 13 kb의 미토콘드리아 DNA조각은 Oenothera, maize, pea, Phaseolus vulgaris의 atpA유전자와 homology를 보이는 sequence를 포함하고 있었다. 타 식물의 atpA 유전자의 coding sequence와 비교해 볼때, Sal I clone은 initiation codon으로부터 약 300 bp를 제외한 atpA유전자의 나머지 약 1.2 kb sequence를 포함하고 있고, Pst I clone은 atpA유전자의 완전한 coding sequence를 포함하고 있는 것으로 여겨진다. 이들 두 clone의 제한효소 지도와 부분적인 염기서열이 제시되었다. 고려인삼은 단일 copy의 atpA유전자를 갖고있기 때문에 이 타 식물에서와 같이 복잡한 유전자 재배치에 따른 생리적 불균형의 가능성은 없는 것으로 사료된다. Molecular cloning and restriction mapping on ATPase α-subunit gene (atpA) were carried out to obtain genomic information concerned with the gene structure and organization in Korean ginseng mitochondria. Two different clones containing the homologous sequence of atpA gene were selected from SalI and PstI libraries of mitochondrial DNA (mtDNA) of Korean ginseng. The sizes of mtDNA fragments inserted in SalI and PstI clones were 3.4 kb and 13 kb, respectively. Southern blot analysis with [^32P]labelled Oenothera atpA gene probe showed that atpA gene sequence was located in 2.0 kb XbaI fragment in PstI clone and in 1.7 kb XbaI fragment in SalI clone. A partial sequencing ascertained that the SalI clone included about 1.2 kb fragment from SalI restriction site to C-terminal sequence of this gene but about 0.3 kb N-terminal sequence of open reading frame was abscent. The PstI fragment was enough large to cover the full sequence of atpA gene. The same restriction pattern of the overlapped region suggests that both clones include the same fragment of atpA locus. Date of Southern blot analysis and partial nucleotide sequencing suggested that mtDNA of Korean ginseng has a single copy atpA gene.
김영국,박의선,박범준,김교범 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.5
Exosomes of human mesenchymal stem cells (hMSC) are known to effectively increase the proliferation rate of chondrocytes and stimulate cartilage extracellular matrix. However, the therapeutic efficacy of the other signaling direction (i.e., the effect of chondrocyte-derived exosomes on hMSC) has not been extensively investigated. Therefore, the present study was designed to investigate exosome-mediated in vitro bidirectional signalings between progenitor hMSC and mature chondrocytes in cartilage tissues with various culture medial formulations. The exosomes isolated from bovine chondrocytes (BC) and hMSC (50 μg/ mL of exosomes per 3000 cells) were treated to hMSC and BC, respectively. Both cells were cultured in media formulations with 10% FBS, 10% exosome free-FBS, and 0.5% FBS. A variety of cellular responses by exosome treatments including proliferation, chondrogenic differentiation, cartilage extracellular matrix (ECM) deposition were evaluated using WST-1 assay, RT-PCR, and alcian blue staining for glycosaminoglyca (GAG) content. The results demonstrated that bidirectional exosome treatments increased proliferation of both BC and hMSC, and similar bidirectional influences including chondrogenic differentiation, glycosaminoglycan (GAG) ECM deposition were also up-regulated in both cell populations. Moreover, exosome-mediated in vitro activation between two cell populations could be regulated by media formulations. Therefore, exosomes could play important signaling roles in communication between two major cell populations in cartilage tissues.
액화질소 초저온과 이원냉동 초저온 냉열의 비교 실험적 연구
김철수(Chul-Su Kim),박의선(Eui-sun Park),이상철(Sang-Chul Lee),김영근(Yeong-Geun Kim),이병호(Byung-Ho Lee),정한식(Han-Shik Chung),정효민(Hyo-Min Jeong) 한국동력기계공학회 2006 한국동력기계공학회 학술대회 논문집 Vol.- No.-
This paper represents the cold energy for liquefied nitrogen gas and cascade refrigerator. In this study, the vaporizer of liquefied nitrogen gas has the fin coil tube type with the dimension of inside diameter of 10㎜ and outside diameter of 12㎜. Also, the total length of vaporizer is 20,000㎜. The main experimental parameters are the mean velocity in duct and the supplied flow-rates of liquefied nitrogen gas. For the cascade refrigeration system, the refrigerants are ethane(R-170) in the high pressure stage and R-22 in the low pressure stage.