Acanthamoeba culbertsoni에서 분리한 세포독성 물질의 특성연구

김태우,Kim, Tae-Ue 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.5

병원성 자유생활 아메바인 Acanthamoeba culbertsoni에서 순수 분리한 세포독성 물질 (cytolytic substance)을 가지고 몇 가지 특성을 알아보았다. 세포독성 물질의 아미노산 조성을 분석한 결과 serine이 26개로 비교적 많이 존재하는 것을 알 수 있었으며, 면역시켜 얻은 immune serum은 표적세포에 대한 세포독성 물질의 cytolytic activity를 억제하는 효과를 보였고 세포독성 물질이 이 병원성 아메바의 세포막에 존재하는 것을 간접형광 항체법으로 관찰하였다. 전자현미경으로 관찰한 결과 표적세포에 아메바가 filopodia를 내어 접촉하는 것을 관찰할 수 있었으며, 표적세포에 세포독성 물질의 처리시 3시간 후에는 표적세포의 microvilli가 점차로 감소하고 많은 수포가 생겼으며 24시간 후에는 세포막이 pore가 생기면서 녹아버리는 현상을 관찰할 수 있어 표적세포와의 접촉시 세포독성 물질을 분비하여 세포를 사멸시킨다는 작용 mechanism을 제시할 수 있었다. The cytolytic substance of the pathogenic free living amoeba, Acanthamoeba culbertsoni, has been claimed to be closely related to meningoencephalitis. Amino acids composition of the purified cytolytic substance revealed relatively high content of serine. The cytolytic activity on target CHO cells by the cytolytic substance was greatly decreased when rabbit immune serum added. The cytolytic substance proved to be present on the surface membrane of A. culbertsoni, which was demonstrated by indirect immunofluorescent assay. And electron microscopic observation showed the filopodia of the amoeba contacting with the target cell, decrease in the number of microvilli in the target cells and degeneration of the cell membrane and organelles 24 h after the cytolytic substance treatment, causing the death of the target cells. These results suggest such an action mechanism that the pathogenic amoeba might damage the target cell membrane through the proteinase activity of the cytolytic substance on contact resion of the target cells when the amoeba attacks the mammalian target cells.

사회 속의 의료, 의료 속의 사회 : 한국의 한의학과 중국의 중의학에 대한 의료인류학적 고찰

김태우,한창호,Kim, Tae-Woo,Han, Chang-Ho 대한한방내과학회 2012 大韓韓方內科學會誌 Vol.33 No.2

Objectives : One of the fundamental premises of medical anthropology is the interconnectedness of medicine and society. Recent ethnographies of medicine demonstrate that the interconnectedness of the social and the medical not just evokes relatedness of the two parties, but also emphasizes the agency of the constituents, mutually shaping and being shaped. Against this backdrop, this study attempts to anthropologically investigate Korean medicine in South Korea and traditional Chinese medicine (TCM) in China. Methods : The findings are based on anthropological studies of East Asian medicine employing long-term fieldwork about Korean Medicine and Traditional Chinese Medicine. Results : TCM is characterized by standardization, hospitalization, and scientization, by which simplification, collectivization, and biomedicalization prevail in contemporary traditional medicine in China. In contrast, Korean medicine is characterized by diversity, care delivery by individual private clinics, and a considerable distance from biomedicine. To understand the divergence of the two East Asian medicines, one should consider the social contexts intervening into the medical contents, such as the role of the state and dominant discourses in given historical periods. Conclusions : Korean medicine in South Korea and TCM in China demonstrate well the hybridity of the social and the medical, suggesting that, for more comprehensive understanding of the medical, the social should be paid attention to.

건설안전실무-건설현장 감성 안전

김태우,Kim, Tae-U 대한산업안전협회 2008 안전기술 Vol.123 No.-

동의보감(東醫寶鑑)의 신형장부도(身形臟腑圖)와 정기신(精氣神)의 관련성(關聯性)에 관(關)한 연구(硏究)

김태우,윤종화,Kim, Tae-U,Yun, Jong-Hwa 대한의료기공학회 2007 醫療氣功 Vol.10 No.1

자동비상제동 시스템의 안전성능평가

김태우,이경수,최인성,민경찬,Kim, Taewoo,Yi, Kyongsu,Choi, In Seong,Min, Kyong Chan 한국자동차안전학회 2015 자동차안전학회지 Vol.7 No.2

This paper presents a performance evaluation procedure for advanced emergency braking (AEB) system. To guarantee the performance of AEB system, AEB test scenario should contains various driving conditions which can be occurred in real driving condition. Also, performances of each elements of AEB system, such as sensor, decision, human machine interface (HMI) and control, should be evaluated in various situations. For this, driving conditions, road types, environment, and elements of AEB system were introduced. Test scenario has been designed to represent the real driving condition and to evaluate the safety performance of AEB system in various situations. To confirm that the proposed AEB test scenario is realistic and physically meaningful, vehicle test have been conducted in two cases of proposed AEB test scenario: subject vehicle cut-out scenario and narrow street turn left scenario.

태양광-수소생산을 위한 BVO 광전극 기반 광전기화학전지의 대면적 연구 개발 현황

김태우,Kim, Tae-U 한국전기전자재료학회 2021 전기전자재료 Vol.34 No.6

육미지황탕가미방(六味地黃湯加味方)이 C57BL/6JHam-ob/ob mice의 비만(肥滿) 및 제2형 당뇨병(糖尿病)에 미치는 영향(影響)

김태우,강석봉,Kim, Taewoo,Kang, Seok Bong 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.2

Objectives : The object of this study was to observe the pharmacological effects of Yookmijihwang-tang-gamibang (Liuweidihuang-tang-jiaweifang, YGB) consisted of 17 types of herbs has been traditionally used in Korean medicine for treating various disorders in clinics, aqueous extracts on the genetically obese and type II diabetic C57BL/6JHam-ob/ob(ob/ob) mice. Methods :Three different dosages of YGB were orally administered, once a day for 28 days to ob/ob mice with ob/ob control and C57BL/6JJms normoglycemic intact mice. Four weeks after treatments of YGB: the changes on the body weight, food consumption, blood glucose levels, leptin and adiponectin contents were observed for monitoring the antiobese and antidiabetic effects of YGB. The effects were compared to those of CLA(conjugated linoleic acid) which improve type II diabetes and inhibit related obesity. Results : After end of 28 days of continuous treatments, ob/ob control showed increases of adipocyte hypertrophy, vasodilated atrophic glomerulus which were detected with marked hyperplasia of pancreatic islets, insulin and glucagon producing cells. These obese and related type II diabetes induced in ob/ob mice were markedly and significantly inhibited by 28 days of continuous treatment of three dosages of YGB. The YGB 50mg/kg showed similar favorable effects on the diabetes and related diabetic complications as compared with CLA 750mg/kg in ob/ob mice of the present study. Conclusions : The results obtained in this study suggest that over 25mg/kg of YGB extracts favorably retarded the obese and type II diabetes in genetically obese and type II diabetic ob/ob mice.

주변도로 분포가 학교건축물의 소음실태에 미치는 영향에 관한 연구

김태우,이강국,홍원화,Kim, Tae-Woo,Lee, Kang-Guk,Hong, Won-Hwa 한국교육시설학회 2011 敎育施設 Vol.18 No.4

This study investigates interior and exterior environments and arrangement forms of school buildings from elementary to high school which were recently built in Daegu since the 7th Educational Curriculum. In addition, this study analyses how outside noise affects inside environment of schools. This study found that the noise level criteria of school facilities(such as exterior learning sites and outer walls of school buildings) adjacent to street of one side satisfiedly corresponded to the level and criteria, 65dB(A), of school health law. However, the noise level and vibration criteria of school facilities adjacent to street of two sides did not satisfiedly corresponded to the level or criteria, 65dB(A). In case of classrooms, the inner noise of educational facilities with windows closed and also close to both the street of one side and of two sides satisfiedly corresponded to the level or criteria. 55dB(A), of school health law. In spring and tall, the windows are more open them usual for ventilation, and leaving windows open can lead to noise from outside. Thus, it is urgent to prepare for this situation.

Web 2.0 기반 국가기준점 어플리케이션 설계

김태우,김정욱,서용철,Kim, Tae-Woo,Kim, Jyung-Wuk,Suh, Yong-Cheol 한국공간정보시스템학회 2009 한국공간정보시스템학회 논문지 Vol.11 No.3

삼각점, 수준점 등 국내에서 위치를 결정하는데 기본이 되는 모든 기준점은 공간정보의 기준이자 기반이 되기 때문에, 합리적이고 효율적이며, 지속적으로 관리되어야 한다. 한편 IT 및 통신기술이 발달함에 따라 공간정보를 다루는 모든 기술이 함께 발전하고 있으며, 모바일 디바이스의 대중화와 웹 2.0 환경이 구축됨에 따라 기준점의 관리를 실시간으로 가능케 하는 기술이 요구되고 있다. 이는 사용자의 편의를 보장하고 과학적이며 체계적인 기준점 관리를 도모한다. 본 연구에서는 웹 2.0 환경 하에서 Ajax 기반의 웹 어플리케이션을 설계하여 실시간으로 갱신되는 기준점 정보의 원활한 소통을 지원하고, 영상정보를 함께 제공함으로써 사용자 접근성을 강화하는 기술을 개발하였다. As a surveying standard of a nation, the Korean geodetic control points such as triangulation points and benchmarks require continuous and effective management toward a spatially enabled society. As the developments of information technologies, use of mobile devices like PDA (personal digital assistant) has been increasing and the Web 2.0 environment became an important part of GIS. In this context, the management systems for national geodetic control points should be upgraded in accordance with the current trend, particularly for more interactive user-interface on the Web 2.0. This paper describes the framework design and prototype implementation of a Web 2.0 GIS application for the management of Korean geodetic control points. Our system enables a real-time update of the control point information and provides aerial photos on vector maps using Ajax(Asynchronous JavaScript and XML) techniques.

다중 전달 경로 채널에서의 수중 디지털 통신을 위한 선 처리 기법

김태우,황아롬,성우제,임용곤,Kim, Tae-Woo,Hwang, A-Rom,Seong, Woo-Jae,Lim, Young-Kon 한국음향학회 2008 韓國音響學會誌 Vol.27 No.3

Signals in an underwater channel get distorted by multipath propagation. In this paper, pre-coding method is suggested which helps comprehending the signals with minimum equalization. The signals are transformed based on the knowledge of the impulse response of the channel. Proposed pre-coding method is tested by simulations based on the ray theory and through water tank experiments. In weak multipath environment, in case of an SNR of about 20 dB, BER is $10^{-3}{\sim}10^{-4}$, while in strong multipath environment, similar BER is achieved with SNR of about 30 dB. In order for the pre-coding method to be used for underwater vehicles, channel prediction method utilizing the waveguide invariant is suggested and tested. 수중 채널에서의 음파를 사용한 신호는 다중 전달 경로 현상에 의해 왜곡된다. 본 논문에서는 채널의 임펄스 응답을 바탕으로 신호를 미리 왜곡시켜 수신기에 도착했을 때 최소한의 등화만으로 신호를 받을 수 있는 선 처리 기법을 제안하였다. 그리고 음선 이론 기반 시뮬레이션과 수조 실험을 통해 제안한 기법의 성능을 검토하였다. 다중 전달 경로 현상이 약할 경우, SNR이 약 20 dB 일 때 BER이 $10^{-3}{\sim}10^{-4}$의 성능을 보였고 다중 전달 경로 현상이 심한 환경에서 SNR이 약 30 dB 일때 유사한 BER 성능을 보였다. 제안한 기법을 수중 운동체의 통신에 사용가능하도록 송수신기의 위치 변화에 따른 채널예측 기법을 제안하였고 그 성능을 검토하였다.