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소의 정원세포에서 Matrix Metalloproteinase-9(MMP-9) 발현 양상 구명
곽태일,이원영,정하연,이승윤,송혁 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2016 동물자원연구 Vol.27 No.2
Identification of specific marker proteins in cells is useful for isolating cells and determining their cellular characteristics and functions. Based on our previous study showing that matrix metalloproteinase 9 (MMP-9) can be used as a marker for porcine spermatogonia, the expression pattern of MMP-9 was determined in both pre- (5-month old) and post-pubertal (11–month old) bovine testes. Histological analysis revealed that spermatogonia were located near the basement membrane in both testes, while spermatozoa were not detected in the 5-month old pre-pubertal bovine testes and epididymides. Mature spermatozoa were observed in the 11-month bovine testes and epididymides, and MMP-9 expression in 11-month old bovine testes was lower than 5-month old testes, according to reverse transcription-PCR and real-time-PCR data. To determine the specific expression sites of MMP-9 in the bovine testes, immunohistochemistry was performed. Expression of MMP-9 was observed in cells near the basement membrane of seminiferous tubules in both 5- and 11-month old testes. Furthermore, MMP-9 positive cells expressed protein gene product 9.5 (PGP9.5) and deleted in azoospermia (DAZL) that are already known as bovine spermatogonial stem cells markers. In the present study, MMP-9 expression was identified in both pre- and post-pubertal bovine spermatogonia expressing PGP9.5 and DAZL, and located near the basement membrane of seminiferous tubules. Thus, MMP-9 can be used as a marker for bovine spermatogonia, and may provide useful platforms for understanding the interaction between germ cells and extracellular matrix during spermatogenesis in the seminiferous tubules.
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지능형 홈서비스를 위한r-Sensor프로토콜설계 및 하드웨어 구현
곽태길,이범성,정진욱,진교홍,Kwak, Tae-Kil,Lee, Bum-Sung,Jung, Jin-Wook,Jin, Kyo-Hong 한국정보통신학회 2006 한국정보통신학회논문지 Vol.10 No.11
본 논문에서는 지능형 홈서비스를 위한 무선 센서 네트워크의 신뢰성 있는 데이터 전송을 위해 r-Sensor프로토콜을 설계하였다. r-Sensor 프로토콜은 간단한 경로 설정 방법과 중계 노드의 부담을 최소화시키는 알고리즘을 이용하여 Congestion이나 패킷 손실이 발생할 확률을 최소화시켜서 데이터 전달의 신뢰성과 노드의 공평성을 향상시키는 프로토콜이다. 제안된 경로설정 알고리즘은 망 관리 패킷(NM 패킷)을 사용하여 각 노드의 Upstream, Downstream 노드를 파악한다. 한편 패킷 손실 복원 알고리즘은 Aggregated-Nack를 사용한다. 제안된 알고리즘을 적용하기 위하여 본 논문에서는 댁내의 홈 게이트웨이 및 싱크노드 역할을 하는 지능형 홈 원격검침기(IHS-AMR)와 센서 노드의 하드웨어를 설계 및 구현하였다. IHS-AMR은 원격검침기의 고유 기능을 제공하며, 그 외에 센서 네트워크, 휴대 전화망, 인터넷 등 다양한 네트워크와의 연동을 통해 댁내의 안전 서비스를 제공한다. In this paper, we design the r-Sensor protocol for reliable data transmission in the Intelligent Home Service based on the wireless sensor network environment. The r-Sensor protocol improve the reliability of data transmission and node fairness using simple routing algorithm, congestion control, and loss recovery method that minimize the load of relay node. Reposed routing algorithm find out upstream and downstream nodes using the Network Management packet. Meanwhile, loss recovery algorithm uses the Aggregated-Nack. To apply supposed algorithm, the IHS-AMR(Intelligent Home Service - Automatic Meter Reader) and sensor node are designed and implemented in hardware. The IHS-AMR provides remote metering service and also offers home safety service by internetworking with sensor network, mobile phone network and internet.
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무선 센서네트워크에서 네트워크 수명 연장을 위한 클러스터 크기 조정 알고리즘
곽태길,진교홍,Kwak, Tae-Kil,Jin, Kyo-Hong 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.6
본 논문에서는 클러스터링 방식을 사용하는 무선 센서네트워크(WSN)에서 센서노드의 위치정보에 따라 클러스터의 크기를 다르게 설정하여, 네트워크의 수명을 향상시키는 알고리즘을 제안한다. 각 클러스터에서 수집된 정보는 클러스터 간(Inter-cluster) 통신으로 멀티 홉을 통하여 싱크노드에 전달된다. 싱크노드에 가까운 곳에 위치한 클러스터 헤더(CH)일수록 멀리 있는 클러스터 헤더보다 데이터 전달에 많이 관여하게 되어 에너지를 더욱 많이 소모하며 이로 인해서 네트워크의 수명이 줄어든다. 제안된 알고리즘은 싱크노드에 가까이 위치한 클러스터의 크기를 줄여서, 해당 클러스터 헤더에서의 에너지 소모를 최소화하고, 또한 싱크로 향하는 데이터들의 전송 경로를 다양화하여 클러스터 헤더의 통신 부담을 경감시킴으로써 네트워크의 수명을 연장시킨다. 제안된 알고리즘은 수학적인 분석 과정을 통해 성능을 검증하였다. In this paper, we propose an algorithm that improve network lifetime by adjusting cluster size according to location information of sensor node in wireless sensor network (WSN) using clustering algorithm. The collected sensing information by sensor nodes in each cluster are transferred to sink node using inter-cluster communications method. Cluster head (CH) that located nearby sink node spend much more energy than those of far from sink node, because nearer CH forwards more data, so network lifetime has a tendency to decrease. Proposed algorithm minimizes energy consumption in adjacent cluster to sink node by decreasing cluster size, and improve CH lifetime by distributing transmission paths. As a result of mathematical analysis, the proposed algorithm shows longer network lifetime in WSN.
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Adobe Illustrator 9.0을 이용한 의류용 텍스타일 디자인 개발 : 전통문양의 캐릭터화 사례를 응용하여
곽태기,양수영 복식문화학회 2001 服飾文化硏究 Vol.9 No.3
This study is focused on apparel textile design process applying pre-developed Korean traditional motifs. Since the fusion culture is in trend, Orientalism or Oriental look is the center of attraction in fashion. Under the circumstances, developing the traditional resources as sophisticated Korean-style is the goal of the BK21 team. This study makes a move to achieve the goal. As n method of accomplishing this study, 4 trendy motif samples such as stylized letter, butterfly, peony, and female face are selected after investigating fashion magazines and fashion related websites. As a result pre-developed Korean traditional motifs are elected and revised using Adobe Illustrator 9.0. The newly revised motifs are applied to the textile pattern repeat farm. The completed textile patterns are simulated on the shawls to show their usages as one of souvenir items since the year of 2001 is the year of "Visit Korea" Key worlds: Korean traditional motifs(한국전통문양), fashion culture(퓨전문화), traditional resources(문화유산), textile pattern repeat form(텍스타일 패턴의 반복 배열 방법), souvenir items(관광상품).
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곽태로 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.1
Korean hemorrhagic fever(KHF)was recognized for the first time in Korea in 1951 during the Korean War among United Nations troops but similar diseases to KHF had been reported in Russia, China and elsewhere. In 1976, Lee and Lee discovered a specific antigen of KHF and demonstrated that this antigen is the etiologic agent of KHF and perfected serologic test for diagnosis of the disease in 1978. The etiologic agent of KHF was named Hantaan virus after Hantaan river in 1980. Very recently, close etiological relations have been proved by serologic means between KHF in Korea and hemorrhagic nephroso-nephritis in the USSR, between KHF and nephropathia epidemica in Scandinavia, between KHF and epidemic hemorrhagic fever(EHF) in Japan and between KHF and EHF in China. The working group on HFRS at a WHO meeting in Tokyo February 1982 recommended that the above mentioned disesses with different names should be referred as "Hemorrhagic Fever with Renal Syndrome (HFRS)". Although there are several reports on transmission, sensitivities of various animals and electronmicroscopic findigns of Hantaan virus but no report was described on physiochemical properties of the virus. This is the first reports on the effect of temperature and pH on the infectivity of Hantaan virus in Apodemus mice. 1. The diameter of Hantaan virus was approximately 90nm. 2. Hantaan virus was inactivated rapidly at above 37℃. The infectivity of virus, however, was slowly decreased but preserved for about one month at 4℃. 3. The optimal pH for infectivity of Hantaan virus in Apodemus mice was 7.6 but it was inactivated completely a below pH 5.0. 4. It was demonstrated that BSS with 0.2% bovine albumin, pH 7.2∼7.6 was the most stable diluent for study of the infectivity of Hantaan virus.
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