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1,3,5,7-Tetranitro-1,3,5,7-tetraazacyclooctane의 합성연구에 대한 고찰
강정부,구본탁,이상국,이경희,오동영,J. B. Kang,B. T. Koo,S. K. Lee,K. H. Lee,D. Y. Oh 대한화학회 1980 대한화학회지 Vol.24 No.4
1,3,5,7-Tetranitro-1,3,5,7-tetraazacyclooctane (homocyclonite)의 합성에 있어서 부산물로 1,3,5-trinitro-1,3,5-triazacyclohexane (cyclonite)이 동시에 생성되는데, 본 연구는 반응메카니즘 측면에서 주 생성물 homocyclonite로 가는 중간생성물인 1,5-endomethylene-3,7-dinitro-1,3,5,7-tetraazacyclooctane (DPT)을 액체크로마토그래피로 추적하고 반응계내의 총 메틸렌대 아미노 N 비율을 결정하기 위해 반응중에 첨가되는 paraformaldehyde의 최적의 양을 찾기 위한 이론적인 식을 유도하였으며, (식 1 참조) 실제 실험에서 얻어진 결과와 비교함으로서 주생성물인 homocyclonite의 최대의 수율을 얻기 위한 반응조건을 확인하였다. 1,3,5,7-Tetranitro-1,3,5,7-tetraazacyclooctane (homocyclonite) is prepared simultaneously with 1,3,5-trinitro-1,3,5-triazacyclohexane (cyclonite) by nitrolysis of hexamethylenetetramine. The purpose of our study was to detect the existence of intermediate, 1,5-endomethylene-3,7-dinitro-1,3,5,7-tetraazacyclooctane (DPT), by liquid chromatography based on the reaction mechanism, to derive the theoretical equation which is determinated by the ratio of total methylene group vs. amino N, for the proper quantity of paraformaldehyde added in the reaction and to obtain the optimum condition for maximum yield and purity by comparing the experimental results with the theoretical equation.
강정부,안병홍,고영두 ( Chung Boo Kang,Byung Hong Ahn,Young Du Ko ) 한국축산학회 1986 한국축산학회지 Vol.28 No.8
This survey was carried out to investigate the present situation of feeding management and economical analysis of dairy farms in Gyeong Nam province from June 25, 1985 to May 31, 1986. The survey was divided into three herd size according to the number of dairy cattle such as small (10 to 30 head farm), medium (30 to 70 head per farm) and large size (over 70 head per farm). Number of dairy farms surveyed was 20, 20 and 10 in the small, medium and large herd size, respectively. The results obtained are as follows: 1. Age of farm owner was largely between 40 and 50 years old (about 50%) and the ages were not affected by the herd size. Academic career of farm owner was related to the herd size: large herd size was largely owned by university/college graduates (80%) and small-medium herd size by high school graduates (50%). 2. Small farms were managed by farm owners while over 80% of medium and large farms were managed by the employed managers. 3. Areas for forage crop field/pasture per head increased with increasing herd size: 4.9 a in small, 11.8 a in medium and 20.1 a in large. About 45 to 64% of small and medium herd size have silo but silo were very limitedly utilized. All of large herd size silo but 70% of silo were actually utilized. Farm facilities such as calving pen and waste disposer were not available to small and medium herd size. Only a half of large of herd size were utilized with those facilities. 4. Dairy farms were not utilized artificial insemination only. Largely a half of large farms utilized both artificial insemination and natural coitus. 5. Administration of magnet was neglected by small herd size but it was practised by medium (40%) and large (60%) herd size. 6. Dairly concentrates intake of milking cows was 8.6Kg in small and large herd size, and 9.6Kg in medium herd size. 7. Daily milk yield per herd was 14.6Kg in small, 16.4Kg in medium and 15.7Kg in large herd size. 8. The intake of nutrients of dairy cattle such as crude protein, calcium and phosphorus was higher than that of NRC requirements. 9. Feed cost by herd size took 55%, 56%, and 54% of milk price, respectively. The cost of concentrates composed about 67% of the total feed costs.
사염화탄소(CCl$_{4}$)의 투여가 쥐의 간기능에 미치는 영향 2. 혈청 효소 활성치
강정부,이은석,허주헝 한국임상수의학회 1997 한국임상수의학회지 Vol.14 No.2
Several serum enzyme activities were measured after intraBeritoneal administration of 0.01 ml $CCl_{4}$ per 100 g of body weight in Sprague Dawley rats. Senun AST activity increased significantly after administration of CCl$_{4}$ (P<0.05). The serum AST activity at 2 hours after $CCl_{4} administration (475 {\pm} 10^{6} IU/L)$ was significantly higher than that of control group $(65 {\pm} 14 IU/L)$. The high level of serum AST activity maintained up to 48 hours. Serum ALT activity in $CCl_{4}$-treated groups was also significantly higher from 4 hours after treatment companied to control group and the high level maintained up to 48 hours. Serum ALP and ${\gamma} GTP activities in CCl_{4}$-treated groups were significantly higher from 8 hours after treatment compared to control group and the level maintained up to 48 hours.
강정부,신종욱,Kang Chung-Boo,Shin Jong-Uk 대한수의사회 1985 대한수의사회지 Vol.21 No.6
This experiment was performed to investigate the levels of hematological and blood chemical parameters in korean native growing cattle from 7 month to 11 month after birth. Animals used were 15 healthy korean native growing cattle with about 190kg body we
실험동물에서 Apoptosis의 모델개발과 생체면역반응 및 형태학적 특징 I. Apoptosis 및 Hepatic Tumorigenesis의 유도 및 관련지표의 검색
강정부,하우송,김지경 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.1
Apoptosis is now widely recognized as a common form of cell death and represents mechanism of cell clearance in many physiological situations where deletion of cells is required. In vivo administration of bacterial lipopolysaccharide (LPS) to Balb/c mice induced DNA fragmentation in the thymus. DNA fragmentation in the thymus was roughly dependent on the dose of LPS injected and reached the peak 18 hours after injection. This apoptosis in the thymus might be mediated due to LPS stimulant. DEN (diethylnitrosamine) has been shown to cause liver cancer in experimental animals and humans. The hepatic tumorigenesis was induced by ad libitum feeding of DEN only. It was suggested that DEN induced hepatic tumorgenesis in rat is a good reproducible model for studying biochemical and pathophysiological changes associated with the development of hepatic tumorigenesis and apoptosis.
2,2'-Azobis (2- amidinopropano) Dihydrochloride(AAPH)의 투여가 쥐의 간기능에 미치는 영향 1. 임상증상 및 혈액 화학치 소견
강정부,손호상,김철호 한국임상수의학회 1998 한국임상수의학회지 Vol.15 No.1
This study was performed to estimate the clinical signs and changes of biochemical parameters in rats with hepatic injury induced by the administration of 2, 2'-azobis(2- amidinopropane)dihydrochloride (AAPH) . Minor behavioral change, brittleness of skin hair and decreased volume of water and feed intake were observed in ra% 2 hours after AAPH administration compared to control group. Concentration of serum albumin showed lower than that of control group. However, concentration of total bilirubin has shown higher than that of control group. As time goes on, . serum LDH activity increased significantly compared to control group, but senun CPK activity did not change compared to control group. Passive hemanglutination of $\alpha $-fetoprotein was negative in all the treaDent groups and control group.