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식용식물자원으로부터 활성물질의 탐색-VI - 쑥갓(Chrysanthemum coronarium L.)으로부터 sterol의 분리 -
송명종,홍윤희,김동현,김대근,정인식,이윤형,김성훈,박미현,김인호,권병옥,백남인,Song, Myoung-Chong,Hong, Yoon-Hee,Kim, Dong-Hyun,Kim, Dae-Keun,Chung, In-Sik,Lee, Youn-Hyung,Kim, Sung-Hoon,Park, Mi-Hyun,Kim, In-Ho,Kwon, Byoung-Mog,Baek, 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.4
쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. EtOAc 분획으로부터 silica gel column chromatography를 반복하여 3종의 sterol을 분리하였다. 각각의 화학구조는, NMR및 MS등의 스펙트럼을 해석하여, $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-3-one$ (3)으로 동정하였다. 화합물 2와 3은 육상식물에서는 처음으로 분리되었다. The aerial parts of tile Garland (Chrysanthemum comnarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-ol-3-one$ (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.
식용식물자원으로부터 활성물질의 탐색-6, 쑥갓(Chrysanthemum coronarium L.)으로부터 sterol의 분리
송명종 ( Song Myeong Jong ),홍윤희 ( Hong Yun Hui ),김동현 ( Kim Dong Hyeon ),김대근 ( Kim Dae Geun ),정인식 ( Jeong In Sig ),이윤형 ( Lee Yun Hyeong ),김성훈 ( Kim Seong Hun ),박미현 ( Park Mi Hyeon ),김인호 ( Kim In Ho ),권병옥 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4
The aerial parts of the Garland (Chrysanthemum coronarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as stigmast-5-en-3β-ol (1, β-sitosterol), stigmast-4-en-6β-ol-3-one (2), stigmast-4-en-6α-ol-3-one (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.