삼차원 T형강체를 이용한 편심코어를 가진 전단벽-골조 구조물의 효율적인 지진해석모델 개발

박용구,이동근,김현수,Park, Yong-Koo,Lee, Dong-Guen,Kim, Hyun-Su 한국전산구조공학회 2013 한국전산구조공학회논문집 Vol.26 No.1

전단벽-골조 구조시스템의 구조적인 거동은 휨거동하는 전단벽과 전단거동하는 골조의 상호작용에 의하여 결정된다. 이러한 전단벽-골조 구조물의 거동특성을 효과적으로 고려하기 위하여 선행 연구에서는 2차원 T형 강체를 사용한 단순 해석모델을 제안하였다. 본 논문에서는 이를 바탕으로 편심코어를 가진 전단벽-골조 구조물에 대한 효율적인 해석모델을 제안한다. 2차원 등가모델을 3차원으로 확장하여 비틀림 거동을 고려할 수 있도록 하였고, 그 결과 제안하는 등가모델이 편심코어를 가지는 전단벽-골조 구조물에도 적용가능 하도록 하였다. In a shear wall-frame structural system, the structural response is determined by the interaction between the shear wall in bending mode and the frame in shear mode. In order to effectively consider these characteristics of a shear wall-frame structure, the simplified numerical model using the T-shape rigid body was suggested in the previous study. Based on the previously proposed model, an efficient numerical model for a wall-frame structure with an eccentric core has been proposed in this study. To this end, the previously proposed 2D model is extended to the 3D model and it is enhanced by considering torsion effects. As a result, the enhanced model can be applied to the analysis of a wall-frame structure with an eccentric core as well as a centric core.

Isolation and Culture of Mesophyll Protoplasts from in vitro Cultured Populus alba × P . glandulosa

박용구,한경환 ( Young Goo Park,Kyung Hwan Han ) 한국산림과학회 1986 한국산림과학회지 Vol.73 No.1

N/A This study was carried out to investigate the optimum conditions for isolation and culture of mesophyll protoplasts from Populus alba × P. glandulosa. The results obtained from the experiments are as follows; 1) The suitable concentration of BAP for shoot multiplication was 0.4 ㎎/ℓ. 2) High yield and viability of isolated protoplasts were obtained by our high enzyme-short time incubation method. 3) Optimum enzyme concentrations for mesophyll protoplast isolation were Cellulase 2%, Macerozyme 0.8%, Hemicellulase 1.2%, Driselase 2%, and Pectolyase Y-23 0.05%. 4) 0.6M mannitol in enzyme solution was the most effective for protoplast isolation and viability. 5) The most adequate pH level of enzyme solution was pH 5.6. 6) The effect of DTT and MES buffer was significant. 7) For protoplast purification, 0.6M sucrose was the most proper concentration. 8) The adding effect of Dextran T40 in floating solution was important. 9) The mesophyll protoplasts isolated through our high enzyme-short time incubation method revealed successful response to culture condition over 3 weeks of culture.

몇가지 삼림수종 (森林樹種) 종자발아에 (種子發芽) 대한 낙엽송엽 (落葉松葉) 추출물의 Allelopathic 효과

박용구,강군수,신동일 ( Young Goo Park,Goon Su Kang,Dong Ill Shin ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.1

Inhibitory effect of L. leptolepis fallen leaf extracts on the germination of Pinus densiflora, × Pinus rigitaeda, Pinus rigida, Pinus thunbergii and Larix leptolepis was investigated. Germination of those seeds in culture room and in pot at field showed the highly significant inhibitory effects by the aqueous extracts from L. leptolepis fallen leaves. Among them L. leptolepis was the most severely inhibited by the aqueous extracts used. To identify allelopathic substances, thin-layer chromatography was employed. Gallic, ferulic, t-cinnamic and vanillic acids were identified from fallen leaves of L. leptolepis. From the results, it is assumed that autotoxicity of L . leptolepis may act as inhibitory factors on germination of the species in natural stands.

회음부를 침범한 근위형 유상피육종 - 증례보고 -

박용구,박호철,Park, Yong-Koo,Park, Ho-Chul 대한근골격종양학회 2001 대한골관절종양학회지 Vol.7 No.2

Epithelioid sarcoma has been well characterized as a distincitve entity. In the classic form, it occurs in the distal extremities of young adults. However, in the proximal form, it was found mostly in the pelvis, perineum, and genital tract of young to middle-aged adults. Morphologically, the tumor cells had promient epithelioid or rhabdoid features with marked cytologic atypia, and grew in a multinodular pattern. Immunohistochemically, cytokeratin, epithelial membrane antigen, and vimentin were positive in the tumor cells. This reports dealt with 32-year-old male lesion on his perineum. The tumor shows typical features of proximal type epithelioid sarcoma. We also discussed pathological differential diagnosis and prognosis on this particular disease. 유상피육종은 전형적인 형태인 경우에는 비교적 젊은 사람의 손이나 발과 같은 말단부에 호발하는 종양이다. 그러나 근위형의 경우는 주로 청장년층의 골반, 회음부 또는 생식기에 발생한다. 형태학적으로 종양세포는 뚜렷한 유상피 형태를 취하거나, rhabdoid형태를 보이며, 심한 세포학적 이형증과 함께, 다결절 형태로 성장한다. 면역조직화학적 검사에서는 특징적으로 cytokeratin, epithelial membrane antigen, vimentin이 양성이다. 본 보고는 32세 남자의 회음부에 발생한 근위형 유상피육종을 기술하였다. 형태학적으로 전형적인 근위형 유상피 육종의 조직학적 소견을 관찰하였으며, 병리조직학적 감별점과, 환자의 예후에 관하여 토론하였다.

우리나라 양봉산업지원 관리부서의 일원화를 촉구하며

박용구,Park, Yong-Gu 한국양봉협회 2008 양봉협회보 Vol.330 No.-

Immunohistochemical c-fos Expression in Osteosarcoma

박용구,박혜림,Park, Yong-Koo,Park, Hye-Rim The Korean Musculoskeletal Tumor Society 1999 대한골관절종양학회지 Vol.5 No.3

c-fos와 c-jun은 암 유전자의 하나이며, 이 유전자의 단백질 산물은 여러 가지의 다른 활성화 단백 (activator protein 1, AP-1)으로 골 종양에서 골세포의 증식과 분화를 조절하는 중요한 역할을 하는 인자 중 하나로 알려져 있다. 본 연구에서는 단클론 항체를 이용하여 포르말린에 고정된 파라핀 포매조직을 이용하여 35례의 사람 골육종에서 c-fos 단백의 발현을 연구하였다. c-fos의 발현은 골 형성 병변에서 주로 발현되며, 저등급의 연골형성 병변에서는 발현이 관찰되지 않았다. 높은 빈도의 c-fos 단백의 발현이 골아성 골육종에서 발현되었으나 (17례 중 13례에서 1등급 내지 2등급으로 발현), 2례의 연골형성 골육종, 1례의 섬유아세포성 골육종, 2례의 방골성 골육종에서는 음성으로 나타났다. 2례의 혈관확장성 골육종에서는 양성으로 c-fos 단백의 발현되었다. 비록 조직학적으로 고등급의 골육종에서 면역조직화학적 염색상 고 빈도의 c-fos 단백의 발현이 관찰되나, 조직학적 등급과, 면역염색상 발현사이에 통계적인 유의성은 관찰되지 않았다. 이상의 결과로 c-fos 단백이 골육종의 발생에 관여할 것으로 추론되며, 저등급의 연골형성 육종에 이 단백의 역할에는 추후 연구가 필요할 것으로 사료된다. The products of c-fos and c-jun proto-oncogenes form the heterodimeric complex activator protein 1 (AP-1), which plays an important part in the control of bone cell proliferation and differentiation, as well as in the development of bone tumors. The expression of c-fos protein was examined in 35 cases of human osteosarcomas as formalin-fixed paraffin-embedded tissue sections using a monoclonal antibody. The expression of c-fos was restricted to bone-forming lesions, while low grade cartilaginous tumors were devoid of immunoreactivity. The highest levels of c-fos expression were detected in osteoblastic osteosarcoma (13 of 17 cases with grade one on two) while two chondroblastic osteosarcomas, one fibroblastic osteosarcoma, and two parosteal osteosarcomas were negative. Two cases of telangiectatic osteosarcomas were positive for c-fos protein. However, since there is a tendency of high c-fos protein expression at the higher histological grade, significant differences were not present in the expression of c-fos protein. Thus c-fos expression may be implicated in the development of osteosarcomas, but they appear to have little or no relevance in the development of low grade cartilaginous neoplasms.

현사시나무 기내배양 엽육조직에서 분리된 원형질체 배양 및 식물체 재분화

박용구,손성호 ( Young Goo Park,Sung Ho Son ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.2

The leaf mesophyll protoplasts of Populass alba × glandrdosa were isolated from leaf of plantlet in vitro and cultured for plant regeneration. The MS medium (minus NH₄NO₃) with 0.5 ㎎/ℓ BAP and 2.0 ㎎/ℓ 2, 4-D showed the moderate frequency of dividing protoplasts cultured by the lipuid plating method during the first week of culture. The percentage of colony formation was revealed the highest frequency by the gauze contained semi-solid agar plating method after 5 weeks cultured. Ridding out the gauze, the micro-callus was formed on the same semi-solid medium in 8 weeks after protoplasts culture. For proliferation of callus, mini-callus was transferred on the MS solid medium with 0.5 ㎎/ℓ 2, 4-D and 0.1 ㎎/ℓ BAP 12 weeks after culture. Shoot regeneration occurred when the calli derived from protoplasts were cultured on MS medium with 1.0 ㎎/ℓ zeatin and such shoots could be readily rooted on the one half strengthen MS medium with non-phytohormone. Rooting shoots were planted in green-house 22 weeks after protoplast culture.

회원작품 - 순창 금과어린이집

박용구,Park, Yong-Gu 대한건축사협회 2016 建築士 Vol.2016 No.8

수원포플러와 구아디 포플러 원형질체(原形質體) 융합(融合)에 의한 체세포잡종체(體細胞雜種體) 유도(誘導)

박용구,김정희,손성호,Park, Young Goo,Kim, Jung Hee,Son, Sung Ho 한국산림과학회 1992 한국산림과학회지 Vol.81 No.3

유망(有望) 속성수(速成樹)로 개발중(開發中)인 수원포플러(P. koreana ${\times}$ nigra var. italica)와 포플러 낙엽병(落葉病)에 내성(耐性)을 가진 구아디포플러(P. euramericana cv. Guardi)의 엽육조직(葉肉組織)에서 원형질체(原形質體)를 분리융합(分離融合)하여 잡종식물체(雜種植物體)를 생산(生?)하였다. 실험재료(實驗材料)인 수원포플러는 BA $0.5{\mu}M$, 구아디포플러는 BA $2.0{\mu}M$ 처리(處理)된 MS 배지(培地)에서 대량(大量) 증식(增殖)시킨 후 1/2 MS배지에서 계대배양(繼代培養)하여 전개(展開)된 엽조직(葉組織)을 사용(使用)하였다. 수원포플러는 효소용액(酵素溶液) I (Cellulase 2.0%, Macerozyme 0.4%, Hemicelluase 1.2%, Driselase 2.0%, Pectolyase 0.05%)에서 구아디포플러는 효소용액(酵素溶液) II (Cellulase 1.0%, Macerozyme 0.4%, Hemicellulase 1.2%, Driselase 2.0%, Pectolyase 0.05%)에서 원형질체(原形質體)를 분리(分離)하여 각각 $4.04{\times}10^7$, $2.45{\times}10^7$개의 높은 수율(收率)을 얻었다. 양수종간(兩樹種間)의 원형질체(原形質體) 융합율(融合率)은 PEG 40%가 포함(包含)된 융합용액(融合溶液)에 20분 처리(處理)하고 $Ca^{2+}$ 30mM 첨가(添加)된 희석액(稀釋液)(pH 10.5)을 사용(使用)하였을때 양수종간(兩樹種間) 1:1 융합율(融合率)이 30%정도로 높게 나타났다. 융합(融合)된 원형질체(原形質體)는 0.6M sucrose, $4.5{\mu}M$ 2, 4-D, $0.5{\mu}M$ BA가 첨가(添加)된 8p-KM에서 정치(定置) 혹은 진탕배양(震湯培養)하여 2개월후 캘루스를 얻을 수 있었으며, $5.0{\mu}M$ zeatin 처리구(處理區)에서 평균 4개의 식물체(植物體)가 재분화(再分化)되었다. 융합산물(融合?物)에서 유래(由來)한 식물체(植物體)의 잡종성(雜種性) 여부(與否)를 확인(確認)하기 위해 SDS-PAGE를 실시(實施)하였다. 모수(母樹)인 수원포플러와 구아디포플러간에는 단백질형에 차이(差異)가 있었으며 재분화(再分化) 개체중(個體中) 양친(兩親)의 중간형(中間型)을 나타내는 개체(個體)는 세포융합(細胞融合)에 의한 재분화개체(再分化個體)로 추정(推定)할 수 있었다. Protoplasts isolated from leaf mesophyll tissues of Populus koreana ${\times}$ P. nigra var. italica were fused with those of P. euramericana cv. Guardi. Well expended healthy leaves of 5 to 7 week-old-plantlet grown in vitro were used as source materials. Leaves from P. koreana ${\times}$ P. nigra var. italica and P. euramericana cv. Guardi were digested in enzyme solution I (2.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v) and enzyme solution II (1.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v), respectively, The highest frequency of fusion among the protoplasts originated from the two source materials was approximately 21% using 40% PEG or 15% dextran. In addition, fusion frequency was enhanced by incorporating 30mM of $Ca^{2+}$ in eluting solution at pH 10.5. Dividing cells and/or mint-calli were obtained by culturing the fusion products in a liquid 8p-KM medium supplemented with 0.6M sucrose, $0.45{\mu}M$ 2, 4-D, and $0.5{\mu}M$ BA. Shoots were regenerated from the fusion product-derived calli after culture on MS medium containing $5.0{\mu}M$ zeatin. To verify the putative hybrid or cybrid, SDS-PAGE was carried out. From the 24 regenerants, just two plants showed intermediate protein band patterns compared with those of the original source plants.

잣나무의 기내배양에 관한 연구 (Ⅰ) - Callus 의 유발과 생장 -

박용구,김우룡 ( Young Goo Park,Oue Ryong Kim ) 한국산림과학회 1983 한국산림과학회지 Vol.59 No.1

Embryo tissue from Pinus koraiensis was established in culture on G.D. and W.S. basal medium supplemented with the auxin (NAA, 2,4-D, IBA) and kinetin. The various combinations of growth regulators were studied in order to determine the specific requirements of the callus tissue in vitro. The inorganic nutrient combination of G.D. medium was found to be better than that of W,S. medium. G,D. basal medium supplemented with 0.1 ppm N.AA and 0.1 ppm kinetin was the most successful nutrient combination for the growth of callus induced from the embryo of P. koraiensis.