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김대혁,박승문,김범태,양문식,황기준,Joong-Keun Lee,Jung-Ae Kim,Hea-Jong Chung 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5
The cultural characteristics of the fungus Cladosporium phlei were assessed in order to develop an improved method for the production of the fungal pigment, phleichrome, which is an intermediate in the production of a photodynamic therapeutic agent. The growth of C. phlei, as measured by the hyphal growth rate and increase in biomass, varies significantly depending on the culture media utilized (V8 juice-based medium proved optimal for both growth rate and biomass increase). However, even on a V8 juice plate, the growth of C. phlei occurred slowly and in a limited fashion, in that the colony covered only 75% of the agar surface after more than 4 weeks of cultivation at 20℃. Supplementations of glucose, fructose, galactose, and sucrose increased both hyphal expansion and mass production, whereas supplementations of other carbon sources, including glycerol and sorbitol, exerted no detectable effects. The effect of inorganic nitrogen supplementation was negligible, whereas organic nitrogen evidenced significant effects, with enhanced growth with malt extract and growth inhibition with yeast extract and tryptone. Sporulation was enhanced under conditions of continuous light, and a minimum of 103 spores per mL of liquid media was found to be necessary for the optimal mass increase. A simple extraction procedure was established in order to isolate the deep red pigment which was subsequently identified as phleichrome via NMR analysis. When C. phlei was cultured on V8 medium containing 5% glucose and 2% malt extract, the quantity of mycelial mass was estimated as 20.6 g (dry weight) per liter of culture. The expected phleichrome yields from the mycelia and culture filtrates were estimated to be 43 and 2 mg/L, respectively.
Expression of Fungal Phytase on the Cell Surface of Saccharomyces cerevisiae
김대혁,박승문,양문식,Ae-Young Mo,Yun-Sik Kim 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6
Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals, and reduces the phosphorus pollution of animal waste. We have engineered the cell surface of the yeast, Saccharomyces cerevisiae, by anchoring active fungal phytase on its cell wall, in order to apply it as a dietary supplement containing bioconversional functions in animal foods and a whole cell bio-catalyst for the treatment of waste. The phytase gene (phyA) of Aspergillus niger with a signal peptide of rice amylase 1A (Ramy1A) was fused with the gene encoding the C-terminal half (320 amino acid residues from the C-terminus) of yeast a-agglutinin, a protein which is involved in mating and is covalently anchored to the cell wall. The resulting fusion construct was introduced into S. cerevisiae and expressed under the control of the constitutive glyceraldehydes-3-phosphate dehydrogenase (GPD) promoter. Phytase plate assay revealed that the surface-engineered cell exhibited a catalytically active opaque zone which was restricted to the margin of the colony. Additionally, the phytase activity was detected in the cell fraction, but was not detected in the culture medium when it was grown in liquid. These results indicate that the phytase was successfully anchored to the cell surface of yeast and was displayed as its active form. The amount of recombinant phytase on the surface of yeast cells was estimated to be 16,000 molecules per cell.
주익이 손상된 전익형 무인기를 위한 신경회로망 적응제어기법에 관한 연구
김대혁(DaeHyuk Kim),김낙완(Nakwan Kim),석진영(Jinyoung Suk),김병수(Byungsoo Kim) 한국항공우주학회 2013 韓國航空宇宙學會誌 Vol.41 No.5
무인항공기가 외형손상을 입는 경우, 비행역학 특성이 변하기 때문에 손상 이전 설계된 제어기는 더 이상 안정적인 제어성능을 보장하지 않는다. 본 논문에서는 주익의 손상이 일어난 무인항공기에 대해서도 강건한 제어성능을 보장하는 신경회로망 적응제어기법을 소개한다. 구동기의 특성에 의한 제어기의 성능저하를 방지하기 위해 Pseudo Control Hedging(PCH)를 추가적으로 사용하였다. 기체고정좌표계의 중심이 항공기의 무게중심에 위치하지 않는 비대칭 동역학을 사용하였으며, 전익형 무인기를 대상 비행체로 하였다. 날개가 손상되지 않은 모델과 손상된 모델의 풍동시험을 통해 얻은 공력데이터를 이용하여 시뮬레이션을 수행하였다. 시뮬레이션의 결과를 통해 제안된 제어기법이 주익의 손상이 발생한 항공기에 대해서도 여전히 안정적인 조종성능을 보장하는 제어기법임을 검증하였다. A damage imposed on an unmanned aerial vehicle changes the flight dynamic characteristics, and makes difficult for a conventional controller based on undamaged dynamics to stabilize the vehicle with damage. This paper presents a neural network based adaptive control method that guarantees stable control performance for an unmanned aerial vehicle even with damage on the main wing. Additionally, Pseudo Control Hedging (PCH) is combined to prevent control performance degradation by actuator characteristics. Asymmetric dynamic equations for an aircraft are chosen to describe motions of a vehicle with damage. Aerodynamic data from wind tunnel test for an undamaged model and a damaged model are used for numerical validation of the proposed control method. The numerical simulation has shown that the proposed control method has robust control performance in the presence of wing damage.
김진배 ( Jin Bae Kim ),정보영 ( Bo Young Joung ),김종윤 ( Jong Youn Kim ),김대혁 ( Dae Huyk Kim ),안신기 ( Shin Ki Ahn ),이문형 ( Moon Hyoung Lee ),김성순 ( Sung Soon Kim ) 대한내과학회 2006 대한내과학회지 Vol.71 No.3
Background: Although the determination of etiology is crucial for the management of ventricular tachycardia (VT), there have been few studies on the epidemiology and clinical characteristics of patients with ventricular tachycardia in Korea. Previous studies on Western populations have shown that ischemic VT is the most common type arrhythmia; however, the incidence of ischemic heart disease in Korea is lower than in Western culture. The purpose of this study was to determine the clinical characteristics and prognosis of Korean patients with VT. Methods: From January 1999 to December 2002 patients who were diagnosed with VT by ECG or confirmed by electrophysiological studies were enrolled. Hospital records were reviewed for the clinical characteristics which included: demographic features, underlying disease, clinical presentation, ECG features and prognosis in 136 patients who visited our emergency room or out patient clinic. Results: There were 100 men and 36 women (mean age 51.517.6). The most common presenting symptom was palpitation (56.6%), which was followed by chest pain and syncope. The most common type of VT was idiopathic VT (54.4%); ischemic VT was found in 29 cases (21.3%) and dilated or hypertrophic cardiomyopathy in 16 cases (11.8%). For all types of VT, the idiopathic VT showed the best prognosis and cardiac function (p<0.05). RFCA (Radiofrequency catheter ablation) was the best treatment option for treatment of idiopathic VT (p<0.05). Conclusions: These findings suggest that idiopathic VT may be the most common type of VT in Korean patients; it usually originates form either the right ventricular outflow tract (RVOT) or the LV. The response to treatment and prognosis was poor in VT patients who had underlying heart disease; however, the prognosis was excellent for those with idiopathic VT.(Korean J Med 71:276-284, 2006)