연부조직의 기생충 감염의 세포학적 소견

박경미,고일향,Park, Kyeong-Mee,Ko, Ill-Hyang 대한세포병리학회 1995 대한세포병리학회지 Vol.6 No.1

In the past, parasitic diseases were a major problem in public health in Korea. In recent years, however, nematodiasis that used be prevalent are no longer a serious problem. Instead some cestodiasis, particularly cysticercosis and sparganosis have become comparatively more important in recent years. Parasitic infestation of soft tissue is presented as a subcutaneous nodule or mass with nonspecific clinical manifestations. We experienced 4 cases of parasitic infestation in the soft tissue diagnosed by fine needle aspiration (FNA). Three out of four cases were histologically confirmed, two out of four cases were cysticercosis and one case was confirmed as sparganosis. FNA may be useful in providing a diagnosis in cases of parasitic infestation.

갑상선 질환의 진단에 있어서 세침흡인세포학적 검사의 중요성 - 조직학적으로 확진된 153예에 대한 연구 -

박경미,고일향,Park, Kyeong-Mee,Ko, Ill-Hyang 대한세포병리학회 1996 대한세포병리학회지 Vol.7 No.2

This is a retrospective review of fine-needle aspiration cytology(FNAC) smears of 153 cases of thyroid disease performed during August 1989 to July 1995, which were confirmed histologically following surgical operations. FNAC results showed 63 cases(41.2%) of adenomatous goiter, 45 cases(29.4%) of papillary carcinoma, 29 cases(19.0%) of follicular neoplasm, 4 cases(2.6%) of follicular variant of papillary carcinoma, 4 cases(2.6%) of Hashimoto's thyroiditis, 4 cases(2.6%) of $H\ddot{u}rthle$ cell neoplasm, 2 cases(1.3%) of medullary carcinoma and one case(0.7%) each of subacute thyroiditis and of anaplastic carcinoma. The overall accuracy of cytological diagnosis was 83.7%. These data strongly suggest thyroid FNAC is a reliable preoperative diagnostic tool, but FNAC has been less valuable in the diagnosis of follicular lesions than any other disease of the thyroid. Adenomatous goiter was not infrequently interpreted as follicular neoplasia that requires surgery for diagnostic conformation and vice versa. The following findings are considered to be compatible with follicular neoplasm: 1) microfollicles, 2) nuclear grooving, 3) irregularity of nuclear membrane, and 4) irregular arrangement or crowding of follicular cells in groups. The FNAC criteria of adenomatous goiter are as follows: 1) atrophic follicular cells, 2) presence of macrophages, 3) abundant colloid, and 4) large follicles. It is recommended that aspiration of thyroid lesions in order to analyse with critical clinico-pathological approach and surgery is considered only for nodules that are clinically suspicious or unresponsive to hormone therapy or when a diagnosis of follicular neoplasm is made.

유방암종과 양성 유방 질환의 세침흡인 검체와 조직에서의 카뎁신 D 단백질의 발현

박경미,고일향,Park, Kyeong-Mee,Ko, Ill-Hyang 대한세포병리학회 2000 대한세포병리학회지 Vol.11 No.2

Cathepsin D is a protease which is known to facilitate invasion and metastasis of breast carcinoma. Overexpression of cathepsin D is associated with poor clinical outcome and biologic aggressiveness of the breast cancer. We underwent immunocytochemical assay(ICA) for cathepsin D in fine needle aspiration cytology(FNAC) specimens from the breast carcinoma and benign breast diseases. In FNAC specimens cathepsin D was expressed in 21(42.9%) out of 49 cases of invasive ductal carcinoma, whereas negative result was observed in all 15 cases of benign breast diseases including 7 fibroadenomas, 6 fibrocystic diseases, and 2 benign ductal hyperplasias. Among the 11 FNAC specimens from ductal carcinoma in situ(DCIS), cathepsin D was expressed in 3 cases(27.3%). In FNAC specimens immunocytochemistry for cathepsin D showed positive result in 24 out of 60 carcinomas(sensitivity, 40%) and negative result in 15 out of all 15 benign breast diseases(specificity, 100%). No significant correlation was noted between cathepsin D expression in FNAC specimen and clinicohistological characteristics of the breast carcinoma, such as hormone receptors and cell differentiation. In conclusion, ICA of cathepsin D in FNAC specimens thought to be a good adjunct to differentiate malignancy from benign breast diseases.

모기질 세포종 - 증례 보고 -

박경미,심정원,고일향,Park, Kyeong-Mee,Shim, Jung-Weon,Ko, Ill-Hyang 대한세포병리학회 1991 대한세포병리학회지 Vol.2 No.2

Pilomatricoma is a well defind and characteristic entity histologically, but this tumor may be encountered during aspiration biopsy of subcutaneous mass, and is, occasionally, confused with other neoplasms, particularly squamous cell carcinoma, basal cell carcinoma, and epidermal inclusion cyst. Recently, we have experienced a case of pilomatricoma diagnosed by aspiration biosy. The smear of the aspirate revealed anucleated "ghost" squamous cells, basaloid or basophilic squamous cells and nucleated squamous cells, which lack nuclear features of malignacy. These findings are consistent with pilomatricoma.

유방 세침흡인 세포검사의 진단적 함정: 위양성과 위음성

박경미,Park, Kyeong-Mee 대한세포병리학회 2007 대한세포병리학회지 Vol.18 No.2

Fine needle aspiration cytology (FNAC) has become a highly preferred, minimally invasive diagnostic tool of choice in the diagnosis of a palpable breast mass owing to its sensitivity, specificity, cost-effectiveness, and expediency. Although breast needle biopsies have been widely employed recently due to the increased detection rate of non-palpable early lesions, the importance of the use of FNAC cannot be underestimated. It comprises part of the diagnostic triad for the breast along with a physical examination and mammography, which together contribute to an increasing diagnostic accuracy. The differential diagnosis of a benign and malignant lesion is of the utmost importance in the diagnosis of breast lesions, and therefore the understanding of the possible diagnostic pitfalls is of great importance.

귀밑샘에 발생한 림프상피암종의 세침흡인 세포학적 소견 - 1 예 보고 -

임성직,김정연,박경미,Lim, Sung-Jig,Kim, Jung-Yeon,Park, Kyeong-Mee 대한세포병리학회 2002 대한세포병리학회지 Vol.13 No.2

Lymphoepithelial carcinoma, also known as malignant lymphoepithelial lesion or lymphoepithelioma, is a rare tumor in salivary glands. Lymphoepithelial carcinoma has a characteristic histological findings comprising irregularly-shaped nests of malignant epithelial cells within a lymphocyte-rich stroma, occasionally forming lymphoid follicles. We recently experienced a case of fine needle aspiration cytology (FNAC) of lymphoepithelial carcinoma of parotid gland in a 61-year-old male. The FNAC yielded a hypercellular smear of many irregular clusters of malignant epithelial cells in the background of lymphoid stroma.

암종으로 오인된 전이 폐포횡문근육종의 세침흡인 세포소견 -1예 보고-

김현정,임성직,박경미,Kim, Hyun-Jung,Lim, Sung-Jik,Park, Kyeong-Mee 대한세포병리학회 2005 대한세포병리학회지 Vol.16 No.1

Fine-needle aspiration cytology (FNAC) has been used extensively in the diagnosis of metastatic malignancies. However, metastatic soft tissue sarcomas are often overlooked, primarily due to the low frequency with/ which they occur. Here, we report a rare case of metastatic rhabdomyosarcoma in both cervical lymph nodes, which was detected by FNAC. A 45-year-old woman presented with anosmia, postnasal drip, and sneezing, symptoms which had persisted for 1 month. The patient was found to have a tumorous lesion at the upper portion of the mid-turbinate, with multiple enlarged cervical lymph nodes, and this lesion was examined closely at our facility. FNA cytology smears obtained from both cervical lymph nodes revealed a high degree of cellularity, and displayed cohesive clusters with gland-like spaces, as well as single isolated cells with abundant karyorrhectic debris. The tumor cells exhibited round to oval nuclei containing fine chromatin, occasional small nucleoli, and scanty cytoplasm, or a total lack of cytoplasm. Some of the tumor cells were arranged in multinucleated forms and abundant dense eosinophilic cytoplasms, reminiscent of a rhabdomyoblast. The histological findings on the lymph nodes revealed an outstanding sinusoidal infiltration and a prominent alveolar growth pattern, interspersed with occasional typical rhabdomyoblasts. The immunohistochemical results [desmin (+), myoglobin (+), myogenin (+), pan CK (-), synaptophysin (-), neuron specific enolase (-)] supported a confirmative diagnosis of alveolar rhabdomyosarcoma. Alveolar rhabdomyosarcoma is a representative sarcoma, which typically manifests with nodal metastasis and carcinoma-like clustering. The cytopathologist should remain alert upon encountering unusual morphology, so that the possibility of this condition, although somewhat remote, should not be dismissed or overlooked.

Panax ginseng C. A . Meyer ( 고려홍삼 ) 의 비사포닌의 분획의 항혈소판작용

박화진,이만휘,박경미,남기열,박기현 ( Hwa Jin Park,Man Hee Rhee,Kyeong Mee Park,Ki Yeul Nam,Ki Hyun Park ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.8

The non-saponin fraction(NSF: lipophilic fraction) from Korean red ginseng showed an inhibitory activity in the aggregation of human platelets induced by a high dose of thrombin (0.2 to 2 u/㎖), the extent of which was stronger than that of verapamil, a Ca^(2+) antagonist. NSF did not affect the elevation of the intracellular cAMP concentration which inhibits platelet aggregation, as shown in the case of verapamil, but inhibited the production of TXA₂ and the release of serotonin. NSF does not inhibit the release of arachidonic acid from membrane phospholipids, but inhibits a certain pathway transforming arachidonic acid to TXA₂. NSF may play a critical role in preventing and curing thrombotic diseases.

Anti-Platelet Function of the Non-Saponin Fraction from Panax ginseng C.A. Meyer (Korean red ginseng)

박화진,이만휘,박경미,남기열,박기현,Park, Hwa-Jin,Rhee, Man-Hee,Park, Kyeong-Mee,Nam, Ki-Yeul,Park, Ki-Hyun 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8

고려홍삼의 비사포닌분획(NSF)은 높은 농도의 트롬빈(0.2~$2{\mu}/ml$)에 의해 유도된 사람혈소판 응집반응을 강하게 억제했다. 그 응집정도는 $Ca^{2+}$ 길항제인 verapamil의 응집억제 정도보다 강했다. Verapamil은 혈소판 응집반응을 저해시키는 혈소판내 cAMP의 생성을 증가시켰지만, NSF는 cAMP의 생성증가에는 현저한 영향이 없었다. NSF는 $TXA_2$의 생성과 serotonin의 방출을 억제시키고 있었다. NSF는 혈소판막 인지질로부터 arachidonic acid의 방출을 억제하지 않고, arachidonic acid를 $TXA_2$로 전환시키는 어떤 경로를 차단하므로 혈전중의 치료 및 예방에 중요한 역할을 할 것으로 추측된다. The non-saponin fraction(NSF: lipophilic fraction) from Korean red ginseng showed an inhibitory activity in the aggregation of human platelets induced by a high dose of thrombin (0.2 to 2 u/ml), the extent of which was stronger than that of verapamil, a $Ca^{2+}$ antagonist. NSF did not affect the elevation of the intracellular cAMP concentration which inhibits platelet aggregation, as shown in the case of verapamil, but inhibited the production of $TXA_2$ and the release of serotonin. NSF does not inhibit the release of arachidonic acid from membrane phospholipids, but inhibits a certain pathway transforming arachidonic acid to $TXA_2$. NSF may play a critical role in preventing and curing thrombotic diseases.

유방암 환자에서 국소구역 재발과 관련된 인자

박인석 ( In Seok Park ),한세환 ( Se Hwan Han ),김기환 ( Ki Whan Kim ),배병노 ( Byung Noe Bae ),양근호 ( Keun Ho Yang ),곽금희 ( Geum Hee Gwak ),조현진 ( Hyun Jin Cho ),박경미 ( Kyeong Mee Park ),김지영 ( Ji Young Kim ),박성진 ( Su 대한임상종양학회 2011 Korean Journal of Clinical Oncology Vol.7 No.1

목적: 유방암 환자에서 국소구역 재발은 환자의 사망률을 증가시키는 요인이다. 본 연구는 유방 보존술을 받은 환자를 대상으로 국소구역 재발에 영향을 주는 인자를 알아보고자 진행 되었다. 방법: 단일 기관에서 유방 보존술을 받은 302명의 환자를 대상으로 후향적으로 의무 기록 및 조직 병리 보고서를 검토하여 국소구역 재발과 연관 있는 인자들을 분석 하였다. 결과: 302명의 환자 중 26명(8.6%)이 국소구역 재발을 보였고 중간 추적관찰기간 35.5개월 동안 18명(5.9%)이 국소재발을, 6명(2.0%)이 구역재발을, 그리고 2명(0.7%)이 국소구역 재발을 하였다. 각각의 인자들에 대한 단변량 분석에서 절제연 상태, HER2/neu 발현 양성, 광범위 관내상피 병소, 다발성 병변이 통계학적 유의한 인자였고 다변량 분석에서는 절제연 상태, HER2/neu 발현 양성, 다발성 병변이 유의한 인자였다. 결론: 침습 유방암에서 유방 보존술 후 국소구역 재발율을 낮추기 위해서는 수술 시 음성 절제연을 확보하고 보조 항암화학요법 및 방사선 치료를 고려해야 한다. 특히 다발성 병변을 가지고 있거나 HER2/neu 양성인 유방암은 국소 재발율이 높으므로 보조치료 후 국소 재발을 발견하기 위한 적극적 추적관찰이 필요 할 것으로 사료된다. Purpose: Significant proportion of breast cancer patients with locoregional recurrence eventually die of their disease. This study was performed to investigate the risk factors associated with locoregional recurrence of breast cancer after breast conserving surgery. Methods: We reviewed the medical records of 302 patients with breast cancer who underwent breast conserving surgery and analyzed the association between locoregional recurrences and clinico-pathological factors of each patients. Results: The 26 of 302 patients (8.6%) developed locoregional recurrence during a median follow-up of 35.5 months. 18 patients (5.9%) had local recurrence, 6 patients (2.0%) had regional recurrence and 2 patients (0.7%) had locoregional recurrence. On univariate analysis, positive margin (p<0.0001), HER2/neu positive, extensive intraductal component, multiplicity were statistically significant risk factors for locoregional recurrence of breast cancer. On multivariate analysis, positive margin status, HER2/neu positivity and multiplicity were significantly associated with locoregional recurrence after breast conserving surgery. Conclusion: Our study showed that adequate resection margin is the most important to reduce locoregional recurrence of breast cancer after breast conserving surgery. In addition, HER2/neu amplification or multiplicity increased the locoregional recurrence rate in breast cancer.