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Optimization of the human colorectal carcinoma antigen GA733‑2 production in tobacco plants
박세희,지건영,김현민,마상훈,박서영,도주희,오두병,강형식,심재성,정영희 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1
The colorectal carcinoma-associated protein GA733-2 is one of the representative candidate protein for the development of plant-derived colorectal cancer vaccine. Despite of its signifcant importance for colorectal vaccine development, low efciency of GA733-2 production limits its wide applications. To improve productivity of GA733-2 in plants, we here tested multiple factors that afect expression of recombinant GA733-2 (rGA733-2) and rGA733 fused to fragment crystallizable (Fc) domain (rGA733-Fc) protein. The rGA733-2 and rGA733-Fc proteins were highly expressed when the pBINPLUS vector system was used for transient expression in tobacco plants. In addition, the length of interval between rGA733-2 and left border of T-DNA afected the expression of rGA733 protein. Transient expression analysis using various combinations of Agrobacterium tumefaciens strains (C58C1, LBA4404, and GV3101) and tobacco species (Nicotiana tabacum cv. Xanthi nc and Nicotiana benthamiana) revealed that higher accumulation of rGA733-2 and rGA733-Fc proteins were obtained by combination of A. tumefaciens LBA4404 and Nicotiana benthamiana. Transgenic plants generated by introduction of the rGA733-2 and rGA733-Fc expression cassettes also signifcantly accumulated corresponding recombinant proteins. Bioactivity and stability of the plant-derived rGA733 and rGA733-Fc were evaluated by further in vitro assay, western blot and N-glycosylation analysis. Collectively, we here suggest the optimal condition for efcient production of functional rGA733-2 protein in tobacco system.
신축공동주택의 설내공기오염 평가를 위한 간이프로그램 개발
이윤규(Lee. Yun-Gyu),한길원(Han Kil-Won),도주희(Doh Joo-Hee) 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1
The quantity and harmfulness of pollutants that originate in architectural materials increase more and more. At present, we need ventilation system that can effectively remove extremely small quantities of noxious pollutant and basic research that institutes guidelines to prevent accumulation pollutants indoors.<br/> It is time to request "the Healthy Buildings" for agreeable residential environment. So, the aims of this study are to establish developing synthetic design supporting program relation to ventilation system and coordinating information of indoor air.