http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
소 모색관련 MC1R 유전자의 SNP와 관련한 MGB probe에 기초한 real-time PCR을 이용한 한우육과 Holstein육의 판별
박성도,김태중,이재일,Park, Sung-Do,Kim, Tae-Jung,Lee, Jae-Il 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.1
The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo meat, we performed a single nucleotide polymorphism (SNP) analysis in Melanocortin 1 receptor (MC1R) gene using TaqMan$^{(R)}$ MGB probe-based real-time PCR. Two specific probes (one for Hanwoo and the other for Holstein and Black angus) were designed. At the 5' end of 2 TaqMan$^{(R)}$ MGB probes, 6-carboxyfluorescein (FAM) was labeled for Hanwoo, and VIC for Holstein and Black angus. As a result, Hanwoo samples showed FAM-positive signal only, whereas other samples showed VIC-positive. This result suggests that the TaqMan$^{(R)}$ MGB probe based real-time PCR technique would be very accurate, easy and reproducible method to discriminate between Hanwoo meat and Holstein/Black angus meat.
소고기의 유통 단계별 병원성 미생물 오염도에 관한 연구
박성도 ( Seong Do Park ),김용환 ( Yong Hwan Kim ),고바라다 ( Ba Ra Da Koh ),김철희 ( Cheol Hee Kim ),윤병철 ( Byeong Cheol Yoon ),김조균 ( Cho Kyun Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2
Contamination levels of pathogenic microorganisms in 145 cases of beef, which were distributed in Gwangju province, had been investigated in each distributed stage and also monitored by general bacterial count and E coli count index. General bacterial count of beef from the slaughterhouse was 10(4) cfu/g less than the level of promotion(10(5) cfu/cm2) and E coli count index was also under the level of 10(2) cfu/cm2 recommended level of the ministry of agriculture and forestry. Pathogenic microorganisms were detected from 23.2% of samples in the consumption stage, 12.5% in the slaughtering stage and 5.6% in the transporting and processing stage. Staphylococcus aureus was isolated in the largest number and its ratio was 9.0%, listeria monocytogenes 5.5% and salmonella spp 1.4%. There were no samples that bacteria had been detected dually. E coli O157:H7 and campylobacter jejuni were not isolated. In raw and chilled beef, isolation rate of pathogenic microorganisms were 13.3% and 16.5% each. Especially in raw beef, L monocytogenes was isolated in 3 samples among 30 cases (10%) and S aureus in one sample (3.3%). According to a scale of meat store, isolation rates of pathogenic microorganisms were different. It was 28.6% in the small-scale meat store and 16.7% in the large-scale meat store each. Four cases (16.7%) of S aureus were isolated in the large-scale meat store and seven cases (20.0%) of Lmonocytogenes and 2 cases (5.7%) of salmonella spp were isolated in the small-scale meat store. S aureus was isolated in two places among 10 feeding facilities of the elementary school. This result shows that the sanitation of elementary school feeding facilities is so poor and more careful policy consideration is needed. Eleven strains of S aureus isolated showed β-hemolysis on blood agar, 1 strain α-hemolysis, and 1 strain γ-hemolysis. Isolated strains of L monocytogenes were reconfirmed in 560 bp. by PCR. Conclusively, these results show that the sanitary condition in the stages of slaughtering, transportation-processing and consumption influences the degree of pathogenic microorganisms contamination in beef severely. It is necessary to apply thoroughly hazard analysis critical control point in a process of beef distribution and also to develop rapid test methods for microorganism diagnosis. This effort is very important for the supply of safe and clean meat from farm to table and helpful for the improvement of public health.
바바리양에서 발생한 Streptococcus equi subsp. zooepidemicus 감염증
고바라다,박성도,김재익,박종태,Koh, Ba-Ra-Da,Park, Seong-Do,Kim, Jae-Ik,Park, Jong-Tae 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.4
An eight years old female barbary sheep (Ammotragus lervia), which bred at the Gwangju Uchi Park Zoo had shown anorexia, depression, respiratory problem for several weeks after parturition. In necropsy, extensive necrotizing pneumonia was found with severe immunocytes infiltration in the alveolar spaces and bronchioles. Pulmonary pleura were thickened with fibrin and inflammatory cells. Bacteria were isolated from lung and identified as Streptococcus equi subsp. zooepidemicus (SEZ) by biochemical tests and PCR on sodA and gusA genes, though seel gene was not detected. Isolation of zoonotic SEZ in public place such as a zoo should be emphasized for the public health mangagement.
고바라다 ( Ba Ra Da Koh ),김현중 ( Hyun Joong Kim ),박덕웅 ( Duk Woong Park ),박성도 ( Seong Do Park ),김재익 ( Jae Ik Kim ),박종태 ( Jong Tae Park ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.3
Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhans-type multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acid-fast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism. The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. These cases are interesting and important in public health aspect that M bovis-infected cattle were found during a routine post-mortem inspection at slaughter house.
나호명 ( Ho Myung Na ),배성열 ( Seong Yeol Bae ),고바라다 ( Ba Ra Da Koh ),박재성 ( Jae Sung Park ),서윤정 ( Yun Jeong Seo ),정하진 ( Ha Jin Jeong ),박자윤 ( Ja Yoon Park ),박성도 ( Seong Do Park ),김은선 ( Eun Sun Kim ),김용환 ( Y 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.2
The antibody titers against Coxiella burnetii (Q fever) among cattle raised in Gwangju area were analyzed from February to October in 2015. The prevalence of antibodies in collected bulk-tank milk from 7 dairy cattle farms was 57.1% and the seroprevalence of 210 dairy cows randomly selected from those farms was 7.1%. By age, the seroprevalence was 3.3% in less than 4 years of age, 9.0% between 4 and 7 years of age, and 28.6% in more than 8 years of age. On the other hand, the seroprevalence of the Hanwoo cattle was 0.4%. The result suggested that the antibodies against Coxiella burnetii increase as the age of the dairy cows increases and therefore, it is necessary to keep monitoring the prevalence of Q fever in Gwangju area.