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      • KCI등재

        능이의 성분에 관한 연구 (제1보)

        박완희 한국균학회 1983 韓國菌學會誌 Vol.11 No.2

        The aims of this study were to investigate various components and their physiological activities of Sarcodon aspratus (Berk.) S. Ito which grows wildly in Korea, belonging to the family Thelephoraceae. The analysis of the powered carpophore of this fungus by TLC and an amino acid autoanalyzer revealed that it contained twenty-one free amino acids and that twenty-two total amino acids were identified in its acid hydrolysate. These amino acids were also quantified.

      • KCI등재
      • KCI등재

        한국산 고등 균류의 효소에 관한 연구 (1) : 능이의 단백질 분해효소의 확인 Identification of Protease in Sarcodon aspratus

        박완희 한국균학회 1985 韓國菌學會誌 Vol.14 No.1

        The purposes of this study were to investigate enzyme components and its physiological activities of Sarcodon aspratus (Berk.) S. Ito which grows wildly in Korea, belonging to the family Thelephoraceae. The carpophores of the fungus was extracted with cooling distilled water and salted out by ammonium sulfate. The precipitate was purified by dialysing through visking tube against distilled water and then dissolved with pH 7.8 ammonia aqua, and the extract was filterated. The fraction of filtrate was obtained as light brown powder after lyophilization and determined proteolytic activity. Protease activity of Sarcodon aspratus (Berk.) S. Ito was about two-third of that of pepsin on casein by cup method. The proteolytic potency of this enzyme was found to be 500 unit/㎎. This proved the efficacy of the mushroom when it was used as a folk medicine for treating indigestion of beef.

      • KCI등재

        싱싱회류 생산업체의 HACCP 시스템 구축 전 후의 미생물학적 평가

        박완희,이성학,정덕화 한국식품위생안전성학회 2004 한국식품위생안전성학회지 Vol.19 No.2

        Raw-fish food contains a lot of moisture and is a high-protein food. It is a first-stage processed food taking a lot of manual work. Therefore, it is classified as a PHF food, very liable to cause a bacterial food-poisoning. But its manufacturers are usually small-sized and a systematic sanitation management is difficult to expect. But the manufacturer participating in this study produces chilled fresh raw-fish food. Fish are sliced into two fillets, which are packaged under vacuum, kept and distributed in refrigerators, and sold within a day. It is a newly-developed kind of raw-fish food, and a more improved kind of raw-fish food making possible a systematic sanitation management. The HACCP (Hazard Analysis and Critical Control Point) is a systematic and continuous process-control method which is very efficient for controling food sanitation and reducing the expenses. A new HACCP model has been developed to be applied to a large-sized chilled fresh raw-fish food manufacturer. To ascertain its efficiency, the baterial examination was done to its workplace and products. The significance test was done on its data by "SPSS 12.0 for Window" and "Mann-Whitney U Test". The numbers of bacteria on its final products were significantly different in flatfish and porgy. The number of bacteria tended to decrease in each time-differential sampling (P<.00l). The final food products showed no food-poisoning bacteria in all the time-differential tests and in all the samplings, which proves that the CCP of the HACCP system is under control. After the SSOP program was applied, no pathogenic bacteria were found in the work-place, and the kinds and numbers of bacteria decreased. The numbers of general bacteria and colon bacilli also showed a significant difference from those before the SSOP program in the filleting board (P<.05), in the skinning board (P<.0l), in the neck-removing knife (P<.05), and in the filleting knife (P<.01). The working equipments, periodically disinfected, also showed a significant difference in sanitary conditions (in the dehydrator, P<.05). The number of bacteria found on the food-touching surface was within the standard (below 500/l00 cm$^2$) After the SSOP program was applied, the general bacteria and colon bacilli were not found. The quality of water used in the food processing was also within the standard. The numbers of bacteria falling from the air in the work-place were negligible in all the samplings (<30CFU/l000ι). The staphylococci and fungi were not found.

      • 葡萄酒酵母의 分離 및 同定에 關한 硏究

        박완희 진주산업대학교 1977 論文集 Vol.15 No.-

        晋州 地方에서 재배되고 있는 葡萄 中에서 葡萄酒 생산에 가장 적합한 品種을 선택하기 위해서 각 葡萄의 成分 分析을 했으며 또한 포도에 自然的으로 存在하는 醱母를 分離, 同定한 결과는 아래와 같다. 1. 葡萄의 成分 分析 結果 Delaware와 Muscat Bailey A는 糖 함량이 높아서 自然 醱酵시켜 葡萄酒를 만들 수 있고 그외의 것은 포도주 제조를 위해서는 加糖處理가 필요하였다. 2. 분리 株中, M-5가 가장 醱酵力이 우수하였다. 3. 선정된 菌株 M-5를 同定한 結果 Saccharomyces chevalieri와 일치하였다. In order to select the suitable variety for wine making in Jin Ju area, the composition of the each grape juice was analyzed. Also the yeasts existing natually on the grape were isolated and one strain of them selected and screened. The results obtained were as follows. 1. The reducing sugar content was high in Delaware and Muscat Bailey A, which could be fermented naturally, but the other varieties needed chaptalisation for wine making. 2. Among the isolated 21 strains, M-5 showed the strongest fermentative ability. 3. The selected strain, M-5 was screened and identified as Saccharomyces chevalieri.

      • 前ㆍ後期 分割募集의 妥當性 檢證 : 1988학년도 慶星大學校 入學生을 對象으로

        朴玩熙 慶星大學校 1990 論文集 Vol.11 No.3

        The purpose of this study is to investigate the validity of the former-latter term divide invitation in entrance examination for a univrsity by analyzing the change of school register, the adaptation and affection in college life, the scores of entrance examination and university grades. As the sample for this study, 555 college students attended in the school year, 1988 were selected according to the judgement sampling method in Kyunsung University. The materials used for this study are the change of school register, the scores of entrance examination and university grades. The tool is the questionnaire made by the researcher, which is concerning with the adaptation and affection in college life. The statistical methods used in this study are the percentage, the Pearson's product moment correlation, and ANOVA techniques with the SPSS programs. Major findings of this study are sd follows; 1) Considering the change of school register, the former-latter term divided invitation is valid in College of Law and Political Science, College of Commerce and Economics, and College of Pharmacy but it is not valid in College of Liberal Arts, College of Science and College of Engineering. 2) There are a few differences between the former group and the latter group in the adaptation and affection in Collage life but these are not much significant in statistical level. 3) The scores of unofficial report of the latter group are higher than those of the former group and those difference are significant in statistical level. 4) The coefficients of correlation between the scores of unofficial report and the university grades are higher than those of the scores of government entrance examination and the university grades. In other words, the scores of unofficial report more correctly predict the university grades than the scores of government entrance examination. 5) The coefficients of correlation between the achievements of the cultural subjects and the scores government entrance examination or unofficial report are higher than those of the achievements of the major subjects and the scores of the government entrance examination or unofficial report. In other words, being promoting to higher school year, the predictability of the score the entrance examination to the university grades is low. 6) The university grades of the latter group are higher than those of the former group, and differences between the two groups are sigificant in statistical level.

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