수세미외 섬유의 펄프화에 관한 연구 - 섬유의 구조와 펄프화별 특징 분석

김종규,노재성,이종신 ( Jong Gyu Kim,Jae Seong Rho,Jong Shin Lee ) 한국공업화학회 1997 공업화학 Vol.8 No.6

수세미외 그물 섬유를 크라프트법, 알카리 아황산법, 아황산법,알카리 과산화 수소법과 소오다법으로 펄프화하고, 안트라퀴논의 첨가와 미첨가 조건으로 구분하여 증해하였다. 이들의 고해와 미고해분에 대하여 주사 전자현미경(SEM), 섬유 품질 분석기(FQA), 섬유길이 분류기(Clark 4-Screen Classifier), 화상 분석기(Image Analyzer)를 이용하여 특성과 섬유의 구조를 비교 분석하였다. 그 결과는 다음과 같다. 1) 수세미외 섬유의 증해후 해섬 가능 기준의 Pulping 조건은 KP계(160℃, 2시간), ASP계(155℃, 4시간), PAP계(160℃, 1시간)에서 Kappa값이 각각 12, 25, 10 수준으로 비교적 낮은 Total Alkali(약 20%) 조건에서 적정 증해가 가능했다. 2) 각 펄프화별 펄프의 총 증해수율은 KP 50∼55, ASP계 60∼70, PAP계 45∼50%로 SP계의 수율은 매우 높고, KP나 PAP는 일반 비목재자나 목재와 비슷한 수준을 나타냈다. 3) NaOH의 투입량의 증가는 해섬능을 촉진하고, 섬유길이, Curl, Kink Index 등에서 품질의 형태 변화를 보였다. 4) 수세미외 섬유의 펄프화 공정에서 AQ첨가는 탈리그닌 촉진으로 해섬능이 현격하게 향상되고 섬유의 산화 분해를 방지하며, 고해 속도 상승과 피브릴화를 촉진하였다. 5) ASP계는 KP나 PAP보다 Bulk density가 높고, 섬유간 결합이 치밀하고, 섬유의 세포 손상이 감소되었다. 6) 수세미외 섬유는 “C” Stain에 의한 정색 반응으로 청색 또는 청회색의 맑고 투명한 새포벽을 갖는 정색 특성을 나타냈다. Studies were carried out on the preparation of several kinds of pulps from Sponge gourd fiber by KP, ASP, SP, PAP, AP and addition of AQ pulping process. These unbeaten and beaten pulping fibers were observed their characteristics and fiber structure by SEM, FQA, Image analyzer and Micro projector. The results were summarized as follows ; 1) The cooking condition which is the possible defibrilation of Shives are KP base(160℃, 2hr.), ASP base(155℃, 4hr.), PAP base(160℃, lhr.). From the results, the kappa no. had the range of 12, 25, 10 each other. 2) The pulp yields of sponge gourd fiber obtained the range of KP 50∼55%, ASP & 60∼70% and PAP 45∼50%. SP base have the highest and contents of KP & PAP base are much the same as woods. 3) Increasing amount of NaOH on Pulping was accelerated the defibrilation of Shives and was changed a morphology of pulping fiber quality such as fiber length, curl and kink index. 4) Addition of AQ on pulping process of sponge gourd fiber had a affect to raise the rate of delignification while protecting cellullosic components against degradation, especially defibrilation was very excellent, beated pulp much more easily and increased the fibrilation. 5) ASP system have higher bulk density, fiber bonding and protecting cellullosic components against degradation than KP or PAP. 6) The color reactions of the C stain solution showed blue or blue-gray with clean and transparency thin cell wall.

DHPLC의 화학적 특성을 이용한 질병 유전자의 분석 시스템 개발

김종규,남윤형,박상범,이재식,강원,Kim, Jong-Gyu,Nam, Yun-Hyeong,Park, Sang-Beom,Lee, Jae-Sik,Gang, Won 대한화학회 2006 대한화학회지 Vol.50 No.2

종양억제유전자(tumor-suppressor gene)는 유방암종과 관련하여 높은 돌연변이 비율로 나타나고 있는 것으로 보고되어지고 있다. p53 유전자는 20kb의 크기를 갖는 유전자로써 인간 염색체의 17p13.1에 위치하고 있다. 본 실험에서는 유방암으로 진단 받고 수술한 환자의 조직 100개와 환자와 전혀 상관없는 정상 조직 103개를 대상으로 DNA를 추출하고 PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatography) 방법으로 단일 염기 다형성을 검출하였다. 또한 컬럼의 충진물질과 DNA의 결합에 의한 분리능을 확인하기 위해 충진물이 다른 컬럼의 단일 염기 다형성을 실험하였다. 그 결과 100개의 유방암 조직 중 exon 5에서 11개(11%)의 C/A, C/G genotype을, exon 8에서 42개(42%) T del genotype을 확인하였다. 103개의 정상 조직에서 exon 5에서 2개(2.9%), exon 8에서 9개(8.7%)의 polymorphism을 확인하였다. 컬럼의 분리능 실험에서는 PS-DVB(poly styrene - divinylbenzene)으로 충진된 컬럼이 C18으로 충진된 컬럼보다 더 좋은 분리능을 보였다. In this study we extracted DNA from 100 tissues of breast cancer patients and 103 normals. Then we confirmed single-nucleotide polymorphism(SNP) using PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatogrphy).Also, we studied SNP of samples using several columns to identify relation between packing materials of column and resolution.As a result, we identified 4 C/A, C/G genotypes(4%) in exon 5 and 37 T del genotypes(37%) in exon 8 among 100 breast cancer tissues and 2 in exon 5, 9 in exon 8 among 103 normal samples.In resolution test, we confirmed that PS-DVB(poly styrene-divinylbenzen) column is more efficient than C18 column.

일반인과 식품취급자의 손 위생관리에 관한 비교

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.3

Objectives: This study was performed to investigate hygienic behavior of food handlers and general population focusing on awareness of hand-washing and the microbial load of their hands. Methods: A questionnaire survey and microbiological analysis were carried out for sixty-four people each. Samples for microbiological analysis were collected through the glove-juice method from the hands, and were analyzed for the presence of aerobic plate counts, total coliform, fecal coliform, Escherichia coli, Staphylococcus aureus, and Salmonella spp. according to the Food Code of Korea. Results: In the survey, significant differences between the food handlers and general population (p < 0.01) were found in hand-washing frequency, duration, use of hand-washing agents, washing parts of hands, hand-drying method, and method of turning off water. In eight different situations among the ten particular situations in their daily life, more food handlers responded to wash their hands than general population (p < 0.05). Bacterial load on hands with general population was consistently higher than with food handlers (p < 0.05), however, percentages of positive hands of S. aureus and Salmonella spp. were not. Conclusions: Poor hand hygiene practices were indicated by the positive results for E. coli, S. aureus, and Salmonella spp. on the hands of some respondents in both groups. This study reveals that there is the need for programs or campaigns to increase hand-washing practices of both groups.

화학물질 관리에 대한 이해 63 - 사업장에서 생식기능 생식능력 또는 태아 발생 발육에 유해한 영향을 줄 수 있는 주요물질

김종규,Kim, Jong-Gyu 대한산업보건협회 2016 산업보건 Vol.342 No.-

맨손과 장갑 낀 손의 미생물 오염도 비교

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2011 한국환경보건학회지 Vol.37 No.4

Objectives: There has been a great amount of controversy in the food industry over the effectiveness of using gloves. The objectives of this study were to examine the microbial contamination of food handlers' hands and determine if using gloves could ensure safe handling of foods. Methods: Samples were collected through the glove-juice method from the bare and gloved hands of food handlers at work and were analyzed for the presence of aerobic plate counts, total coliform, fecal coliform, Escherichiacoli, Staphylococcus aureus, and Salmonella spp. Microbiological testing was conducted according to the Food Code of Korea. Results: Microbial contamination was consistently higher with bare hands than with gloved hands, although the microbial loads on both hands increased over time. Under certain conditions, there were significant differences between the bacterial loads on bare and on gloved hands (p<0.05). E. coli, S. aureus, and Salmonella spp. were still alive on both bare and gloved hands at the time the food handlers finished their work. Conclusions: Wearing gloves was associated with a marked reduction of bacterial contamination of the hands. However, the practice of continuously wearing gloves during food handling increases the potential for cross-contamination of bacteria. The findings of this study emphasize the need for a rational use of gloves, and strict adherence to hand hygiene compliance among food handlers.

개에서 실험적으로 유발한 복수증의 초음파 진단

김종규,Kim Jong-gyu 한국임상수의학회 1995 한국임상수의학회지 Vol.12 No.1

In this study, ultrasonographic evaluations were made after experimentally induced ascites in dogs. Ultrasonographic evaluation allowed to identify the earliest fluid accumulation between the spleen and the left kidney after the administration of 0.9% normal saline at 8 ml/kg into the abdominal cavity in dogs. Ultrasonographic finding were observed in order between the spleen and the left kidney, the liver, the pelvic cavity. It is considered that the most adequate pare f3r the early detection of ascites is between the spleen and the left kidney, and the least amount of detection fluid is 8 ml/kg in dogs.

초등학생의 식품위생안전에 대한 지식, 태도 및 실천에 관한 연구

김종규,김중순,Kim, Jong-Gyu,Kim, Joong-Soon 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.3

Objectives: The purpose of this study was to evaluate food hygiene and safety knowledge, attitudes, and practices (KAP) among the elementary school children, and furthermore, to explain the interrelations among these three variables. A second purpose was to compare gender differences of KAP. Methods: A self-administered, structured questionnaire was designed and completed by 376 elementary school children. A five-point Likert scale with twenty questions was used to gather information. Data was analyzed by frequency analysis, Pearson's correlation and analysis of variance or Student's t-test. Results: Children generally had good knowledge of food hygiene and safety, and knowledge of children was superior to the attitudes and also to their practices (p < 0.05). The attitude score was mediated between knowledge and practice scores. Significant differences (p < 0.05) were found between boys and girls in an item of knowledge, three items of attitudes, and six items of practices. A significant positive correlations were observed between knowledge and practices (${\gamma}$ = 0.70, p < 0.001), and between attitudes and practices (${\gamma}$ = 0.45, p < 0.001), as well as between attitudes and practices (${\gamma}$ = 0.51, p < 0.001), revealing that increased knowledge and even attitudes toward food hygiene safety could result in positive change in practices or behaviors. Conclusions: The results of this study indicate that despite good knowledge and attitudes of children, their practices toward food hygiene and safety are not acceptable. Gender differences in practices were highlighted. In order to create effective educational interventions on the food hygiene and safety of children, a better understanding of their awareness and the required related information is necessary.

반도체 발광 다이오드의 역사

김종규,Kim, Jong-Gyu,Schubert, E. Fred 한국광학회 2010 광학과 기술 Vol.14 No.3

발광 다이오드가 처음 만들어진 것은 100년 전으로 거슬러 올라간다. 이 글은 100년 전 발광 다이오드가 우연히 발견된 이후부터 현재 없어서는 안될 귀중한 고출력/고효율 대체 조명용 광원으로 인식되기까지의 역사를 돌아보고자 한다. 또한 앞으로 기대되는 발광 다이오드의 발전가능성과 응용성에 대해서도 다루고자 한다.

일부 음식점 주방의 부유세균 및 부유진균 조사연구

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.2

Objectives: The purpose of this study was to determine airborne bioaerosols such as bacteria and fungi in the kitchens of restaurants, and to assess the effects of thermal factors on the levels of the bioaerosols. Methods: Air samples were taken from kitchens of nine restaurants. An Anderson type air sampler was used for sampling and measurements. Petri dishes filled with a microbiological culture medium (trypticase soy agar for bacteria and Sabouraud dextrose agar for fungi) were used as the sampling surface. Results: The levels of bacterial aerosol measured were $10-10^3CFU/m^3$ and fungal aerosol $10-10^2CFU/m^3$, respectively The mean values of air temperature and relative humidity in the kitchens were $24.6^{\circ}C$ and 46.4%, respectively. Overall, the levels of bacterial aerosol varied by the restaurant type, and fungal aerosol by the business period (p < 0.05). The main effect of air temperature and interaction effect of air temperature and relative humidity onto the bacterial level were significant (p < 0.05), whereas the effects were not significant onto the fungal level. Conclusions: The results indicate a wide variation in the levels of bioaerosols among different kitchens. The observed differences in bioaerosol levels in the kitchens reflect different periods of use. The interactions of air temperature and relative humidity onto the bacterial level suggest that constant attention should be paid to avoid peaks of contamination during the summer season.

남자 대학생의 손 씻기 의식과 실천에 관한 연구

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Young,Kim, Joong-Soon 한국환경보건학회 2009 한국환경보건학회지 Vol.35 No.1

Hand-washing has been recognized as a critical factor in controlling infection and cross-contamination. This study was performed to investigate hygienic behavior of male university students focusing on awareness of hand-washing, their hand washing practices, and the difference between their awareness and practices. Both a self-administered questionnaire survey and a separate direct observation in restrooms were carried out at a university campus over a four week period. In the survey, 93.6% of the responded students claimed to wash their hands after using toilet. However, just 16.9% of the observed students actually did so, according to a separate and unnoticed observational study. Among the observed students who washed their hands, 25.0% of them used soap, only 5.0% of them washed four parts of their hands, and 15.0% of them washed their hands for more than 10 seconds. Paper towel was the most common hand drying method in the direct observation and also in the survey. Significant differences between the questionnaire survey and the direct observation (p<0.0l) were found in hand-washing practices, duration of hand-washing, using soap, washing parts of the hand, and hand-drying method after using the toilet. This study indicates that there is a remarkable difference between the male university students' awareness of hand-washing and their hand-washing practices. This study reveals that there is the need for programs or campaigns to increase hand-washing practices of male university students.