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식품 미생물 균총 연구를 위한 최신 마이크로바이옴 분석 기술
권준기(Joon-Gi Kwon),김선균(Seon-Kyun Kim),이주훈(Ju-Hoon Lee) 한국식품과학회 2019 식품과학과 산업 Vol.52 No.3
Rapid development of next-generation sequencing (NGS) technology is available to study microbes in genomic level. This NGS has been widely used in DNA/RNA sequencing for genome sequencing, metagenomics, and transcriptomics. The food microbiology area could be categorized into three groups. Food microbes including probiotics and food-borne pathogens are studied in genomic level using NGS for microbial genomics. While food fermentation or food spoilage are more complicated, their genomic study needs to be done with metagenomics using NGS for compositional analysis. Furthermore, because microbial response in food environments are also important to understand their roles in food fermentation or spoilage, pattern analysis of RNA expression in the specific food microbe is conducted using RNASeq. These microbial genomics, metagenomics, and transcriptomics for food fermentation and spoilage would extend our knowledge on effective utilization of fermenting bacteria for health promotion as well as efficient control of food-borne pathogens for food safety.
오덕철,김선균,주왕기,조동현,김우호,윤경민 江原大學校附設體力硏究所 1976 江原大學校附設體育科學硏究所論文集 Vol.- No.1
The water quality of the Hong-Cheon river was investigated during the winter season. The rate of contamination of the low tide was a little more than the high tide. But all values of the analyzed-items were similar to those in the clear water.
저강도 펄스형 초음파를 이용한 개의 슬개골 탈구 치료 효과
조성진,김남수,김화영,김선균,이성호,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
The effect of low-intensity ultrasonography on dislocation of the patella and fracture healing is controversial, and current management of that does not generally involve the use of ultrasound therapy. Nine patients with dislocation of the patella were treated with low-intensity ultrasonography(l5 mW/㎠, 1∼3.3 MHz) per 10-15 min/days, with an interval of 3 days during the last 3 weeks, at Family Animal Hospital(in Kunsan city). Ultrasound therapy may be beneficial to dislocation of the patella and healing. This finding is of considerable importance in that treatment with a low-intensity pulsed ultrasound signal may reduce healing time and could yield substantial cost savings and decreases in disability associated with delayed healing of dislocation of the patella.
급격한 온도 변화에 순응한 모래무지(Peudogobio esocinus) 젖산탈수소효소 활성과 동위효소의 변화
김재범,김선균,염정주 청주대학교 산업과학연구소 2003 産業科學硏究 Vol.21 No.1
Changes of activities and isozymes of lactate dehydrogenase(EC 1.1.1.27, LDH) in Pseudogobio esocinus acclimated to acute change of temperature were studied. The LDH activities of heart, liver, eye, and brain acclimated to 10℃ were detected higher level than those acclimated to 20℃. And the LDH activity of skeletal muscle showed higher level when acclimated to 20℃. And acclimation temperature had no significant effect on the LDH activity of the heart. When the temperature was acutely changed, the variation of the LDH activity had maximum state within 2 hours. The activity of subunit which has higher activity among subunit A and B was decreased and that of the subunit having lower activity was increased. The metabolism of liver tissue was regulated by the increase of C₄ isozyme activity and the decrease of A₄ isozyme. LDH was played role as lactate oxidase in skeletal muscle, heart, and liver, and as pyruvate reductase in eye and brain tissue. As a result, the change of LDH activity and isozyme to acute increase of temperature might be the acclimation process to the control of metabolism with surroundings.