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배열된 PCL 섬유 지지체의 근아세포 분화 촉진에 관한 연구
고웅현(Unghyun Ko),방현승(Hyunseung Bang),김미나(Mina Kim),박석희(Sukhee Park),양동열(Dongyol Yang),신현정(Jennifer H.) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
Fibrous scaffold and physical stimuli are known to enhance muscle development. In this study, we fabricated polycaprolactone (PCL) fibers in aligned or random arrangements by electrospinning. PCL fibers were spun towards either spaced collector or flat plate collector. C2C12 muscle cells (mouse myoblasts) were seeded on scaffolds coated with fibronectin (10μg/ml) and let grow until cells became confluent. Cells were then cultured for a week under horse serum condition. Immunofluorescence for skeletal myosin heavy chain, an indicator for muscle differentiation, showed enhanced differentiation of muscle cells on aligned fibers when compared to ones on random condition.. Synergetic effects of fiber alignments and DC electric field were further investigated to propose an appropriate condition for muscle differentiation for tissue engineering.
고웅현(Ung Hyun Ko),홍정우(Jungwoo Hong),신현준(Hyunjun Shin),김철웅(Cheol Woong Kim),신현정(Jennifer H. Shin) Korean Society for Precision Engineering 2015 한국정밀공학회지 Vol.32 No.8
For proper wound healing, dermal contraction and remodeling are critical; during the natural healing process, differentiated fibroblasts called “myofibroblasts” typically undertake these functions. For severe wounds, however, a critical mass of dermal matrix and fibroblasts are lost, making self-regeneration impossible. To overcome this impairment, synthetic wound patches with embedded functional cells can be used to promote healing. In this study, we developed a polydioxanone (PDO)-based cell-embedded sheet on which dermal fibroblasts were cultured and induced for differentiation into myofibroblasts, whereby the following combinatorial physicochemical stimuli were also applied: aligned topology, electric field (EF), and growth factor. The results show that both the aligned topology and EF synergistically enhanced the expression of alpha smoothmuscle actin (α-SMA), a key myofibroblast marker. Our proof-of-concept (POC) experiments demonstrated the potential applicability of a myofibroblast-embedded PDO sheet as a wound patch.