한국 재래계의 텔로미어 길이의 유전력과 유전양상 분석

박단비,조은정,손시환 한국가금학회 2013 한국가금학회 정기총회 및 학술발표회 Vol.2013 No.11

닭 텔로미어 길이의 유전력 추정과 유전 전이 양상

박단비,손시환,Park, Dan Bi,Sohn, Sea Hwan 한국가금학회 2014 韓國家禽學會誌 Vol.41 No.3

텔로미어는 진핵 세포의 염색체 말단으로, 직렬 반복 DNA 염기 서열과 shelterin 단백질 복합체로 구성되어 있다. 텔로미어의 기능은 염색체를 보호하는 것으로 체세포의 텔로미어 길이는 세포 분열시 DNA 복제 결실로 인해 연령이 증가함에 따라 감소하는 경향이 있다. 그러나 유전적, 후생유전학적 및 환경적 수준에서 여러 가지 요인이 텔로미어 길이에 영향을 미친다. 따라서 본 연구에서는 닭의 텔로미어 길이의 유전력을 추정하고, 이들의 유전전이 양상을 살펴보고자 하였다. 텔로미어 길이는 백혈구를 이용하여 양적 형광접합보인법(Q-FISH)과 양적 중합효소 연쇄반응법(qRT-PCR)으로 분석하였다. 분석 결과, 텔로미어 길이의 유전력은 자손과 부모 회귀 분석에 의해 출생 시 0.9로 추정되었고, 10 주령 및 30주령 때 부 분산 분석에 의해 0.03과 0.04로 추정되었다. 부와 자손 간(r=0.348) 및 모와 자손 간(r=0.380) 텔로미어 길이는 모두 유의한 정의 상관 관계를 보였다. 따라서 닭 텔로미어의 유전 전이 양상은 부모 양쪽 모두로부터 비슷하게 자식에 영향을 미치는 것으로 판단된다. 더불어 암수 자손에 미치는 영향 또한 유사한 것으로 나타났다. 이러한 결과는 부모의 텔로미어 길이의 각인이 성염색체의 유전자가 아닌 상염색체의 유전자에 의해 조절되는 것을 의미한다. 또한, 산모 연령에 따른 출생 자손의 텔로미어 길이는 차이가 없는 것으로 나타났다. 따라서 모체의 연령이 출생 자손의 텔로미어 길이에 영향을 미치지 않는데, 이는 수정란의 초기 배아 단계에서 세포적 reprogramming이 이루어지기 때문으로 사료된다. Telomeres are the ends of the eukaryotic chromosomes and consist of a tandem repetitive DNA sequence and shelterin protein complex. The function of telomere is to protect chromosome. Telomere length in somatic cells tends to decrease with organismal age due to the end replication problem. However, several factors at the genetic, epigenetic and environmental level affect telomere length. In this study, we estimated heritability of telomere length and investigated inheritance of telomeres in a chicken. Telomere length of lymphocytes was analyzed by semi-quantitative polymerase chain reaction using telomere primer and quantitative fluorescence in situ hybridization using telomeric DNA probe. In results, heritability of telomere length was estimated 0.9 at birth by offspring-parent regression analysis and was estimated 0.03 and 0.04 at 10 and 30 weeks old, respectively, by parental variance analysis. There was a significant positive correlation in telomere length between father and their offspring (r=0.348), and mother and their offspring (r=0.380). In inheritance patterns of telomere length, the influence of paternal and maternal effect on their offspring was similar. The influence of inherited telomeres on male and female progeny was also roughly alike. These results implicated that imprinting of parental telomere length was regulated by autosomal genes, not sex linked genes. In addition, telomere length of offspring at birth did not differ along with their maternal age. Thus, maternal age does not affects telomere length in their offspring at birth owing to cellular reprogramming at early embryonic stage.

한국인 영어 학습자의 등급화된 즉흥 발화 OPic 음성자료의 초분절적 특징 연구

박단비,이석재 국제언어인문학회 2019 인문언어 Vol.21 No.2

In this study, we analyzed 60 Korean learners’ suprasegmental features from the OPIc speech data administered by the ACTFL committee. According to the evaluation method prescribed by the ACTFL committee, the data are classified into six grades from the lowest IL to the highest AL, and it is meaningful that they can examine the spontaneous speech of Korean learners. Pauses, speech rate and F0 range were analyzed using the Praat speech analysis program. Pause duration was measured by detecting the silent period over 250ms. The speech rate was calculated by dividing the time spent on speaking sentence by the syllable per sentence. The F0 range was measured as the pitch curve of the sentence. The result of the study can be derived as follow: Factors that differ among the six groups of different grades and that appear to have high influence on grading are the inter-sentential pause duration, intra-sentential pause duration, and speech rate. In other words, the higher the grade, the shorter the duration of the pauses and the faster the speech rate. There were no statistically significant differences between the grades in the intra-sentential pause frequency and the F0 range.

육계에 비타민 C 및 E의 첨가 급여가 telomere 함량 및 스트레스 연관 DNA 유전자 발현에 미치는 영향

박단비,조은정,장인석,문양수,손시환 한국가금학회 2012 한국가금학회 정기총회 및 학술발표회 Vol.29 No.-

Can ICT Development of Partner Countries Matter for Trade?

박단비,이미혜 한국EU학회 2023 EU학연구 Vol.28 No.1

This paper examines the role of ICT development on Korea’s export and import performance from 2013 to 2018 with its trading partner countries including some European countries such as France, Germany, Italy, the Netherlands and Switzerland. We investigate whether the relative development in ICT which is measured as the differences in ICT levels between Korea and its trading partner can stimulate trade between countries and whether the relative development of ICT can play a significant role to explain Korea’s trade with other countries. Our empirical analysis shows that development of the trade between two countries tend to grow faster as both countries participating in trade exhibit similar level of ICT. We also find that the development of ICT itself or the ICT development of a trading partner country does not appear to be significant in promoting trade between countries implying mutual development of ICT is necessary to increase trade between countries. Furthermore, it can mitigate the negative impact of geographical distance as the relative development of ICT becomes small. Our empirical analysis suggests that ICT development could enhance trade performance between countries similar to the findings in the existing literature and it emphasizes the relative development of ICT between trading countries matters to make ICT development contribute to boost international trade.

텍스트와 이미지에 나타난 메르스 감염병 프레임 분석 : 인스타그램( Instagram) 내용분석을 중심으로

박단비,권상희,강보영,김세진 한국소통학회 2018 한국소통학보 Vol.17 No.3

본 연구는 인스타그램(Instagram)에 게시된 메르스(MERS) 관련 텍스트와 이미지를 대상으로 프레임 분석을 실시했다. 분석기간은 2015년 5월 20일부터 7월 28일까지 이다. 주요 분석 결과는 다음과 같다. 1시기의 경우 메르스에 대한 조심 예방 등 메르스에 대한 불안 프레임이 작용하였다. 이 시기의 이미지 프레임 또한 언어 프레임과 비슷한 양상으로 새로운 감염 병에 대한 공포프레임이 형성되었다. 한편 2시기에 경우 메르스 주변으로 일상생활에 표현에 대한 형성이 1시기보다 많이 형성된 것을 발견할 수 있었다. 2시기의 이미지 프레임의 경우 일상에 비치는 정체성 프레임이 형성되었다. 제3시기의 경우에는 감기 시작 예방 등이 나타나고 있고, 이미지 프레임 또한 극복을 이미지를 프레임 형성하는 것을 알아 볼 수 있었다. 위의 결과로 알아 볼 수 있듯이 이미지프레임과 언어프레임은 비슷한 양상을 보이며 프레임 형성을 한다고 발견할 수 있었다. 본 연구를 통해 인스타그램 사용자들이 메르스 이슈에 대해 어떤 이미지 프레임을 형성하고 어떤 텍스트 프레임을 형성하였는지를 분석했다는 것이 본 연구의 의의라 할 수 있다. This paper explores the image analysis and Text Semantic Network Analysis through texts and images for MERS virus, the recent main issue of our society, in Instagram. Through the study will examine what image frame Instagram forms and what language frame Instagram uses about MERS. For this, frame theory, image frame theory, images of historical disaster, and language semantic network on Instagram were carefully considered. To analyze images related to MERS issue in Instagram, 1,004 images were collected through stratification sampling of 10,000 images that can be searched by the image search platform. The image plot software was used for method of analysis. This study collected posts with hash tag ‘#MERS’ from May 20, 2015 to July 28 in order to analyze Instagram posts on MERS issue. A result of analyzing images and posts about MERS in Instagram is as follows. For the period 1, anxiety frame for MERS such as prevention for MERS was acted. Image frame for the period 1 is also in a similar pattern of language frame, fear frame was formed for a new infection disease. On the other hand, for the period 2, it could be found that expressions about MERS in everyday life were formed more than in the period 1. In case of an image frame in the period 2, identity frame from daily life was formed. For the period 3, prevention of signs of an oncoming flu was showed, and it could be found that overcoming image frame was formed in case of an image frame. As you can see the above result, an image frame and language frame forms frame showing in a similar pattern. Through this study, analyzing what image frame Instagram users formed and what meaning of posts they wrote about MERS issue can be regarded as significant in this study.

다문화·한부모여성의 사회자본이 생활만족도에 미치는 영향

박단비 한국가족자원경영학회 2016 한국가족자원경영학회 학술대회논문집 Vol.2016 No.11

Financial Constraint and Corporate Employment: Evidence from the Emerging Economy

박단비 한국국제금융학회 2016 국제금융연구 Vol.6 No.1

This paper investigates the impact of financial constraint on corporate employment decision during the financial crisis using Korean firm-level data. During the financial crisis, credit constrained firms need to consider reducing employees to cut down the expenses and rescue from financial distress. We find that firm-specific determinants have significant effects on the relation between credit constraint and corporate employment. The empirical results show that in case of the SMEs, high loan ratio cause high probability to reduce employees. However, loan ratio does not have a significant effect on employment decision in case of large firms. We can induce that financial burden on SMEs lead to high unemployment rate in Korea. This study will provide policy implication that stimulating micro-finance on SMEs is also important to improve employment situation.

Fabrication and Characterization of Graphene Oxide-Coated Plate for Efficient Culture of Stem Cells

박단비,박재범,이지훈,Shim Chang Jae,김민성,이태용,임정옥 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

Background: For stem cell applications in regenerative medicine, it is very important to produce high-quality stem cells in large quantities in a short time period. Recently, many studies have shown big potential of graphene oxide as a biocompatible substance to enhance cell growth. We investigated if graphene oxide-coated culture plate can promote production efficiency of stem cells. Methods: Three types of graphene oxide were used for this study. They are highly concentrated graphene oxide solution, single-layer graphene oxide solution, and ultra-highly concentrated single-layer graphene oxide solution with different single-layer ratios, and coated on cell culture plates using a spray coating method. Physiochemical and biological properties of graphene oxide-coated surface were analyzed by atomic force microscope (AFM), scanning electron microscope (SEM), cell counting kit, a live/dead assay kit, and confocal imaging. Results: Graphene oxide was evenly coated on cell culture plates with a roughness of 6.4 ~ 38.2 nm, as measured by SEM and AFM. Young’s Modulus value was up to 115.1 GPa, confirming that graphene oxide was strongly glued to the surface. The ex vivo stem cell expansion efficiency was enhanced as bone marrow-derived stem cell doubling time on the graphene oxide decreased compared to the control (no graphene oxide coating), from 64 to 58 h, and the growth rate increased up to 145%. We also observed faster attachment and higher affinity of stem cells to the graphene oxide compared to control by confocal microscope. Conclusion: This study demonstrated that graphene oxide dramatically enhanced the ex vivo expansion efficiency of stem cells. Spray coating enabled an ultra-thin coating of graphene oxide on cell culture plates. The results supported that utilization of graphene oxide on culture plates can be a promising mean for mass production of stem cells for commercial applications. Background: For stem cell applications in regenerative medicine, it is very important to produce high-quality stem cells in large quantities in a short time period. Recently, many studies have shown big potential of graphene oxide as a biocompatible substance to enhance cell growth. We investigated if graphene oxide-coated culture plate can promote production efficiency of stem cells. Methods: Three types of graphene oxide were used for this study. They are highly concentrated graphene oxide solution, single-layer graphene oxide solution, and ultra-highly concentrated single-layer graphene oxide solution with different single-layer ratios, and coated on cell culture plates using a spray coating method. Physiochemical and biological properties of graphene oxide-coated surface were analyzed by atomic force microscope (AFM), scanning electron microscope (SEM), cell counting kit, a live/dead assay kit, and confocal imaging. Results: Graphene oxide was evenly coated on cell culture plates with a roughness of 6.4 ~ 38.2 nm, as measured by SEM and AFM. Young’s Modulus value was up to 115.1 GPa, confirming that graphene oxide was strongly glued to the surface. The ex vivo stem cell expansion efficiency was enhanced as bone marrow-derived stem cell doubling time on the graphene oxide decreased compared to the control (no graphene oxide coating), from 64 to 58 h, and the growth rate increased up to 145%. We also observed faster attachment and higher affinity of stem cells to the graphene oxide compared to control by confocal microscope. Conclusion: This study demonstrated that graphene oxide dramatically enhanced the ex vivo expansion efficiency of stem cells. Spray coating enabled an ultra-thin coating of graphene oxide on cell culture plates. The results supported that utilization of graphene oxide on culture plates can be a promising mean for mass production of stem cells for commercial applications.

요양보호사의 감성지능이 혁신행동에 미치는 영향: 임파워먼트와 지식공유의 매개효과를 중심으로

박단비 한국산학기술학회 2022 한국산학기술학회논문지 Vol.23 No.12

The purpose of this study was to verify the mediating effect of empowerment and knowledge sharing in the relationship between the emotional intelligence and innovative behavior of a caregiver. For this study, a survey was conducted covering 348 nursing care workers for about 3 weeks from May 3rd to 24th, 2022. The data were analyzed using the AMOS 24.0 and SPSS 24.0 statistical programs. The results of the analyses were as follows; First, the emotional intelligence of the caregivers had a significant and positive impact on their innovative behavior. Second, the emotional intelligence of the caregivers had a significant and positive impact on empowerment. Third, the emotional intelligence of the caregivers had a significant and positive impact on knowledge sharing. Fourth, the empowerment of the caregivers had a significant and positive impact on innovative behavior. Fifth, the sharing of knowledge among the caregivers had a significant and positive impact on their innovative behavior. Sixth, in the relationship between the caregivers' emotional intelligence and innovative behavior, empowerment and knowledge sharing had a significant mediating effect. The new implication of this study in the field of human resources (HR) management and training in long-term care facilities is that it empirically verified the possibility of enhancing the emotional intelligence, empowerment, and level of knowledge sharing of caregivers while encouraging innovative behavior. 본 연구의 목적은 요양보호사의 감성지능과 혁신행동의 관계에서 임파워먼트와 지식공유의 매개효과를 검증하는것이다. 본 연구를 위해 2022년 5월 3일부터 24일까지 약 3주 동안에 요양보호사 348명을 대상으로 설문조사를 실시하였고, AMOS 24.0과 SPSS 24.0 통계프로그램을 사용하여 자료를 분석하였다. 분석결과는 다음과 같다. 요양보호사의감성지능은 혁신행동에 유의한 정의 영향을 미치는 것으로 나타났다. 둘째, 요양보호사의 감성지능은 임파워먼트에 유의한 정의 영향을 미치는 것으로 나타났다. 요양보호사의 감성지능은 지식공유에 유의한 정의 영향을 미치는 것으로 나타났다. 요양보호사의 임파워먼트는 혁신행동에 유의한 정의 영향을 미치는 것으로 나타났다. 요양보호사의 지식공유는혁신행동에 유의한 정의 영향을 미치는 것으로 나타났다. 마지막으로, 요양보호사의 감성지능과 혁신행동의 관계에서임파워먼트와 지식공유는 유의한 매개효과가 있는 것으로 나타났다. 본 연구는 요양보호사의 감성지능, 임파워먼트, 지식공유 수준을 향상시키면서 혁신행동을 이끌어 낼 수 있다는 점을 실증적으로 확인함으로써 요양기관의 인사관리 및교육 분야에 새로운 시사점을 제공하였다.