토양에서 분리된 Aeromonas hydrophila AM-28이 생산하는 단백질 가수분해효소의 특성

김인숙,김형권,이정기,배경숙,오태광,Kim, In-Sook,Kim, Hyung-Kwoun,Lee, Jung-Kee,Bae, Kyung-Sook,Oh, Tae-Kwang 한국미생물학회 1994 미생물학회지 Vol.32 No.4

토양으로부터 탈지대두박을 잘 분해하는 단백질 가수분해효소를 생산하는 미생물을 분리하여 형태적, 생화학적 특성과 세포벽 지방산조성을 조사한 결과, 분리된 세균은 Aeromonas hydrophila로 동정되었다. 분리된 세균은 glycerol을 탄소원으로, tryptone 및 $(NH_4)_2HPO_4$을 진소원으로, $CaCl_2$를 무기원으로 사용한 배지에서 효소를 가장 많이 생산하였으며, 생산된 효소의 반응최적 pH는 8.0이고, 반응최적온도는 $65^{\circ}C$였다. 이 효소는 $55^{\circ}C$ 이하의 온도에서, pH 7.0에서 13.0의 범위에서 비교적 안정한 것으로 나타났다. 또한 이 효소는 금속이온 $Cu^{2+},\;Hg^{2+}.\;Mn^{2+}$ 및 $Zn^{2+}$에 의해서 효소활성이 저해를 받고, 효소저해제 phenylmethylsulfonyl fluoride (PMSF)와 ethylenediaminetetraacetic acid (EDTA)에 의해서 효소활성이 강력히 저해되는 성질로부터 효소활성부위에 serine기와 금속이온을 갖고 있는 것으로 밝혀졌다. A bacterial strain NO. AM-28, showing proteolytic activity against defatted soybean was isolated from domestic soil. The isolated strain was identified as Aeromonas hydrophila by both the biochemical tests using API kit and the analysis of cellular fatty acid profile with MIDI system. The protease production from A. hydrophila AM-28 was highly enhanced when it was cultivated in the medium containing glycerol as a carbon source, tryptone or $(NH_4)_2HPO_4$ as a nitrogen source, and $CaCl_2$ as a mineral source. The optimal pH and temperature for the enzyme was 8.0 and $65^{\circ}C$, respectively. The enzyme was stable up to $55^{\circ}C$ and at pH values ranging from 7.0 to 13.0. The enzyme activity was inhibited by phenylmethylsulfonyl fluoride and EDTA, indicating that serine residue and metal ions be involved in enzyme activity.

사람의 골수 줄기 세포로부터의 골세포 분화 과정에서 BMP-2가 미치는 영향과 그에 따른 분화 유전자의 발현 비교 연구

김인숙,장옥련,조태형,이규백,박용두,노인섭,황순정,김명진,이종호,Kim, In-Sook,Zhang, Yu-Lian,Cho, Tae-Hyung,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Hwang, Soon-Jung,Kim, Myung-Jin,Lee, Jong-Ho 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

쥐의 골수로부터 추출한 줄기세포를 이용한 조골세포로의 분화 유도과정에서 나타난 문제점에 관한 분석 연구

김인숙,조태형,장옥련,이규백,박용두,노인섭,이종호,김명진,황순정,Kim, In-Sook,Cho, Tae-Hyung,Zhang, Yu-Lian,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Lee, Jong-Ho,Kim, Myung-Jin,Hwang, Soon-Jung 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1

This study was aimed to characterize osteogenic potential of rat bone marrow stromal cells (BMSC) isolated with standard flushing method and investigate the plasticity of transdifferentiation between osteoblastic and adipocytic lineage of cultured BMSC. Unlike aspiration method in human, rat bone marrow was extracted by means of irrigation with culture media that elevates the possibility of co-extraction of committed osteoprogenitor, or preosteoblast or other progenitor cells of several types present inside bone marrow. The cultured stromal cells showed high ALP activity which is representative marker of osteoblast without any treatment. Osteogenic inducers such as Dex and BMP-2 were examined for the evaluation of their effect on osteogenic and adipocytic differentiation of stromal cells, because they function as osteoinductive agent in stromal cells, but simultaneously induce adipogenic differentiation. Osteogenic differentiation was evaluated by measuring alkaline phosphatase activity or mRNA expression of osteoblast markers such as osteopontin, bone sialoprotein, collagen type I and CbfaI, and in vitro matrix mineralization by von Kossa staining. Oil red staining method was used to detect adipocyte and adipocytic marker, aP2 and $PPAR{\gamma}2$ expression was examined using RT-PCR. It can be supposed that irrigation procedure resulted in high portion of already differentiation-committed osteoprogenitor cell showing elevated ALP activity and strong mineralization only under the supplement of $100{\mu}M$ ascorbic 2-phosphate and 10mM ${\beta}$-glycerophosphate without any treatment of osteogenic inducers such as Dex and BMP-2. Dex and BMP-2 seemed to transdifferentiate osteoprogenitor cells having high ALP activity into adipocytes temporarily, but continuous treatment redifferentiated into osteoblast and developed in vitro matrix mineralization. This property must be considered either in tissue engineering for bone regeneration, or in research of characterization of osteogenic differentiation, with rat BMSC isolated by the standard irrigation method.

논문편 : 농촌여성 노동의 화폐적 가치평가를 위한 일 연구

김인숙,최은숙 ( In Sook Kim,Eun Sook Choi ) 대한가정학회 1991 Family and Environment Research Vol.29 No.2

논문편 : 가정관리능력 및 가정 생활만족도에 따른 농가유형 구분

김인숙,김진호 ( In Sook Kim,Jin Ho Kim ) 대한가정학회 1996 Human Ecology Research(HER) Vol.34 No.6

논문편 : 농가유형별로 본 농가소득 및 소비지출 구조분석 - '88 농가가계부를 중심으로 -

김인숙,최은숙 ( In Sook Kim,Eun Sook Choi ) 대한가정학회 1990 Family and Environment Research Vol.28 No.3

한국인의 성인 하악 치조골 결손 형태의 빈도 및 분포에 관한 연구

김인숙,감한평,김종관,Kim, In-Sook,Kim, Han-Pyong,Kim, Chong-Kwan 대한치주과학회 1983 Journal of Periodontal & Implant Science Vol.13 No.1

조직배양에 의한 위도상사화(Lycoris uydoensis M. Kim)의 효율적 기내번식

고정애 ( Jeong Ae Ko ),김인숙 ( In Sook Kim ),정공수 ( Gong Soo Jeong ),최정란 ( Jeong Ran Choi ) 전북대학교 농업과학기술연구소 2012 농업생명과학연구 Vol.43 No.2

This study was conducted to establish an effective in vitro mass propagation system using leaf and bulb scale explant sources of Lycoris uydoensis M. Kim. For the bulb scale culture, the bulbs treated at 5℃ for 60 days were dissected into three parts according to bulb height (upper, middle, and bottom), and allowed for a culture on the MS medium with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA, 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA, and 3.0 mg ? L-1 IBA plus 3.0 mg ? L-1 BA. Regardless of plant hormone treatments, the bottom part of the bulb including the disk proved to be most effective in forming the callus and bulblets. Callus was readily produced on the young leaf segment and bulb scale tissues, whereas, bulblets and shoots were formed only on bulb scale explants. Bulb scales with disk appeared to the best optimal explant for in vitro propagation. Two distinct pathways, either organogenesis through callus or direct bulblet formation, could be recognized in bulb scale culture. Bulblet formation and shoot organogenesis from the bulb scales with disk were effectively induced up to the 60%~100% on the MS medium supplemented with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA, 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA, and 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA within 30 days of culture. On MS media supplemented with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA or 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA were more effective than 0.5 mg ? L-1 NAA plus 1.0 mg ? L-1 BA for induced and maintained a large number of bulblets. Six percent of sucrose enhanced bulblet formation and expanded the bulblets. Especially, adding 6% of sucrose on MS media supplemented with 3.0 mg ? L-1 IBA와 3.0 mg ? L-1 BA was one or two times of bulblets expanted than on MS mediun with 0.1 mg ? L-1 2,4-D plus 1.0 mg ? L-1 BA plus 6% of sucrose. Plantlet regeneration was successfully achieved from bulb scale-derived callus, via shoot bud induction or direct bulblet formation.

차문화 융합 프로그램이 이용시설 노인의 의사소통 향상에 미치는 영향

김인숙,박선희,Kim, In Sook,Park, Sun Hee 한국사물인터넷학회 2020 한국사물인터넷학회 논문지 Vol.6 No.1

The purpose of this study was to examine the effect on improving communication by conducting a tea culture convergence program for elderly people using facilities. The subjects of this study consisted of a tea culture convergence program for 3 months, 60 minutes once a week for 7 people using the Elderly Day Care Center in the H-S S welfare center. Psychological and behavioral changes related to communication by sessions were compared by comparing elderly patients at each session of the Tea Culture Convergence Program.Tea culture convergence program was found to have a positive effect on behaviors and attitudes related to communication among the elderly in the facilities. As a result of analyzing pre- and post-action and attitudes, the Tea Culture Convergence Program brought positive changes in communication by acting as emotional and social support for the elderly. These findings suggest that the Tea Culture Convergence Program is a psychologically necessary program for the elderly in that it shows that the Tea Culture Convergence Program is an effective program that promotes communication among the elderly in the facilities. 본 연구는 이용시설노인들을 대상으로 차문화 융합 프로그램을 실시하여 의사소통 향상에 미치는 효과를 살펴보고자 하였다. 연구 대상은 H시 S복지관 내 노인주간보호센터를 이용하는 7명을 주 1회 60분씩 3개월 간 차문화 융합프로그램을 진행하였다. 차문화 융합 프로그램의 각 회기별 노인들이 차문화 융합 프로그램에 참여하는 연구대상자들에 대한 회기별 의사소통과 관련된 심리 및 행동 변화를 기록하여 비교하였다. 차문화 융합 프로그램이 이용시설 노인들의 의사소통과 관련된 행동 및 태도에 긍정적인 효과를 주는 것으로 나타났다. 사전과 사후의 행동 및 태도를 분석한 결과 차문화 융합 프로그램이 노인들에게 정서적, 사회적 지지의 역할을 함으로써 의사소통에 긍정적인 변화를 가져왔다. 이러한 연구 결과는 차문화 융합 프로그램이 이용시설 노인들의 의사소통을 증진시키는 효율적인 프로그램임을 보여 준다는 점에서 노인들에게 차문화 융합 프로그램은 심리사회적으로 꼭 필요한 프로그램이라는 점을 시사한다.

여성노인의 차(茶)문화치료 융합프로그램 경험 현상 연구: 주간보호센터 이용 노인대상으로

김인숙,박선희,Kim, In-Sook,Park, Sun-Hee 한국사물인터넷학회 2021 한국사물인터넷학회 논문지 Vol.7 No.1

The purpose of this study is to explore elderly women's experience of the convergent tea culture therapy program when they use day care centers, ultimately getting a better understanding of such experience. For the purpose, the study made profound interviews with 4 old women who experienced the convergent tea culture therapy program from its start to final session. That program was provided by H day care center located in P city. And then, this research analyzed data from those interviews by using the phenomenological methods. As a result of the analysis, the study could divided the surveyed female elders' experience of the above mentioned program largely into 4 categories, which are, 'getting away from daily routines', 'feeling as if I'm the queen when having tea', 'finding myself feeling younger with tea', and 'feeling the true sense of happiness with tea'. With this result taken into serious consideration, this study suggested that more attention and intervention be urgently needed in the field of senior welfare practices, especially leisure activity for elders which is currently in trouble due to the covid-19 crisis. 본 연구의 목적은 주간보호센터 이용 여성노인을 대상으로 차(茶)문화치료 융합프로그램 경험을 탐색하고 그 융합프로그램 경험을 이해하는 것을 목적으로 한다. 이를 위해 P시 H주간보호센터를 중심으로 차(茶)문화치료 융합프로그램 경험을 마지막 회기까지 참여 한 여성노인 4명을 대상으로 심층 면담을 수행하였으며 자료 분석은 Colaizzi의 현상학적 분석 방법으로 하였다. 분석결과 주간보호센터 이용 여성노인을 대상으로 차(茶)문화치료 융합프로그램 경험의 4개의 범주인 되돌림의 일상, 차와 함께 나는 대비마마, 차와 함께 젊은 그대, 차와 함께 행복의 나라로 분석하였다. 차(茶)문화치료 융합프로그램 경험에 대한 분석 결과를 토대로 노인복지실천 영역에서 코비드 19로 인한 노인 여가활동에 관심과 개입이 시급함을 제시하였다.