Cloning and protein expression of Actinobacillus actinomycetemcomitans cytolethal distending toxin subunit CdtA

고선영,정동근,유소현,김형섭,Ko, Sun-Young,Jeong, Dong-Keun,Ryu, So-Hyun,Kim, Hyung-Seop The Korean Academy of Periodontoloy 2007 Journal of Periodontal & Implant Science Vol.37 No.2

Cytolethal distending toxin(CDT)은 세포 주기 중 G2에서 M 기로의 전환을 막아 세포의 증식을 억제할 수 있는 세균 단백 독소의 일종이다. 구강 미생물 중 유일하게 Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans)만이 이 CDT를 생성 할 수 있는 것으로 알려져 있다. A. actinomycetemcomitans는 localized aggressive periodontitis (LAP)의 원인균으로 여겨지며 비 운동성의 그람 음성 구간균이고 $37^{\circ}C$, 5% $CO_2$ 하에 성장이 왕성하다. A. actinomycetemcomitans의 CDT는 3개의 인접한 유전자인 cdtA, cdtB, cdtC에 의해 형성 되며 각각의 유전자에 대한 단백질의 기능은 아직 완전히 밝혀지지 않았다. 현재까지 연구에 의하면 cdtA는 CDT의 세포부착과 관련이 있는 것으로 여겨지며 이 유전자의 기능 이상 시 CDT의 독성 효과가 현저히 감소한다고 알려져 있다. 따라서 본 연구는 A. actinomycetemcomitans의 cdtA 유전자를 클로닝, 단백질 발현하여 향후 치주질환의 발병 과정에서 CdtA의 역할을 규명하고 질환의 예방 및 치료법에 도움을 주고자 하였다. A. actinomycetemcomitans Y4균주를 cdtA 유전자 클로닝을 위해 사용하였다. A. actinomycetemcomitans의 genomic DNA는 genomic DNA 추출 kit를 사용하여 분리하고 cdtA에 특이적인 primer를 이용하여 PCR을 통해 cdtA 유전자를 증폭하였다. 증폭된 cdtA 유전자를 T-vector에 클로닝 하였으며, 클로닝 된 cdtA 유전자는 단백질 발현을 위해 pRSET Avector에 서브클로닝 한 후 발현 균주인 BL21(DE3)를 이용하여 발현시켰다. 발현 후 Ni-NTA AP conjugate를 이용한 Western blot을 통해 pRSET-CDTA를 확인하였다.

임플란트 시술 시 GBR의 실패 원인분석에 관한 후향적 연구

고선영,오준호,이승재,김형섭,Ko, Sun-Young,Oh, Jun-Ho,Lee, Seung-Jae,Kim, Hyung-Seop 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.3

Purpose: The aim of this retrospective study is to evaluate survival rate of implant and bone formation, to analyze failure contribution factor. Material and Methods: A total of 52 consecutive patients(35 male, 17 female, mean age 49 years) with 104 osseous defects were treated during the period from October 2004 to June 2007 with a simultaneous or staged GBR approach using non-resorbable or resorbable membranes combined with autogenous bone grafts or xenograft(Bio-Oss, Bio-cera, BBP). Result: A total of 32(30,8%) of 104 GBR-treated sites failed the bone formation and a total of 5(5.6%) of 89 implants were removed. Early exposure of the membrane has significantly affected bone formation(p<0.05). Non-resorbable membrane showed more exposure of the membrane and low success rate of bone formation than resorbable membrane(p<0.05). There were no difference between success rate of bone formation and using autogenous bone or graft materials. There were no statistically significant difference between success rate of bone formation and smoking or using PRP. Mandible showed more success rate of bone formation than maxilla(p<0.05). Conclusion: Early exposure of the membrane, membrane type and maxilla/mandible type have influence on success rate of bone formation during GBR.

보문 : 우리나라 의류상품 소매유통구조의 변화요인과 방향 (제1보)

고선영 ( Sun Young Ko ),이은영 ( Eun Young Rhee ) 韓國衣類學會 2006 한국의류학회지 Vol.30 No.11

퍼스널 모빌리티의 증가에 따른 이동환경 변화 연구

고선영(Ko, Sun Young) 한국디자인리서치학회 2017 한국디자인리서치 Vol.2 No.3

고령화와 1인 가구의 증대로 고가의 차량 보다는 1인용 이동수단에 대한 관심과 니즈가 늘어나고 있고 그에 따라 다양한 형태와 기능을 갖춘 퍼스널 모빌리티가 생겨나고 있다. 퍼스널 모빌리티는 저렴한 가격,기동성, 휴대성, 친환경적, 저렴 한 유지비용 등으로 사회적으로 큰 각광을 받고 있지만, 그에 따라 다양한 안전사고와 이용에 대한 문제점 등이 발생하고 있다. 어두운 밤에 이동하는 대리운전기사나 10대 어린아이들이 주로 사용하는 킥보드는, 공원이나 자전거 전용도로를 사 용할 수 없는 우리의 현 상황에서 차도만을 이용해야 하는 것은 무척이나 위험한 일이 아닐 수 없다. 유럽이나 싱가포르, 중국 등 주변 국가들은 이러한 세태에 발 빠르게 대응하여 그에 상응하는 법령과 적합한 기준을 제정하고 도로환경을 개 선하는 등의대처를 하고 있다.이러한 선행사례와 선행연구를 지침으로 우리나라 또한 모호한 기준과 법적 규정을 재정 비하여 우리나라의실정에 맞는 적합하고 합리적 방안을 마련해야 한다. 그와 동시에 협소하고 유지관리가 미흡한 도로 환경을 전반적으로 검토함으로써 퍼스널 모빌리티 이용자와 보행자, 차량이용자 등이 모두 쾌적하고 안전하게 도로를 이 용할 수 있는 개선방안을 마련해야 한다. 본 논문에서는 주변 국가들의 대응방안 선행사례와 선행연구를 통해 도로환경에 대한 개선점을제안함으로써 퍼스널 모빌리티 이용이 원활히 이루어질 수 있도록 하며 이로 인해 퍼스널 모빌리티에 대한 다양한 연구와 도로환경 개선에 대한 연구에 도움 되고자 한다.

보문 : 우리나라 의류상품 소매유통구조의 변화요인과 방향 (제2보)

고선영 ( Sun Young Ko ),이응영 ( Eun Young Rhee ) 한국의류학회 2010 한국의류학회지 Vol.34 No.7

플러스사이즈 여성과 일반여성의 패션쇼핑 서비스 만족도 영향요인 비교

고선영 ( Sun Young Ko ),유혜경 ( Hae Kyung Yu ),김찬주 ( Chan Ju Kim ) 한국의류학회 2015 한국의류학회지 Vol.39 No.1

This study analyzes the mechanism that explains how various service quality factors are related to consumersatisfaction when plus-size and regular-size women are engaged in fashion shopping. We constructedand tested service quality- perceived value-satisfaction model with size as a control variable. We definedplus-size women as those with a BMI over 25 and regular-size women below 25. Data were collected duringApril and May, 2012 and responses from 189 plus-size women and 246 regular-size women were used in thefinal analysis. The results are as follows. First, among service quality factors, attention was significantly relatedto perceived service value for both plus-size women and regular-size women. However, store facilitieswere significantly related to perceived service value for only plus-size women and kindness was significantlyrelated to perceived service value only in the case of regular-size women. Second, perceived servicevalue was significantly related to product satisfaction and service satisfaction for plus-size women and regular-size women. However a moderating effect was found between the groups where the influences of perceivedservice value on product satisfaction and service satisfaction in the case of plus-size women were greaterthan regular-size women. Third, in the case of regular-size women, service satisfaction was positively affectedby product satisfaction. However, the relationship between them was not found in the case of plus-size women.

보문 : 의복구매효능감이 소비자 만족 형성과정에 미치는 영향

고선영 ( Sun Young Ko ) 한국의류학회 2011 한국의류학회지 Vol.35 No.3

This study investigates the influence of self-efficacy in purchasing clothes in the consumer satisfaction formation process. A survey was conducted on 300 adults living in the Seoul and Gyeonggi area and 231 completed questionnaires were used for analysis. The survey questionnaires measured the respondent`s self-efficacy in purchasing clothes, expectation, perceived performance, expectation confirmation, and satisfaction level. The data were analyzed by moderated regression analysis. The results are as follows. First, for apparel products, perceived performance, and expectation confirmation have a direct effect on consumer satisfaction, while expectation has no direct effect on consumer satisfaction. This finding agrees with the results reported in a previous study on consumer goods with low ambiguity in assessing products and services including sneakers, underwear, cereals, and financial services. Second, the lower the self-efficacy in purchasing clothes, then the increased effect of the expectation confirmation that influences consumer satisfaction. However, the self-efficacy in purchasing clothes did not show any moderating effect on the perceived performance affecting the consumer satisfaction. The self-efficacy in purchasing clothes seems to have a moderating effect on the consumer satisfaction formation process by affecting the ambiguity in pre-purchase product assessment and not in post-purchase product evaluation.

여성 의복 구매의사결정과정에서 발생하는 오류의 원인과 유형

고선영 ( Sun-young Ko ) 한국소비자학회 2015 소비자학연구 Vol.26 No.6

구매하기는 했지만 거의 착용하지 않는 의복을 누구나 가지고 있다. 이 연구는 이러한 의복의 구매의사결정과정을 조사하여 구매실패의 원인을 밝히고 이를 유형화하고자 하였다. 또한 의복구매자가 이러한 오류를 인지하고 있는지를 알아보고 오류의 시정가능성을 파악하고자 하였다. 이를 위해 다양한 연령층의 21명의 여성을 심층 인터뷰하여 실패사례 수집하였고 이를 8가지로 유형화하였다. 평소 안 입던 새로운 스타일의 옷을 시도할 때, 이상적 자기를 추구하여 현실적으로 착용할 수 없는 옷을 구매할 때, 입어보지 않고 구매할 때, 급한 필요나 저가격으로 인해 마음에 안드는 옷을 구매할 때, 구매 시 자신에게 어울리는지 살펴보지 못한 경우, 구매 시 정보가 부족한 경우, 구매 시 주의가 부족한 경우, 마지막으로 구매 후 부정적인 경험이 각각 그것이다. 또한 의복구매자는 의사결정 오류를 어느 정도 인지하고 있었고 실패 경험을 통해 축적한 의사결정규칙을 가지고 있었다. 그러나 의복구매자는 이러한 선택규칙을 구매 상황에서 망각하거나 무시함으로써 구매의사결정 오류가 반복되었는데, 기분전환을 위한 충동구매, 다양성 추구, 이상적 자기 추구, 최적 구매에 대한 동기부족은 구매의사결정 오류를 지속시키는 중요한 요인으로 나타났다. This study was conducted on clothes rarely worn to identify and categorize the errors in the decision making process that led to their purchase. In addition, the study sought to understand the buyer’s perception of these errors and possibilities to correct errors. For this, in-depth interviews were conducted with a total of twenty-one women. The interviews were recorded and the transcripts were analyzed. The results are as follows. First, there are 8 failures types when the buyers make a decision to buy clothing. They are as follows: the buyers tried a new style that they do not typically wear; they bought clothes they can not physically wear in order to pursue an idealistic image; they bought the clothes without trying them on; they bought an item they did not like due to reasoning such as low pricing; they had little chance to confirm that the clothes fit well; they did not have enough information when purchasing the clothes; they were not careful when purchasing the clothes; and they had a negative experience after purchasing the clothes. Second, the buyers perceived their errors and tried to correct them through trial and errors. When they wanted a new style, they bought clothes that were not too far different from their previously purchased items and repeatedly bought more clothes of similar styles that fit them well to prevent failure. In addition, they understood that clothes should match their realistic image rather than their idealistic one to be more readily wearable. Buyers also thought they should try on clothes before buying them. They tried to reduce errors by identifying their preferences and confirming that the new clothes were suitable. Third, the buyers nevertheless repeatedly made errors when they forgot or neglected the rules accumulated from their buying experiences. Negative factors included ‘impulse buying to improve mood,’ ‘seeking out diversity,’ ‘seeking an idealistic image,’ and ‘lack of motivations for opportune buying.’

보문 : 의복구매효능감에 따른 의류제품평가의 차이

고선영 ( Sun Young Ko ) 한국의류학회 2011 한국의류학회지 Vol.35 No.7

This study examines whether there are differences in the evaluation of clothing products according to self-efficacy in purchasing clothes. A total of 300 surveys were distributed and 231 were used for analysis. The data were analyzed by regression and factor analysis. The results are as follows. First, there were significant differences in the product attributes considered according to self-efficacy in purchasing clothes. The consumers with high self-efficacy considered diverse product attributes comprehensively when making purchase decisions and had significantly higher score in perceived value and post-purchase satisfaction level than the consumers with low self-efficacy. Second, there were differences in the attributes of clothing products that effects perceived value according to self-efficacy in purchasing clothes. The attributes of ``clothes that I like`` and ``necessary clothes`` had a significant effect on perceived value for both consumers. The attributes of ``design that suits me`` and ``inexpensive price`` had significant effect on perceived value on consumers with high self-efficacy while the attributes of ``harmony with other clothes`` had a significant effect on perceived value for consumers with low self-efficacy. Third, there were differences in product attributes and the perceived value that influenced post-purchase satisfaction level according to self-efficacy in purchasing clothes. The attributes of ``design that suits me`` and perceived value had a direct effect on the post-purchase satisfaction level four consumers with a high self-efficacy. For the consumers with low self-efficacy, the attributes of ``clothes I like``, ``necessary clothes``, and ``design similar to that of usually purchased products`` had a direct effect on post-purchase satisfaction level; however, the effect of perceived value was not found.

산모 질환과 모유수유

고선영 ( Sun Young Ko ) 대한주산의학회 2005 Perinatology Vol.16 No.3