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해조류 메탄올 추출물의 Prolyl Endopeptidase, Tyrosinase 저해 및 항응고 활성 스크리닝
이현진,김진희,이주현,김종식,곽상태,이경복,송경식,최병욱,이봉호,Lee, Hyun-Jin,Kim, Jin-Hui,Lee, Chu-Hyun,Kim, Jong-Sik,Kwak, Sang-Tae,Lee, Kyung-Bok,Song, Kyung-Sik,Choi, Byung-Wook,Lee, Bong-Ho 한국생약학회 1999 생약학회지 Vol.30 No.3
About forty sea weeds were screened for their inhibitory effects against prolyl endopeptidase, tyrosinase and thrombus coagulation. Out of them, methanolic extract of Ecklonia cava, Sargassum patens, Sargassum hemiphyllum, Sargassum thunbergii, Sargassum singgildianum, Hizikia fusiformis, and Ishige okamurae inhibited more than 90% of prolyl endopeptidase activity at 40 ppm. Sargassum siliquastrum and Ecklonia cava exhibited 51% and 76% of inhibitory activity against tyrosinase at 40 ppm, respectively. In APTT assay system, Sargassum singgildianum, Pterocladia capilacea and Hizikia fusiformis delayed coagulation of thrombus about two times (210, 211, and 198% over control at ca 367 ppm, respectively) and in TT assay, Lomentaria catenata, Laurencia okamurae, and Hizikia fusiformis did most effectively (216,197, and 251% at ca 367 ppm, respectively).
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노하석(Ha Seok Rho),서기범(Ki Beom Shur),이증훈(Jeung Hoon Lee),박장규(Jang Kyu Park),곽상태(Sang Tae Kwak),정희경(Hoe Kyung Jung) 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.11
Background:Telemedicine can be useful in dermatology in which there is a physical barrier between the dermatologist and patient or primary care giver. Objective:Our purpose of this study is to examine a usefulness of teledermatology system in Korean situation. Methods:We made store-and forward teledermatology system on world wide web for it's usefulness. We examined the difference of diagnostic accuracy, level of certainty, and quality of images of photographs and still digital images. Results:The merits of our teledermatology system are summarized as follows 1. It has more diagnostic accuracy than classic store-and forward system. 2. The real-time additional history taking and request of laboratory examinations are possible by chatting system between dermatologist and primary care giver. 3. Patient follow-up is easier than store-and forward system. The difference of diagnostic accuracy, level of certainty, and quality of images of photographs and still digital images are summarized as follows. 1. There is no difference of diagnostic accuracy between photographic readers and digital image readers. 2. There is no difference of level of certainty between photographic readers and digital image readers. 3. In assesment of quality of photographs, no difference was founded between photographic readers and digital image readers 4. The satisfaction of quality of photographs is very high in all groups. The average is 97.5%. Conclusion:This study provides evidence that our teledermatology system is useful in korean situation and still digital image can substitute historic photographs in teledermatology. (Korean J Dermatol 2000;38(11):1468~1474)
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水晶體 α-Crystallin의 Aggregation에 對한 Calcium의 影響에 關한 硏究
郭明憲,林圭,郭相太,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2
The elution profile of CaCI_2 treated and EGTA plus CaC1_2-treated soluble fraction protein of bovine lens are compared by Sephacryl S-300 chromatography, and then changes of protein patterns of CaCI_2-treated crystallin and EGTA plus CaCI_2-treated α-crvstallin fractions have been investigated by urea/polyacrylamide gel electrophoresis. 1. In the presence of 0.8 mM CaCI_2, 10 mM CaCI_2, or 10 mM CaCI_2 plus 20 MM EGTA, absorbance at 500 nm exhibited 0.039, 0.087, 0.040 respectively. 2. Physiologic concentration (0.8 mM) CaCI_2-treated, 10 mM CaCI_2-treated and 10 mm CaCI_2 plus 20 mM EGTA-treated soluble fractions were analyzed by Sephacryl S-300 chromatography. In the 0. 8 mM CaCI_2-treated proteins four peaks were eluted in the tube No. 17, 27, 31, 35 and identified as α, β_H, β_L, γ-crystallin, but eluted in the tube No. 16, 26, 30, 33 in 10 mM CaCI_2-treated protein, respectively. In presence of 10 mM CaCI_2 plus 10 mM EGTA, protein patterns exhibited the same. 3. Physiological concentration (0. 8 mM) CaC_2-treated α-crystallin was seperated ten subtractions by urea/polyacrylamide gel electrophoresis. In the 10 mM CaCI_2-treated α-crystallin, No. 9 subfraction was disappeared and No. 3B was newly appeared when compared with that of 0.8 mM CaCI_2 treated and 10 mM CaCI_2 plus 20 mM EGTA. 4. Absorbance and electrophoretic pattern of 10mM CaCI_2-treated native a-crystallin exhibited the same result of 0.8 mM CaCI_2-treated. 5. Ten inilimoles mercaptoethanol treated soluble fraction protein exhibited the same elution profile when compared with nontreated soluble fraction proteins. From the above results, it is suggested that the aggregation of a-crystallin of lens by calcium may be resulted to the interaction of (β,γ-crystallin, and not related the formation of disulfide bond.
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곽상태,이웅재,정회경,조인준,이증훈,박장규,서기범 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.12
Background:Most dermatologic diseases are diagnosed based on typical clinical and histopathologic features. Consequently, image databases for these diseases are necessary for educational and diagnostic purposes. Objective:To establish a general visual reference for dermatologic diseases and supporting materials for basic medical education. Methods:Approximately 2000 clinical images were selected from our archive of 50,000 slides. These images were digitized and catagorized according to the key system developed by the Department of Dermatology, Erlangen University with minor modification. The architecture and components of this image database are presented in this paper. Results:The database allows a search for appropriate dermatologic images according to diagnosis, localisation, and appearance of skin lesions. Conclusion:We have established a WWW (world-wide-web) based online dermatologic image database which can be used as a useful tool for general reference and for educational material.
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사람 태반조직 DNA Topoisomerase Ⅰ에 대한 Phosphorylation의 영향
곽상태,김승민,박종일,손미영,임규,황병두 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2
DNA topoisomerase I was prepared from human term placenta and the effects of phosphorylation/dephosphorylation to the enzyme activity have been investigated. hosphorylation of the enzyme by protein kinase'A or casein kinase II did not affect the activity of DNA topoisomerase I. But dephosphorylation of the enzyme by calf intestinal alkaline phosphatase decreased the DNA topoisomerase I activity. The inhibitory effects of camptothecin or 10-OH-camptothecin on the enzyme were not related to dephosphorylation of the enzyme. These results suggest that DNA topoisomerase I from human term placenta present in forms of phosphorylated, and regulation of the enzyme activity is related with phosphorylation/dephosphorylation of the enzyme.
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胎盤組織 Cytidine Deaminase에 關한 硏究
郭相太,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1
Cytidine deaminase(EC 3.5.4.5) was isolated two isoenzymes having molecular weight 1,000,000(CDase Ⅰ) & 90,000 (CDase Ⅱ), and patially purified 28 & 11 folds respectively by combination of amonium sulfate precipitation, sephacryl S-300 chromatography and adsorption to calcium phosphate gel, and then their properties are investigated. 1. pH optimum of CDase Ⅰ is around 7.2, but CDase Ⅱ exhibit broad from 5 to 9. 2. CDase Ⅱ, heat stable, remains 80% of activity against heat treatment at 80℃ for 5 minutes, but CDase Ⅰ is relatively heat labile. 3. The activity of CDase Ⅱ is decreased to 27% by addition of 3 units of proteinase in the reaction mixture, whereas CDase Ⅰ remains 69% of its activity. 4. The activity of both enzymes are inhibited by Cu^2+, increased by Ca^2+ and little affected by other cations. 5. Both enzymes are inactivated in prerence of 2mM p-hydoxymercuribenzoic acid, but previously 4mM mercaptoethanol added enzyme solution is not inactivated. 6. Polyamines markedly promote the activity of CDase Ⅰ, but, except spermidine, inhibit the activity of CDase Ⅱ. 7. The apparent Michaelis constant of both enzymes is identical for cytidine 5.5×10^(-5) M and for deoxycytidine 3.5×10^(-5) M. From the above results, CDase from human placenta is identified two isoenzymes and its properties are compared with those of the other tissue CDase.
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胎盤組織 Cytidine Deaminase에 關한 硏究 (Ⅰ) : Purification & Characterization
황병두,곽상태,임규,이재흔,백문기 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2
Cytidine deaminase has been partially purified from human term placenta approximately 26-fold by combination of ammoninm sulfate saturation, sephacryl S-300, DEAE-cellulose and hydroxyapatite chromatography, and then its properties have been investigated. The enzyme shows a broad optimum pH from 5 to 9 and is heat-stable being, almost remained its activity by heat treatment at 70℃ for 8 minutes. The enzyme activity is inhibited to 23% and 77% by 2 mM of Cu^2+ and Co^2+, respectively, but, other cations do not affect the enzyme activity. The apparant Michaelis constant for cytidine and deoxycytidine are 74 uM and 69 uM, respectively. Kinetic analysis of cytidine deminase in presence of 0.25 mM Cu^2+ shows that the nature of the inhibition to cytidine deaminase is competitive, considering that Vmax is independent while km value is increased. The enzyme activity is completely inhibited by 0. 5 mM of p-hydroxymecuribenzoicacid(PHMB)but all of the activity is recovered in adding mercaptoethanol. The enzyme activity is decreased about 50% in prescence of 5 mM of polyamines. The enzyme activity is mainly located in the nuclei (80%) and cytosol(16%) fractions. he molecular weight of the enzyme is about 770, 000.
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