http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
T-2 toxin을 투여한 닭에서 Comet assay 방법을 이용한 DNA 손상 평가와 독성
하대식(Dae Sik Hah),허정호(Jung Ho Heo),이국천(Kuk Cheon Lee),조명희(Myung Heui Cho),김국헌(Kuk Hun Kim),김충희(Chung Hui Kim),류재두(Jae Du Lue),이승환(Seung Hwan Lee),김곤섭(Gon Sup Kim),김의경(Eui Gyung Kim),김종수(Jong Shu Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.2
This study was designed to evaluate the possible DNA damaging effects of T-2 toxin using an alkaline single cell gel electrophoresis (SCGE) comet assay and also to investigate toxic effects in chickens. A total of 20 chickens were used in these experiments. Graded concentrations of dietary T-2 toxin (0, 4, 8, and 16 ㎍/g of diet) were given to groups of 5 broiler chickens. In comet assay, The DNA damage was analysed by the tail extent moment (TEM) and tail length (TL), which were used as markers of DNA strand breaks in SCGE. A significant dose-dependent increase in the extent of DNA migration as well as in the percentage of cells with tails was observed after treatment with T-2 toxin (P<0.05). Treatment with the low T-2 toxin (4 μ/g of diet) induced a relatively low level of DNA damage in comparison with the high T-2 toxin (16 μ/g of diet) group. The growth rate was significantly reduced by concentrations of 8, and 16 μ/g of diet (P < 0.05). The feed conversion ratio were significantly affected by any concentrations (P < 0.05). The relative weight of the spleen, and lung was decreased by the growth inhibitory concentrations. The bursa of Fabricius, thymus, and kidney were decreased in relative weight by concentrations of 16 μ/g of diet. The relative weight of the liver and heart were unaffected. The hemoglobin (Hb), hematocrit (HCT), and mean corpuscular hemoglobin (MCH) were decreased at concentration of 16 μ/g of diet. As compared with control chickens, there was no marked change in serum components except uric acid in T-2 treated chickens. All lymphoid tissues retained atrophic and lymphoid cell depletion throughout the three weeks trial.
경남지역 야생들쥐에서 Orientia tsutsugamushi에 대한 항체 조사 및 PCR에 의한 검색
하대식,김영훈,박정웅,박재갑,김충희,류재두,정명호,허정호,서종립,조명희,이국천,김곤섭,김의경,김종수,Hah, Dae-Sik,Kim, Young-Hoon,Park, Jung-Ung,Park, Jae-Kap,Kim, Chung-Hui,Ryu, Jae-Doo,Jong, Myung-Ho,Heo, Jung-Ho,Shu, Jong-Lip,Cho, Myung-Heui 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.2
As a part of epidemiologic investigation of tsutsugamushi disease, the wild rodents which were captured in Gyeongnam area were diagnosed with indirect immunofluorescent antibody assay (IFA) and Polymerase Chain Reaction (PCR) to find if they have an antibody against Orientia tsutsugamushi. The conclusion was drawn as followings. (1) The captured 58 wild rodents showed that the subspecies distribution of Apodemus agrarius was 86.2%, Microtus fortis was 8.6% and Crocidura lasiura was 5.2%. (2) The antibody positive rate of O. tsutsugamushi Gilliam, Karp, Karto and Boryong by IFA method was 32.0% in Apodemus agrarius among 50 wild rodents and 40.0% in Microtus fortis among 5 wild rodents, respectively. It was negative in the case of all the 3 Crocidura lasiura. (3) The antibody titers on Apodemus agrarius, Microtus fortis and Crocidura lasiura against Gilliam, Karp, Karto and Boryong were measured between 1:20 and 1:640. The antibody titer against each antigen was in the order Boryong>Gilliam>Karp. (4) O. tsutsugamushi was detected from the blood, spleen and kidney from the artificially infected mice by IFA and PCR. IFA showed the positive response between 3 and 18 days after inoculation. On the other hand, positive response was found from all the samples by PCR. (5) From PCR of the genomic DNA extracted from the blood, spleen and kidney samples of the captured wild rodents, Boryong-specific amplification product with size of 210 bp, which is particular in Boryong, was detected from spleen and kidney samples, but not detected in the blood. (6) Boryong-specific amplification product was detected from spleen and kidney samples which were obtained at 3, 6, 12, 18 and 24 days after the infection with Boryong. But, it wasn't detected from the uninfected samples. (7) From PCR of spleen and kidney samples of the captured wild rodents, it was found that positive rate of O. tsutsugamushi in Apodemus agrarius and Microtus fortis were 25.0% (4/16) and 20.0% (1/5), respectively. From the above results, it can be concluded that Apodemus agrarius resided in Gyeongnam area carried O. tsutsugamushi and PCR method might be a simple, precise, rapid and useful diagnostic tool than IFA for the diagnosis of O. tsutsugamushi.
경남 남부지방에서 사육 중인 송아지의 설사병에 관한 역학적 연구
허정호 ( Jung Ho Heo ),조명희 ( Myung Heui Cho ),이국천 ( Kuk Cheon Lee ),박미남 ( Mi Nam Park ),조은정 ( Eun Jeong Cho ),최만수 ( Man Su Choi ),김충희 ( Chung Hui Kim ),강정부 ( Joung Boo Kang ),김의경 ( Eui Kyung Kim ),김종수 ( 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3
The present study was conducted to investigate the epidemiological characteristics such as isolation rate and antimicrobial drug susceptibility of etiological agents. The specimens (stool or intestine) were collected from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006. The isolation rate of Salmonella spp was higher in summer (8.4%) than in winter (4.8%) and the average was 7.2% (23/319 head). Some of Salmonella spp isolated were resistant to penicillin, oxytetracycline, tetracycline, and cephalexin (>90%), but some of them were suscep-tible to norfloxacin, ciprofloxacin, sulfamethoxazole/trimethoprim and amikacin(>30%). There was no statistical difference in the isolation rate of Eimeria spp between summer(48.9%) and winter(42.3%). For the evaluation of infection level of Eimeria spp oocyst per gram of feces(OPG) was examined, and severe, moderate and light infection level were 11.9%, 12.5% and 22.3%, respectively. In the isolation rates of Eimeria spp the calves under 19 days was lowerthan those over 60 days, but there was not different among herd size.
경남 남부지방에서 송아지설사병 원인체 바이러스 검출 조사
허정호 ( Jung Ho Heo ),조명희 ( Myung Heui Cho ),이국천 ( Kuk Cheon Lee ),박미남 ( Mi Nam Park ),조은정 ( Eun Jeong Cho ),최만수 ( Man Su Choi ),김충희 ( Chung Hui Kim ),강정부 ( Joung Boo Kang ),김의경 ( Eui Kyung Kim ),김종수 ( 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3
Among calves` diseases, digestive diseases most frequently occur in Korea, and especially viral diarrhea is one of the most important diseases. This study was conducted to get some basic information for the control of the viral diarrhea in calves. The samples were obtained from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006. Viral detection was examined by polymerase chain reaction (PCR). Etiological viruses were detected from 171(53.6%) of 319 calves, and bovine rotavirus (BRV) were 130 (40.8%) and bovine viral diarrhea virus (BVDV) 41 (12.9%), and no coronavirus was confirmed. Statistical difference was found in BRV detection between summer (32.6%) and winter (57.7%). However there was no seasonal difference in BVDV. In detection rate of the calves under 19days, BRV was highest (55.1%), but BVDV lowest (5.1%). No big difference was in rate among herd size. However, BRV was lowest (26.8%) in the group over 51 heads, but BVDV was highest (19.5%) in the same.
항구포자충제제의 사용 여부에 따른 구포자충 감염실태 조사
허정호 ( Jung Ho Heo ),정명호 ( Myung Ho Jung ),김국헌 ( Kuk Hun Kim ),조명희 ( Myung Heui Cho ),이국천 ( Kuk Cheon Lee ),서종립 ( Jong Lip Seo ),김충희 ( Chung Hui Kim ),하대식 ( Dae Sik Hah ),류재두 ( Jae Doo Ryu ),김곤섭 ( Gon 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.3
The present study was conducted to investigate the prevalence of Eimeria infection according to anti-coccidiosis drugs treatment in 1,300 slaughtered chickens from 130 farms in Gyeongnam, Jeonnam, Jeonbuk, Gyeongbuk and Chungnam during the period from October 2002 to August 2003. The prevalence of Eimeria infection by drug administration for treatment and prevention or non-administration was shown 71.4%, 82.6% and 72.3%, respectively, and so infection rate of chicken farms was similar independent on drug administration or not. The prevalence of Oocysts infection level of chicken farms by administrated drug for treatment was shown lower (25.3%) than for prevention (35.4%) and non-treatment (36.2%). The prevalence of Oocysts infection level by drug administration under 2 days for treatment was shown higher infection rate (32.2%) than over 3 days (20%) and also under 2 days for prevention has similar tendency of that of treatment and so, it was conclusion that administration of drug over 3 days has the effect for treatment and prevention of Eimeria infection. The prevalence of Oocysts infection level by age for prevention has various level from 48.8% to 22.9% but on 22~25 days was shown the most higher ratio (48.8%) and on 19∼21 days was shown the lowest rate (22.9%) among the all age groups.
조각자(주엽) 나무의 생리활성물질 Gleditschia이 비육돈육의 이화학적 성상에 미치는 영향
서종립,허정호,정명호,조명희,이국천,김국헌,하대식,류재두,김충희,김곤섭,김의경,김종수,Seo, Jong-Lip,Heo, Jung-Ho,Jung, Myung-Ho,Cho, Myung-Heui,Lee, Kuk-Cheon,Kim, Kuk-Hun,Hah, Dae-Shik,Ryu, Jae-Doo,Kim, Chung-Hui,Kim, Gon-Sup,Kim, Eui-Gy 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4
The effects of dietary Gleditschia on fatty acid composition, lipid oxidation, and pork quality were investigated. Pigs (n=40) were fed a diet containing 0, 0.1, 0.2, 0.4, and 1% Gleditschia for 14 weeks and slaughtered at 110 kg average. The longissimus throracis et lumborum muscle was collected at 24 hr postmortem. Pork loin chops (3 cm thick) were packaged aerobically and stored at $4^{\circ}C$ for 7 days. Samples were analyzed for fatty acid composition, ultimate pH, thiobarbituric acid-reactive substance (TBARS), color ($L^*$, $a^*$, $b^*$), drip loss and water-holding capacity. There was no significant difference of moisture and crude fat percent in between treatment and control group, and the color stability of pork loin better in all treat groups was more improved during cold storage. The change of pH was significantly increased (p<0.05) in 0.2, 0.4, 1% treatment groups compare to the control group. Water-holding capacity of pig loins was significantly higher (p<0.05) in all treat groups than in control group; 0.2% treat group was the highest in the water holding capacity followed by 0.4, 1, and 0.1% treat groups. Less drip loss of pig loin was observed with samples from Gleditschia-fed pigs except 1% treat group. Unsaturated fatty acid were tend to be decreased and saturated fatty acid were tend to be increased in Gleditschia-treated group. Thiobarbituric acid-reactive substance value of control was significantly higher than that of the Gleditschia-fed group (p<0.05).