Glucocorticoid Regulation of Gene Expression in Hippocampal CA3 and Dentate Gyrus

김동섭,안순철,김영진,박병권,안용태,김지연,허송욱,Kim, Dong-Sub,Ahn, Soon-Cheol,Kim, Young-Jin,Park, Byoung-Keun,Ahn, Yong-Tae,Kim, Ji-Youn,Kyoji, Morita,Her, Song Korean Society of Life Science 2007 생명과학회지 Vol.17 No.3

글루코코르티코이드는 해마 조직에서 대사, 스냅신 형성, apoptosis, 신경세포 생성과 세포에 있어서 수지상의 형태에 영향을 준다. 글루코코르티코이드 호르몬에 의한 해마조직의 생리학적 조절을 이해하기 위하여, CA3와 DG (dentate gyrus)에서 유전자 발현에 대하여 조사하였다. Lewis 쥐에 9.5mg의 코르티코스테론 알약 또는 플라시보 알약을 20일 동안 처리한 후에 올리고머 유전자 칩을 이용하여 유전자 발현을 조사 하였다 (Rat Neurobiology U34 Arrays, Affymetrix). 플라시보 알약을 처리한 쥐에서 32 유전자들이 DG보다 CA3에서 발현이 높았으며, 3개 유전자는 CA3보다 DG에서 높은 발현을 보였다. 코르티코스테론 호르몬 처리에 의한 해마조직의 유전자 발현 형태는 해부학적 구조의 차이를 보였다. 특히, CA3에서 6개의 유전자와 DG에서 41 개의 유전자가 호르몬에 의하여 조절 받았으며, 이중 43개의 유전자가 상승 발현하였으며, 4개의 유전자가 하강 발현 하였다. 이들 유전자를 기능에 의해 분류하면, 13개의 신경전달물질관련 유전자, 5개의 이온채널,4개의 전사인자, 3개의 neurotrophic인자, 1개의 각 사이토카인과 apoptosis관련 유전자, 그리고 5개의 스냅신형성관련 유전자가 해마조직에서 발현의 변화를 보였다. 특히, 스트레스 호르몬에 의하여 CA3에서 BDNF의 감소를 볼 수 있었다. 이러한 결과는 호르몬에 의하여 해마구조의 생리학적인 다양성을 내포 하고 있다. Glucocorticoids (GCs) alter metabolism, synaptogenesis, apoptosis, neurogenesis, and dendritic morphology in the hippocampus. To better understand how glucocorticoids regulate these aspects of hippocampal biology, we studied gene expression patterns in the CA3 (Hippocampal pyramidal cell field CA3) and dentate gyrus (DG). Litter-matched Lewis inbred rats treated for 20 days with either 9.5 mg per day sustained-release corticosterone or placebo pellets were compared with high-density oligonucleotide microarray analysis (Rat Neurobiology U34 Arrays, Affymetrix). In placebo-treated rats, 32 genes were expressed at greater levels in CA3 than DG, whereas 3 genes were expressed at great levels in DC than CA3. Regional differences were also apparent in corticosterone-induced changes in the hippocampal transcriptome. Six genes in CA3 and 41 genes in DC were differentially regulated by corticosterone. As per the glucocorticoid effects on gene transcription in the brain, forty three of these genes were upregulated, and 4 genes were downregulated. Genes differentially expressed in hippocampus included those for 13 neurotransmitter proteins, 5 ion channel related proteins, 4 transcription factors, 3 neurotrophic factors, 1 cytokine, 1 apoptosis related protein, and 5 genes involved in synaptogenesis. Interestingly, GCs can have suppressive effects on brain BDNF mRNA transcription, one of the neurotrophic factors. These results indicate the diversity of targets affected by chronic exposure to corticosterone and highlight important regional differences in hippocampal neurobiology.

삼중압 열회수 증기발생기와 중기터빈 시스템의 열설계 해석

김동섭,이봉렬,노승탁,신흥태,전용준,Kim, Dong-Seop,Lee, Bong-Ryeol,No, Seung-Tak,Sin, Heung-Tae,Jeon, Yong-Jun 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.3

A computation routine, capable of performing thermal design analysis of the triple-pressure bottoming system (heat recovery steam generator and steam turbine) of combined cycle power plants, is developed. It is based on thermal analysis of the heat recovery steam generator and estimation of its size and steam turbine power. It can be applied to various parametric analyses including optimized design calculation. This paper presents analysis results for the effects on the design performance of heat exchanger arrangements at intermediate and high temperature parts as well as steam pressures. Also examined is the effect of steam sources for deaeration on design performance.

단축가스터빈의 설계점 및 부분부하 성능해석 프로그램 개발

김동섭,노승탁,Kim, Dong-Seop,No, Seung-Tak 대한기계학회 1996 大韓機械學會論文集B Vol.20 No.7

This paper describes the development of a general program for the design and part load performance analysis of single-shaft-heavy-duty gas turbines. Efforts are made to fully represent the real component features by the characteristic models and special emphasis is put on the modeling of cooled turbine stages. The design analysis routine is applied to simulate the performance of current gas turbines and its appropriateness for system analysis is validated. Meanwhile, the component parameters of real engines which describe the technology level are obtained. The program is extended to predicting the part load operation of gas turbines with the aid of models for the off-design characteristics of compressor, turbine and other main components. Part load simulation can be carried out only with limited numbers of input data. It is demonstrated that the program accurately estimates the part load characteristics of real turbines.

정익 후연의 냉각유체분사를 포함한 축류터빈단의 성능해석

김동섭,김재환,노승탁,Kim, Tong Seop,Kim, Jae Hwan,Ro, Sung Tack 대한기계학회 1999 大韓機械學會論文集B Vol.23 No.7

In this work, an aerothermodynamic calculation model for cooled axial flow turbine blades with trailing edge ejection is suggested and a mean line performance analysis of a turbine stage with nozzle cooling is carried out. A unique model regarding the interaction between coolant and main gas is proposed, while existing correlations are adopted to predict viscous loss and blade outflow angle. The interactions considered are the heat transfer from main gas to coolant and the temperature and pressure losses by the mixing of two streams due to the trailing edge coolant ejection. For a stator blade without ejection, trailing edge loss calculated by the trailing edge analysis is compared with that calculated by loss correlation. The effect of heat transfer effectiveness of coolant passage on the mixing loss is analyzed. For a model turbine stage with nozzle cooling, parametric analyses are carried out to investigate the effect of main design variables(coolant mass flow ratio, temperature and ejection area) on the stage performance.

가스터빈의 설계 성능과 부분부하 성능 사이의 상관관계 분석

김동섭,Kim, Dong-Seop 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.8

This study analyzed the dependence of part load performance of simple cycle gas turbines on their design performance. Various parametric calculations were carried out to examine effects of design temperature ratio, pressure ratio and component efficiencies using a simplified analysis. In addition, a more practical analysis was done for realistic design conditions with the aid of a comprehensive performance analysis program. The results show that gas turbines with higher design performance exhibit less efficiency degradation during part load operation. The influence of power control method (fuel only centre) and air flow control) on part load performance was examined as well.

한전 해외 배전자동화사업 추진현황 및 향후전망

김동섭,Kim, Dong-Seop 대한전기협회 2009 전기저널 Vol.- No.385

고효율 단결정 실리콘 태양전지

김동섭,조은철,조영현,민요셉,이수홍,Kim, D.S.,Cho, E.C.,Cho, Y.H.,Ebong, A.U.,Min, Y.S.,Lee, S.H. 한국태양에너지학회 1997 한국태양에너지학회 논문집 Vol.17 No.2

단결정 실리콘 태양전지는 PESC(Passivated Emitter Solar Cell), PERC(Passivated Emitter and Rear Cell), Point Contact Cell, PERL(Passivated Emitter and Rear Locally-Diffused Cell) 형태로 기술적인 발전을 해왔다. BCSC(Buried Contact Solar Cell)는 낮은 제조 단가로 높은 효율을 얻을 목적으로 개발되었으며 개량된 형태인 DSBC(Double Sided Buried Contact Cell)는 양면으로 빛을 흡수할 수 있는 장점이 있다. Since PESC(passivated emitter solar cell) was developed in 1985, high efficiency silicon solar cell technology based on planar technology has been improved in the order of PERC, Point Contact Solar Cell, PERL. BCSC and DSBC, which do not require photolithography, are expected to replace commercial screen printed cells because of its potential for low cost and high efficiency. In this paper, history and characteristics of each type of cells are reviewed.

축류터빈의 관통유동해석 - 다유선해석과 평균반경해석의 비교분석 -

김동섭,Kim, Tong Seop 대한기계학회 1998 大韓機械學會論文集B Vol.22 No.8

A throughflow analysis program for axial flow turbines is constructed, which can handle not only the two-dimensional multi-streamline (streamline curvature) method but also the one-dimensional mean line method. Calculations are performed for single stage and multi-stage axial flowturbines. For a wide operating range, the performance and flow field calculated by the present streamline curvature method are close enough to the test data. It is also revealed for the single stage turbine that the present analysis leads to far better correspondence with the experiment than other researchers" throughflow analyses. A special focus is put on the comparison of the results between the streamline curvature analysis and the mean line analysis. It is found that the mean line analysis can not predict the performance for highly off-designed conditions as accurately as the streamline curvature method, which shows the importance of considering the spanwise variation of loss and flow.

컬러화보-공룡

김동섭,Kim, Dong-Seop 한국과학기술단체총연합회 1994 과학과 기술 Vol.27 No.4

액화천연가스의 냉열을 이용한 가스터빈의 성능향상

김동섭,노승탁,이우일,최만수,고상근,Kim, Tong Seop,Ro, Sung Tack,Lee, Woo Il,Choi, Mansoo,Kauh, Sang Ken 대한기계학회 1999 大韓機械學會論文集B Vol.23 No.5

This work describes analysis on the effect of inlet air cooling by the cold energy of liquefied natural gas(LNG) on the performance of gas turbines. Gas turbine off-design analysis program to simulate the influence of compressor inlet temperature variation is prepared and an inlet air cooler is modeled. It is shown that the degree of power augmentation is much affected by the humidity of inlet air. If the humidity is low enough, that is the water content of the air does not condense, the temperature drop amounts to $18^{\circ}C$, which corresponds to more than 12% power increase, in case of a $1350^{\circ}C$ class gas turbine with methane as the fuel. Even with 60% humidity, about 8% power increase is possible. It is found that even though the fuel contains as much as 20% ethane in addition to methane, the power improvement does not change considerably. It is observed that if the humidity is not too high, the current system is feasible oven with conceivable air pressure loss at the inlet air cooler.