폐암 세포주 NCI-H1373의 염색체 이상 분석

박수연(Soo-Yeun Park) 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.2

종양에서 흔히 보이는 염색체 이상에 대한 세포유전학적 분석은 많은 질병의 진단과 예후를 판단하는데 중요한 부분이 되어 왔다. 그러나, 고형암에서는 분석 가능한 염색체를 얻기가 어렵고 많은 염색체 이상을 동반하고 있어, 불균형적 염색체 재배열을 통상적인 세포유전학적 방법만으로는 구분하기가 매우 어렵다. 비교 유전체 보합법(comparative genomic hybridization, CGH)은 통상적인 세포유전학적 분석으로는 용이하지 않았던 전 염색체 상에서의 상대적인 염색체의 변화를 한번에 알 수 있게 하는 빠르고도 효과적인 방법이다. Array-CGH는 DNA copy 수의 변화뿐 아니라, 유전자의 증가나 감소를 직접 알 수 있는 방법으로, 그 해상력은 기존의 CGH에 비하여 높다. 이 연구에서는 핵형분석을 통하여 이미 그 염색체 이상을 보고한 폐암 세포주 NCI-H1373에 대한 CGH를 시행함으로써, CGH의 정확성과 일차 검색 방법으로서의 유용성을 확인하고자 하였으며, array-CGH를 통하여 해상력을 높일 수 있음을 규명하고자 하였다. 그 결과, NCI-H1373에 대한 CGH 및 array-CGH 결과는 기존에 핵형 분석에서 보고한 염색체의 증감과 일치하였다. 또한, array-CGH의 결과는 CGH의 결과보다 좁혀진 범위에서 나타나게 됨을 알 수 있었다. 이와 같은 결과로, CGH와 array-CGH는 암에서 발생하는 염색체 변화를 선별하는데 매우 유용한 방법임을 확인하였다. The cytogenetic analysis of recurring chromosomal aberrations play an important part to decide pathogenesis and prognosis of cancers. However, due to difficulties culturing tumor cells and complexity associated with the lesions, routine cytogenetic studies to analyze chromosomal imbalances are not sufficient. Comparative genomic hybridization (CGH) is a fluorescence in situ hybridization (FISH) technique to identify genomic imbalances in cancers, and array-CGH provides a method to measure the DNA copy-number changes quantitatively at an extremely high resolution and to map them directly onto the complete linear genome sequences. The purpose of this study was to confirm the utility of the CGH and array-CGH in analyzing chromosomal aberrations in lung cancer cell line, NCI-H1373, which was previously analyzed by karyotype analysis. The results of CGH and array-CGH in NCI-H1373 were similar to karyotype analysis. The array-CGH allowed us to pinpoint regions that were gained and lost. In this study, it was confirmed that CGH and array-CGH are an useful screening technique to analyze chromosomal aberrations in tumors.

수용액 중 OrangeⅡ 흡착 제거를 위한 우모폐기물의 이용가능성

박수연 ( Soo-yeun Park ),유지연 ( Ji-yeon Yoo ),손홍주 ( Hong-joo Son ) 한국환경과학회 2018 한국환경과학회지 Vol.27 No.9

The objective of this study was to investigate the adsorption potential of chicken feathers for the removal of OrangeⅡ (AO7) from aqueous solutions. Batch experiments were performed as a function of different experimental parameters such as initial pH, reaction time, feather dose, initial OrangeⅡ concentration and temperature. The highest OrangeⅡ uptake was observed at pH 1.0. Most of the OrangeⅡ was adsorbed at 2 h and an adsorption equilibrium was reached at 6 h. As the amount of chicken feather was increased, the removal efficiency of Orange II increased up to 99%, but its uptake decreased. By increasing the initial concentration and temperature, OrangeⅡ uptake was increased. The experimental adsorption isotherm exhibited a better fit with the Langmuir isotherm than with the Freundlich isotherm, and maximum adsorption capacity from the Langmuir constant was determined to be 0.179244 mmol/g at 30℃. The adsorption energy obtained from the Dubinin-Radushkevich model was 7.9 kJ/mol at 20℃ and 30℃ which indicates the predominance of physical adsorption. Thermodynamic parameters such as △G⁰, △H⁰, and △S⁰ were -12.28 kJ/mol, 20.64 kJ/mol and 112.32 J/mol K at 30℃, respectively. This indicates that the process of OrangeⅡ adsorption by chicken feathers was spontaneous and endothermic. Our results suggest that as a low-cost biomaterials, chicken feather is an attractive candidate for OrangeⅡ removal from aqueous solutions.

18주 태아 뇌에서 발현하는 FB174 cDNA의 유전체 DNA 분리 및 염색체 위치

박수연(Soo Yeun Park),강윤희(Yoon-Hee Kang),이희래(Hee Lai Lee),김 현(Hyun Kim),박선화(Sun-Hwa Park) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.6

최근 사람 유전체 사업의 진행으로 수많은 유전자들이 발굴되고 다양한 실험방법들이 개발되고 있으나 발굴한 유전자들 의 기능을 예측하고 염색체 위치를 결정하는 것은 용이하지 않다. 이 연구에서는 태아의 뇌 형성이 활발한 시기인 태생기 18주의 뇌로부터 cDNA probe pool을 작성하고, 염기서열을 분석 하였다. 이 중에서 FB174를 선택하여 흰쥐 조직 절편에 대한 in situ hybridization 조직 화학법을 시행하여 그 발현양상을 관찰하였다. 염색체 위치를 규명하기 위하여, 사람 유전체 서고 검색을 시행하여 사람 유전체 DNA를 분리하였고, 이 유전체 DNA를 biotin으로 표지한 후 형광 in situ hybridization을 시행하였다. 서열분석과 GenBank 자료분석에서 FB174 클론은 사람염색체 13q14에 위치한다고 알려진 번역 수준에서 조절되는 암 단 백질(TCTP)과 매우 유사한 것으로 나타났다. FB174 클론의 mRNA를 흰쥐 조직 절편에 in situ hybridization 조직 화학법을 시행한 결과 발현을 관찰할 수 없었다. 분리된 FB174 클론의 유전체 DNA로 형광 in situ hybridization 시행한 결과 염색체띠 7q22에 위치함을 확인하였다. 이상의 결과에서 FB174 유전자는 기존에 알려진 TCTP 유전자의 서열과 매우 높은 유사성을 고려할 때 그와 관련된 새로운 유전자일 것으로 추정된다. Eventhough surmountable amounts of genes are being cloned and a number of methods are being developed by human genome project, it’s not easy to predict possible functions of genes and determine the chromosomal locations of genes. In this experiment, cDNA pool was made from 18 weeks old human fetal brain and analyzed the sequences. FB174 clone was chosen, in situ hybridization histochemistry was performed on developing and adult rat tissue section to observe the tissue specificity and developmental expression of this gene. To observe the chromosomal location of FB174 clone, the genomic DNA from human genomic library was isolated and fluorescence in situ hybridization was carried out. By sequencing and sequence search with GenBank data it was revealed that cloned FB174 cDNA was quite similar to translationally controlled tumor protein which is known to locate to human chromosome 13q14. The expression of FB174 mRNA was not detected in rat tissue sections by in situ hybridization histochemistry. Fluorescence in situ hybridization using biotin labeled FB174 probe resulted in specific labeling of human chromosome 7q22. These results and high sequence homology of FB174 to known translationally controlled tumor protein suggest that FB174 clone may be a new translationally controlled tumor protein-related gene.

Effects of p27 Overexpression on Head and Neck Squamous Cell Carcinoma Cell Lines

박수연(Soo-Yeun Park),이동욱(Dong Wook Lee) 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.3

p27은 cyclin-dependent kinase inhibitor (CKI)로 알려져 있지만 종양 억제 유전자로서도 주목을 받고 있다. 종양이 생성되는 과정에서 p27 단백질의 발현은 감소하는 것으로 알려져 있으며, 여러 종양에서 p27의 발현의 감소는 불량한 예후 인자로 작용하고 있다. 본 연구에서 저자들은 재조합 adenovirus-p27 (ad-p27)을 제작하였고, 이 ad-p27의 두경부 편평상피암 세포에 대한 항암효과를 세포 성장의 억제, 세포주기의 정지, 세포고사의 유도의 측면에서 조사하였다. 두경부 편평상피암 세포주에 ad-p27을 이입한 결과 p27의 과발현이 실험한 모든 세포주에서 관찰되었다. 그리고 p27의 과발현에 의하여 의미있는 암세포의 성장 억제, 세포주기의 정지 및 세포고사의 증가가 유도되었다. 이러한 실험 결과에서 저자들은 p27의 과발현이 두경부 편평상피암 세포주에서 항암효과를 나타낼 수 있다는 결론을 유추할 수 있었으며, p27 유전자 이입을 통하여 암세포 내에 p27 단백질을 증가시켜 줌으로써 두경부 편평상피암을 비롯한 여러 종양의 치료 및 예방이 가능하리라고 생각하였다. p27 is a cyclin dependent kinase inhibitor and plays an important role in negative regulation of the cell cycle. It has been revealed that down-regulation of p27 is frequently found in various cancers and is well associated with their malignancies including poor prognosis. In the present work, we constructed a recombinant adenovirus-p27 (ad-p27), and investigated its antitumor effects including inhibition of cell proliferation, arrest of cell cycle, and induction of apoptosis in head and neck squamous cell carcinoma (HNSCC) cells. On transduction of the HNSCC cell lines with ad-p27, overexpression of p27 was confirmed in all cell lines tested. In addition, overexpression of p27 resulted in significant inhibition of cancer cell proliferation with increased apoptosis and cell cycle arrest in HNSCC cell lines. From these results, it was suggested that overexpression of p27 could show anti-tumor effects in HNSCC cell lines and it is possible that an increase of p27 protein by introducing the p27 gene into human cancer cells may be helpful for the treatment or prevention of human cancers, especially HNSCC.

소아의 장중복증

박수연(Soo Yeun Park),박진영(Jin Young Park) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.75 No.4

Purpose: Intestinal duplication is a rare congenital anomaly that may be found anywhere from the mouth to the anus. The clinical presentation varies depending on the anatomic location or the size of the duplication, and the presence of the ectopic gastric tissue. The aim of this study was to analyze the clinical characteristics, the diagnostic and therapeutic methods, the location, the anatomic type and the associated anomalies of intestinal duplication. Methods: We reviewed the medical record of eighteen patients with duplication of the alimentary tract and these patients had been treated at Kyungpook National University Hospital from July 1995 through October 2007. Results: There were 9 boys and 9 girls. Their ages ranged from 3 days to 15 years. The most common symptom was abdominal pain. The duplicated segment acted as a leading point of intussusception in 3 cases. Two patients had melena. The duplications were cystic in fifteen cases and three others were tubular. In twelve cases, the lesions were located in the ileum; two other cases were located in the anal canal, one other was located in the in the transverse colon, another was located in the transverse and descending colon, one was located in the in the duodenum and one was located in the jejunum. Ultrasonography, computed tomography and barium enema were helpful as diagnostic tools. Three cases were diagnosed by prenatal ultrasonography. Associated anomalies were documented in four cases. All the patients underwent surgery. A duplicated lesion was lined with ectopic gastric mucosa in only one case. One patient still suffers from short bowel syndrome as a result of volvulus. Conclusion: The early operative treatment of intestinal duplication is safe and effective to prevent complications. In patients with intussusception, duplication should be considered as one of the causes of the leading point of intussusception.

비교 유전체 보합법에 의한 두경부 편평상피세포암 세포주의 염색체 이상 분석

박수연(Soo-Yeun Park) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.6

최근 종양에서 흔히 보이는 염색체 이상에 대한 연구가 주목받고 있으며, 이러한 연구는 암의 진단법 개발과 예후 결정에 중요하다. 비교 유전체 보합법(comparative genomic hybridization, CGH)은 암 조직의 모든 염색체 상에서의 염색체 변화를 한 번에 알 수 있게 해 주는 유용한 방법이다. 이 연구에서는 CGH를 이용하여 두경부 편평상피세포암세포주 SNU-1041, SNU-1066, SNU-1076, PCI-1, PCI-13, PCI-50의 염색체 이상을 분석하였으며, 이를 바탕으로 두경부 편평상피세포암의 발생 및 진행과 관련된 염색체 이상을 밝히고자 하였다. 그 결과, 여섯 개의 세포주 모두에서 증가한 염색체 부위는 9q32-q34, 16q22-q24, 20q11.2-qter이었다. 염색체 3p10-p14의 감소가 다섯 개의 세포주에서 관찰되었으며, 3p15-p23, 4q22-q27, 4q31.3-qter, 18q21-q23의 감소가 네 개의 세포주에서 관찰되었다. 두경부 편평상피세포암 세포주의 염색체 이상에 대한 연구 결과는 암발생과 관련된 상세한 유전적 변화들을 이해하는 기본적인 단계가 될 것으로 판단된다. The knowledge about chromosomal aberrations manifestated in cancer has been spotlighted recently, and these studies are important for the development of cancer diagnosis and evaluation of prognosis. Purpose of this study was examine the chromosomal aberrations which were related in the development and progression of HNSCC. In this study, chromosomal aberrationsin HNSCC cell line, SNU-1041, SNU-1066, SNU-1076, PCI-1, PCI-13, and PCI-50 were analyzed using CGH. As a results, gains on 9q32-q34, 16q22-q24, and 20q11.2-qter were observed in all six cell lines. Loss on 3p10-p14 was analyzed in five cell lines. Also, losses on 3p15-p23, 4q22-q27, 4q31.3-qter, and 18q21-q23 were observed in four cell lines. These data about the patterns of chromosomal aberrations in HNSCC cell lines would be a basic step for understanding more detailed genetic events in the carcinogenesis.

비교유전체보합법에 의한 위암의 염색체이상 분석

박수연(Soo-Yeun Park),황혜진(Hye-Jin Hwang),이찬주(Chan-Joo Lee),박선화(Sun-Hwa Park) 대한해부학회 2004 Anatomy & Cell Biology Vol.37 No.6

종양의 염색체이상에 대한 세포유전학적 분석은 종양의 병인에 관련되는 다양한 유전자의 변형을 찾게 할 뿐 아니라, 질병의 진단과 예후를 판단하는데 중요한 부분이 되었다. 비교유전체보합법은 통상적인 세포유전학적 분석으로는 용이하지 않았던 모든 염색체 상에서의 상대적인 염색체 증감 변화를 한 번에 알 수 있게 해주며, degenerate oligonucleotide primedpolymerase chain reaction (DOP-PCR)에 의한 비교유전체보합법은 미세해부로 얻은 아주 적은 양의 종양 DNA를 대상으로도 염색체이상을 분석할 수 있는 빠르고 효과적인 방법이다. 이 연구에서는 한국인에게 호발하는 위암 조직을 대상으로 DOPPCR에 의한 비교유전체보합법을 시행하여 위암과 관련된 염색체이상을 밝히고자 하였다. 그 결과, 비전이성 위암에서 흔하게 증가되는 염색체 부위는 8q (64%), 4p12-q24 (64%), 5p13-q23 (64%), 13q21-q32 (64%), 6q11-q21 (55%), 7q (50%), 14q11.2-q21 (45%), 3q11-q13.3 (41%), 2q23-q32 (41%) 등이었다. 전이성 위암에서 흔하게 증가되는 염색체 부위는 8p21-qter (60%)였으며, 그 외에 5번 염색체 (54%), 20번 염색체 (42%), 6pter-q24 (51%), 1q21-qter (46%), 3p14-qter (46%), 22q (46%), 4번 염색체(43%)의 순서로 염색체 증가를 관찰할 수 있었다. 감소부위는 증가부위 보다 적게 관찰되었으며, 비전이성 위암에서는 1p34-pter (23%), 16q23-q24 (18%), 19q13 (18%)의 감소가 관찰되었다. 한편, 전이성 위암에서 가장 흔히 감소하는 염색체는 X 염색체(37%)와 1p33-pter (37%)이었고, 16p (23%)의 감소도 확인할 수 있었다. 이 연구에서 밝혀진 염색체이상 부위는 위암의 진행과 전이에 관여하는 유전자를 규명하고 분석하는 데 중요한 자료로 이용될 것이다. Chromosomal abnormalities, which are valuable markers for diagnosis and prognosis of cancer, provide useful clues in characterizing cancer at molecular level. Gastric cancer is the major cause of cancer deaths in Asian countries, including Korea. Genetic changes during the progression and metastasis of gastric cancer remain unclear. Recently, technique of degenerate oligonucleotide primed (DOP) PCR-comparative genomic hybridization (CGH) permits genetic imbalances screening of the entire genome using only small amounts of tumor DNA. In non-metastatic gastric cancers the common sites of copy number increases were detected at 8q (64%), 4p12-q24 (64%), 5p13-q23 (64%), 13q21-q32 (64%), 6q11-q21 (55%), 7q(50%), 14q11.2-q21 (45%), 3q11-q13.3 (41%), and 2q23-q32 (41%). In metastatic gastric cancers, the frequent sites of gains were detected at 8p21-qter (60%), 5 (54%), 20 (42%), 6pter-q24 (51%), 1q21-qter (46%), 3p14-qter (46%), 22q (46%), and 4 (43%). Deletion or chromosomal loss was found to be less frequent in this study. The frequent sites of copy number decreases were detected at 1p34-pter (23%), 16q23-q24 (18%), and 19q13 (18%) in non-metastatic gastric cancers. In metastatic gastric cancers, chromosome losses were detected at X (37%), 1p33-pter (37%), and 16p (23%). The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes respectively involved in the tumorigenesis of gastric cancer.

가임기 여성의 유방암 토모치료 시 난소선량 평가비교

이수형,박수연,최지민,박주영,김종석,Lee, Soo Hyeung,Park, Soo Yeun,Choi, Ji Min,Park, Ju Young,Kim, Jong Suk 대한방사선치료학회 2014 대한방사선치료학회지 Vol.26 No.2

목 적 : 최근 시행되고 있는 가임기 여성의 유방암 토모치료 시 치료영역 외에서 발생되는 산란 및 누설에 의한 난소산란선량을 측정하여 평가하고자 한다. 대상 및 방법 : 인체모형팬텀(Aldorson Rando phantom, USA)을 대상으로 전산화단층영상 2.5 mm 획득 후, Tomotherapy Planning station(Tomotherapy, Inc, USA)을 이용하여 좌측 유방암 환자의 토모테라피 치료계획(Tomotherapy Helical & Tomotherapy Direct)을 수립하였다. 난소의 산량 선량 측정을 위한 측정 지점은 치료계획면적의 30 cm 아래 떨어진 골반의 좌우 위치로 직경 1.5 mm, 길이가 12mm인 저에너지용 보상필터가 들어있는 종류의 유리선량계 (GD-352M, ASAHI TECHNO GLASS CO, Japan)를 이용하여 각 5회씩 측정하여 평균하였으며, 선형지수-선량반응모델을 이용한 장기등가선량(organ equivalent dose: OED)으로 평가하였다. 결 과 : 토모 Helical 및 토모 Direct의 두 가지 방식으로 측정된 난소의 산란선량은 좌측 난소부위가 각각 평균 $64.94{\pm}0.84mGy$, $37.64{\pm}1.20mGy$이고, 우측 난소부위가 평균 $64.38{\pm}1.85mGy$, $32.96{\pm}1.11mGy$로 나타났다. 이는 토모치료 시 비교적 모니터 단위(MU)가 크고 조사 시간이 긴 토모Helical 방식이 토모Direct에 비하여 측정된 산란선량의 양이 보다 약 1.8배 높은 경향을 보였다. 결 론 : 가임기 여성의 유방암 토모테라피 시 발생하는 좌우측 난소의 산량선량은 ICRP 권고 선량이하로, 불임 및 2차 암 발생에 대한 우려 수준은 현저히 낮지만 향후 유방암 발생 연령층이 낮아지고, 토모테라피와 같이 고정밀 영상유도장치를 이용한 방사선치료가 발달할수록, 가임기 여성 환자의 난소산란선량에 대한 임상적 추적조사가 더욱 필요할 것으로 사료된다. Purpose : The aim of this study is to evaluate unwanted scattered dose to ovary by scattering and leakage generated from treatment fields of Tomotherapy for childbearing woman with breast cancer. Materials and Methods : The radiation treatments plans for left breast cancer were established using Tomotherapy planning system (Tomotherapy, Inc, USA). They were generated by using helical and direct Tomotherapy methods for comparison. The CT images for the planning were scanned with 2.5 mm slice thickness using anthropomorphic phantom (Alderson-Rando phantom, The Phantom Laboratory, USA). The measurement points for the ovary dose were determined at the points laterally 30 cm apart from mid-point of treatment field of the pelvis. The measurements were repeated five times and averaged using glass dosimeters (1.5 mm diameter and 12 mm of length) equipped with low-energy correction filter. The measures dose values were also converted to Organ Equivalent Dose (OED) by the linear exponential dose-response model. Results : Scattered doses of ovary which were measured based on two methods of Tomo helical and Tomo direct showed average of $64.94{\pm}0.84mGy$ and $37.64{\pm}1.20mGy$ in left ovary part and average of $64.38{\pm}1.85mGy$ and $32.96{\pm}1.11mGy$ in right ovary part. This showed when executing Tomotherapy, measured scattered dose of Tomo Helical method which has relatively greater monitor units (MUs) and longer irradiation time are approximately 1.8 times higher than Tomo direct method. Conclusion : Scattered dose of left and right ovary of childbearing women is lower than ICRP recommended does which is not seriously worried level against the infertility and secondary cancer occurrence. However, as breast cancer occurrence ages become younger in the future and radiation therapy using high-precision image guidance equipment like Tomotherapy is developed, clinical follow-up studies about the ovary dose of childbearing women patients would be more required.

두경부 편평상피세포암 세포주의 염색체 이상 분석:비교유전체보합법과 Array 비교유전체보합법

신유리(You Ree Shin),박수연(Soo-Yeun Park),이동욱(Dong Wook Lee),김한수(Han Su Kim),고영민(Young Min Go),박현주(Hyun Joo Park),정성민(Sung Min Choung) 대한두경부종양학회 2008 대한두경부 종양학회지 Vol.24 No.1

Head and neck squamous cell carcinoma(HNSCC) is notorious for its poor outcome and increasing incidence. But, the studies of cytogenetic analysis in HNSCC are relatively rare, because of difficulties in culturing solid tumor cells and complexity in chromosomal DNA abberations associated with the lesions. The purpose of this study is to evaluate the location of chromosomal aberrations in Korean HNSCC cell lines (SNU-1041, 1066, and 1076) with comparative genomic hybridization(CGH) and array based CGH(array-CGH). Chromosomal gains of 3q23-q27, 5p13-p15.3, 7p21-pter, 8q11.2-q12, 8q21.1-qter, 9q22-q34, 16q22-q24, and 20q11.2-qter, as well as chromosomal losses on 3p10-p14 were found in all 3 SNU cell lines. Losses on 3p15-p23, 4q22-q27, 4q31.3-qter, 6q14-q15, 7q31-q34, 8p12-pter, 18q21-q23, and 21q11.2-q12 were observed in 2 of 3 cell lines. In array-CGH, many genes were altered including gains of PIK3CA, MYC, EVI1, MAD1L1 genes and losses of SERPIN genes. These aberrations of gene and chromosome coincide with other results of study, generally. These data about the patterns of chromosomal aberrations could be a basic step for understanding more de-tailed genetic events in the carcinogenesis and also provide information for diagosis and treatment in HNSCC.

한국인 후두 편평 상피 세포암의 유전체 이상분석:Array 비교 유전체 보합법

조윤희(Yoon Hee Cho),박수연(Soo-Yeun Park),이동욱(Dong Wook Lee),김한수(Han Su Kim),이자현(Ja Hyun Lee),박혜상(Hae Sang Par),정성민(Sung Min Chung) 대한두경부종양학회 2008 대한두경부 종양학회지 Vol.24 No.2

Head and neck squamous cell carcinoma(HNSCC) still has poor outcome, and laryngeal cancer is the most frequent subtype of HNSCC. Therefore, there is a need to develop novel treatments to improve the outcome of patients with HNSCC. It is critical to gain further understanding on the molecular and chromosomal alteration of HNSCC to identify novel therapeutic targets but genetic etiology of squamous cell carcinoma of the larynx is so complex that target genes have not yet been clearly identified. Array based CGH(array-CGH) allows investigation of general changes in target oncogenes and tumor suppressor genes, which should, in turn, lead to a better understanding of the cancer process. In this study, We used genomic wide array-CGH in tissue spe-cimens to map genomic alterations found in laryngeal squamous cell carcinomas. As results, gains of MAP2, EPHA3, EVI1, LOC389174, NAALADL2, USP47, CTDP1, MASP1, AHRR, and KCNQ5, with losses of SRRM1L, ANKRD19, FLJ39303, ZNF141, DSCAM, GPR27, PROK2, ARPP-21, and B3GAT1 were observed frequently in laryngeal squamous cell carcinoma tissue specimens. These data about the patterns of genomic alterations could be a basic step for understanding more detailed genetic events in the carcinogenesis and also provide information for diagnosis and treatment in laryngeal squamous cell carcinoma. The high resolution of array-CGH combined with human genome database would give a chance to find out possible target genes which were gained or lost clones.