동결 건조된 리포솜의 안정화에 있어서 말토스의 영향

김윤아,한희동,신병철,Kim, Yun-A,Han, Hee-Dong,Shin, Byung-Cheol 대한화학회 2004 대한화학회지 Vol.48 No.6

약물 수송체로 사용되는 리포솜은 동결 건조를 통하여 분말화 시키는 방법이 사용되어지고 있다. 리포솜 안정제로서 말토스는 동결건조된 리포솜 분말의 안정성을 향상시키고 약물 봉입률에 영향을 주는 것으로 알려져 왔다. 동결건조 전과 후의 리포솜 입자의 크기를 측정하므로서 리포솜의 안정성을 평가하였고 또한, 약물 봉입률은 모델 약물인 칼세인을 사용하여 조건에 따른 봉입양을 측정하였다. 리포솜 제조 후에 말토스를 첨가한 리포솜은 수화하는 과정에서 말토스를 첨가하여 제조한 리포솜보다 훨씬 더 안정한 것으로 확인되었다. 말토스를 첨가하지 않은 리포솜은 시간이 지남에 따라 입자의 크기가 커지는 반면, 리포솜 제조 후에 말토스를 첨가한 리포솜은 $4{\sim}37^{\circ}C$에서 30일 동안 안정하다. 또한, 말토스/지질의 물농도 비가 3과 6일 때 상대적으로 가장 높은 안정성을 보였다. Liposome powders were prepared by a freeze-drying method for the application to the field of drug carrier. The effect of maltose as a liposome stabilizer was studied on the stability and the drug-loading efficiency of the freeze-dried liposome powders. The particle size of liposomes before and after freeze-drying was determined to evaluate the liposome stability. The drug-loading efficiency was measured by Fluorescence spectrophotometer using calcein as a model drug. When maltose was added after the preparation of the liposomes, the liposomes was stable, compared to the case of maltose addition at the hydration procedure. By the addition of maltose, the liposome was stable for 30 days at $4{\sim}37^{\circ}C$, while the particle size of the liposome without maltose increased with time. The liposome showed relatively high stability when the maltose/lipids molar ratio was 3 and 6.

2005년 한국에서 시판된 먹는 샘물의 미생물 오염

김윤아,이도경,유경미,강병용,하남주,Kim Yun-A,Lee Do-Kyung,Yu Kyoung-Mi,Kang Byung-Yong,Ha Nam-Joo 환경독성보건학회 2006 환경독성보건학회지 Vol.21 No.3

In recent years, there has been an increase in consumer demand for bottled waters. There is a perception that consumption of natural mineral water represents a healthy life style and that these products are relatively safe. In this study, the microbiological quality of 39 samples of bottled water, purchased from retail store in Korea, was investigated during the 2005. Applying pour plate method, the 1 mL of water samples were analyzed for the presence and enumeration of total general bacteria and Pseudomonas spp.. Nineteen samples representing 9 brands of bottled water contained general bacteria ($1.54{\times}10^2$ CFU/mL). In addition four samples contained Pseudomonas spp. and Camamonas acidovorans. The susceptibility of the strains tested against 25 antimicrobial agents, Pseudomonas fluorescens were resistant to Lincomycin, Amoxacilin/Clavulanic acid and Cefazolin (> $100{\mu}g/mL$). Also Comamonas acidovorans were intermediate to Cephalothin and resistant to Cefoperazone.

하시모토갑상선염에 동반된 쇼그렌증후군

김윤아 ( Yun A Kim ),이신석 ( Shin Seok Lee ),정해권 ( Ha Gwon Juhng ) 대한류마티스학회 2002 대한류마티스학회지 Vol.9 No.1

Sjogren`s syndrome (SS) is a chronic, slowly progressive autoimmune disease characterized by lymphocytic infiltration of the exocrine glands leading to dry mouth and dry eyes accompanied by a variety of autoimmune phenomena. Despite the obvious difference in the affected organ, patients with SS and those with Hashimoto`s thyroiditis share several histologic and genetic features. The immunopathologic findings of Hashimoto`s thyroiditis and Sjogren`s sialadenitis show similarities such as infiltration by activated T lymphocytes and inappropriate expression of HLA class II molecules by the epithelial cells. It has been reported that up to 50% of patients with SS develop autoimmune thyroid disease in the clinical course, and patients with Hashimoto`s thyroiditis have symptoms related to SS frequently. In Korea, the ocurrence of SS in association with Hashimoto`s thyroiditis has not been reported so far, although it is considered to coexist occasionally. We report a case of primary SS in a patient with Hashimoto`s thyroiditis who had been treated with levothyroxine.

전신성 홍반성 루푸스에 동반된 다발성 골수종

이수정 ( Soo Jung Lee ),이신석 ( Shin Seok Lee ),김윤아 ( Yun A Kim ),박미정 ( Mi Jeong Park ),이제중 ( Je Jung Lee ),김형준 ( Hyeoung Joon Kim ) 대한류마티스학회 2002 대한류마티스학회지 Vol.9 No.4

It has been suggested that there is an increased risk of cancer in patients with systemic lupus erythematosus (SLE), even when cytotoxic drugs are not used. The coincidence of plasma cell tumor and lupus nephritis has been documented in animal models, but multiple myeloma is rarely associated with SLE in humans. No case of multiple myeloma in SLE has been reported in Korea. We report a case of multiple myeloma in a patient with SLE. The patient was a 49-year-old woman, who had polyarthralgia for 2 years. Progressive, painful swelling in both elbow joints developed 3 months before admission. The diagnosis of SLE was based on the findings of nondeforming arthritis, neutropenia, and positive ANA and anti-Sm antibody. Laboratory studies showed hypergammaglobulinemia and an elevated serum immunoglobulin (Ig) G level. Serum protein electrophoresis disclosed an M spike and serum immunoelectrophoresis showed a monoclonal IgG lambda type. Bone marrow aspiration revealed 15% plasma cells. Therefore, we diagnosed this patient as having multiple myeloma and SLE simultaneously.

Android 플랫폼 기반 플래시 메모리 관리 기법에 대한 성능 평가

김윤아 ( Yun-a Kim ),오기환 ( Gi-hwan Oh ),김강년 ( Kang-nyeon Kim ),강운학 ( Woon-hak Kang ),이상원 ( Sang-won Lee ) 한국정보처리학회 2011 한국정보처리학회 학술대회논문집 Vol.18 No.1

스마트폰에서 낸드 플래시 메모리가 저장 장치로 사용됨에 따라 다양한 플래시 파일 시스템과 플래시 변환 계층들이 제시되었다. 플래시 메모리는 덮어 쓰기가 불가능하기 때문에 이들 기법들은 기본적으로 로그 기반 구조를 취하고 있지만 가비지 수집, 데이터 배치 정책의 설계에 따라 성능과 수명 관리 측면에서 많은 차이를 보인다. 본 논문은 플래시 메모리 관리 기법들의 다양한 설계가 성능에 미치는 영향을 알아보고 종합적으로 비교 해보기 위해 대표적인 스마트폰 플랫폼인 안드로이드상에서 시뮬레이션 기반의 성능 평가를 수행 한다. 또한 각 기법들의 설계가 성능에 미치는 영향을 분석 한다.

일란성 쌍태 중 일측 태아의 인어체기형 (Sirenomelia)

정상근 ( Jeong Sang Geun ),김현철 ( Kim Hyeon Cheol ),김유신 ( Kim Yu Sin ),김윤아 ( Kim Yun A ),김조영 ( Kim Jo Yeong ),김지연 ( Kim Ji Yeon ),신승주 ( Sin Seung Ju ),김낙근 ( Kim Nag Geun ),강혜윤 ( Kang Hye Yun ),박정선 ( Park 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.4

Sirenomelia is a lethal congenital malformation characterized by single or fused lower limbs associated with other severe genitourinary and lower gastrointestinal tract anomalies. Associated anomalies include malformation of vertebrae and pelvis, oligohyd

전신성홍반성루푸스에 동반된 급성 비결석담낭염

김윤아 ( Yun A Kim ),이신석 ( Shin Seok Lee ),정해권 ( Ha Gwon Juhng ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.4

Several gastrointestinal complications have been reported in association with systemic lupus erythematosus (SLE), including mesenteric vasculitis, bowel perforation, gastric or duodenal ulcer, necrotizing enterocolitis, spontaneous peritonitis, pancreatitis, spontaneous rupture of the liver or spleen and hepatomegaly. Acute acalculous cholecystitis is a rare disease that occurs in only 5~10% of patients with acute cholecystitis, typically in seriously ill patients. Reports of gallbladder diseases in patients with SLE are very scarce. Only seven cases of SLE complicated with acalculous cholecystitis have been reported so far, but not in Korea. Six cases were treated surgically by cholecystectomy or cholecystostomy, and one case was treated without surgical intervention. We added a case in which acute acalculous cholecystitis accompanying SLE was treated successfully by corticosteroid without surgical intervention.

통풍과 동반된 칼슘피로인산 (Calcium Pyrophosphate Dihydrate) 결정침착 질환의

정해권 ( Ha Gwon Juhng ),이신석 ( Shin Seok Lee ),김윤아 ( Yun A Kim ) 대한류마티스학회 2002 대한류마티스학회지 Vol.9 No.2

Calcium pyrophosphate dihydrate (CPPD) crystal deposition disease has various patterns of clinical manifestation ranging from an absence of symptoms to a severely destructive arthropathy. A number of metabolic diseases and physiologic stresses, such as aging and trauma, have been associated with CPPD crystal deposition. The coexistence of CPPD crystal deposition disease and gout varies from 2 to 8%. The mechanism of this association has been postulated to be a predisposition to the local deposition of pyrophosphate due to the presence of urate crystal, perhaps acting as a nucleating agent or factor which predispose to the deposition of both kinds of crystals. In Korea, although it is considered that CPPD crystal deposition occasionally coincides monosodium urate crystal, it has not been reported so far. We report a case with CPPD crystal deposition disease combined with gout in a patient who has suffered from osteoarthritis, which is diagnosed by polarized microscopic examination of synovial fluid.

루푸스에서 유리형 Fas는 질병의 활성도를 반영하는가?

김윤아 ( Yun A Kim ),이신석 ( Shin Seok Lee ),박미정 ( Mi Jeong Park ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.1

Objective: To determine whether levels of soluble Fas (sFas) are elevated in patients with systemic lupus erythematosus (SLE) and correlated with clinical disease activity. Methods: Serum samples were obtained from 62 SLE patients and 39 normal controls. We measured sFas levels using an enzyme-linked immunosorbent assay. Disease activity variables including Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) were examined. Results: The mean age of the patients was 32.1 years (range 13∼60 years) and the mean disease duration was 3.0 years (range 0.2∼10.0 years); 1 patient was male (1.6%). The median serum sFas concentration was 610.0pg/ml for SLE patients and 292.9pg/ml in controls. The serum sFas concentration was significantly higher in SLE patients than in controls (p<0.001). The Receiver Operating Characteristics (ROC) curve showed that the area under the curve for sFas was 0.911. Using the cut-off value of 370.5pg/ml, the sensitivity was 0.871 and the specificity was 0.846. However, we could not find any correlation between sFas levels and disease activity variables such as SLEDAI, complement, and anti-dsDNA antibodies. There was no significant difference in sFas levels between active (SLEDAI≥8) and inactive (SLEDAI<8) SLE patients. Conclusion: These results suggest that sFas levels are elevated in SLE patients, but sFas does not reflect disease activity.

난소암에서 IGF-2 와 H19 gene 의 promoter 이용과 발현정도의 변화

김윤아 ( Kim Yun A ),이찬 ( Lee Chan ),김승조 ( Kim Seung Jo ),이선영 ( Lee Seon Yeong ),김인호 ( Kim In Ho ),나영정 ( Na Yeong Jeong ),정상근 ( Jeong Sang Geun ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.3

목적 : 난소암의 발암기전과 Insulin-like growth factor-2 (IGF-2), 그리고 H19 gene의 imprinting 상태, promoter 이용과 발현정도와의 연관성을 알아보기 위해, 본 연구는 8예의 정상난소조직, 11예의 양성난소종양, 9예의 경계성 종양과 20예의 악성종양 조직을 대상으로 실험하였다. 연구 방법 : 등록된 조직의 IGF-2와 H19을 PCR-RFLP 방법을 사용하여 이형접합성 분석을 하였으며, IGF-2의 promoter 특이발현을 살펴보고자 RT-PCR을 사용하였다. 결과 : 각 조직에서 IGF-2의 LOI (loss of imprinting)은 각각 정상 난소조직 (50%)> 양성종양 (60%) > 난소암조직 (71%) > 경계성 종양 (77%) 순으로 나타났다. H19 gene의 LOI는 정상과 양성종양의 난소조직에서는 관찰되지 않았고, 경계성 종양에서 100% (3/3), 악성종양에서 40% (2/5)로 나타났다. 각각의 난소조직에서 promoter P1, P2, P3와 P4의 이용도는 서로 다른 양상을 보였다. 암조직에서는 상대적으로 promoter P1, P2 이용이 높았으나 promoter P3 이용은 낮았다. 난소암조직에서 IGF-2의 발현은 높게 나타났으나 H19의 발현은 정상조직에 비해 낮음을 보였다. 결론 : 이상의 결과들을 보면 IGF-2의 LOI, promoter 사용의 deregulation IGF-2와 H19 발현정도의 변화가 난소암의 발생과 진행에 연관이 있으리라 생각된다. Objective : To establish the possible role of imprinting in ovarian cancer, we determined the imprinting status of both IGF-2 and H-19 genes in ovarian cancer, borderline tumors of ovary, benign ovarian tumor and normal ovarian tissues. Methods : An allelictyping assay was performed using a PCR-RFLP-based method for identification of heterozygous informative cases. The usage of Insulin-like growth factor-Ⅱ (IGF-2) promoters was examined by RT-PCR using promoter-specific primers. The mRNA expression of IGF-2 and H19 was quantified using a densitometer. Results : Loss of imprinting (LOI) of IGF-2 was observed in the order of borderline tumor (77%)>cancer (71%)>benign tumor (60%)>normal ovarian tissues (50%) respectively. And the LOI of H19 gene was not detected in the normal and benign tissues but observed in the borderline tumor and cancer tissues, respectively. The usage of promoter P1, P2, P3 and P4 were observed different pattern in normal, benign tumor, borderline tumor and cancer tissues. The activity of mRNA expression of promoter P4 was higher than other promoters. The cancer tissues predominantly used promoter P1, P2 with relative silencing of the promoter P3. The ovarian cancer tissues showed the higher expression levels of the IGF-2 but a down-regulation of the H19 relative to normal tissues. Conclusion : These results suggest that LOI, deregulation of the IGF-2 promoters, and the altered expression levels of the IGF-2 and h19 gene might be associated with progression of ovarian cancer.