초음파가 흰쥐의 좌골신경 압좌손상 후 척수내 Neural Cell Adhesion Molecules의 발현에 미치는 영향

김현애,한종만,Kim, Hyun-Ae,Han, Jong-Man 대한물리치료학회 2007 대한물리치료학회지 Vol.19 No.2

Purpose: This study aimed to compare the effect on nerve regeneration of ultrasound irradiation in rats with peripheral nerve injury. Methods: To investigate alterations of the NCAM immunoreactivity in non-crushed part and crushed part of the spinal cord, the unilateral sciatic nerve of the rats were crushed. The expression of NCAM was used as the marked of peripheral nerve regeneration, and also plays an important role in developing nerve system. Experimental animals were sacrificed by perfusion fixation at post-injury 1, 3, 7, 14 days after ultrasound irradiation. The pulsed US was applied at a frequency of 1MHz and a spatial average-temporal average Intensity of 0.5W/of (20% pulse ratio) for 1 mins. The Luxol fast blue-cresyl violet stain were also done to observe the morphological changes. Results: Alteration of NCAM immunoreactivity in the crushed part and the non-crushed part of lower lumbar spinal cord were observed. NCAM-immunoreactivity cells were some increased in the dorsal horn lamina I, III and cell ventral horn at 1 day after unilateral sciatic nerve injury. However, there was not significant difference in the relationship between crushed part and non-crushed part. NCAM-inmmunoreactivity was remarkably increased at 3 days after unilateral sciatic nerve injuryin the gray matter and white matter. NCAM-immunoreactivity was increased in the ventral horn and post horn of experimental crushed part. Also, NCAM-immunoreactivity in large motor neurons in ventral horns lamina VIII, IX were increased at 7 days after unilateral sciatic nerve injury. At 14 days after sciatic nerve crushed injury, there was no significant difference. All group were decreased for 14 days. In the time course of NCAM expression, all groups showed a significant difference at 3day groups(p<0.05). Whereas, CC group was noted a significant difference between 3day and 7 day group respectively. In NCAM expression, there were significantly increased in all group. In the relationship between CNC group and ENC group, significant difference was detected among 3, 7, 14 day group(p<0.05). The difference between CC group and ENC group were noted in all groups(p<0.05). Conclusion: It is consequently suggested that the effects of the ultrasound irradiation may increase the NCAM immunoreactive neurons and glial cell in the spinal cord after unilateral sciatic nerve crushed injury. Therefore, the increased NCAM immunoreactivity in the spinal cord may reflect the neuronal damage and healing process induced by a ultrasound irradiation after peripheral nerve injury in rat.

Stimulatory Effect of Dietary Red Ginseng on Epidermal Hydration and Ceramide Levels in Ultraviolet-Irradiated Hairless Mice

김현애,Inngyung Oh,박경호,Na-Mi Kim,Jae-Ho Do,조윤희 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.4

Ultraviolet (UV) irradiation induces skin dryness, largely by disruption of the epidermal barrier. In a search for dietary and plant compounds that would protect against skin dryness, we investigated the dietary effect of red ginseng (the steamed root of Panax ginseng C.A. Meyer) on epidermal levels of hydration and ceramides, the most important lipids for maintaining the epidermal barrier, in UV-irradiated mice. Albino hairless mice were fed either control diets (group UV [UV-irradiated control]) or diets with 0.5% (group H0.5) or 1% (group H1.0) red ginseng extract for 5 weeks in parallel with UV irradiation. A normal control group (group C) was fed a control diet without UV irradiation for 5 weeks. Skin dryness in group UV, as assessed by epidermal levels of hydration and ceramides, was significantly lower than those in group C. With no differences in food intake and weight gains among groups, epidermal levels of hydration and ceramides in group H0.5 were similar to those in group C. In addition, protein expression of serine palmitoyltransferase (SPT), a key enzyme involved in de novo ceramide synthesis, was increased in group H0.5. However, epidermal levels of hydration and ceramides in group H1.0 did not differ from those in group UV, in which ceramidase, an enzyme involved in ceramide degradation, was highly expressed. In conclusion, we demonstrate that dietary supplementation of 0.5% red ginseng protects skin from UV-induced dryness with an accumulation of ceramides due to elevated expression of SPT protein.

20대 남성 비만인의 자세에 따른 가슴우리 확장과 폐기능 특성분석

김현애 ( Hyeun Ae Kim ),서교철 ( Kyo Chul Seo ),임상완 ( Sang Yoan Yim ),김희탁 ( Hee Tak Kim ),김경 ( Kyoung Kim ) 대한물리의학회 2011 대한물리의학회지 Vol.6 No.3

Purpose:The purpose of this study were to determine whether changes of position might effect the chest expansion and pulmonary function of the 20s men obesity. Methods:Thirty subjects with obesity(M30, % fat>25.0) and thirty normal subjects(M30, % fat<24.9) were participated in experiment. Subjects were assessed according to position changes(supine position, 45° lean sitting position, 90° sitting position) using chest length(chest length for resting, chest expansion) and pulmonary function (Tidal volume, Inspiratory capacity, Vital capacity, Inspiratory reserve volume, Expiratory reserve volume) by the CardioTouch 3000S(BIONET, USA). Repeated measure ANOVA was used to compare each region data of chest length and pulmonary function according to changes of position with obesity and normal subjects. Results:These findings suggest that the obesity can be appear to low chest expansion and pulmonary function than normal subjects on position method, In comparison of three experimental position, supine position was more low. Conclusion:This study showed position of the obesity appear low chest expansion and function of pulmonary volume than normal subjects, and thus it indicates that the pulmonary function of the obesity will be suggest objective respiratory data through the exercise program.

일부 의과대학생들의 사망진단서 작성의 정확성 평가

김현애(Hyun-Ae Kim),김건엽(Keon-Yeop Kim),감신(Sin Kam),오경재(Gyung-Jae Oh),신민호(Min-Ho Shin),손석준(Seok-Joon Sohn),김순영(Soon-Young Kim),남해성(Hae-Sung Nam) 한국농촌의학 지역보건학회 2010 농촌의학·지역보건 Vol.35 No.1

의과대학 본과 4학년 학생들의 사망진단서의 작성 능력을 살펴보고자, 2007년 5월부터 8월까지 충청도, 전라도, 경상도의 4개 의과대학 본과 4학년 학생 380명을 대상으로 설문조사를 실시하였다. 설문지는 사망진단서 작성을 위한 10개의 사례들과 사망진단서 작성 양식, 그리고 사망진단서 작성과 관련된 교육 경험에 관한 문항들로 구성되었다. 교육 유형별 사망진단서 작성 정확도를 평가하기 위해 학생들이 기술한 사인들이 기준사인과 일치되는 정도와 기재내용의 오류 등을 파악하였다. 설문 미응답자 61명을 제외한 232명의 분석결과는 다음과 같다. 10개 사례에 대한 4단계 사망원인 평균 일치개수 즉 CD는 9.6±3.8이었다. 4단계 사망원인들 중 최하단에 기재한 원사인의 평균 일치 개수 즉 UC1은 4.8±1.7이었고, 학생들이 기술한 4단계 사망원인들 중에서 사인분류사에 의해 선정된 원사인의 평균 일치 개수 즉 UC2는 5.6±1.5이었다. UC1과 UC2는 사례중심 교육집단이 이론교육집단 보다 높았다. 주오류의 세부 내용별로 살펴보면 ‘선행사인 미기입’의 오류를 가진 경우(78.9%)가 가장 많았으며, 다음으로 ‘한 칸에 두개 사인 기입’(48.3%), ‘Ⅱ부에 Ⅰ부 기입‘(43.1%)등의 오류를 범하는 경우 등이 많았다. 주오류와 부오류 모두 사례중심교육집단이 이론교육집단보다 평균 오류의 수가 더 적었다. Objectives: The purpose of this research was to evaluate the ability of completing death certificates among medical students. Methods: The self-administered questionnaires were completed, during May to August 2007, by 380 medical students in senior. The questionnaire was composed of 10 cases to write the death certificate. The cause-of-deaths written by students were compared with the gold standards and their errors in the certificates also evaluated. Results: Mean agreement score for 10 underlying cause-of-deaths completed on the lowest line of part I in the death certificate (UC1) was 4.8±1.7, and for underlying cause-of-death selected by a coder of the death certificates (UC2) was 5.6±1.5. The UC1 and UC2 were significantly higher among the students having the case-oriented education for death certificate than others. For the major errors in the certificates completed by students, the students having the error with no antecedent cause were highest, the error with two or more conditions secondly highest. Mean number of errors was significantly lower in the case-oriented education group than others. Conclusions: Errors are common in the death certificates completed by medical students in senior. The accuracy of death certification may be more improved with the case-oriented education than the traditional method.

비타민 C, Silicon, 철분, Proline 및 Lysine의 처리가 피부 섬유아세포의 증식 및 Collagen Ⅰ과 Ⅲ의 발현에 미치는 효과 비교

김선아(Sun-Ah Kim),이진아(Jin-Ah Lee),김정민(Jung-Min Kim),김현애(Hyun-Ae Kim),김영애(Young-Ae Kim),윤혜정(Hye-Jeong Yun),조윤희(Yunhi Cho) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.11

본 연구에서는 HS27 피부 섬유아세포에 비타민 C, silicon, 철분, proline 및 lysine을 혈청 및 피부 내 농도 범위로 처리한 후 세포의 증식을 비롯하여 콜라겐 Ⅰ과 Ⅲ의 mRNA 및 단백질의 발현을 비교하였다. 비타민 C는 처리 기간 및 농도와 무관하게 세포 증식 및 mRNA 발현에 영향을 미치지 않았으며 콜라겐 type Ⅰ 및 Ⅲ의 단백질 발현은 각각 5~50 μM, 10~50 μM 농도에서 5일 처리 시 683.9%, 182% 이상으로 발현을 증가시켰다. Silicon의 3일 및 5일 농도별 처리는 세포증식과 콜라겐 mRNA 및 단백질 발현을 다소 감소시키거나 영향을 미치지 않았다. 철분의 3일 및 5일 처리는 혈청내 농도(11~35 μM)에서 콜라겐 mRNA 발현에 영향을 미치지 않았으며 세포 증식 및 콜라겐 단백질 발현을 증가시켰다. 또한 철분의 5~30 μM의 농도 처리는 5일 처리보다 3일 처리 시 발현수준이 훨씬 컸다. 콜라겐의 구성 아미노산인 proline 및 lysine은 섬유아세포의 증식을 증가시켰다. 따라서 단기간의 콜라겐의 합성 증가는 철분의 섭취가 적합하나 장기간의 섭취에는 비타민 C의 섭취와 더불어 철분의 섭취가 필요한 것으로 여겨진다. In the dermis, fibroblast plays an important role in the turnover of the dermal extracellular matrix. Collagen Ⅰ and Ⅲ, which are the most important dermal proteins of the extracellular matrix, function as a stabilizing scaffold of dermal connective tissues, as well as a regulator of differentiation and migration of dermal cells. In this study, we investigated the effect of various nutrients, such as ascorbic acid, silicon, Fe, lysine and proline which function as cofactors or building blocks on collagen synthesis. When the physiological concentrations of ascorbic acid (0~100 μM), silicon (0~50 μM), Fe (0~50 μM), lysine (0~150 μM) and proline (0~300 μM) were treated at HS27 for either 3 or 5 days, 5 day treatment of ascorbic acid at the low concentration (5~10 μM) increased the expression of collagen Ⅰ and Ⅲ protein by 115~1300% without increasing cell proliferation. 3 or 5 days treatment of Fe increased the expression of collagen Ⅰ and Ⅲ proteins up to 323% in parallel with cell proliferation by 164%. However, cell proliferation and expression of collagen Ⅰ and Ⅲ protein in silicon treated HS27 did not differ. Proline and lysine only increased cell proliferation up to 247.9%. Taken together, we demonstrate that the physiological concentrations of ascorbic acid and Fe enhance the expression of collagen Ⅰ and Ⅲ protein for treatment of 3 or 5 days.

초등학교 과학 수업에서 드러나는 학생의 행위주체성: 교수 학습 맥락을 중심으로

김현애 ( Hyunae Kim ),강은희 ( Eunhee Kang ),김희백 ( Heui Baik Kim ) 韓國生物敎育學會 2015 생물교육 Vol.43 No.3

This study aims to explore how students expressed their agency in the science classroom and the features of teaching and learning contexts that stimulate such agency. We developed several biology lessons for two fifth-grade classes in an elementary school located in Seoul. The lesson topics covered on diseases - caused by mold, viruses, germs, and dust mites - and its prevention. During lessons one and two, the students actively discussed the topics based on their funds of knowledge, grounded in their experiences outside of school. In lessons three and four, students made video clips, slogans, posters, and newspapers and then campaigned to inform the public. Data from participant observations, individual interviews, and students’ artifacts were qualitatively analyzed based on grounded theory. According to the results, students expressed agency in four ways: 1) They created an objective for their disease topic; 2) they purposefully selected and utilized available resources; 3) they planned and launched a campaign to educate others around them; and 4) they used their scientific knowledge to carefully take preventative measures in their everyday lives. The most salient feature of the teaching and learning context that stimulated students’ agency was the lesson contents which were directly connected to their lives. Furthermore, as the teacher shared her authority with the students, encouraging them to utilize their funds of knowledge and available resources, the students could enhance their authority to realize their objective. Based on these findings, this paper discusses the educational implications for promoting students’ agency by taking a closer look at teaching and learning contexts that could position students as autonomous agents.

실크 단백질 Sericin 및 Fibroin의 식이 공급이 아토피 피부염 동물 모델 NC/Nga Mice의 혈장과 표피의 유리 아미노산 함량에 미치는 영향

김현애(Kim Hyunae),박경호(Park Kyung-Ho),여주홍(Yeo Joo-Hong),이광길(Lee Kwang-Gill),정도현(Jeong Do-Hyeon),김성한(Kim Sung-Han),조윤희(Cho Yunhi) 韓國營養學會 2006 Journal of Nutrition and Health Vol.39 No.6

국내 독서복지 실태 및 개선방안 : 취약계층 아동ㆍ청소년을 중심으로

김현애 ( Kim Hyunae ) 한국독서학회 2022 독서연구 Vol.- No.62

본 연구는 국내 독서복지 정책 현황과 취약계층 아동ㆍ청소년에 대한 독서복지 실태 파악 및 개선방안을 모색하여 독서복지 정책 실행의 기초자료를 제공하기 위해 진행되었다. 연구결과 첫째, 국내 공공기관에서 진행되는 취약계층 아동ㆍ청소년 대상의 독서복지 사업은 공공도서관이나 아동복지시설, 학교 등에서 진행되는 대상별, 상황별 독서 프로그램 운영과 취약계층을 위한 양서지원 및 대체 자료 제작 지원에 초점이 있었다. 둘째, 프로그램 운영 면에서는 독서를 통한 심리·정서적 안정지원 프로그램이나 독서문화향유 기회 제공 프로그램이 대부분이며, 독서를 통한 기초학력 및 문해력 증진을 위한 수업도 일부 진행되고 있었다. 셋째, 사업성과 면에서는 전반적인 만족도는 높았지만, 대부분 부처별 사업으로 한시적으로 운영되는 점과 내용구성이 독서문화체험에 치우쳐 독서흥미 유발에 그치는 점, 취약계층의 유형과 상황에 대한 세심한 고려가 미흡한 점 등이 문제로 지적되었다. 독서복지 실행의 내실화를 위한 개선방안 제언은 다음과 같다. 첫째, 대상 맞춤형 자료개발 및 독서 프로그램 제공 확대와 전문인력 훈련이 필요하다. 둘째, 독서복지 정책 추진을 위한 국가 차원의 부처간 협력체계 구축과 공동목표 설정을 통한 실효성 있는 사업실행이 필요하다. This study aims to examine the status of Korea’s reading welfare policy and of reading welfare programs for vulnerable children and youth, and to find ways to improve them. The results of this study are as follows. First, reading welfare programs for vulnerable children and youth in domestic public institutions include public libraries, child welfare facilities, the operation of reading programs by subject and situation, and book support for the vulnerable. Second, regarding program operations, most programs that support psychological and emotional stability through reading or programs that provide opportunities to enjoy reading culture are being implemented. Third, the overall satisfaction with work performance is high, but most are operated as temporary tasks by department, and there is a limit to continuous reading because the content structure is biased toward the reading culture experience. The lack of careful consideration for the types and situations of the vulnerable is also problematic. The suggestions for improvement measures are as follows. First, it is necessary to develop target materials for the vulnerable, expand reading programs, and nurture professional manpower. Second, the government should provide effective reading welfare through linkage and cooperation with other ministries for the implementation of reading welfare, and develop it as a common goal.

전문대학 광고관련학과 교육과정에 대한 전문가 및 졸업생들의 인식에 관한 연구

김현애 ( Hyun-ae Kim ) 한국고등직업교육학회 2008 한국고등직업교육학회논문집 Vol.9 No.4

The purpose of college education related advertising is to train advertising professionals. To achieve this goal, colleges are emphasizing practical hands-on approach to education in the advertising field. This research will find out how much practical knowledge and ability graduates of advertising college programs have in their respective fields and how the advertising industry judges the quality of graduates in terms of their knowledge and ability in advertising. From the research we will provide the basic data from which colleges can develop an effective advertising curriculum. To properly train advertising professionals, we have selected the following fields of study as necessary education needed to succeed in the ad field: critical theory, creative ability, practical ability, technical skills, basic knowledge of humanities and foreign language skills. The research has shown that advertising professionals acknowledge the lack of knowledge and ability of college graduates of advertising departments as do the graduates themselves when working in the professional field. Based on data provided by graduates currently working in advertising fields, there is a need for well-trained graduates from advertising related departments. In order to achieve this we need to restructure the curriculum of the colleges to be more effective in educating future advertising professionals.

한국무용 3단 디딤새 동작의 운동학적 및 하지근 근전도 분석

김현애 ( Hyun Ae Kim ),최영은 ( Young Eun Choi ) 한국리듬운동학회 2010 한국리듬운동학회지 Vol.3 No.1

This study examines time variables, changes in hip joint angle, changes in knee joint angle, changes in ankle joint angle, changes in knee joint angular velocity, and changes in knee joint angular velocity according to the level of proficiency of the basic three footstep motions in Korean traditional dance. Moreover, the average values of integral calculus electromyogram to the major lower leg muscles in the three footstep motions are calculated to draw the activity of muscles. The results are as follows: 1. There was no significant difference in time variables between proficient ones and unskilled ones of the three footstep motions. 2. As to changes in hip joint angle, it is thought that proficient ones maintain the upper body erect while stepping forward without overloading the hip joint, and with soles of feet, knees, and the body in a straight line, which are desirable motions, while unskilled ones could not maintain the right position with the body shaking while the center of gravity was moving from calcaneus to longitudinal metatarsal. 3. As to changes in knee joint and ankle joint angles, there was no significant difference between proficient ones and unskilled ones. 4. As to hip joint angular velocity, proficient ones had the hip joint angular velocity drastically lowered so that the calcaneus can sustain the phalange, which results from the vertical descent while treading with metatarsals. 5. As to knee joint angular velocity, proficient ones showed changes in angular speed upon treading with metatarsals, which is because of the bending of knee joints and huge effect on knee joints during the footsteps. Motions of feet seem to be maintained in a stable and constant manner. On the contrary, the changes in angular velocity were reduced while unskilled ones trod with longitudinal metatarsals, which caused bending of knee joints but no effect on knee joints during the three footstep motions. 6. As to changes in ankle joint angular velocity, the drastic speed reduction of the hip joint angular as both proficient and unskilled ones could tread with phalange in support of calcaneus resulted from the vertical descent while treading with metatarsals. 7. As to physical activity of ankle joints and changes in muscle contraction, there was no significant difference in relation to ankle angle, ankle angular velocity, Tibialis Anterior muscle, and outer gastrocnemius muscle while contraction of inner gastrocnemius muscle and soleus was active and accelerated, which indicates that the use of soleus muscle and inner gastrocnemius muscle is important in the three footstep motions.