사염화탄소 장기투여로 초래된 간세포의 소포체 변화에 관한 전자현미경적 연구

김유문,손태중,홍순덕 中央醫學社 1972 中央醫學 Vol.23 No.5

The author was carried out light and electron microscopic investigation to delineate the intriguing endoplasmic reticulum changes of hepatocyte after long term exposure to carbon tetrachloride. The female Sprague-Dawley albino rats were received subcutaneous injections-twice a week for thirteen weeks, of 0. 13 ml. 50 per cent solution of carbon tetrachloride in olive oil per 100 gm of body weight. In each instance, the rats were sacrificed three days after the last injection. The control rats were not received any treatment. The results obtained are as follow: 1) The author's findings on fine structure of endoplasmic reticulum in hepatocytes of normal rats are almost similar to that of other investigators. 2) The morphological effects of long-term administration of carbon tetrachloride upon the endoplasmic reticulum of hepatocyte are summarized as below. I) Two types of morphologically altered hepatocytes are encountered. One of these, corresponding to slightly basophilic cell in the H-E preparation, is characterized by proliferation of rough-surfaced endoplasmic reticulum but slightly reduced as compare with normal hepatocyte. And the other is characterized by irregular arranged, dilated often cystic ally and reduced in reticulum with disappearance of RNP particles from its membrane which is correspond to eosinophilic and lipid-containing cell in the H-E presparation. However various degree between these two extreme types are frequently-encountered. ii) The elongated profiles fregment into short tubles or flattened vesicles resembling honey-comb appearance frequently in lipid-laden cells. These cells sometimes have enlarged Golgi. 3) These altered endoplasmic reticulum are belived to be degenerative changes after prolong exposure to carbon tetrachloride.

2 종의 Hexachlorophene 유도체가 건흡충중의 간세포에 미치는 영향에 관한 전자현미경적 연구

김유문 慶北大學校 醫科大學 1973 慶北醫大誌 Vol.14 No.1

저자는 가토에 참붕어의 간흡충 피낭유충을 증감염시켜 이에 대한 형태학적 변화를 검색하고 아울러 간흡충의 구충제로 알려진 MTPA와 MTMA를 상기한 간흡충증의 가토에 투여하고 간세포에 일어난 변화를 형태적으로 관찰하여 얻은 성적을 요약하면 다음과 같다. 간흡충 감염 가토와 이에 MTPA 및 MTMA를 투여한 가토들의 육안 및 광학현미경 소견은 타 연구자들의 결과와 큰 차이가 없다. 즉 육안으로는 미세과립상을 나타내는 표면을, 광학현미경으로 간세포에는 공포성 변성, 지방변화, 재생세포의 출현 및 재생 결절을 그리고 담관에는 그 상피와 주위 결체조직의 증식등이었다. 전자현미경으로는 단백합성, 에너지 생산 및 당대사에 관여하는 세포내 소기관에 주적인 변화가 일어났는데 단백합성 소기관에는 rER의 단열, 확장 및 ribosome의 탈락을, 에너지 생산의 소기관에는 mitochondria의 변연의 불규칙과 cristae의 변형을 그리고 당대사에 관여하는 소기관에는 sERdml 증식과 glycogen과립의 감소등이 각각 그 특징이였다. 그밖에 핵 변연의 불규칙, lipid droplet 및 lysosome의 증가, Golgi complex의 위축 그리고 FCD의 출현등도 있었다. 상기 변화들은 MTPA나 MTMA를 투여한 실험동물이 그렇지 않는 실험동물보다 약간 경하게 또 투여후 시일이 길수록 경감되는 경향을 나타내었다. 이상의 연구결과로 보아 간흡충은 간세포내 소기관들중 단백질합성, 에너지 생산, 그리고 당대사에 관여하는 구조물에 주 변화를 야기하나, 이러한 간세포에 MTPA나 MTMA를 투여하면 이들 변화가 다소 경미하여지는 경향이었다. The author studied the effect of experimentally induced clonorchiasis on the fine structures of liver cells, and effects of anticlonorchial agents, 2-2'-methylene bis (3,4,6, trichlorophenoxy acetic acid) (MTPA) and 2-2'-methylene bis (3,4,6 trichlorophenozy methyl-propionic acid) (MTMA) on the fine structure of liver cells in rabbits experimentally infested with Clonorchis Sinensis. Gross and light microscopic changes of the liver were similar to the results of other investigators. Electron microscopically, the main changes due to the infection of Clonorchis sinensis were observed in the organells with related to protein synthesis, energy production and carbohydrate metabolism. They were consisted of fragmentation of rough ER and ribosomal shedding, irregular contour of mitochondria with loss of cristae, and proliferation of smooth ER and decrease of glycogen particles. The other findings were irregulatrity of nuclear margin, increase of lipid droplets and lysosomes, atrophy of Golgi complex and appearence of focal cytoplasmic degradation (FCD). It is likely that MTPA and MTMA treated rabbits showed milder morphological changes of liver than nontreated animals.

제27차 학술대회 : 78. 외상성 췌장손상

김유문,추연만,윤건호 대한외과학회 1975 대한외과학회 학술대회자료집 Vol.- No.-

외상성 췌장손상

김유문(Yu Moon Kim),장수일(Soo Il Chang),윤건호(Kun Ho Youn) 대한외과학회 1976 Annals of Surgical Treatment and Research Vol.18 No.2

外科的 甲狀腺疾患 296例에 對한 臨床的 考察

金裕文(Yu Moon Kim),黃一愚(Il Woo Whang) 대한외과학회 1975 Annals of Surgical Treatment and Research Vol.17 No.10

제26차 학술대회 : 28. 갑상선환자 756예의 임상적 고찰

이승기,김유문,황기우 대한외과학회 1974 대한외과학회 학술대회자료집 Vol.- No.-

Methylene bis (3,4,6-Trichlorophenoxy Acetic Acid) (MTPA)와 Deoxycholic Acid(Decholate)가 Biliverdin 排泄에 미치는 영향

朴震洪,金裕文,金鍾石 慶北大學校 醫科大學 1974 慶北醫大雜誌 Vol.15 No.1

Methylene bis(3.4.6-Trichlorophenoxy Acetic acid)(MTPA)와 Deoxycholic acid(Decholoate)가 Biliverdin排池에 미치는 영향

朴震洪,金裕文,金鍾石 慶北大學校 醫科大學 1974 慶北醫大誌 Vol.15 No.1

Methylene bis (3,4,6-trichlorophenoxy acetic acid) [MTPA] 및 deoxycholic acid가 膽汁內의 biliverdin의 排泄에 對한 作用을 보기 위하여 家兎膽管에 polyethylene管을 揷入하여 膽汁을 採取하면서 MTPA 및 deoxycholic acid를 耳靜脈으로 投與하고 採取한 膽汁에 含有된 biliverdin을 測定한바 아래와 같은 結果를 얻었다. 1. Deoxycholic acid는 膽汁의 biliverdin排泄에 對하여 顯著한 作用을 보이지 않았다. 2. MTPA는 膽汁의 biliverdin의 排泄을 增加시켰다. The effects of MTPA and decholate on the biliary excretion of biliverdine from common bile duct were observed in the rabbits. 1. Bile flow was reduced either with medication, MTPA or decholate, or without 2. Amount of biliverdin excreted from the bile duct were increased in the group of MTPA, i.v., treatment but not in other groups.

韓國成人의 無酸素역치

박희명,김유문,김종석,송호영,조용근,박재용,정태훈 대한스포츠의학회 1995 대한스포츠의학회지 Vol.13 No.1

한국인의 AT 및 AT/VO_2 max의 성별, 연령별 참고치를 마련하기 위하여 20대에서 50대 이상까지의 성인남자 471명과여자 335명, 합계 806명을 대상으로 Bruce 법으로, 증상제한성 최대운동부하검사를 실시해서 전사화된 V-slope 법으로 AT를 구하여 그 희귀방정식과 AT/VO_2 max를 산출하였다. 또한 답차운동과 EG 운동으로 측정한 AT의 희귀방정식을 비교해 보기 위하여 20대와 30대의 남녀 78명에서는 EG 두가지를 이용한 운동부하 검사를 실시하여 AT의 희귀방정식을 구하였다. 답차운동으로 구한 AT는 20대에서 가장 컸으나 연령의 증가에 따른 일정한 경향의 변화가 없었으며, AT/VO_2 max는 연령이 증가함에 따라 증가하는 경향이 있었는데 이는 연령에 따른 VO_2 max의 감소가 AT의 감소보다 더 컸기 때문이었으며, AT는 VO_2 max와 매우 높은 상관관계가 있었다. 남자는 여자에 비해 AT가 더 컸으나 AT/VO_2 max는 남녀간에 차이가 없었다. 그리고 성별과 신체적 조건을 변수로 한 AT의 희귀방정식은 AT (L/min)=1.0067 - 0.004×연령 + 0.0161×체중(㎏) - 0.3857×성별(남자=9, 여자=1) (r=0.67)이었고, 위의 변수에 VO_2 max를 포함시켜서 희귀방정식을 구하면 AT (L/min)= 0.4352+0.008×체중(㎏) + 0.2759×VO_2 max - 0.1792×성별(남자=0, 여자=1) (r=0.71)이었다. 한편 EG 운동으로 구한 AT의 희귀방정식은 성별과 신체적 조건을 변수로 하는 경우 AT(L/min)=1.4380 - 0.018×나이 + 0.0142×체중(㎏) - 0.7009×성별(남자=0, 여자=1) (r=0.67)이었으나, 이들 변수에 VO_2 max를 포함시킨 AT의 희귀방정식은 남녀를 합했을 경우 AT (L/min) = -0.0724 + 0.6499×VO_2 max (r=0.76)로서 답차운동으로 구한 그것과는 달리 VO_2 max만이 유일한 변수로 채택되었고 상관계수도 상당히 높았다. To establish the reference values of the anaerobic threshold (AT) by sex and age in healthy Korean adults, the symptom-limited maximal exercise test by Bruce protocol was carried out and the AT was determined by or with the aid of the computerized V-slope method, along with the VO_2 max, in 806 healthy Korean adults consisting of 471 men and 335 women aged from 20's to 60's. In addition, regression equations of the AT determined by the bicycle ergometer mode of exercise were computed in 78 men and women with their ages of 20's to 30's for comparison with those by Bruce protocol. The AT was the greatest in the age of 20's, the mean (±SD) being 2.034±0.4853 L/min and 1.349±0.2854 L/min in men and women, respectively. The AT/VO_2 max, however, was the least in the age of 20's, the mean (±SD) being 53.8±12.58% and 56.1±11.88% in men and women, respectively, and tended to increase with age, because of more marked decrease in the VO_2 max than in the AT with age. The AT in men was greater than that in women, but thee AT/VO_2 max was not significantly different between men and women. The regression equation for the AT using the anthropometric data and sex as variables was AT L/in)=1.0067 - 0.004 (age in year) + 0.0161 (weight in kg)-0.3857 (sex, coded 0 for males and 1 for females), (r=0.67), and that derived adding the VO_2 max to the above variables was AT (L/min)=0.4352+0.008 (weight in kg) + 0.2759 VO_2 max-0.1792(sex, coded 0 for males and 1 for females), (r=0.71). The regression equation of the AT determined by the bicycle ergometer mode of exercise was AT (L/min_= 1.4380 - 0.018 (age in year)+ 0.0142(weight in kg)-0.7009 (sex, coded 0 for males and 1 for females), (r=0.67), and that computed adding the VO_2 max to the variables, anthropometric data and, was AT (L/min)=-0.0724+0.6499 VO_2 max, (r=0.76), in which the VO_2 max was the only variable, in contrast to that by the treadmill mode of exercise.

2-2-Methylene bis (3, 4, 6-trichlorophenoxy acetic acid) (MTPA)의 체내흡수(體內吸收) 분포(分布) 및 배설(排泄)에 관(關)한 연구(硏究)

김종석,박준형,김유문,강영호,Kim, Chong-Suk,Pock, Joon-Hyoung,Kim, Yu-Moon,Kang, Yung-Ho 대한약리학회 1973 대한약리학잡지 Vol.9 No.1

It has been reported previously that 2,2-methylene bis(3,4,6-trichlorophenoxy acetic acid) (MTPA) is effective treating for clonorchiasis and less toxic to the hosts. In this studies the absorption, distribution and excretion of MTPA were observed. For this purpose $^{14}C-MTPA$ was synthesised from bis(2-hydroxy-3,5,6-trichlorophenoxyl) methan $^{14}C$ and administerd to the normal rabbit in a single dose of 10mg/kg IV or 20 mg/kg P.O. or to the Clonorchis infected rabbit in dose of 20 mg/kg/day for 6 days. Radioactivity in blood, tissue, bile, urine, feces and tissue of the fluke was measured after the drug was given. The concentration of MTPA in these samples were calcurated from the radioactivity. The result obtained as followes. 1. The increase in concentration of MTPA in blood and urine after oral administration of MTPA was so slow that the absorption of MTPA from the gastrointestinal tract appears very slow. 2. It is presumed that the excretion of MTPA also is slow because the reduction of MTPA concentration in blood after IV injection was very slow. 3. Large amount of MTPA was excreted from the bile. 4. During repeat dose of 20mg/kg/day for 6 days the concentration of MTPA in blood and tissue gradually increased. 5. The highest concentration of MTPA in the kidney and liver, heart, lung, spleen and muscle in decreasing order and the lowest concentration in the brain was noted. 6. During daily dose of 20 mg/kg of MTPA for 6 days of administration the concentration of MTPA gradually increased in urine and feces and the concentration of MTPA in feces was higher than of in urine. It appeares that MTPA take place enterophepatic circulation. 7. It is assumed that accumulation in large amount of MTPA in the liver and tissue of clonorchis, excretion of large amount from the bile is a favorable property of MTPA as a chemotherapeutic agent for clonorchiasis.