회내족 대상자의 스쿼트 동안 능동적 족궁 지지가 하지근육의 근전도 활성도에 미치는 영향

남기석,박지원,Nam, Ki-Seok,Park, Ji-Won 대한물리치료학회 2010 대한물리치료학회지 Vol.22 No.5

Purpose: The purpose of this study was to identify the effect of active foot arch support on the muscles of lower extremity electromyographic activity during squat exercise in persons with pronated foot. Methods: The study subjects were 16 persons with pronated foot. They have no history of surgery in lower extremity and trunk and limitation of range of motion or pain when performing squat exercise. Each subject was measured the navicular drop (ND) to determine the pronated foot. And then the subjects were asked to perform three repetitions of a $90^{\circ}$ knee flexion squat in both conditions which are 1) preferred squat and 2) squat with active foot arch support. Results: Paired t-test revealed that squat with active foot arch support produced significantly greater EMG activities in abductor hallucis (p=0.00), proneus longus (p=0.03) and gluteus medius (p=0.04) than preferred squat. But the EMG activities of tibialis anterior, vastus medialis oblique and vastus lateralis were not showed significantly different between the both squat conditions. Conclusion: The findings of this study suggest that active foot arch support during squat increase the activities of lower extremity muscles which are the abductor hallucis, proneus longus and gluteus medius. Also, the abductor hallucis which is one of the planter intrinsic muscle and peroneus longus play a role in support of the foot arch and active foot arch support induced the increase of the activity of gluteus medius. Therefore active foot arch support can change the lower extremity biomechanics as well as passive foot support such as foot orthotics and taping.

뇌성마비 아동에서 노력성 폐활량에 영향을 미치는 요인 분석에 관한 연구

남기석,이혜영,Nam, Ki Seok,Lee, Hye Young 대한물리치료학회 2013 대한물리치료학회지 Vol.25 No.4

Purpose: Children with cerebral palsy generally have a high incidence of respiratory problem, resulted from poor coughing, airway clearance problem, respiratory muscle weakness, kyphoscoliosis and so forth. The purpose of this study is to investigate the possible factors that can be affected to forced vital capacity (FVC) in children with cerebral palsy. Methods: Total thirty six children with diplegic and hemiplegic cerebral palsy were recruited in this study. They were evaluated by general demographic data (i.e., age, gender, body mass index (BMI)) and variables related to respiratory functions (i.e., chest mobility, waist mobility, maximal phonation time, and maximum inspiratory/expiratory pressure (MIP/MEP)). The correlation between forced vital capacity and the rested variables were analyzed, and multiple regression with stepwise method was conducted to predict respiratory function, in terms of FVC as the dependent variable, and demographic and other respiratory variables as the independent variable. Results: FVC showed a significant correlation with waist mobility (r=0.59, p<0.01), maximal phonation time (r=0.48, p<0.05), MIP (r=0.73, p<0.01), and MEP (r=0.60, p<0.01). In addition, the multiple regression analysis model indicated that FVC could be predicted by the assessment of each waist mobility and MIP. Conclusion: These finding suggest that respiratory function is related to body size and respiratory muscle strength, and that BMI, waist mobility, and MIP can be predictable factors to affected respiratory function in term of FVC.

금형의 확산피복기술 현황

남기석,이기정,이만수,권식철,Nam, Gi-Seok,lee ki-jung,lee man-su,kwon sik-chul 재료연구소 1993 機械와 材料 Vol.5 No.2

동적 스쿼트 운동시 탄력밴드를 이용한 저항방향이 내측광근/외측광근 근전도 활성비에 미치는 영향

남기석,Nam, Ki-Seok 대한물리치료학회 2008 대한물리치료학회지 Vol.20 No.3

Purpose: The purpose of this study was to identify the effect of anterolateral (45$^{\circ}$) and lateral (90$^{\circ}$) direction resistance, with using an elastic band, on the electromyographic(EMG) activity ratio of the vastus medialis oblique (VMO) and the vastus lateralis (VL) during squat exercise. Methods: The study subjects were 19 active people with no history of patellofemoral pain, limitation of range of motion or pain when performing squat exercise. A 'repeated measures within subjects' design was used. The subjects were asked to perform three repetitions of a 90$^{\circ}$ knee flexion squat exercise with anterolateral (45$^{\circ}$) and lateral (90$^{\circ}$) resistance and without resistance, respectively. The EMG activity of the VMO and VL were recorded by surface EMG electrodes and the results were normalized by the % MVIC value. Results: Repeated measures ANOVA's revealed that squat exercise with anterolateral (45$^{\circ}$) resistance produced significantly greater VMO/VL EMG activity ratio than that with lateral (90$^{\circ}$) resistance and without resistance (p=.013). Yet the result of contrast testing revealed that squat exercise with lateral (90$^{\circ}$) resistance showed no significant difference of the VMO/VL EMG activity ratio, as compared with squat exercise without resistance (p>0.05). Conclusion: The findings of this study suggest that squat exercise combining anterolateral (45$^{\circ}$) resistance can contribute positively to the patients with patellofemoral pain as they increase the VMO/VL EMG activity ratio.

금융 MicroSD와 무인중 서명 암호화 기법을 통한 안전한 모바일 신용카드결제 프로토콜에 관한 연구

남기석(Ki Seok Nam),박민호(Min Ho Park) 한국통신학회 2015 한국통신학회 학술대회논문집 Vol.2015 No.1

컴퓨터 통신망에서 ARQ 프로토콜 방식 비교

남기석(Ki-Seok Nam),곽경섭(Kyung S. Kwak) 대한전자공학회 1991 대한전자공학회 학술대회 Vol.1991 No.6

운동학습에 의한 왼쪽 하전두영역의 분할비등방성의 변화

박지원,남기석,Park, Ji-Won,Nam, Ki-Seok 대한물리치료학회 2010 대한물리치료학회지 Vol.22 No.5

Purpose: This study was to delineate the structural change of neural pathway after sequential motor learning using diffusion tensor imaging (DTI). Methods: The participants were 16 healthy subjects, which were divided by training (n=8) and control (n=8) group. The task for the training was the Serial Reaction Time Task (SRTT) which was designed by Superlab program. When the 'asterisk' shows up in the 4 partition spaces on the monitor, the subject presses the correct response button as soon as possible. The training group participated in the training program of motor learning with SRTT composed of 24 digits pattern in one hour per daily through 10 days during 2 weeks. Results: In the behavioral results the training group showed significant changes in the increase of response number and the reduction of response time than those of the control group. There was significant difference in the left inferior frontal area in the fractional anisotropy (FA) map of the training group in DTI analysis. Conclusion: Motor sequential learning as like SRTT may be needed to the learning of language and visuospatial processing and may be induced for the experience-dependent structural plasticity during short period.

Ferric iron reductase activity of LuxG from Photobacterium leiognathi

이의호,남기석,이선광,오동현,이찬용,Lee, Eui Ho,Nam, Ki Seok,Lee, Seon Kwang,Oh, Eugeney,Lee, Chan Yong The Microbiological Society of Korea 2016 미생물학회지 Vol.52 No.4

본 연구에서는 발광 세균에 존재하는 LuxG 단백질의 효소학적 성질을 알아내기 위하여 Photobacterium leiognathi ATCC 25521의 luxG 유전자를 중합효소연쇄반응으로 증폭시켜 T5 프로모터와 6X His-tag 시스템을 지닌 pQE 벡터에 삽입시킨 재조합플라스미드를 제조하여 대장균에 형질전환 후 과발현시켜 단백질을 분리, 정제 하였다. 정제된 단백질의 효소학적 실험 결과, 이 단백질은 FMN과 NADPH 기질에 대한 ferric iron reductase의 기능을 갖고 있음을 확인하였으며 이들 기질에 대한 효소 활성도 상수 $K_m$ 및 $V_{max}$ 값을 결정하였다. In order to identify the biochemical characteristics of LuxG, the luxG gene from bioluminescence bacteria of Photobacterium leiognathi ATCC 25521 was isolated by PCR-Amplification and inserted into pQE30 vector containing the T5 promoter and 6X His-tag system. The resulting recombinant plasmid was transformed into Escherichia coli to over-express the luxG gene and purify the gene product. The purified LuxG protein demonstrated ferric iron reductase activity and the kinetic parameters of $K_m$ and $V_{max}$ for FMN as well as the NADPH substrates of ferric iron reductase were determined, respectively.

아미노-말단 리보플라빈 생성효소 단백질의 형광 특성

김류련,이정환,남기석,고경원,이찬용,Kim, Ryu-Ryun,Yi, Jeong-Hwan,Nam, Ki-Seok,Ko, Kyung-Won,Lee, Chan-Yong 한국미생물학회 2011 미생물학회지 Vol.47 No.1

리보플라빈 생성효소(riboflavin synthase)는 기질인 두 분자의 6,7-dimetyl-8-ribityllumazine과 결합 후, 4-탄소 단위(4-carbon unit)의 자리 옮김 반응을 거쳐 한 분자의 리보플라빈과 한 분자의 pyrimidine 유도체를 형성하는 반응을 촉매한다. 대장균(Escherichia coli) 리보플라빈 생성효소의 아미노-말단 도메인 절반(N-terminal domain half)과 카복시-말단 도메인 절반(C-terminal domain half)은 매우 유사한 내부 자체 아미노산 서열(intra-molecular amino acid sequence)을 갖는다. 아미노-말단 영역 리보플라빈 생성효소(N-RS) 단백질의 구조와 형광 특성을 알아보기 위하여 중합효소 연쇄 반응과 위치지정 돌연변이를 통하여 10개 이상의 돌연변이 아미노-말단 리보플라빈 생성효소 단백질을 코드 하는 유전자를 증폭시켜 pQE30 벡터에 삽입한 재조합 플라스미드를 제조하여, 과발현시킨 후 분리 정제하였다. 대부분의 아미노-말단 도메인 리보플라빈 생성효소의 돌연변이 단백질들은 야생형과 같이 형광성 리간드인 6,7-dimetyl-8-ribityllumazine 혹은 리보플라빈과 결합할 수 있는 능력을 지니고 있었으나, N-RS C47D, N-RS ET66,67DQ 돌연변이 단백질의 경우는 리간드와의 결합능력이 현저히 떨어져 형광을 띠지 않았다. 대부분의 돌연변이 단백질들의 형광 세기는 야생형 단백질(N-RS wt)보다 낮았으나, N-RS C48S는 예외적으로 야생형 단백질에 비해 2배 이상의 형광세기를 가졌다. 이와 같은 결과를 바탕으로 리보플라빈 생성효소와 형광성 리간드 사이의 상호작용을 예측 할 수 있으며, N-RS C48S 돌연변이 단백질의 형광성을 활용하여 효과적으로 효소 저해제를 발굴할 수 있는 고속다중 스크리닝 법(high-throughput screening system)으로써 활용될 수 있을 것이다. Riboflavin synthase catalyzes the formation of one molecule of each riboflavin and 5-amino-6-ribitylamino-2,4-pyrimidinedione by the transfer of a 4-carbon moiety between two molecules of the substrates, 6,7-dimetyl-8-ribityllumazine. The most remarkable feature is the sequence similarity between the N-terminal half (1-97) and the C-terminal half domain (99-213). To investigate the structure and fluorescent characteristics of the N-terminal half of riboflavin synthase (N-RS) in Escherichia coli, more than 10 mutant genes coding for the mutated N-terminal domain of riboflavin synthase were generated by polymerase chain reaction. The genes coding for the proteins were inserted into pQE vector designed for easy purification of protein by 6X-His tagging system, expressed, and the proteins were purified. Almost all mutated N-terminal domain of riboflavin synthases bind to 6,7-dimethyl-8-ribityllumazine and riboflavin as fluorescent ligands. However, N-RS C47D and N-RS ET66,67DQ mutant proteins show colorless, indicating that fluorescent ligands were dissociated during purification. In addition, most mutated proteins show low fluorescent intensity comparing to N-RS wild type, whereas N-RS C48S posses stronger fluorescent intensity than that of wild type protein. Based on this result, N-RS C48S can be used as the tool for high throughput screening system for searching for the compound with inhibitory effect for the riboflavin synthase.

치매예방 프로그램 운영 및 효과검증 연구

황옥남(Hwang Ok Nam),윤숙례(Yoon Sook Rye),황혜연(Hwang Hye Yeon),남기석(Nam Ki Seok) 한국재활간호학회 2002 재활간호학회지 Vol.5 No.2

The objective of this study was to apply a dementia prevention program to the subjects who were suspected of dementia and test its effect.<br/> This study was one group pretest-posttest design. The dementia prevention program was applied for 20 weeks to the 19 subjects who were suspected of dementia after a screen test among 638 subjects enrolled in 10 senior citizen's centers in G city. The scores of measured before and after the program.<br/> The data collected were analyzed using a SPSS (statistical analysis system) program, and frequencies, averages and standard deviations were obtained. The differences of the scores of the pretest and posttest were analyzed with Mann-Whitney test and sign test. <br/> The results were as follows:<br/> 1. After the program, the average score of self-efficacy was increased from 75.31 (standard deviation 11.99) to 84.26 (standard deviation 13.92).<br/> 2. There are no differences between the average scores of physical and psychological quality of life, however, the average score of social quality of life was slightly increased from 2.25 (standard deviation 0.40) to 2.53 (standard deviation 0.43), and the overall score of social quality of life was low.<br/> 3. The average score of cognition was significantly increased from 21.00 (standard deviation 2.60) to 24.58 (standard deviation 3.37), thus, it was found that the program was effective to improve the cognition level.<br/> 4. The score differences of self-efficacy, quality of life, cognition between the pretest and posttest were statistically significant.<br/> 5. The score differences of quality of life between the pretest and posttest were found to be dependent on marital status, exercise and regular chechup yes or no.<br/> In conclusion, the program was effective to prevent the subjects suspected of dementia from dementia.<br/> <br/>